Current Research in Microbial Sciences最新文献

{"title":"Microbial dark matter spearheading the biogeochemical cycle in the Solar Lake of Taba, Egypt","authors":"Rehab Z. Abdallah ,&nbsp;Ali H.A. Elbehery ,&nbsp;Amged Ouf ,&nbsp;Rania Siam","doi":"10.1016/j.crmicr.2025.100433","DOIUrl":"10.1016/j.crmicr.2025.100433","url":null,"abstract":"<div><div>Microbial dark matter (MDM) represents a vast, uncultured fraction of microbial life with largely unknown ecological roles, particularly in extreme environments. This study investigates MDM in the hypersaline microbial mats of Solar Lake, Taba, Egypt, using shotgun metagenomics (∼70 M reads/sample; triplicate sampling across four sites). A total of 364 metagenome-assembled genomes (MAGs) were recovered, of which 116 (∼30 %) were classified as MDM, comprising 55 % archaeal and 45 % bacterial lineages. Functional annotation revealed that ∼14 % of the MDM MAGs had the genetic potential to fix carbon. The genetic makeup of <em>Ca</em>. Lokiarchaeota (5 MAGs, 4.3 %) and Heimdallarchaeota (1 MAG, 0.86 %) suggest a mixotrophic lifestyle. Some non-methanogenic MAGs had the genetic capacity to utilize methanol and glycine-betaine as carbon sources. In addition, <em>Ca</em>. Marinisomatota MAGs had the genetic potential to degrade polysaccharides, while KSB1 MAGs harbored genes for carbohydrate degradation, denitrification, and nitrogen fixation. The high relative abundance of the SOX gene complex, in <em>Ca</em>. Asgardarchaeota and <em>Ca</em>. Coatesbacteria (RBG-13–66–14), highlights MDM community involvment in thiosulfate oxidation. Additionally, a novel Myxococcota MAG encoded a complete photosynthetic gene cluster, including photosystem II, suggesting phototrophic activity along with Cyanobacteria. Collectively, the genetic makeup of the Solar Lake MDM community underpins key processes such as carbon cycling, sulfur reduction, thiosulfate oxidation, nitrogen fixation, and denitrification, driving the biogeochemical dynamics of this unique hypersaline ecosystem.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100433"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial metabolites from a heterobranchia-associated bacteria and their prey from Bali, Indonesia","authors":"Rhesi Kristiana ,&nbsp;Ni Kadek Dita Cahyani ,&nbsp;Yang Jin ,&nbsp;I Wayan Mudianta ,&nbsp;Fadillaisyia Riandani Putri ,&nbsp;Kharisma Ayu Zeina Halisah ,&nbsp;Meng-Xue Wang ,&nbsp;Yue-Wei Guo ,&nbsp;Xu-Wen Li ,&nbsp;Ocky Karna Radjasa","doi":"10.1016/j.crmicr.2025.100448","DOIUrl":"10.1016/j.crmicr.2025.100448","url":null,"abstract":"<div><div>Marine-associated bacteria serve as a significant source of bioactive natural products for drug discovery efforts. The microbial symbionts of heterobranchs and their prey represent a promising source of bioactive compounds with potential applications as pharmaceutical agents. This study aimed to evaluate the diversity of associated microbes of heterobranchs and their prey that were obtained from one of marine biodiversity hotspots. The next step involved employing in vitro antibacterial screening of the organic extracts of the bacterial strains to determine the most potent ones. Microbiome study revealed that <em>Hypselodoris roo</em> had the most diverse microbiome, with Proteobacteria and Firmicutes bacterial taxa predominating among six heterobranchia samples. A total of 46 bacterial strains and 243 actinobacterial strains have been isolated from six nudibranchs and their prey sources. Next, <em>Ruegeria</em> sp., a symbiont from the sponge prey, exhibited the most promising antibacterial. Subsequent chromatographic purification revealed the presence of three known cyclic dipeptides: cyclo(Leu-Pro) (<strong>1</strong>), cyclo(Phe-Pro) (<strong>2</strong>), and cyclo(phenylalanine-4‑hydroxy-proline) (<strong>3</strong>), along with an indazole <strong>4</strong> in the extract of <em>Ruegeria</em> sp. All the metabolites have never been reported from this symbiont bacteria associated to the organisms examined in this study.