Current Research in Microbial Sciences最新文献

{"title":"Four recent insights suggest the need for more refined methods to assess the resistogenicity of doxycycline post exposure prophylaxis","authors":"Thibaut Vanbaelen ,&nbsp;Sheeba Santhini Manoharan-Basil ,&nbsp;Chris Kenyon","doi":"10.1016/j.crmicr.2024.100234","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100234","url":null,"abstract":"<div><p>Two recently published randomized trials of doxycycline post exposure prophylaxis (PEP) have concluded that this intervention is highly effective at reducing the incidence of bacterial sexually transmitted infections (STIs) and has little or no risk of promoting the spread of antimicrobial resistance (AMR). In this perspective piece, we review four types of evidence that suggest that the risk of promoting AMR has been inadequately assessed in these studies. 1) The studies have all used proportion resistant as the outcome measure. This is a less sensitive measure of resistogenicity than MIC distribution. 2) These RCTs have not considered population-level pathways of AMR selection. 3) In populations with very high antimicrobial consumption such as PrEP cohorts, the relationship between antimicrobial consumption and resistance may be saturated. 4) Genetic linkage of AMR means that increased tetracycline use may select for AMR to not only tetracyclines but also other antimicrobials in STIs and other bacterial species. We recommend novel study designs to more adequately assess the AMR-inducing risk of doxycycline PEP.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100234"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000166/pdfft?md5=06f8c2dee8e4ef0a6dbc48757ae845af&pid=1-s2.0-S2666517424000166-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140555104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the impact of Rapamycin on the virulence factors of the Candida haemulonii complex","authors":"Vinicius Alves ,&nbsp;Iara Bastos de Andrade ,&nbsp;Dario Corrêa-Junior ,&nbsp;Igor Avellar-Moura ,&nbsp;Karini Passos ,&nbsp;Juliana Soares ,&nbsp;Bruno Pontes ,&nbsp;Marcos Abreu Almeida ,&nbsp;Rodrigo Almeida-Paes ,&nbsp;Susana Frases","doi":"10.1016/j.crmicr.2024.100247","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100247","url":null,"abstract":"<div><p>The incidence of invasive fungal infections caused by <em>Candida</em> species is increasing, particularly in immunocompromised individuals. This increasing incidence poses a dual challenge, comprising escalating antifungal resistance and the necessity for accurate fungal identification. The <em>Candida haemulonii</em> complex further complicates these challenges due to limited identification tools. Like some other <em>Candida</em> species, infections involving this complex show resistance to multiple antifungals, requiring innovative therapeutic approaches. Rapamycin, known for its antifungal properties and immunosuppressive characteristics, was investigated against the <em>C. haemulonii</em> complex species. Results revealed a rapamycin minimal inhibitory concentration (MIC) range of 0.07 to &gt;20 µM, with fungicidal effects in most strains. <em>In vitro</em> analyses using the rapamycin maximum plasma concentration (0.016 µM) showed reduced surface properties and decreased production of extracellular enzymes. Rapamycin also hindered biofilm formation by some strains. Even when treated at the human therapeutic dose, which is lower than the MIC, phenotypic variations in <em>C. haemulonii</em> were detected, hinting at the possible attenuation of some virulence factors when exposed to rapamycin.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100247"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000294/pdfft?md5=3cec5c16c2f4c2806e49152b1b0bed8c&pid=1-s2.0-S2666517424000294-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141314118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in microbial based bio-inoculum for amelioration of soil health and sustainable crop production","authors":"Aurodeepa Samantaray ,&nbsp;Sourav Chattaraj ,&nbsp;Debasis Mitra ,&nbsp;Arindam Ganguly ,&nbsp;Rahul Kumar ,&nbsp;Ashish Gaur ,&nbsp;Pradeep K.Das Mohapatra ,&nbsp;Sergio de los Santos-Villalobos ,&nbsp;Anju Rani ,&nbsp;Hrudayanath Thatoi","doi":"10.1016/j.crmicr.2024.100251","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100251","url":null,"abstract":"<div><p>The adoption of sustainable agricultural practices is increasingly imperative in addressing global food security and environmental concerns, with microbial based bio-inoculums emerging as a