iScience最新文献_第4页

Dynamic economic-entropy regulation of community-scale green hydrogen supply chains with carbon-microgrid coupling. 碳微网耦合下社区规模绿色氢供应链的动态经济熵调控

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115504

Guohui Lan, Yashu Chen, Chunzhong Li, Jianming Wang

引用次数: 0

Phylogenomic and evolutionary analysis of arrowhead (Sagittaria L.) chloroplast genomes. 箭头（Sagittaria L.）叶绿体基因组的系统基因组学和进化分析。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115487

Weiwei Zhao, Feng Gao, Yiling Wei, Youxuan Zhang, Niyan Xiang, Qiudi Zhang, Chenhui Zou, Yingming Duan, Yating Zhou, Tao Yuan, Feng Jiang

{"title":"Phylogenomic and evolutionary analysis of arrowhead (Sagittaria L.) chloroplast genomes.","authors":"Weiwei Zhao, Feng Gao, Yiling Wei, Youxuan Zhang, Niyan Xiang, Qiudi Zhang, Chenhui Zou, Yingming Duan, Yating Zhou, Tao Yuan, Feng Jiang","doi":"10.1016/j.isci.2026.115487","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115487","url":null,"abstract":"This study employed high-throughput sequencing to assemble and annotate the complete chloroplast genomes of three Sagittaria species. These genomes showed a conserved quadripartite structure, with length (∼178,339 bp) and GC content (36.8%-36.9%) exceeding those of other Alismataceae genera. Comparative analysis revealed structural conservation but species-specific variations at the JLB and JSA junctions. We identified numerous SNPs and SSRs across five species and found the rps16 gene and trnT-trnL spacer to be hypervariable, suggesting them as molecular markers. Codon usage bias indicated limited mutational pressure. Selective pressure analysis detected positive selection in clpP and ycf2. Phylogenetic analysis strongly supported Caldesia as sister to Sagittaria and placed S. platyphylla basally. Divergence time estimation suggested that Sagittaria and Caldesia split approximately 23.53 million years ago (Mya), while the internal diversification of Sagittaria occurred around 4.82 Mya. Our findings provide invaluable genomic resources and deep insights into the evolution, systematics, and potential genetic improvement of Sagittaria, which has significant ecological and industrial applications.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115487"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A distinct TCR⁺CD19⁺ population in the peritoneal cavity: B1a cells as precursors of extrathymic T cells. 腹膜腔中不同的TCR+CD19+群体：B1a细胞是胸腺外T细胞的前体。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115505

Su-Min Yee, Jeong-Eun Seon, Jae-Eun Seo, Hae-Jin Seo, Ja-Hun Seo, Seo-Hee Moon, Hyun-Su Koo, Ha-Rim Choi, Su-Man Kim, Hyung-Sik Kang

{"title":"A distinct TCR+CD19+ population in the peritoneal cavity: B1a cells as precursors of extrathymic T cells.","authors":"Su-Min Yee, Jeong-Eun Seon, Jae-Eun Seo, Hae-Jin Seo, Ja-Hun Seo, Seo-Hee Moon, Hyun-Su Koo, Ha-Rim Choi, Su-Man Kim, Hyung-Sik Kang","doi":"10.1016/j.isci.2026.115505","DOIUrl":"10.1016/j.isci.2026.115505","url":null,"abstract":"The precise ontogeny of extrathymic T cells remains elusive, and the potential contribution of self-renewing B1 cells to T cell generation has not been defined. Here, we identify a distinct population of TCR+CD19+ double-positive (DP) cells in the peritoneal cavity that serves as a developmental intermediate between B1a cells and extrathymic T cells. These DP cells originated from CD19+ B lineage cells and were highly enriched within the CD5+CD43+ B1a subset. Phenotypic and t-SNE analyses revealed ordered transitional states characterized by progressive acquisition of T lineage features. In vivo adoptive transfer and in vitro co-culture assays demonstrated that B1a cells generated functional extrathymic T cells, a process promoted by Notch signaling and IL-2 family cytokines. During this process, B1a-derived cells underwent transient Rag reactivation and TCR rearrangement and acquired canonical effector functions, including cytokine production and cytotoxicity. These findings uncover B1a lineage plasticity and define an unrecognized pathway of extrathymic T lymphopoiesis.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115505"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence-driven high-content imaging decodes for NLRP7-mutant recurrent hydatidiform moles. nlrp7突变体复发性葡萄胎的人工智能驱动高含量成像解码。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115479

