Current Research in Microbial Sciences最新文献

{"title":"Evaluation and identification of metabolites produced by Cytobacillus firmus in the interaction with Arabidopsis thaliana plants and their effect on Solanum lycopersicum","authors":"Itzel G. Arteaga-Ríos ,&nbsp;Karen Beatriz Méndez-Rodríguez ,&nbsp;Raul Ocampo-Pérez ,&nbsp;María de la Luz Guerrero-González ,&nbsp;Raúl Rodríguez-Guerra ,&nbsp;Pablo Delgado-Sánchez","doi":"10.1016/j.crmicr.2024.100312","DOIUrl":"10.1016/j.crmicr.2024.100312","url":null,"abstract":"<div><div>Currently, the use of bio-inputs is increasing due to the need to reduce the use of agrochemicals. However, one of the limitations is to preserve the viability of the living microorganisms, so it is important to find an alternative that allows us to obtain different metabolites to produce it. We evaluated three different interactions (contact, diffusible and volatile compounds) <em>in vitro</em> in <em>Arabidopsis thaliana</em> (At) seedlings with the strain <em>Cytobacillus firmus</em> M10 and its filtered secondary metabolites (M10F). The results showed that the seedlings inoculated by contact with the filtrate (AtM10F) presented increases in root length (30 %) and leaf area (33 %), as well as in the volatile interaction (At/M10F) with respect to the uninoculated treatment. For both interactions, the seedlings inoculated with the bacteria by contact (AtM10) and volatile (At/M10) obtained greater biomass (48 and 57 %). Subsequently, an evaluation at the end of the <em>A. thaliana</em> cycle showed that the treatments obtained by contact and distance when reinoculated with the bacteria and the filtrate (AtM10, At-M10 and AtM10F) obtained 50 % more seed yield than the control treatment, while AtM10F presented 72 %, while At/M10F presented the highest no. of siliques and seeds, which increased the yield by 65 %. In the <em>Solanum lycopersicum</em> (Sl) experiment, the filtrate (SlM10F) showed significant differences in seedling height, leaf length and width (23, 24 and 36 %, respectively). It also promoted an increase in fresh and dry weight, producing a greater root area and larger leaves compared to the control (Sl) and the bacteria (SlM10). We performed a qualitative characterization of the secondary metabolites present in the filtrate, where we found 2,4-DTBP, sylvopinol, isophthaladehyde, and eicosane of interest with possible growth-promoting effects on <em>A. thaliana</em> and tomato. We identified volatile compounds present in plant-microorganism and plant-filtrate interactions as possible precursors in the induction of plant growth, among which phenols, alcohols, aldehydes, alkanes, and alkenes stand out. Most of the analyzed compounds have not been found in the literature with reports of growth promoters, is important to mention that due to their characteristic functional groups they can derive and trigger the synthesis of new molecules with agronomic application.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100312"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in Acinetobacter baumannii","authors":"Sirui Zhang ,&nbsp;Jingdan Wang ,&nbsp;Rong Yu ,&nbsp;Haiping Liu ,&nbsp;Shuyan Liu ,&nbsp;Kai Luo ,&nbsp;Jin'e Lei ,&nbsp;Bei Han ,&nbsp;Yanjiong Chen ,&nbsp;Shaoshan Han ,&nbsp;E Yang ,&nbsp;Meng Xun ,&nbsp;Lei Han","doi":"10.1016/j.crmicr.2024.100332","DOIUrl":"10.1016/j.crmicr.2024.100332","url":null,"abstract":"<div><div>Although various mechanisms of carbapenem-resistance have been identified in the nosocomial pathogen <em>Acinetobacter baumannii</em>, the critical process of resistance evolution and the factors involved in are not well understood. Herein, we identified a universal stress protein Usp1413 which played an important role in adaptive resistance of <em>A. baumannii</em> to meropenem (MEM). Based on RNA-Seq and genome sequencing, Usp1413 was not only one of the most downregulated USPs, but also the bare one having mutation of tyrosine and glycine inserted at the site of 229-230 (YG229-230) under the stimulation of MEM. Deletion of Usp1413 resulted in increased MEM resistance. In addition, Usp1413 affected the bacterial abilities of biofilm formation and swarm motility, as well as helped <em>A. baumannii</em> response to various environmental stresses. These effects of Usp1413 were achieved by regulating its interaction proteins, within the functions of YigZ family protein, acetyltransferase, and SulP family inorganic anion transporter. The insertion mutation of YG229-230 influenced both the expression of interaction proteins and the phenotypes of bacteria. Finally, the promotor region of Usp1413 was convinced by point mutations. Overall, our findings identified the universal stress protein Usp1413 as a contributor involved in MEM adaptive resistance and responded to numerous environmental stresses. This study provides novel insights into the mechanism of universal stress proteins in participating antibiotic resistance, and affords a potential target for controlling drug resistance development in <em>A. baumannii</em>.