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100448"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel secondary metabolite from a new species of Hypoxylon saxatilis sp. nov. for suppressing bacterial wilt in tomato","authors":"Thanapat Suebrasri ,&nbsp;Wasan Seemakram ,&nbsp;Awat Wisetsai ,&nbsp;Thanawan Gateta ,&nbsp;Sutarin Preepram ,&nbsp;Phornnapa Saentao ,&nbsp;Sophon Boonlue","doi":"10.1016/j.crmicr.2025.100445","DOIUrl":"10.1016/j.crmicr.2025.100445","url":null,"abstract":"<div><div>The bacterium <em>Ralstonia solanacearum</em> is an important pathogen that causes wilt disease in tomatoes, leading to a reduction in tomato yield. Therefore, this study aimed to investigate the antibacterial activity of endophytic fungi isolated from medicinal plants against the wilt-causing bacterium <em>R. solanacearum</em> in tomatoes under both laboratory and greenhouse conditions. Forty-eight fungi were isolated from 20 medicinal plant species using the surface sterilization technique. The antagonistic activity against <em>R. solanacearum</em> was subsequently examined using the agar plug diffusion test. Two isolates, <em>Hypoxylon</em> sp. NGPM and <em>Hypoxylon saxatilis</em> KKU-KHP 01, were identified from the midrib of the <em>Acanthus ebracteatus</em> and the petiole of the <em>Gardenia saxatilis</em> (Geddes), respectively. Furthermore, the biologically active compounds extracted from both fungal endophytes inhibited the pathogen in the fungal fermentation broth. Notably, <em>H. saxatilis</em> KKU-KHP 01 is a newly identified fungal species and the first within its genus to produce tetrahydrofuran, a novel compound not previously reported as a natural secondary metabolite. This compound exhibits inhibitory activity against the phytopathogen <em>R. solanacearum</em>. Scanning electron microscopy revealed that exposure to pure tetrahydrofuran caused structural damage to the bacterial cell wall, resulting in visibly wrinkled cells. Additionally, under greenhouse conditions, the crude extract of <em>H. saxatilis</em> KKU-KHP 01 could reduce the severity of wilt disease by up to 83.33%, effectively caused by tetrahydrofuran. Therefore, tetrahydrofuran can be applied to control wilt disease in tomatoes under field conditions and can be further developed as a commercial biocontrol agent.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100445"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved loop mediated isothermal amplification based assay for the rapid identification of genomic DNA of Burkholderia pseudomallei","authors":"Saika Farook ,&nbsp;Arittra Bhattacharjee ,&nbsp;Farhan Muhib Udoy ,&nbsp;Robiul Islam Rubel ,&nbsp;Fahmida Rahman ,&nbsp;Lovely Barai ,&nbsp;K.M. Shahidul Islam ,&nbsp;Jalaluddin Ashraful Haq ,&nbsp;Md. Shariful Alam Jilani","doi":"10.1016/j.crmicr.2025.100463","DOIUrl":"10.1016/j.crmicr.2025.100463","url":null,"abstract":"<div><div><em>Burkholderia pseudomallei</em>, the causative agent of the potentially life-threatening infectious disease, melioidosis, is endemic in Bangladesh. The spatial clustering of clinical incidence and the fatal outcome of melioidosis necessitates unequivocal and rapid detection and identification. Culture constitutes the gold standard test for the diagnosis of melioidosis, but identification of <em>B. pseudomallei</em> by culture is time consuming. In the view of the above, the present study has been designed to develop a Loop Mediated Isothermal Amplification based assay (LAMP) for the rapid and accurate detection of <em>B. pseudomallei</em>.</div><div>Twenty-two culture confirmed <em>B. pseudomallei</em> isolates and 18 non <em>B. pseudomallei</em> bacterial species were included in the study. DNA were extracted and amplified by in-house LAMP assay, utilizing a set of newly designed primers at optimized conditions. The LAMP product was visualized by various methods. The limit of detection (LOD) of LAMP was determined and detection of <em>B. pseudomallei</em> directly from sterile pre-inoculated urine by the LAMP technique was evaluated.</div><div>All 22 <em>B pseudomallei</em> were positive by the in-house LAMP while the 18 heterogeneous bacterial species were LAMP negative. The target sequence amplified by the LAMP outer primers showed the highest similarity with <em>B. pseudomallei</em> isolate UKMH10, confirming the specificity of the newly designed primers. The LOD of the in-house LAMP assay was found to be 0.78 pg, which is much lower than the LOD of PCR (0.78 ng). The in-house LAMP was also found positive for <em>B. pseudomallei</em> using direct sample such as pus and pre-inoculated urine. A closed tube LAMP assay using agar dye capsule was adopted to minimize contamination with LAMP amplification product and thus to increase the overall precision of the test.