Current Research in Microbial Sciences最新文献

{"title":"From forest to farm: the impact of a broad spectrum of lifestyles on the porcine gut microbiota","authors":"Luke Comer ,&nbsp;Peiyang Huo ,&nbsp;Camillo Colleluori ,&nbsp;Haoran Zhao ,&nbsp;Muhammad Zeeshan Akram ,&nbsp;Romario Florent Kpossou ,&nbsp;Ester Arévalo Sureda ,&nbsp;Jan Aerts ,&nbsp;Nadia Everaert","doi":"10.1016/j.crmicr.2026.100576","DOIUrl":"10.1016/j.crmicr.2026.100576","url":null,"abstract":"<div><div>The gut microbiota is shaped profoundly by the diet, environment, and genetics of its host. Yet, previous studies have focused primarily on investigating factors individually in standardised facilities. In the present study, faecal samples and rearing metadata were collected from a highly-diverse cohort of 344 pigs, from farms to the wild, spanning eight countries and two continents. We hypothesised that varied lifestyles would induce a range of diverse microbiotas. Rustic rearing conditions were associated with increased α-diversity, alongside <em>Treponema</em>, while conventionally-reared pigs exhibited increased <em>Lactobacillus</em>. Equally, both rustic-associated bacteria and conventional feed were associated with increased short-chain fatty acids. Gas production kinetics from <em>in vitro</em> fermentation revealed the complex role of underexplored bacteria in fibre fermentation. The present study represents one of the most diverse single-study cohorts of pigs hitherto, and underscores the complex interplay between swine husbandry and the gut microbiota, and in turn, its potential consequences for functionality.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100576"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147420569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The TamA protein as a subunit vaccine improves immune protection against highly virulent Klebsiella pneumoniae infection in mice","authors":"Liang Liu ,&nbsp;Shiqi Zhang ,&nbsp;Faxian Wu,&nbsp;Linjing Zhao,&nbsp;Huajie Zhao,&nbsp;Duan Li,&nbsp;Fan Yang,&nbsp;Liang Liu","doi":"10.1016/j.crmicr.2026.100550","DOIUrl":"10.1016/j.crmicr.2026.100550","url":null,"abstract":"<div><div>Vaccination is a promising strategy for preventing <em>Klebsiella pneumoniae</em> infection, yet strain heterogeneity poses a limitation. The optimal antigen target for an anti-<em>Klebsiella pneumoniae</em> vaccine should be expressed by all or most strains. This study identified the TamA protein as highly conserved across <em>Klebsiella pneumoniae</em> strains, irrelevant of serotype. A recombinant TamA protein vaccine was developed and evaluated for protective efficacy against lethal challenge in a murine model. The recombinant TamA was cloned in <em>Escherichia coli</em> and used to immunize mice, resulting in significantly higher total serum IgG concentrations compared to adjuvant-only controls, indicating robust antibody induction. In vitro experiments showed that TamA-induced specific antibodies blocked bacterial adherence to epithelial cells. Following a lethal dose challenge, 80% of TamA-vaccinated mice survived over 10 days, compared to less than 48 h for controls. The TamA recombinant protein vaccine provided protection, with significantly reduced bacterial loads and pathological changes in vaccinated mice. This study suggests TamA as a promising candidate vaccine for preventing <em>Klebsiella pneumoniae</em> infections.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100550"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A look into the virosphere of clouds: A world yet to be explored","authors":"Janina Rahlff ,&nbsp;Pierre Amato","doi":"10.1016/j.crmicr.2025.100545","DOIUrl":"10.1016/j.crmicr.2025.100545","url":null,"abstract":"<div><div>Clouds are aqueous atmospheric systems hosting diverse and active microorganisms. Viruses may also persist despite harsh conditions, support active viral infections, and contribute to microbial dynamics during aerial transport. However, assessing viruses, and even more, virus-bacteria interactions in the atmospheric environment is highly challenging, and knowledge remains very limited. Here, based on current knowledge in cloud microbiology, we estimate the cloud virome at ∼10²¹ virus particles globally. One out of a million of cloud droplets is susceptible to host virus-bacteria interactions, which represents considerable volume at global scale, and we discuss potential implications for microbial ecology. We finally propose future research directions to explore further the cloud virosphere and its ecological roles.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100545"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic profiling of ocular surface microbiome alterations in patients with progressive supranuclear palsy-Richardson’s syndrome","authors":"Rui Zhang ,&nbsp;Yong-Kang Chen ,&nbsp;Qing-Yong Zhu ,&nbsp;Ren-Yi Feng ,&nbsp;Han Liu ,&nbsp;Ming-Ming Ma ,&nbsp;Xue-Jing Wang","doi":"10.1016/j.crmicr.2026.100605","DOIUrl":"10.1016/j.crmicr.2026.100605","url":null,"abstract":"<div><div>This study employed shotgun metagenomic sequencing to characterize the ocular surface microbiome in 20 progressive supranuclear palsy-Richardson’s syndrome (PSP-RS) patients, 17 Parkinson’s disease (PD) patients, and 30 healthy controls (HC). Comparative analysis revealed that PSP-RS patients exhibited significantly altered microbial β-diversity compared to HC, while PD patients showed no such significant changes. Both patient groups demonstrated decreased abundance of <em>g_Vibrio</em>, with PSP-RS patients additionally showing marked increases in <em>g_Acinetobacter</em> and <em>g_Anaerococcus</em>. Importantly, correlation analyses identified that increased <em>g_Acinetobacter</em> abundance was positively associated with ocular motor impairment severity, while elevated <em>g_Anaerococcus</em> levels correlated with both freezing of gait severity and longer disease duration in PSP-RS patients. This is the first shotgun metagenomic investigation of the ocular surface microbiome in PSP-RS and these findings provide evidence that specific alterations in the ocular surface microbiome may contribute to PSP-RS pathogenesis and disease progression.