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100332"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of carotenoid-producing Rhodotorula yeasts from amazonian substrates for sustainable biotechnology applications","authors":"Raissa Sayumy Kataki Fonseca ,&nbsp;Kevyn Melo Lotas ,&nbsp;Ana Claudia Alves Cortez ,&nbsp;Flávia da Silva Fernandes ,&nbsp;Érica Simplício de Souza ,&nbsp;Laurent Dufossé ,&nbsp;João Vicente Braga de Souza","doi":"10.1016/j.crmicr.2025.100373","DOIUrl":"10.1016/j.crmicr.2025.100373","url":null,"abstract":"<div><div>Carotenoids are natural pigments responsible for the yellow, orange, and red colors seen in various organisms. The aim of this study was to investigate the potential of yeast strains belonging to the genus <em>Rhodotorula</em> isolated from the Amazon Region in the production of carotenoids. Environmental samples from the Amazon Region were collected for yeast isolation. Isolates showing pigmented colonies underwent morphological and biochemical studies, as well as assessment of their potential for carotenoid production. The three best producers were identified through nucleotide sequencing of the ITS1–5.8s-ITS4 rDNA region. The top producer underwent univariate experiments to evaluate the influence of different C/N sources. Carotenoids produced were evaluated using CCD. Results showed the isolation of 13 pigmented yeasts with morphological and biochemical characteristics consistent with the genus <em>Rhodotorula.</em> Isolates RGM42 (601 μg/g), RTC42 (362 μg/g), and RTC45 (351 μg/g) stood out as the top carotenoid producers. These yeasts were identified as belonging to the species <em>Rhodotorula mucilaginosa</em>. Regarding C/N influence, <em>R. mucilaginosa</em> RGM42 produced the highest number of carotenoids using glucose and peptone as carbon and nitrogen sources, respectively. Isolate <em>R. mucilaginosa</em> RGM42 exhibited maximum growth with a peak at 72 h of bioprocess. Extracts from isolates <em>R. mucilaginosa</em> RGM42, <em>R. mucilaginosa</em> RTC42, <em>and R. mucilaginosa</em> RTC45 showed retention indices like β-carotene in thin-layer chromatography; however, <em>R. mucilaginosa</em> RGM42 presented an additional pigment in its chromatographic profile.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100373"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaken not stirred - effect of different mixing modes during the cultivation of methanogenic pure cultures","authors":"Andja Mullaymeri,&nbsp;Maria Payr,&nbsp;Mathias Wunderer,&nbsp;Eva Maria Eva Maria,&nbsp;Andreas Otto Wagner","doi":"10.1016/j.crmicr.2025.100386","DOIUrl":"10.1016/j.crmicr.2025.100386","url":null,"abstract":"<div><div>Numerous cultivation techniques for aerobic microorganisms have been extensively investigated in the field of microbiology. Optimisation of these techniques is important for scientific and economic reasons. Methanogenic archaea, however, are obligate anaerobic microorganisms requiring different cultivation techniques than aerobic organisms due to the fundamental differences in physiology. Mixing of aerobic cultures is generally considered as very important as it provides organisms with essential oxygen; however, for anaerobic microorganisms lacking the ability to grow with oxygen, this point in cultivation was widely neglected. This work aimed at investigating the effect of different mixing modes on cultures of the methanogenic archaea <em>Methanomethylovorans thermophila, Methanosarcina acetivorans, Methanosarcina thermophila</em> and <em>Methanococcus vannielii</em> by cultivating them anaerobically in the modes standing/lying, shaken/unshaken and large/small serum flask in order to analyse their impact on the methane and biomass production. This study showed that a shaken incubation mode had a positive impact on methane production and resulted in its accelerated production, especially in hydrogenotrophic cultures; however, higher methane production did not necessarily lead to higher biomass production.