</div><div>Rapid and accurate detection of <em>B. pseudomallei</em> was developed using LAMP-based assay with a set of in-house designed primers. This detection procedure can be established for a cost-effective diagnosis of melioidosis at the rural level.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100463"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiome species Levilactobacillus brevis regulates reproductive fitness in C. elegans","authors":"Nicole J. Braun,&nbsp;Danielle E. Mor","doi":"10.1016/j.crmicr.2025.100471","DOIUrl":"10.1016/j.crmicr.2025.100471","url":null,"abstract":"<div><div>The human gut microbiome has attracted considerable attention in recent years due to its broad impact on various physiological processes; however, the mechanisms underlying host-microbiota interactions are not fully understood. In particular, direct causal relationships between specific species and host health outcomes remain to be established. Here we examined the effects of human gut microbiome species <em>Levilactobacillus brevis</em> (<em>L. brevis</em>) on host health using <em>C. elegans</em> and found that <em>L. brevis</em> feeding caused significant reproductive dysfunction, including severe egg retention leading to fewer eggs laid, and abnormal egg morphologies. These effects were associated with impaired serotonin signaling in hermaphrodite-specific neurons (HSNs), which regulate egg-laying, while vulval muscle function remained intact. Progeny from <em>L. brevis</em>-fed worms exhibited reduced viability and delayed development, suggesting an overall reduction in <em>C. elegans</em> reproductive fitness. Our findings align with emerging evidence linking gut dysbiosis to reproductive health disorders in humans, and underscore the need to explore specific roles of individual gut microbiota in host health and physiology. Our work also highlights the utility of <em>C. elegans</em> as a model for studying the complex interactions between the microbiome and the host.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100471"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and expression of the dimethylsulfoniopropionate lyase gene dddY and the identification of the key amino acids necessary for its activity","authors":"Yongchuang Liu ,&nbsp;Feilong Ma ,&nbsp;Hongfei Zhang ,&nbsp;Cuiwei Chu ,&nbsp;Xiaohui Wang ,&nbsp;Yuehui Tang ,&nbsp;Jian He ,&nbsp;Jiguo Qiu ,&nbsp;Siqiong Xu ,&nbsp;Lili Li","doi":"10.1016/j.crmicr.2025.100444","DOIUrl":"10.1016/j.crmicr.2025.100444","url":null,"abstract":"<div><div>The abundant solute dimethylsulfoniopropionate (DMSP) is crucial in marine ecosystems. In this study, a bacterium, <em>Acinetobacter</em> sp. ZS25, capable of completely mineralizing DMSP and producing DMS and acrylate, was isolated. The possible DMSP degradation pathway was primarily identified. The role of DMSP lyases DddY was identified through a combination of genomic comparison, gene knockout and heterologous expression. The <em>K</em>_m and <em>k</em>_cat of AsDddY for DMSP were 2.6 mM and 12.7 × 10³ s⁻¹, respectively. Site-directed mutagenesis was employed to examine the influence of specific amino acid residues (Thr131, Asp181, Tyr225, Gly230, Gly250, His263, His265, Glu269, Tyr271, Leu274, Tyr331, and His338) within AsDddY, elucidating their critical roles in the protein's functionality. Bioinformatics analysis revealed 19 distinct <em>acu-dddY</em> cluster order types, while the number of strains with a complete <em>dddY</em>-<em>acu</em> cluster is limited. Our findings offer novel insights into the mechanisms underlying DMSP biodegradation and enhance our understanding of the diversity of <em>acu</em>-<em>dddY</em> clusters present in natural bacterial populations.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100444"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil microbial resources: Unlocking sustainable strategies for crop productivity and soil health","authors":"Sandeep Sharma ,&nbsp;Nihar Gupta ,&nbsp;Sukhjinder Kaur ,&nbsp;Kailash Chand Kumawat ,&nbsp;Anmoldeep Singh Chakkal","doi":"10.1016/j.crmicr.2025.100468","DOIUrl":"10.1016/j.crmicr.2025.100468","url":null,"abstract":"<div><div>Using efficient rhizospheric microbes as bio-inoculants is a key factor in promoting agricultural sustainability, as these microbes have been shown to enhance plant growth promotion and crop productivity. Soil microbial communities