iScience最新文献_第5页

Aromatics from fossil fuels and breast cancer

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-10 DOI: 10.1016/j.isci.2025.112204

Leena Hilakivi-Clarke , Theresa Krista Jolejole , Joas Lucas da Silva , Fabia de Oliveira Andrade , Gail Dennison , Steffen Mueller

{"title":"Aromatics from fossil fuels and breast cancer","authors":"Leena Hilakivi-Clarke , Theresa Krista Jolejole , Joas Lucas da Silva , Fabia de Oliveira Andrade , Gail Dennison , Steffen Mueller","doi":"10.1016/j.isci.2025.112204","DOIUrl":"10.1016/j.isci.2025.112204","url":null,"abstract":"<div><div>Polycyclic aromatic hydrocarbons (PAHs) from fossil fuels initiate breast cancer in animal models, and in humans a link between PAH exposure and breast cancer risk has been established. In women, it takes approximately two decades for PAH-exposed breast cells to progress to diagnosable breast cancer, and the exposure needs to happen during a time window when breast is vulnerable to PAHs. Further, not everyone exposed to high levels of PAHs develops breast cancer. PAHs are most likely to lead to breast cancer initiation among individuals who were exposed <em>in utero</em> through pregnant mothers to environmental pollutants or maternal obesity or both. These early life exposures are shown to increase daughter’s later susceptibility to breast cancer by causing in the daughter (1) an increase in the number of structures in the breast in which breast cancer initiation takes place, (2) a suppression, perhaps epigenetically, in the ability of cells to repair DNA damage caused by PAHs by inhibiting expression of tumor suppressor genes, or (3) a persistent gut dysbiosis, which then impacts immune cells in the tumor microenvironment. Among the early life environmental pollutants that increase breast cancer susceptibility may be volatile aromatic BTEX compounds. Thus, aromatics from fossil fuels are likely to be involved in causing breast cancer, and efforts should be directed toward reducing human exposure to these compounds to prevent breast cancer.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112204"},"PeriodicalIF":4.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theranostic advances and the role of molecular imprinting in disease management

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-10 DOI: 10.1016/j.isci.2025.112186

Eylul Gulsen Yilmaz , Beyza Nur Küçük , Yusuf Aslan , Özgecan Erdem , Yeşeren Saylan , Fatih Inci , Adil Denizli

{"title":"Theranostic advances and the role of molecular imprinting in disease management","authors":"Eylul Gulsen Yilmaz , Beyza Nur Küçük , Yusuf Aslan , Özgecan Erdem , Yeşeren Saylan , Fatih Inci , Adil Denizli","doi":"10.1016/j.isci.2025.112186","DOIUrl":"10.1016/j.isci.2025.112186","url":null,"abstract":"<div><div>Molecular imprinting has become an effective technology in the realm of diagnosing diseases, providing unparalleled specificity and sensitivity. This method is a promising trend in current medical research. This review examines the utilization of molecularly imprinted polymers (MIPs) in theranostic that integrates diagnostic functionalities for personalized medicine. The present work briefly discusses the fundamental concepts of molecular imprinting and how it has evolved into a versatile platform. Subsequently, the utilization of MIPs in the advancement of biosensors is focused, specifically emphasizing their contribution to the detection and diagnosis of diseases. The therapeutic potential of MIPs, focusing on targeted drug delivery and controlled release systems and the integration of MIPs into theranostic platforms is explored through case studies, showcasing the technology’s ability to simultaneously diagnose and treat diseases. Finally, we address the current challenges facing MIPs and discuss future perspectives, emphasizing the potential of this technology to revolutionize the next generation.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112186"},"PeriodicalIF":4.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diffusion mechanism and adsorbed-phase classification—molecular simulation insights from Lennard-Jones fluid on MOFs

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-08 DOI: 10.1016/j.isci.2025.112181

Haonan Chen , Sagar Saren , Xuetao Liu , Ji Hwan Jeong , Takahiko Miyazaki , Young-Deuk Kim , Kyaw Thu