Jiayang Wan, Tianliang Li, Limeng Cai, Yating Zhao, Jianhua Qian

{"title":"Artificial intelligence-driven high-content imaging decodes for NLRP7-mutant recurrent hydatidiform moles.","authors":"Jiayang Wan, Tianliang Li, Limeng Cai, Yating Zhao, Jianhua Qian","doi":"10.1016/j.isci.2026.115479","DOIUrl":"10.1016/j.isci.2026.115479","url":null,"abstract":"Recurrent hydatidiform moles (RHMs) are a gestational disorder primarily caused by maternal-effect loss-of-function mutations in NLRP7. This study established an in vitro model of NLRP7 mutation using patient-derived induced pluripotent stem cells (iPSCs) and integrated high-content imaging (HCI) with artificial intelligence (AI) to dissect mutation-induced multi-organellar dysfunction. Multiparametric HCI enabled synchronous capture of cellular and subcellular phenotypes, including mitochondrial function and lysosomal distribution. We developed a bio-inspired AI framework (BioVision-Segmentation) that combines a hybrid Transformer-Voronoi architecture for segmentation with mutual information analysis to quantify organelle interactions. Our findings reveal that NLRP7 mutations disrupt the lysosome-mitochondria crosstalk hub, leading to energy metabolism dysregulation, reactive oxygen species (ROS) accumulation, and organelle spatial distribution defects. Furthermore, transcriptome sequencing corroborated these findings. This study elucidates the organellar pathogenesis of RHMs and provides a technological platform for exploring therapeutic targets, facilitating a shift toward early embryo protection strategies.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115479"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STARR-CRAAVT: A platform to identify cell type-specific regulatory elements for next-generation gene therapy. STARR-CRAAVT：用于下一代基因治疗的细胞类型特异性调控元件鉴定平台。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-25 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115469

Robert Becker, Priyanka Choudhury, Martin Oti, Youssef Fouani, Benjamin Strobel, Stephanie Ketterer, Simon Rumpel, John Park, Sebastian Kreuz, Udo Maier, Stefan Michelfelder, Christian Schön

{"title":"STARR-CRAAVT: A platform to identify cell type-specific regulatory elements for next-generation gene therapy.","authors":"Robert Becker, Priyanka Choudhury, Martin Oti, Youssef Fouani, Benjamin Strobel, Stephanie Ketterer, Simon Rumpel, John Park, Sebastian Kreuz, Udo Maier, Stefan Michelfelder, Christian Schön","doi":"10.1016/j.isci.2026.115469","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115469","url":null,"abstract":"Designing enhancer-promoter combinations that enable potent and precise transgene expression is a promising strategy for advancing gene therapy mediated by adeno-associated virus (AAV). We introduce STARR-CRAAVT (self-transcribing active regulatory region sequencing of captured libraries for rAAV gene therapy), a STARR-Seq-based platform using in silico tailored sequence libraries to screen for enhancers in AAVs and assess relevant applications and critical screening parameters in an in vitro proof-of-concept study. Candidate libraries were generated by integrating epigenetic data, followed by the capture of candidate-containing genomic fragments and packaging into AAVs. Using STARR-CRAAVT, we identified enhancers driving cell type-specific expression and showed that the promoter type is a key determinant for the ability of candidates to act as enhancers. Notably, we also found that switching the candidate position in the AAV genome can significantly influence enhancer activity. Our study yields insights into enhancer function and describes a blueprint for the implementation of STARR-CRAAVT to identify cell type-specific enhancers for AAV-based gene therapy.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115469"},"PeriodicalIF":4.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Socioeconomic status influenced dispersal in early adulthood in Finland from 1760 to 1969. 从1760年到1969年，社会经济地位影响了芬兰成年早期的分散。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-25 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115467

Jenni J Kauppi, Alyona Artamonova, Milla Salonen, Mirkka Lahdenperä, Virpi Lummaa

{"title":"Socioeconomic status influenced dispersal in early adulthood in Finland from 1760 to 1969.","authors":"Jenni J Kauppi, Alyona Artamonova, Milla Salonen, Mirkka Lahdenperä, Virpi Lummaa","doi":"10.1016/j.isci.2026.115467","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115467","url":null,"abstract":"Dispersal away from the place of birth shapes an individual's life course and has effects on the demography of populations. Parental socioeconomic status (SES) might shape dispersal decisions of young individuals by providing resources that enable dispersal or philopatry. High familial wealth can allow young adults to remain in their birth place or, in contrast, provide necessary resources to disperse. Using a large demographic dataset from Finland (1760-1969), we examined how parental SES influenced both the probability and distance of dispersal among young men and women over time. Individuals from high-SES families were more likely to remain in their birth parishes than those from low-SES background. Across the study period, both the likelihood and distance of dispersal increased, reflecting the broader societal transitions. Our findings highlight how socioeconomic resources and historical changes impact dispersal behavior, revealing disparities in how such changes affect young adults with differential access to parental resources.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115467"},"PeriodicalIF":4.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of clearing methods for 3D imaging of the vasculature in mineralized mouse tissues. 矿化小鼠组织血管三维成像清除方法的比较分析。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-25 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115464

Azeez O Ishola, Athira Pillai, Taeyong Ahn, Chih-Wei Hsu, Brendan Lee, Nele Haelterman, Joshua D Wythe