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100386"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kodamaea ohmeri: An emergent yeast from a One Health perspective","authors":"Sthefany Emanuelle Silva ,&nbsp;Lorena Souza Silva ,&nbsp;Ludmila Gouveia Eufrasio ,&nbsp;Gabriela Silva Cruz ,&nbsp;Fabíola Lucini ,&nbsp;Hareton Teixeira Vechi ,&nbsp;Manoella do Monte Alves ,&nbsp;Luciana Rodrigues Ferreira Ribeiro ,&nbsp;Karine Lilian de Souza ,&nbsp;José Aparecido Moreira ,&nbsp;Janete Gouveia de Souza ,&nbsp;Florent Morio ,&nbsp;Gisela Lara da Costa ,&nbsp;Barbara de Oliveira Baptista ,&nbsp;Luiz Marcelo Ribeiro Tomé ,&nbsp;Sílvia Helena Sousa Pietra Pedroso ,&nbsp;Felipe Campos de Melo Iani ,&nbsp;Talita Émile Ribeiro Adelino ,&nbsp;Débora Castelo-Branco ,&nbsp;Luana Rossato ,&nbsp;Rafael Wesley Bastos","doi":"10.1016/j.crmicr.2025.100359","DOIUrl":"10.1016/j.crmicr.2025.100359","url":null,"abstract":"<div><div><em>Kodamaea ohmeri</em> is an emerging and opportunistic yeast associated with a high mortality rate in humans. As it is commonly found in the environment, it is possible that environmental conditions and agricultural practices contribute to the adaptation of this yeast and the selection of antifungal resistance. During a multicentric study in Brazil, conducted under a One Health perspective, 14 isolates of <em>K. ohmeri</em> were identified from different sources: three from blood cultures, three from animals (swine and poultry), and eight from animal environments (swine and poultry). Yeasts were isolated using CHROmagar® <em>Candida</em> medium and identified by MALDI-TOF MS and ITS rDNA barcoding. Minimum inhibitory concentration (MIC) was determined using the broth microdilution method for clinical (azoles, echinocandins, pyrimidine analogs, and polyenes), and environmental antifungals (tebuconazole, pyraclostrobin, carbendazim, and mancozeb), and hospital disinfectants (quaternary ammonium compounds). Of note, color variations of <em>K. ohmeri</em> were noted on CHROmagar® depending on the incubation time, which is likely to complicate its identification. Following polyphasic identification and taxonomic confirmation, all isolates demonstrated low MIC values for clinical antifungals, disinfectants, and tebuconazole. However, all isolates were able to grow in the presence of carbendazim, mancozeb, and pyraclostrobin. Together, these findings highlight the risks associated with the use of environmental azoles, such as tebuconazole, as they may impact non-target fungi of medical importance, but other fungicides do not present the same risk. This is the first study to demonstrate that <em>K. ohmeri</em>, an important emerging yeast in human medicine, can be isolated from various sources, including patients. Although the isolates exhibited low MIC values for clinical antifungals, it is crucial to monitor changes in sensitivity patterns over time in emerging microorganisms to prevent the development of multidrug resistance, which may originate in the environment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100359"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of isoprene by soil Actinomycetota from coffee-tea integrated plantations in a tropical evergreen forest","authors":"Toungporn Uttarotai ,&nbsp;Terry J. McGenity ,&nbsp;Sawannee Sutheeworapong ,&nbsp;Wuttichai Mhuantong ,&nbsp;Nuttapon Khongdee ,&nbsp;Sakunnee Bovonsombut ,&nbsp;Thararat Chitov","doi":"10.1016/j.crmicr.2025.100382","DOIUrl":"10.1016/j.crmicr.2025.100382","url":null,"abstract":"<div><div>Isoprene, a biogenic volatile compound emitted largely by plants, can form greenhouse gases when it reacts with atmospheric radicals. A significant amount of isoprene is absorbed into soil and can be degraded by soil microorganisms, but our understanding of the microbial biodegradation of isoprene in tropical ecosystems remains limited. This study investigated isoprene degradation by soil microbes indigenous to a tropical evergreen forest, focusing on those associated with coffee and tea plants grown as integrated crops and their genome characteristics in relation to their biodegradation capabilities. Following a 96-hour incubation with 7.2 × 10⁵ parts per billion by volume (ppbv) of isoprene, soil samples exhibited degradation levels ranging from 11.95 % to 36.54 %. From these soils, bacterial isolates belonging to the genera <em>Rhodococcus</em> and <em>Gordonia</em> (Actinomycetota) were recovered. These isolates demonstrated high isoprene biodegradation activity (50.3 %–69.1 % over seven days) and carried the <em>isoA</em> gene associated with isoprene metabolism. According to genome analysis, the organization of genes in the <em>iso</em> cluster was homologous, and the encoded amino acid sequences were highly similar to those of previously known isoprene-degrading members of the same genera. These findings emphasized the contribution of these widespread isoprene-degrading bacterial genera in the biodegradation of isoprene and the role of their isoprene monooxygenases in modulating atmospheric isoprene flux.