{"title":"Diffusion mechanism and adsorbed-phase classification—molecular simulation insights from Lennard-Jones fluid on MOFs","authors":"Haonan Chen , Sagar Saren , Xuetao Liu , Ji Hwan Jeong , Takahiko Miyazaki , Young-Deuk Kim , Kyaw Thu","doi":"10.1016/j.isci.2025.112181","DOIUrl":"10.1016/j.isci.2025.112181","url":null,"abstract":"<div><div>Physisorption of gases has been widely applied in thermal energy utilization and purification processes. Diffusion in porous media has been well studied. However, molecular-scale adsorbate diffusion mechanism remains unexplored. In this study, molecular dynamics have been employed to elucidate the diffusion behaviors of liquid and gaseous methane adsorbed in Cu-BTC (Copper(2+) 1,3,5-benzenetricarboxylate). Based on the energy distribution and trajectories of adsorbed molecules, a hypothesis is proposed that the adsorbed phase can be classified into four types: bound molecules (oscillate around a specific region of the adsorbent), generally adsorbed molecules (within the range of surface interaction and possess negative total energy), non-adsorbed molecules (within the range of surface interaction, but having positive total energy), and free molecules (beyond the range of surface interaction). To support this hypothesis, further simulation of methane adsorption in MOF-5 (Zn<sub>4</sub>O(BDC)<sub>3</sub>) has been conducted and compared with existing experimental data, indicating the hypothesis has broader applicability.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112181"},"PeriodicalIF":4.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disuse plasticity limits spinal cord injury recovery

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-08 DOI: 10.1016/j.isci.2025.112180

Kazuhito Morioka , Toshiki Tazoe , J. Russell Huie , Kentaro Hayakawa , Rentaro Okazaki , Cristian F. Guandique , Carlos A. Almeida , Jenny Haefeli , Makoto Hamanoue , Takashi Endoh , Sakae Tanaka , Jacqueline C. Bresnahan , Michael S. Beattie , Toru Ogata , Adam R. Ferguson

{"title":"Disuse plasticity limits spinal cord injury recovery","authors":"Kazuhito Morioka , Toshiki Tazoe , J. Russell Huie , Kentaro Hayakawa , Rentaro Okazaki , Cristian F. Guandique , Carlos A. Almeida , Jenny Haefeli , Makoto Hamanoue , Takashi Endoh , Sakae Tanaka , Jacqueline C. Bresnahan , Michael S. Beattie , Toru Ogata , Adam R. Ferguson","doi":"10.1016/j.isci.2025.112180","DOIUrl":"10.1016/j.isci.2025.112180","url":null,"abstract":"<div><div>Use-dependent plasticity after spinal cord injury (SCI) enhances neuromotor function, however, the optimal timing to initiate rehabilitation remains controversial. To test impacts of early disuse, we established a rodent model of transient hindlimb suspension in acute phase SCI. Early disuse in the first 2-week after SCI undermined recovery on open-field locomotion, kinematics, and swim tests even after 6-week of normal gravity reloading. Early disuse produced chronic spinal circuit hyper-excitability in H-reflex and interlimb reflex tests. Quantitative synaptoneurosome analysis of lumboventral spinal cords revealed shifts in AMPA receptor (AMPAR) subunit GluA1 localization and serine 881 phosphorylation, reflecting enduring synaptic memories of early disuse stored in the spinal cord. Automated confocal analysis of motoneurons revealed persistent shifts toward GluA2-lacking, calcium-permeable AMPARs in disuse subjects. Unsupervised machine learning associated multidimensional synaptic changes with persistent recovery deficits in SCI. The results argue for early aggressive rehabilitation to prevent disuse plasticity that limits SCI recovery.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112180"},"PeriodicalIF":4.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of localized sustained-release drugs in periodontitis and comorbid diabetes: A systematic review and meta-analysis

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-07 DOI: 10.1016/j.isci.2025.112182

Jingru Zhuang (庄静茹) , Ying Ren (任颖) , Minmin Chen (陈敏敏) , Minghui Yue (岳明辉) , Changyong Yuan (袁长永) , Rongquan Duan (段荣泉)

{"title":"Efficacy of localized sustained-release drugs in periodontitis and comorbid diabetes: A systematic review and meta-analysis","authors":"Jingru Zhuang (庄静茹) , Ying Ren (任颖) , Minmin Chen (陈敏敏) , Minghui Yue (岳明辉) , Changyong Yuan (袁长永) , Rongquan Duan (段荣泉)","doi":"10.1016/j.isci.2025.112182","DOIUrl":"10.1016/j.isci.2025.112182","url":null,"abstract":"<div><div>Our meta-analysis aimed to evaluate the efficacy of localized sustained-release drugs in periodontitis and comorbid diabetes. PubMed, Cochrane Library, Embase, and Web of Science were comprehensively searched until 4 December 2024, and 10 randomized controlled trials (RCTs) were included. The results indicated that, compared to the control group, localized sustained-release drugs significantly reduced probing depth (PD) (SMD = −0.77, 95% confidence interval [CI] (−1.37, −0.16)) but did not reduce clinical attachment loss (CAL) (SMD = −0.18, 95% CI (−0.60, 0.23)), sites with glycated hemoglobin (HbA1c) (SMD = 0.03, 95% CI (−0.38, 0.43)), plaque index (SMD = −0.37, 95% CI (−0.80, 0.06)), sites with bleeding on probing (BOP) (SMD = −0.26, 95% CI (−0.68, 0.16)), and gingival index (SMD = 0.07, 95% CI (−0.30, 0.44)). Subgroup analysis by different drugs elicited that, compared to the control treatment, chlorhexidine was effective in reducing BOP% (SMD = −0.55, 95% CI (−0.90, −0.19)). Our meta-analysis finds that the efficacy of localized sustained-release drugs in periodontitis and comorbid diabetes is limited.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112182"},"PeriodicalIF":4.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DAP12-associated synthetic antigen receptors enable multi-targeting of T cells with independent chimeric receptors in a small genetic payload