offer numerous benefits to crops, including improved nutrient uptake efficiency, phytohormone production, improved soil structure, nutrient balance, enhanced plant and soil health, protection against soil borne phyto-pathogens and increased plant resilience to abiotic and biotic stresses. These characteristics of rhizospheric microbes have attracted researcher's attention, as using these microbes individually or in consortia has enhanced soil fertility as well as soil heatlh in a eco-friendly manner. Further, the utilization of multi-omics techniques for exploring the hidden potential of beneficial plant growth promoting microbes is one of the novel approaches that can result in the generation of new biological formulations under changing climatic conditions. This review is therefore focused on the diversity of microbes, their contributions for plant growth promotion and yield with advances in techniques for screening and development of efficient bio-inoculant with a special emphasis on promoting sustainable agriculture practices.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100468"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional genomic analysis of Bacillus cereus BC4 strain for chromium remediation in contaminated soil","authors":"Zhiyi Liu ,&nbsp;Yubing Cai ,&nbsp;Xu Chen ,&nbsp;Yan Cang ,&nbsp;Jialiang Yu ,&nbsp;Muhammad Shaaban ,&nbsp;Yajun Cai ,&nbsp;Qi-an Peng","doi":"10.1016/j.crmicr.2025.100388","DOIUrl":"10.1016/j.crmicr.2025.100388","url":null,"abstract":"<div><div>Soil provides a habitat for microorganisms that can mitigate metal contamination. This study presents <em>Bacillus cereus</em> BC4 strain, which shows significant potential for metal pollution remediation. This bacterium achieved a 98.6 % reduction in Chromium (VI) concentrations from 300 mg/L to negligible levels under specific conditions (pH 8, 37 °C, and 120 rpm agitation) in LB medium. The complete genome of <em>Bacillus cereus</em> BC4 was sequenced using Oxford Nanopore Technology, revealing a circular chromosome and a plasmid with a total of 5537,675 base pairs and a <em>G</em> + <em>C</em> content of 35.44 %. Fourteen genes critical for Cr metabolism were identified. qRT-PCR demonstrated that under low Cr(VI) stress, two genes, <em>chrA</em> and <em>nitR1</em>, were up-regulated, indicating their role in Cr resistance. The genome revealed gene clusters essential for resilience against various metals, including chromium, arsenic, copper, manganese, and cadmium, as well as for synthesizing secondary metabolites crucial for survival and adaptation. Additionally, genes associated with biopolymer synthesis were identified, emphasizing the organism's diverse genetic capabilities. This genomic study led to the submission of the complete genome to GenBank (CP101135), enhancing the understanding and potential of <em>Bacillus cereus</em> BC4 in chromium remediation and environmental restoration.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100388"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potency of all-D amino acid antimicrobial peptides derived from the bovine rumen microbiome on tuberculous and non-tuberculous mycobacteria","authors":"Céline Boidin-Wichlacz ,&nbsp;Marc Maresca ,&nbsp;Isabelle Correia ,&nbsp;Olivier Lequin ,&nbsp;Vanessa Point ,&nbsp;Magali Casanova ,&nbsp;Alexis Reinbold ,&nbsp;Olga Iranzo ,&nbsp;Sharon A. Huws ,&nbsp;Priscille Brodin ,&nbsp;Linda B. Oyama ,&nbsp;Aurélie Tasiemski ,&nbsp;Stéphane Canaan ,&nbsp;Jean-François Cavalier","doi":"10.1016/j.crmicr.2025.100395","DOIUrl":"10.1016/j.crmicr.2025.100395","url":null,"abstract":"<div><div>Despite the availability of antibiotics, tuberculosis (TB), caused by <em>Mycobacterium tuberculosis</em>, was once again declared the world's leading cause of death from a single infectious agent in 2023. Furthermore, the rising prevalence of drug-resistant strains of <em>M. tuberculosis</em>, coupled with the limitations of existing therapeutics, underscores the urgent need for new antimicrobial agents that act through different mechanisms, thereby providing novel therapeutic options. From this perspective, antimicrobial peptides (AMPs) derived from the bovine rumen microbiome have shown promise against many resistant pathogens and may therefore offer a promising alternative against TB. Here, we evaluated the efficacy of AMPs from bovine rumen microbiome, namely the <strong>Lynronne 1, 