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100382"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus dynamics and sustainable agriculture: The role of microbial solubilization and innovations in nutrient management","authors":"José Abraham García-Berumen ,&nbsp;Juan Armando Flores de la Torre ,&nbsp;Sergio de los Santos-Villalobos ,&nbsp;Alejandro Espinoza-Canales ,&nbsp;Francisco Guadalupe Echavarría-Cháirez ,&nbsp;Héctor Gutiérrez-Bañuelos","doi":"10.1016/j.crmicr.2024.100326","DOIUrl":"10.1016/j.crmicr.2024.100326","url":null,"abstract":"<div><div>Phosphorus (P) is an essential element for plant growth, playing a crucial role in various metabolic processes. Despite its importance, phosphorus availability in soils is often restricted due to its tendency to form insoluble complexes, limiting plant uptake. The increasing demand for phosphorus in agriculture, combined with limited global reserves of phosphate rock, has created challenges for sustainable plant production. Additionally, the overuse of chemical phosphorus fertilizers has resulted in environmental degradation, such as eutrophication of water bodies. Increasing agronomic phosphorus (P) efficiency is crucial because of population growth and increased food demand. Hence, microorganisms involved in the P cycle are a promising biotechnological strategy that has gained global interest in recent decades. Microorganisms' solubilization of phosphate rock (PR) is an environmentally sustainable alternative to chemical processing for producing phosphate fertilizers. Phosphorus-solubilizing microorganisms (PSMs), including bacteria and fungi, and their enzymatic processes offer an eco-friendly and sustainable alternative to chemical inputs by converting insoluble phosphorus into forms readily available for plant uptake. Integrating PSMs into agricultural systems presents a promising strategy to reduce dependence on chemical fertilizers, enhance soil health, and contribute to the transition toward more sustainable and resilient agricultural practices. It can be an alternative that reduces the loss of phosphorus in the environment, especially the eutrophication of aquatic systems. This paper explores the challenges of phosphorus availability in agriculture and the potential of microbial phosphorus solubilization as a sustainable alternative to conventional practices.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100326"},"PeriodicalIF":4.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering latent infections in kidneys: A novel molecular approach for differential Leptospira detection","authors":"Li-Fang Chou ,&nbsp;Yi-Chun Liu ,&nbsp;Huang-Yu Yang ,&nbsp;Ya-Chung Tian ,&nbsp;Chih-Ho Lai ,&nbsp;Ming-Yang Chang ,&nbsp;Cheng-Chieh Hung ,&nbsp;Tong-Hong Wang ,&nbsp;Shen-Hsing Hsu ,&nbsp;Chung-Ying Tsai ,&nbsp;Pei-Yu Hung ,&nbsp;Chih-Wei Yang","doi":"10.1016/j.crmicr.2024.100327","DOIUrl":"10.1016/j.crmicr.2024.100327","url":null,"abstract":"<div><div>Leptospirosis, a re-emerging zoonotic disease caused by <em>Leptospira</em> spp., poses significant global health and veterinary challenges. Long-term colonization of renal tubules by <em>Leptospira</em> in asymptomatic hosts highlights the need for sensitive detection methods. This study evaluates the chronic or latent <em>Leptospira</em> infections in kidneys using a novel molecular approach to examine individual immune responses differences. Digital PCR strategies employing newly developed primer-probe sets targeting the flagellar <em>fliG</em> gene were used to assess the presence of trace <em>Leptospira</em> in infected murine kidneys and urine samples from laboratory-confirmed leptospirosis patients. RNA-based digital PCR detected leptospires in 58 % (targeting <em>lipl32</em>) and 83 % (targeting <em>fliG</em>) of infected kidneys, demonstrating that the digital PCR strategy targeting the <em>fliG</em> gene offers superior sensitivity. Notably, the newly developed <em>fliG</em>-targeting assay detected as low as 20 fg of <em>Leptospira</em> DNA, offering ten-fold greater sensitivity than traditional qPCR for trace detection. This allows for differential detection of <em>Leptospira</em> species and facilitates monitoring of extremely low bacterial loads with greater sensitivity than conventional methods. We also observed regenerating renal tubules with mitosis and elevated cytokine expression in kidneys with transcriptionally active <em>Leptospira</em> during chronic infection. This approach aids in identifying latent infections and offers insights into individual variations. Our research provides a powerful molecular tool for epidemiological studies and public health surveillance, contributing valuable insights into the prevalence and transmission dynamics of this pervasive zoonotic disease.