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-07 DOI: 10.1016/j.isci.2025.112142

Allyson E. Moore , Hayley Nault , Derek Cummings , Bonnie Bojovic , Nick Serniuck , Christopher L. Baker , Craig Aarts , Chitra Venugopal , Sheila K. Singh , Joanne A. Hammill , Jonathan L. Bramson

{"title":"DAP12-associated synthetic antigen receptors enable multi-targeting of T cells with independent chimeric receptors in a small genetic payload","authors":"Allyson E. Moore , Hayley Nault , Derek Cummings , Bonnie Bojovic , Nick Serniuck , Christopher L. Baker , Craig Aarts , Chitra Venugopal , Sheila K. Singh , Joanne A. Hammill , Jonathan L. Bramson","doi":"10.1016/j.isci.2025.112142","DOIUrl":"10.1016/j.isci.2025.112142","url":null,"abstract":"<div><div>We describe a series of DAP12-associated receptors that can be used to achieve multi-targeting within a small genetic payload. Empirical evaluation of scaffold/binder combinations is required to define the optimal synthetic receptor configuration. When two DAP12-associated synthetic receptors were expressed in T cells from a single vector, the surface levels of individual receptors was reduced when compared to T cells engineered with vectors that express a single receptor. The reduction in receptor expression had a pronounced effect on early, but not late, signaling events and primarily affected cytokine production. The functional deficiency was overcome by increasing synthetic receptor levels demonstrating that there is no fundamental issue related to co-expression of multiple DAP12-associated synthetic receptors in a single T cell. Our data show that T cells can be engineered with multiple recombinant DAP12-based receptors to yield multi-target specific T cells, however, thoughtful design and optimization are necessary.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112142"},"PeriodicalIF":4.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polarization-insensitive narrowband reflective wavefront manipulation through all-dielectric anisotropic subwavelength structures

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-07 DOI: 10.1016/j.isci.2025.112147

Meiyan Pan , Yanxin Lu , Jintao Wang , Yihang Chen

{"title":"Polarization-insensitive narrowband reflective wavefront manipulation through all-dielectric anisotropic subwavelength structures","authors":"Meiyan Pan , Yanxin Lu , Jintao Wang , Yihang Chen","doi":"10.1016/j.isci.2025.112147","DOIUrl":"10.1016/j.isci.2025.112147","url":null,"abstract":"<div><div>Local metasurfaces provide high flexibility in shaping wavefronts but suffer from poor frequency selectivity. Here, we numerically present a reflective all-dielectric metasurface platform achieving simultaneous local phase control and spectral filtering: it reshapes the wavefront at a specific wavelength while spatially separating it from other wavelengths without requiring additional optics. The highly efficient phase control is inherently linked to the high polarization conversion ratio (PCR), ensured by a silicon nanoblock as a narrowband reflective half-wave plate through fundamental Mie resonances. The meta-atom exhibits near-unity peak reflection, a PCR reaching 0.97, and a quality factor around 20. By adjusting rotation angles, we disrupt the geometric phase conjugation, enabling polarization-insensitive applications, for example, a metalens with a high relative focusing efficiency (0.7) across the narrowband reflective spectrum. Our design exhibits high robustness against variations in the angle of incidence (up to 28<sup>°</sup>) and fabrication inaccuracies, allowing its implementation in various meta-applications.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112147"},"PeriodicalIF":4.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injured tubular epithelia-derived CCN1 promotes the mobilization of fibroblasts toward injury sites after kidney injury

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-07 DOI: 10.1016/j.isci.2025.112176