promising approach for nurturing soil health and fostering sustainable crop production.This review article explores the potential of microbial based bio-inoculumsor biofertilizers as a transformative approach toenhance plant disease resistance and growth. It explores the commercial prospects of biofertilizers, highlighting their role in addressing environmental concerns associated with conventional fertilizers while meeting the growing demand for eco-friendly agricultural practices. Additionally, this review discusses the future prospects of biofertilizers, emphasizing the ongoing advancements in biotechnology and formulation techniques that are expected to enhance their efficacy and applicability. Furthermore, this article provides insights into strategies for the successful acceptance of biofertilizers among farmers, including the importance of quality control, assurance, and education initiatives to raise awareness about their benefits and overcome barriers to adoption. By synthesizing the current research findings and industrial developments, this review offers valuable guidance for stakeholders seeking to exploit the potential of biofertilizers or beneficial microbes to promote soil health, ensure sustainable crop production, and addressing the challenges of modern agriculture.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100251"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000336/pdfft?md5=c7ec37bf42a93fd4b79bc3756f732c57&pid=1-s2.0-S2666517424000336-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of Rhizospheric and Endophytic Bacteria from Cucumber plants irrigated with wastewater: Exploring their roles in plant growth promotion and disease suppression","authors":"Kumar Shreshtha ,&nbsp;Satyam Raj ,&nbsp;Arun Kumar Pal ,&nbsp;Pooja Tripathi ,&nbsp;Krishna Kumar Choudhary ,&nbsp;Debasis Mitra ,&nbsp;Anju Rani ,&nbsp;Sergio de los Santos-Villalobos ,&nbsp;Vijay Tripathi","doi":"10.1016/j.crmicr.2024.100256","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100256","url":null,"abstract":"<div><p>Wastewater contains various emerging contaminants, including heavy metals, residues of pesticides, and pharmaceuticals. Therefore, irrigation with wastewater can enhance heavy metal contamination in soil and adversely affect plant growth. To mitigate this problem, plant growth-promoting bacteria (PGPR) can improve plant growth under heavy metal stress. This study aimed to isolate and characterize rhizospheric and endophytic bacteria from the rhizosphere soil and roots of a cucumber plant irrigated with municipal wastewater. A total of 121 morphologically distinct bacterial isolates from the rhizosphere and 90 bacterial isolates from the endophytic region were isolated and tested for heavy metal resistance and <em>in vitro</em> plant growth-promoting characteristics, including indole-3-acetic acid (IAA) production, phosphate solubilization, Hydrogen Cyanide (HCN) production, and siderophore production. Most of the bacteria analyzed from the rhizospheric and endophytic regions showed various plant growth-promoting characteristics and were tolerant to different heavy metals at various concentrations. Bacterial strains R1 <em>(Proteus</em> sp.<em>)</em> and E2 (<em>Bacillus</em> sp.<em>)</em> were antagonistic to <em>Fusarium oxysporum</em> f. sp. <em>Lycopersici.</em> Wastewater irrigation increases heavy metal-resistant bacteria in cucumber plants, which can alleviate heavy metal stress. Additionally, <em>Proteus</em> sp<em>.</em> and <em>Bacillus</em> sp<em>.</em> isolates are potential candidates for removing heavy metal-contaminated soil and could be potential biofertilizer candidates for selected plants and biocontrol agents.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100256"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000385/pdfft?md5=75a063de4c9c6ccdff4b00d4e5d040e9&pid=1-s2.0-S2666517424000385-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agro-industrial waste to microbial sophorolipids: Innovations and impacts on the food industry","authors":"Aishwary Purohit ,&nbsp;Amar Jyoti Das ,&nbsp;Debashish Ghosh","doi":"10.1016/j.crmicr.2024.100275","DOIUrl":"10.1016/j.crmicr.2024.100275","url":null,"abstract":"<div><div>The cost-effective production of sophorolipids (SLs) using agro-industrial waste represents a significant advancement in sustainable practices within the food industry. Sophorolipids, known for their excellent emulsifying and antimicrobial properties, offer a promising natural alternative to synthetic preservatives, which can pose health and environmental risks. This study aims to critically assess the strategies for producing sophorolipids from agro-industrial waste, with a focus on their implications for improving food safety and quality. By integrating techno-economic analysis (TEA) and life cycle assessment (LCA), the review provides a comprehensive evaluation of the effectiveness, feasibility, and sustainability of these methods. The scientific importance of this research lies in its potential to enhance waste management practices and promote eco-friendly food preservation solutions, contributing to the development of safer and more sustainable industrial processes.