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100605"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147995697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and transmission processes of potentially pathogenic bacterial communities in the East Rongbuk Glaciers, Mt. Everest","authors":"Tong Ma ,&nbsp;Yeteng Xu ,&nbsp;Xinyue Wang ,&nbsp;Zhiyuan Chen ,&nbsp;Pengfei Chen ,&nbsp;Junming Guo ,&nbsp;Shichang Kang ,&nbsp;Shanjia Li ,&nbsp;Wei Zhang ,&nbsp;Tuo Chen ,&nbsp;Guangxiu Liu ,&nbsp;Binglin Zhang","doi":"10.1016/j.crmicr.2026.100553","DOIUrl":"10.1016/j.crmicr.2026.100553","url":null,"abstract":"<div><div>Glacier retreat driven by global warming is releasing previously sequestered microbial communities, including potential human pathogens, into downstream environments. However, the diversity, ecological assembly mechanisms, and dispersal processes of these bacteria remain largely unknown in high-altitude glacier systems. In this study, we characterized potential pathogenic bacterial communities along a 1200 m elevation gradient (5293–6476 m) on the East Rongbuk Glacier, northern slope of Mount Everest, using high-throughput 16S rRNA gene sequencing for snow, ice, cryoconite, and moraine samples. A total of 833 pathogenic bacterial species were identified, with Proteobacteria and Cyanobacteria dominating the communities. Distinct altitude-dependent variations were observed, with taxa such as <em>Paracoccus_yeei</em> and <em>Sphingomonas_paucimobilis</em> enriched at high elevations. Canonical correspondence analysis indicated that geographic (e.g., altitude, latitude) and environmental variables (e.g., total carbon, organic carbon) significantly influenced community structure. β-nearest taxon index (βNTI) and generalized additive models (GAMs) revealed that both deterministic and stochastic processes governed community assembly. Network analysis suggested that non-pathogenic taxa with high modularity and centrality may suppress potential pathogens through ecological interactions. Source Tracker analysis demonstrated frequent microbial exchange among glacier habitats, with moraine and cryoconite acting as dominant sources. Certain pathogenic taxa showed clear signatures of downstream migration, highlighting the potential risk of glacier-derived pathogen dissemination via meltwater and aeolian processes. This study provides a comprehensive assessment of the diversity, drivers, and dispersal of potential pathogenic bacteria in a high-altitude glacier ecosystem, offering new insights into their ecological dynamics and informing future risk assessments under climate change scenarios.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100553"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ’The lung microbiome in patients with HIV complicated with community-acquired pneumonia: across-sectional pilot study’ Current Research in Microbial Sciences Volume 9, 2025, 100480","authors":"Yang Song ,&nbsp;Xinmin Xu ,&nbsp;Mengjiao Xie ,&nbsp;Jing Tao ,&nbsp;Huiling Jin ,&nbsp;Yang Liu ,&nbsp;Li Liu ,&nbsp;Xiaohui Song ,&nbsp;Shuang Meng ,&nbsp;Io Hong Cheong ,&nbsp;Yajie Wang ,&nbsp;Qiang Wei","doi":"10.1016/j.crmicr.2025.100537","DOIUrl":"10.1016/j.crmicr.2025.100537","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100537"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mangrove cyanobacterial diversity as a source of bioactive natural products","authors":"Arup Ratan Roy ,&nbsp;Shayontani Basu ,&nbsp;Sergio de los Santos Villalobos ,&nbsp;Joydeep Mukherjee","doi":"10.1016/j.crmicr.2026.100557","DOIUrl":"10.1016/j.crmicr.2026.100557","url":null,"abstract":"<div><div>Extensive exploration of lesser-explored habitats undertaken in search of novel and sustainable sources of pharmaceuticals, agrochemicals, renewable energy sources, and dyestuffs has uncovered the capability of cyanobacteria or blue-green algae as producers of these commodities. Mangroves represent one such highly abiotic-stressed and vulnerable biodiversity hotspot found in the intertidal regions of the tropics and sub-tropics that harbor diverse microflora. However, limited data on cyanobacterial taxonomic classification and the study of secondary metabolites rich in novel molecules are currently available, as compared to other prokaryotes. The discovery of useful natural products from cyanobacteria is limited since few strains can be genetically modified, and beneficial compounds are often produced in low or inconsistent amounts. Difficulties with transferring biosynthetic pathways to other hosts, combined with the high cost and ecological challenges of scaling cultivation and advanced screening, require integrated multiomics and simple, scalable workflows for industrial translation. This article attempts to review the reported bioactive secondary metabolites of importance produced by cyanobacteria from mangrove ecosystems across the world and the modern methods that can be utilized for bioactive compound discovery from these cyanobacteria.