Tomohiro Nakata , Yuhei Kirita , Minato Umehara , Masashi Nakamura , Shinji Sawai , Atsushi Minamida , Hiroko Yamauchi-Sawada , Yasuto Sunahara , Yayoi Matoba , Natsuko Okuno-Ozeki , Itaru Nakamura , Kunihiro Nakai , Aya Yagi-Tomita , Noriyuki Yamashita , Keiichi Tamagaki , Benjamin D. Humphreys , Satoaki Matoba , Tetsuro Kusaba

{"title":"Injured tubular epithelia-derived CCN1 promotes the mobilization of fibroblasts toward injury sites after kidney injury","authors":"Tomohiro Nakata , Yuhei Kirita , Minato Umehara , Masashi Nakamura , Shinji Sawai , Atsushi Minamida , Hiroko Yamauchi-Sawada , Yasuto Sunahara , Yayoi Matoba , Natsuko Okuno-Ozeki , Itaru Nakamura , Kunihiro Nakai , Aya Yagi-Tomita , Noriyuki Yamashita , Keiichi Tamagaki , Benjamin D. Humphreys , Satoaki Matoba , Tetsuro Kusaba","doi":"10.1016/j.isci.2025.112176","DOIUrl":"10.1016/j.isci.2025.112176","url":null,"abstract":"<div><div>Humoral factors that prompt fibroblasts to migrate to an injury site at an appropriate time point are deemed indispensable for repair after kidney injury. We herein demonstrated the pivotal roles of injured tubule-derived cellular communication network factor 1 (CCN1) in the mobilization of fibroblasts to the injury site after kidney injury. Based on analyses of ligand-receptor interactions <em>in vitro</em> and tubular epithelial-specific transcriptomics <em>in vivo</em>, we identified the up-regulation of CCN1 during the early phases of kidney injury. CCN1 promotes fibroblast chemotaxis through focal adhesion kinase-extracellular signal-regulated kinase (ERK) signaling. <em>In</em> <em>vivo</em> analyses utilizing tubular-specific CCN1 knockout (KO) mice demonstrated the sparse accumulation of fibroblasts around injured sites after injury, resulting in ameliorated tissue fibrosis in CCN1-KO mice. These results reveal an epithelial-fibroblast CCN1 signaling axis that mobilizes fibroblasts to injured tubule early after acute injury but that promotes interstitial fibrosis at late time points.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112176"},"PeriodicalIF":4.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IR defect detection in metals

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-07 DOI: 10.1016/j.isci.2025.111984

Chunming Ai , Haichuan Lin , Pingping Sun , Xin Zhang

引用次数: 0

CCL7 promotes macrophage polarization and synovitis to exacerbate rheumatoid arthritis

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-03-07 DOI: 10.1016/j.isci.2025.112177

Jun Chen , Shuo Shi , Xiaojia Li , Feng Gao , Xu Zhu , Ru Feng , Ke Hu , Yicheng Li , Shuiyuan Chen , Rongkai Zhang , Xiaoshuai Wang , Changhai Ding , Gang Liu , Tianyu Chen , Wenquan Liang

{"title":"CCL7 promotes macrophage polarization and synovitis to exacerbate rheumatoid arthritis","authors":"Jun Chen , Shuo Shi , Xiaojia Li , Feng Gao , Xu Zhu , Ru Feng , Ke Hu , Yicheng Li , Shuiyuan Chen , Rongkai Zhang , Xiaoshuai Wang , Changhai Ding , Gang Liu , Tianyu Chen , Wenquan Liang","doi":"10.1016/j.isci.2025.112177","DOIUrl":"10.1016/j.isci.2025.112177","url":null,"abstract":"<div><div>Chemokine C-C motif ligand 7 (CCL7) is implicated in various immune and inflammatory processes; however, its role in rheumatoid arthritis (RA) remains unclear. In this study, we observed that CCL7 expression was upregulated in synovial M1-polarized macrophages and in the serum of RA mice and patients. CCL7 was found to promote macrophage polarization toward the M1 phenotype while inhibiting M2 differentiation <em>in vitro</em>. Furthermore, intra-articular injection of recombinant CCL7 protein in mice resulted in enhanced M1 polarization, increased inflammation, and fibrosis within synovial tissues, which exacerbated arthritis-associated pain. These effects were partially mitigated by treatment with a CCL7 neutralizing antibody. Mechanistically, we identified a CCL7 autocrine positive feedback loop that amplifies inflammation via the CCL7-CCR1-JAK2/STAT1 pathway. Collectively, our findings reveal a previously unrecognized CCL7-mediated autocrine inflammatory amplification loop that modulates macrophage polarization and exacerbates RA progression, positioning CCL7 as a potential therapeutic target for RA.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 4","pages":"Article 112177"},"PeriodicalIF":4.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0