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100275"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Unravelling the diversity of Anaplasma species circulating in selected African wildlife hosts by targeted 16S microbiome analysis” [Current Research in Microbial Sciences Volume 5 (2023) 100198]","authors":"S. Marcus Makgabo ,&nbsp;Kelly A. Brayton ,&nbsp;Marinda C. Oosthuizen ,&nbsp;Nicola E. Collins","doi":"10.1016/j.crmicr.2024.100279","DOIUrl":"10.1016/j.crmicr.2024.100279","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100279"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpaca-derived nanobody targeting the hydrophobic pocket of the HIV-1 gp41 NHR broadly neutralizes HIV-1 by blocking six-helix bundle formation","authors":"Lujia Sun ,&nbsp;Bo Chen ,&nbsp;Xianbo Liu ,&nbsp;Yun Zhu ,&nbsp;Guangxu Zhang ,&nbsp;Xiaoxing Liang ,&nbsp;Lixiao Xing ,&nbsp;Wei Xu ,&nbsp;Shibo Jiang ,&nbsp;Xinling Wang","doi":"10.1016/j.crmicr.2024.100263","DOIUrl":"10.1016/j.crmicr.2024.100263","url":null,"abstract":"<div><p>The highly conserved hydrophobic pocket region of HIV-1 gp41 NHR triple-stranded coiled coil is crucial for the binding of CHR to NHR to form a six-helix bundle (6-HB). This pocket is only exposed instantaneously during fusion, making it an ideal target for antibody drug design. However, IgG molecule is too big to enter the pocket during the fusion process. Therefore, to overcome the steric hindrance and kinetic obstacles caused by the formation of gp41 pre-hairpin fusion intermediate, we obtained nanobodies (Nbs) targeting NHR by immunizing alpaca with an NHR-trimer mimic. Specifically, we identified a Nb, Nb-172, that exhibited potent and broadly neutralizing activity against HIV-1 pseudoviruses, HIV-1 primary isolates, and T20-resistant HIV-1 strains. In addition, the combinatorial use of mD1.22 and Nb-172 exhibited synergism in inhibiting HIV-1 infection inactivating cell-free virions. Nb-172 can competitively bind to the hydrophobic pocket of gp41 NHR to inhibit 6-HB formation. These findings suggest that Nb-172 merits further investigation as a potential therapeutic for HIV-1 infection.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100263"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000452/pdfft?md5=3b60eb3e8726bede2200efc33c1892e7&pid=1-s2.0-S2666517424000452-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An intracellular bacterial pathogen triggers RIG-I/MDA5-dependent necroptosis","authors":"Hang Xu ,&nbsp;Huili Li ,&nbsp;Boguang Sun ,&nbsp;Li Sun","doi":"10.1016/j.crmicr.2024.100318","DOIUrl":"10.1016/j.crmicr.2024.100318","url":null,"abstract":"<div><div>RIG-I and MDA5 are members of RIG-I-like receptors (RLRs) that detect viral RNA within the cytoplasm and subsequently initiate antiviral immune responses. Necroptosis is a form of programmed cell death (PCD) executed by mixed lineage kinase domain-like (MLKL), which, upon phosphorylation by receptor-interacting protein kinase 3 (RIPK3), causes necrotic cell death. To date, no link between RLRs and necroptosis has been observed during bacterial infection. <em>Edwardsiella tarda</em> is a zoonotic bacterial pathogen that can thrive in host macrophages. In a previous study, we identified RIG-I and MDA5 as two hub factors of RAW264.7 cells responsive to <em>E. tarda</em> infection. The present study aimed to determine the specific form of cell death triggered by <em>E. tarda</em> and explore the association between RIG-I/MDA5 and PCD in the context of bacterial infection. Our results showed that <em>E. tarda</em> infection induced RIPK3-MLKL-mediated necroptosis, rather than pyroptosis or apoptosis, in RAW264.7 cells. Meanwhile, <em>E. tarda</em> promoted RIG-I/MDA5 production and activated the RIG-I/MDA5 pathways that led to IRF3 phosphorylation, IFN-β secretion, and interferon-stimulated gene (ISG) and cytokine expression. Both RIG-I and MDA5 were essential for <em>E. tarda</em>-triggered necroptosis and required for effective inhibition of intracellular bacterial replication. Furthermore, the regulatory effect of RIG-I/MDA5 on necroptosis was not affected by type I IFN or TNF-α signaling blockage. Together these results revealed that necroptosis could be triggered by intracellular bacterial infection through the RIG-I/MDA5 pathways, and that there existed intricate interplays between PCD and RLRs induced by bacterial pathogen.