Deciphering the complex interplay between gut microbiota and crop residue breakdown in forager and hive bees (Apis mellifera L.)

IF 4.8 Q1 MICROBIOLOGY
M.N. Rudra Gouda , K.M. Kumaranag , B. Ramakrishnan , Sabtharishi Subramanian
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引用次数: 0

Abstract

This study investigates A. mellifera gut microbiota diversity and enzymatic activities, aiming to utilize identified isolates for practical applications in sustainable crop residue management and soil health enhancement. This study sampled honey bees, analyzed gut bacterial diversity via 16S rRNA gene, and screened isolates for cellulolytic, hemicellulolytic, and pectinolytic activities, with subsequent assessment of enzymatic potential. The study reveals that cellulolytic and hemicellulolytic bacterial isolates, mainly from γ-Proteobacteria, Actinobacteria, and Firmicutes, have significant potential for crop residue management. Some genera, like Aneurinibacillus, Bacillus, Clostridium, Enterobacter, Serratia, Stenotrophomonas, Apilactobacillus, Lysinibacillus, and Pseudomonas, are very good at breaking down cellulose and hemicellulase. Notable cellulose-degrading genera include Cedecea (1.390 ± 0.57), Clostridium (1.360 ± 0.86 U/mg), Enterobacter (1.493 ± 1.10 U/mg), Klebsiella (1.380 ± 2.03 U/mg), and Serratia (1.402 ± 0.31 U/mg), while Aneurinibacillus (1.213 ± 1.12 U/mg), Bacillus (3.119 ± 0.55 U/mg), Enterobacter (1.042 ± 0.14 U/mg), Serratia (1.589 ± 0.05 U/mg), and Xanthomonas (1.156 ± 0.08 U/mg) excel in hemicellulase activity. Specific isolates with high cellulolytic and hemicellulolytic activities are identified, highlighting their potential for crop residue management. The research explores gut bacterial compartmentalization in A. mellifera, emphasising gut physiology's role in cellulose and hemicellulose digestion. Pectinolytic activity is observed, particularly in the Bacillaceae clade (3.229 ± 0.02), contributing to understanding the honey bee gut microbiome. The findings offer insights into microbiome diversity and enzymatic capabilities, with implications for biotechnological applications in sustainable crop residue management. The study concludes by emphasizing the need for ongoing research to uncover underlying mechanisms and ecological factors influencing gut microbiota, impacting honey bee health, colony dynamics, and advancements in crop residue management.

Abstract Image

解读觅食蜂和蜂巢蜂肠道微生物群与作物残留物分解之间复杂的相互作用
本研究调查了蜜蜂肠道微生物群的多样性和酶活性,旨在将鉴定出的分离物实际应用于可持续作物残留管理和土壤健康改善。本研究对蜜蜂进行了采样,通过 16S rRNA 基因分析了肠道细菌的多样性,并对分离物的纤维素分解、半纤维素分解和果胶分解活性进行了筛选,随后对酶的潜力进行了评估。研究发现,纤维素分解菌和半纤维素分解菌分离物主要来自γ-蛋白细菌、放线菌和真菌,在作物残留物管理方面具有巨大潜力。一些菌属,如Aneurinibacillus、Bacillus、Clostridium、Enterobacter、Serratia、Stenotrophomonas、Apilactobacillus、Lysinibacillus 和 Pseudomonas,在分解纤维素和半纤维素酶方面非常出色。值得注意的纤维素降解菌属包括 Cedecea(1.390 ± 0.57)、Clostridium(1.360 ± 0.86 U/mg)、Enterobacter(1.493 ± 1.10 U/mg)、Klebsiella(1.380 ± 2.03 U/mg)和 Serratia(1.402 ± 0.31 U/mg),而Aneurinibacillus(1.213 ± 1.12 U/mg)、Bacillus(3.119 ± 0.55 U/mg)、Enterobacter(1.042 ± 0.14 U/mg)、Serratia(1.589 ± 0.05 U/mg)和Xanthomonas(1.156 ± 0.08 U/mg)在半纤维素酶活性方面表现突出。研究发现了具有高纤维素分解和半纤维素分解活性的特定分离菌株,凸显了它们在作物残留物管理方面的潜力。该研究探索了 A. mellifera 的肠道细菌分区,强调了肠道生理在纤维素和半纤维素消化中的作用。观察到果胶溶解活性,特别是在芽孢杆菌科支系中(3.229 ± 0.02),有助于了解蜜蜂肠道微生物组。这些发现有助于深入了解微生物组的多样性和酶解能力,对生物技术在可持续作物残留物管理中的应用具有重要意义。研究最后强调,需要持续开展研究,以发现影响肠道微生物群的潜在机制和生态因素,从而影响蜜蜂健康、蜂群动态和作物残留物管理的进步。
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来源期刊
Current Research in Microbial Sciences
Current Research in Microbial Sciences Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
7.90
自引率
0.00%
发文量
81
审稿时长
66 days
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