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100557"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial for special issue “Microorganisms and food security under climate change scenarios: From taxonomy to host–microbe interactions”","authors":"Brenda Román-Ponce ,&nbsp;Fernando Uriel Rojas-Rojas","doi":"10.1016/j.crmicr.2026.100601","DOIUrl":"10.1016/j.crmicr.2026.100601","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100601"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148241540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic engineering of gut commensals: heterologous protein expression in Bifidobacterium animalis subsp. lactis CECT8145","authors":"Alba Arévalo-Lalanne ,&nbsp;Paola Corbín-Agustí ,&nbsp;Daniel Ramón ,&nbsp;Javier Buceta ,&nbsp;Juli Peretó ,&nbsp;Marta Tortajada","doi":"10.1016/j.crmicr.2026.100591","DOIUrl":"10.1016/j.crmicr.2026.100591","url":null,"abstract":"<div><div><em>Bifidobacterium</em> is a key genus of the gut microbiota. Among commercially used probiotics, <em>Bifidobacterium animalis</em> subsp. <em>lactis</em> CECT 8145 has been shown to exert health benefits in metabolic health and healthy aging. Remarkably, these effects persist in its postbiotic, heat-treated form, a feature attributed to its unique lipoteichoic acid composition. However, the challenges of genetically manipulating bifidobacteria hinder the identification of the molecular basis of this functionality. In this study, we report the first successful transformation of <em>B. animalis</em> subsp. <em>lactis</em> CECT 8145, alongside reproducible and high overexpression of heterologous recombinant proteins. Our approach integrates optimized electroporation protocols, tailored vector selection, and modulation of membrane fluidity, further enhanced by polyethylene glycol as a permeabilizing agent. Membrane fluidity - driven by the ratio of unsaturated to saturated fatty acids - is shown to be a quantifiable and tunable determinant of transformation efficiency. A comparative analysis of five <em>B. animalis</em> strains confirmed that transformation efficiency remains strongly strain-dependent, likely reflecting differences in ecological origin. Promoter pHUP yielded the highest heterologous protein expression levels, showing that careful promoter selection can substantially increase gene expression even in strains with low transformability, with protein expression levels increasing with plasmid copy number and reaching up to 10⁴ arbitrary fluorescence units. Together, these findings establish a practical framework for the genetic engineering of recalcitrant, commensal probiotic strains such as CECT 8145. This work supports the development of next-generation probiotics and enables molecular validation of their health-promoting effects.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100591"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147656364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish gut-water interface as a hotspot for the dissemination of antibiotic resistance genes across natural and aquaculture systems","authors":"Padma Dolkar,&nbsp;L. Themchuirin,&nbsp;Nongmaithem Sonia,&nbsp;Apoorva Atri,&nbsp;Pankaj Yadav,&nbsp;Sneha Siwach,&nbsp;Sonakshi Modeel,&nbsp;Ram Krishan Negi","doi":"10.1016/j.crmicr.2026.100646","DOIUrl":"10.1016/j.crmicr.2026.100646","url":null,"abstract":"<div><div>Freshwater ecosystems are important reservoirs and transmission pathways for antibiotic resistance genes (ARGs), yet host-mediated microbial selection and anthropogenic pressure on fish gut resistome remain poorly characterised, especially in major South Asian river systems. We performed shotgun metagenomics and genome-resolved binning from 194 fish representing four species, along with host-associated water samples, collected from six geographically distinct sites spanning two major river systems (the Yamuna and the Indus) and two aquaculture farms. The fish gut nurtures distinct microbial communities from the surrounding water, revealing strong host-mediated filtering of environmental microbiota. Across all samples, 1108 ARG subtypes conferring resistance to 14 antibiotic classes were detected, including extended-spectrum β-lactamases (<em>blaTEM and blaCTX-M</em>) and WHO critical-priority carbapenemases (<em>blaIMP and blaOXA</em>). Fish from the Indus River maintained diverse but comparatively stable resistomes dominated by intrinsic chromosomal efflux mechanisms, whereas fish from the urbanized Yamuna River, particularly <em>Labeo boggut</em>, exhibited noticeable enrichment of clinically important ARGs. The prevalence of mobile genetic elements (MGEs) and virulence factors (VFs) were consistently more abundant in the fish gut microbiome than in the host’s surrounding water, indicating an increased potential for horizontal gene transfer and microbial persistence. A total of 19 metagenome-assembled genomes (MAGs) carrying multiple ARGs, VFs, and plasmid-associated markers were detected, identifying bacterial populations capable of maintaining and disseminating antimicrobial resistance. These outcomes confirm that fish inhabiting anthropogenically influenced river systems can serve as important reservoirs of clinically relevant resistance determinants, highlighting potential risks for environmental dissemination, aquaculture and human exposure through aquatic ecosystems.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100646"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148642126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}