{"title":"Comparative analysis of clearing methods for 3D imaging of the vasculature in mineralized mouse tissues.","authors":"Azeez O Ishola, Athira Pillai, Taeyong Ahn, Chih-Wei Hsu, Brendan Lee, Nele Haelterman, Joshua D Wythe","doi":"10.1016/j.isci.2026.115464","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115464","url":null,"abstract":"Historically, visualization of vascular networks within the musculoskeletal system, particularly in mineralized tissues such as bones and joints, has been limited to conventional 2D histological approaches. Recent advances in optical tissue clearing technologies and fluorescence imaging approaches in whole, intact tissues offer an entrée to interrogate the vasculature at unprecedented resolution during both musculoskeletal development and in pathologic contexts in three dimensions. However, these clearing techniques were originally not developed for imaging hard mineralized tissues, such as the femur and tibia. Herein, we have optimized tissue decalcification conditions and compared aqueous- and solvent-based tissue clearing approaches to establish an optimal pipeline for clearing and imaging the vasculature in mineralized tissues of the adult mouse. Collectively, this work shows that optical clearing combined with light-sheet microscopy represents a powerful method for generating high-resolution images of the murine hindlimb vasculature, with potential applications in aging and disease modeling.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115464"},"PeriodicalIF":4.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the chemical defenses of an invasive toad indicate drivers and limitations of adaptation. 入侵蟾蜍化学防御的变化表明了适应的驱动因素和限制。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-25 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115401

Max Mühlenhaupt, James Baxter-Gilbert, Julia L Riley, Buyisile G Makhubo, Nhlanhla S Dludla, Cláudia Baider, F B Vincent Florens, Xavier Porcel, André de Villiers, Willem A L van Otterlo, John Measey

{"title":"Changes in the chemical defenses of an invasive toad indicate drivers and limitations of adaptation.","authors":"Max Mühlenhaupt, James Baxter-Gilbert, Julia L Riley, Buyisile G Makhubo, Nhlanhla S Dludla, Cláudia Baider, F B Vincent Florens, Xavier Porcel, André de Villiers, Willem A L van Otterlo, John Measey","doi":"10.1016/j.isci.2026.115401","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115401","url":null,"abstract":"Chemical defenses of invasive species provide protection against predators, competitors, and parasites enabling successful colonization of novel environments and can be highly detrimental to native biota. Here, we study how the chemical defenses of a successful invasive toad have changed by comparing the native source population with the introduced populations on Mauritius, Réunion, and in Cape Town. In line with the enemy release hypothesis, on Mauritius and Réunion where the community of predator species is less diverse, the toads show marked reductions in toxin gland size as well as changes in the composition of the toxins. However, in Cape Town, where the introduction happened more recently and the predator community is more diverse, only the composition of the toad toxins changed. Our results indicate drivers and constraints of adaptations of chemical defenses and provide evidence for the role of enemy release in the success of an invasive toad.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115401"},"PeriodicalIF":4.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-25 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115474

Paul Jonas Jost, Daniel Weindl, Klaus Wunderling, Christoph Thiele, Jan Hasenauer

{"title":"Suggested experimental design and computational modeling to infer single-cell lipid dynamics from a single destructive measurement.","authors":"Paul Jonas Jost, Daniel Weindl, Klaus Wunderling, Christoph Thiele, Jan Hasenauer","doi":"10.1016/j.isci.2026.115474","DOIUrl":"10.1016/j.isci.2026.115474","url":null,"abstract":"Cellular heterogeneity is a fundamental facet of cell biology, influencing cellular signaling, metabolism, and gene regulation. Its accurate quantification requires measurements at the single-cell level. Most high-throughput single-cell technologies provide only a snapshot of cellular heterogeneity at a specific time point because the measurement is destructive. This limits our current ability to understand the dynamics of cellular behavior and quantify cell-specific parameters. We propose a potential experimental setup combined with a model-based analysis framework, enabling the extraction of longitudinal data from a single destructive measurement. Although broadly applicable, we focus on lipid metabolism, a domain where obtaining longitudinal single-cell data have remained elusive due to technical constraints. Our method leverages multiple labels whose measurements are linked to a shared dynamic. This allows the estimation of cell-specific parameters and the quantification of heterogeneity. This framework establishes a foundation for future investigations, providing a roadmap toward a deeper understanding of dynamic cellular processes.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115474"},"PeriodicalIF":4.1,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OSBPL2 deficiency alleviates diet-induced MASLD by reducing ACSL4-mediated ferroptosis. OSBPL2缺乏通过减少acsl4介导的铁下垂来减轻饮食诱导的MASLD。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-25 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115478

Tianming Wang, Fanghong Sheng, Chao Lian, Zhengyuan Lv, Jun Yao, Xin Cao, Xuemei Chen, Jianquan Chen

引用次数: 0