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100318"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paddy seeds bacterization with ACC deaminase producing endophyte Alcaligenes faecalis SSP8 regulates physiology, leaves gas exchange parameters, PSII photochemistry and antioxidant enzymes metabolism in NaCl stressed seedlings","authors":"Shobhit Raj Vimal ,&nbsp;Jay Shankar Singh ,&nbsp;Sheo Mohan Prasad","doi":"10.1016/j.crmicr.2024.100299","DOIUrl":"10.1016/j.crmicr.2024.100299","url":null,"abstract":"<div><div>The endophytic microbes play crucial roles to crop development under stress environmental conditions. In this research, 36 endophytic bacterial strains having diverse morphology were isolated from exotic wild plant <em>Croton bonplandianus</em>. The strain SSP8 was selected for experimental study as it efficiently tolerate NaCl (0–1200 mM), produced Indole-3-acetic acid (IAA) (46 μg mL^-1) and 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase (176.70 nmol α-ketobutyrate mg^-1 protein h^-1). The SSP8 was identified as <em>Alcaligenes faecalis</em> with 16 S r<strong>-</strong>RNA gene sequencing and submitted to NCBI-USA with accession number OR225818. The <em>A. faecalis</em> SSP8 significantly enhanced paddy seeds germination percentage, seedlings vigour index and vegetative growth parameters under different NaCl (0–180 mM) regimes. The paddy seedlings chlorophyll contents, <em>Chl-a</em> fluorescence transient (PSII photochemistry), leaves gas exchange parameters were significantly enhanced in <em>A. faecalis</em> SSP8+NaCl (0–180 mM) conditions. The oxidative stress biomarkers and antioxidant enzymes activities were significantly declined in <em>A. faecalis</em> SSP8+NaCl (0–180 mM) treated seedlings. In conclusion, based on the above results the paddy seeds bacterization with <em>A. faecalis</em> SSP8 could be a bio-prospective tool to alleviate the NaCl stress and enhance the paddy crop agriculture productivity in salt affected marginal soils.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100299"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biobutanol production from underutilized substrates using Clostridium: Unlocking untapped potential for sustainable energy development","authors":"Devina Syifa Nabila ,&nbsp;Rosamond Chan ,&nbsp;Rizky Riscahya Pratama Syamsuri ,&nbsp;Puspita Nurlilasari ,&nbsp;Wan Abd Al Qadr Imad Wan-Mohtar ,&nbsp;Abdullah Bilal Ozturk ,&nbsp;Nia Rossiana ,&nbsp;Febri Doni","doi":"10.1016/j.crmicr.2024.100250","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100250","url":null,"abstract":"<div><p>The increasing demand for sustainable energy has brought biobutanol as a potential substitute for fossil fuels. The <em>Clostridium</em> genus is deemed essential for biobutanol synthesis due to its capability to utilize various substrates. However, challenges in maintaining fermentation continuity and achieving commercialization persist due to existing barriers, including butanol toxicity to <em>Clostridium</em>, low substrate utilization rates, and high production costs. Proper substrate selection significantly impacts fermentation efficiency, final product quality, and economic feasibility in <em>Clostridium</em> biobutanol production. This review examines underutilized substrates for biobutanol production by <em>Clostridium,</em> which offer opportunities for environmental sustainability and a green economy. Extensive research on <em>Clostridium</em>, focusing on strain development and genetic engineering, is essential to enhance biobutanol production. Additionally, critical suggestions for optimizing substrate selection to enhance <em>Clostridium</em> biobutanol production efficiency are also provided in this review. In the future, cost reduction and advancements in biotechnology may make biobutanol a viable alternative to fossil fuels.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100250"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000324/pdfft?md5=eaa63b3078a01f1d69255c34774c4e0c&pid=1-s2.0-S2666517424000324-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141314746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}