2</strong> &amp; <strong>3</strong> and <strong>P15s</strong> as well as their <strong>all-D</strong> amino acid enantiomers, against non-tuberculous (<em>M. abscessus, M. marinum</em> and <em>M. smegmatis)</em> and tuberculous (<em>M. bovis</em> BCG, <em>M. tuberculosis</em>) mycobacteria. In particular, their antimycobacterial activity was assessed against extracellularly and intracellularly replicating <em>M. tuberculosis</em> H37Rv pathogenic strain. Their innocuity was further studied by determining their respective cytotoxicity against human cell lines and hemolytic activity on human erythrocytes. Finally, their mechanism of action was investigated by a membrane permeabilization assay and a lipid insertion assay <em>via</em> surface pressure measurement. Although <strong>all-D</strong> enantiomers showed increased cytotoxicity to human cell lines, they still offer a good therapeutic window with improved activity compared to their L-form counterparts, especially <strong>Lynronne 2D<em>_all</em></strong> and <strong>P15sD<em>_all</em></strong> which emerged as the best growth inhibitors of all mycobacteria. Remarkably, the <strong>all-D</strong> enantiomers also demonstrated activity against intramacrophagic replicating <em>M. tuberculosis</em> H37Rv, with very limited toxicity towards human cells and no hemolytic activity at their respective minimum inhibitory concentration. Membrane permeabilization and monolayer lipid insertion assays suggested that these peptides mostly act by insertion into the mycobacterial membrane resulting in a rapid membranolytic effect. These findings highlight the potential of the <strong>all-D</strong> enantiomers of Lynronne peptides, as attractive candidates for the development of new anti-TB drugs. Their effective antibacterial properties combined with low toxicity underscore <strong>Lynronne 2D<em>_all</em></strong> and <strong>P15sD<em>_all</em></strong> as building blocks for the development of promising alternatives to conventional antibiotics in the treatment of mycobacterial infections, particularly against <em>M. tuberculosis</em>.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100395"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity and impact on keratinocyte cell growth of Cutibacterium acnes bacteriophages in a Cutibacterium acnes IA1- colonized keratinocyte model","authors":"Juan C Farfán-Esquivel ,&nbsp;María Victoria Gutiérrez ,&nbsp;Alejandro Ondo-Méndez ,&nbsp;John M González ,&nbsp;Martha J Vives-Flórez","doi":"10.1016/j.crmicr.2025.100356","DOIUrl":"10.1016/j.crmicr.2025.100356","url":null,"abstract":"<div><div>Acne is an inflammatory disease in which microbial disbalance is represented by an augmented population of phylotype IA₁ of <em>Cutibacterium acnes</em>. Various treatments for acne can cause side effects, and it has been reported that <em>C. acnes</em> is resistant to prescribed antibiotics. Phage therapy has been proposed as an alternative treatment for acne, given its species-specificity to kill bacteria, its relative innocuity, and its potential to manage antibiotic-resistant pathogens. Moreover, bacteriophages (phages) may modulate the microbiota and immune responses. Some studies have shown the potential use of phages in the treatment of acne. Nevertheless, the capacity to specifically reduce phylotype IA₁ and the effect of phage treatment on skin cells are poorly understood. We assessed the capacity of phages to clear <em>C. acnes</em> IA₁ and their effects on cell cytotoxicity and growth in HEKa cells<em>- C. acnes</em> IA₁ co-culture. Phylotypes IA₁ and IB had similar effects on HEKa cells, causing cytotoxicity and diminishing cell growth. Nevertheless, IA₁ caused a higher impact on cell doubling time by increasing it 1.8 times more than cell growth control group. Even though there are no phages IA₁-specific, we found phages that have a diminished effect on other phylotypes not related to acne. Phage treatment in general reduced IA₁-caused cytotoxicity, with differences in efficacy among phages. In addition, phage purification was necessary to restore metabolic activity and growth of HEKa. Overall, phage evaluation as a therapeutic alternative should include phage-bacteria interactions and their impact on skin cells because of the differences that each phage can exhibit.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100356"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}