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100327"},"PeriodicalIF":4.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere microbiome regulation: Unlocking the potential for plant growth","authors":"Chenghua Luo ,&nbsp;Yijun He ,&nbsp;Yaping Chen","doi":"10.1016/j.crmicr.2024.100322","DOIUrl":"10.1016/j.crmicr.2024.100322","url":null,"abstract":"<div><div>Rhizosphere microbial communities are essential for plant growth and health maintenance, but their recruitment and functions are affected by their interactions with host plants. Finding ways to use the interaction to achieve specific production purposes, so as to reduce the use of chemical fertilizers and pesticides, is an important research approach in the development of green agriculture. To demonstrate the importance of rhizosphere microbial communities and guide practical production applications, this review summarizes the outstanding performance of rhizosphere microbial communities in promoting plant growth and stress tolerance. We also discuss the effect of host plants on their rhizosphere microbes, especially emphasizing the important role of host plant species and genes in the specific recruitment of beneficial microorganisms to improve the plants’ own traits. The aim of this review is to provide valuable insights into developing plant varieties that can consistently recruit specific beneficial microorganisms to improve crop adaptability and productivity, and thus can be applied to green and sustainable agriculture in the future.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100322"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of extracellular chitin deacetylase from Aneurinibacillus aneurinilyticus isolated from marine crustacean shell","authors":"Poonam Das ,&nbsp;Manisha Das ,&nbsp;Sheela Kumari Sahoo ,&nbsp;Jagneshwar Dandapat ,&nbsp;Jyotsnarani Pradhan","doi":"10.1016/j.crmicr.2024.100325","DOIUrl":"10.1016/j.crmicr.2024.100325","url":null,"abstract":"<div><div>Chitosan is a promising biopolymer with wide range of applications. It is the deacetylated product of chitin. Commercially, it is produced from chitin via a harsh thermochemical process that has several shortcomings and heterogenous deacetylation product. Chitin can be transformed into chitosan through enzymatic deacetylation using chitin deacetylase (CDA), enabling the production of chitosan with a specific degree of deacetylation. CDA is primarily extracted from fungi followed by bacteria and insects. The extraction of CDA from fungus is more complex, possess several health risks for human including skin lesions. Therefore, screening of potent bacterial CDA is the need of the hour. In this study, for the first time we have isolated a bacterial strain Aneurinibacillus aneurinilyticus from the rinsed water of marine crab shell, and it was found to be a potent CDA producer. The extracellular CDA from <em>A. aneurinilyticus</em> has been partially purified and the specific activity of the enzyme was found to be 569.73 U/ mg protein. SDS-PAGE profiling of the purified sample depicts two isomers of CDA with molecular weights of 27 kD and 45 kD. The pH and temperature optima of the purified CDA were found to be 7.4 and 37 °C, respectively. The partially purified enzyme has Km and Vmax values of 98.455 µM and 909.09 µmole/min, for non-chitinous substrate such as p-nitroacetanilide. For chitinous substrates like glycol chitin, N-acetyl glucosamine hexamer and colloidal chitin, the enzyme exhibited K_m of 96.96, 111.75 and 127.86 µM, respectively, V_max for these substrates were 23.31, 10.12 and 10.772 µmole/min, respectively. Metal ions like Mn and Mg considerably boost the production and activity of CDA, whereas Cd and Co strongly inhibit its activity. Insights from this study further substantiate that this enzyme follows Michaelis-Menten equation and has potential for industrial applications.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100325"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}