iScience最新文献_第3页

Chromosome-level genome of Madhuca hainanensis reveals genomic evolution and floral divergence in Madhuca. 海南麻的染色体水平基因组揭示了麻的基因组进化和花的分化。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-27 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115480

Yifei Ma, Ziyao Wang, Jingzhe Qiu, Shuiyun Ye, Guangzhou Chen, Jiayu Qin, Lu Zhang

引用次数: 0

Alterations in brain spatiotemporal autocorrelation and functional connectivity in lifelong premature ejaculation: A resting-state fMRI study. 终身早泄的大脑时空自相关和功能连通性的改变：静息状态fMRI研究。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-27 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115509

Qiming Deng, Xin Zhang, Qingqiang Gao, Cong Wang, Xiaoyan Wu, Jiaming Lu, Bing Zhang

{"title":"Alterations in brain spatiotemporal autocorrelation and functional connectivity in lifelong premature ejaculation: A resting-state fMRI study.","authors":"Qiming Deng, Xin Zhang, Qingqiang Gao, Cong Wang, Xiaoyan Wu, Jiaming Lu, Bing Zhang","doi":"10.1016/j.isci.2026.115509","DOIUrl":"10.1016/j.isci.2026.115509","url":null,"abstract":"Spatial and temporal autocorrelation, as low-dimensional statistical properties, account for a substantial portion of variance in complex functional brain network topology, thereby providing a method for understanding the architecture of brain functional organization in specific populations. In this study, we investigated lifelong premature ejaculation (LPE) by analyzing spatiotemporal autocorrelation and observed increased temporal autocorrelation in the right thalamus, left prefrontal cortex, and left somatomotor cortex, which may reflect abnormal neural signal persistence. Seed-based functional connectivity (FC) analysis further indicated increased FC in the right inferior parietal lobule, left anterior cingulate cortex, and right precuneus, alongside decreased FC in the right superior occipital gyrus. These FC alterations showed significant correlations with spatial autocorrelation metrics. FC in the right precuneus was positively associated with ejaculatory latency and patient-reported control, and negatively correlated with clinical scores. These disrupted spatiotemporal dynamics within prefrontal-somatosensory and occipital-limbic networks suggest that autocorrelation patterns may represent a promising neural correlate of LPE, offering new insights into its underlying neural mechanisms.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115509"},"PeriodicalIF":4.1,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mathematical modeling of ribosome competition informs testable treatment strategies for drug-tolerant cancer persister cells. 核糖体竞争的数学模型为耐药癌症持久细胞的可测试治疗策略提供了依据。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-27 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115493

Xinpu Tang, Yuqing Wang, Yi Pu, Kaixiu Li, Zheyu Ding, Mengyao Wang, Luis Almeida, Michael Cerezo, Yarong Cao, Caroline Robert, Diane Peurichard, Shensi Shen

{"title":"Mathematical modeling of ribosome competition informs testable treatment strategies for drug-tolerant cancer persister cells.","authors":"Xinpu Tang, Yuqing Wang, Yi Pu, Kaixiu Li, Zheyu Ding, Mengyao Wang, Luis Almeida, Michael Cerezo, Yarong Cao, Caroline Robert, Diane Peurichard, Shensi Shen","doi":"10.1016/j.isci.2026.115493","DOIUrl":"10.1016/j.isci.2026.115493","url":null,"abstract":"Systemic therapies for advanced cancers often induce initial responses but rarely achieve durable cures due to acquired resistance. Drug-tolerant persister (DTP) cells survive treatment without additional genetic mutations. We previously showed that melanoma DTP cells globally suppress mRNA translation while selectively maintaining translation of specific mRNAs, but the basis of this selectivity remained unclear. Here, we integrate stochastic modeling with experimental analyses to define the principles governing selective translation in DTP cells. We identify translational reprogramming as a conserved feature of DTP cells across cancer types and treatments. Reduced MYC-dependent ribosome biogenesis limits ribosome availability, creating a translational bottleneck. Modeling reveals that ribosome scarcity drives competition among mRNAs, thereby shaping selective translation. This framework uncovers a ribosome-dependent survival checkpoint in DTP cells and highlights ribosome thresholds as a potential vulnerability for overcoming therapy resistance.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115493"},"PeriodicalIF":4.1,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust single-molecule tracking of RNA abundance and dynamics via TSSOT. 强大的单分子跟踪RNA丰度和动态通过TSSOT。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-27 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115503

Yulin Luo, Yujuan Fu, Jianghu Wang, Sihui Li, Tianyi Cheng, Chengying Pi, Chuhan Qiao, Wei Zou, Nan Liu, Xiaoyue Wang, Baohui Chen

{"title":"Robust single-molecule tracking of RNA abundance and dynamics via TSSOT.","authors":"Yulin Luo, Yujuan Fu, Jianghu Wang, Sihui Li, Tianyi Cheng, Chengying Pi, Chuhan Qiao, Wei Zou, Nan Liu, Xiaoyue Wang, Baohui Chen","doi":"10.1016/j.isci.2026.115503","DOIUrl":"10.1016/j.isci.2026.115503","url":null,"abstract":"Single-RNA imaging in living cells offers precise insights into the spatiotemporal regulation of gene expression. However, achieving stable, efficient, and reproducible RNA labeling remains challenging. To address this, we developed TSSOT (tandem split-mNeonGreen-based signal-optimized mRNA tracking), a robust live-cell imaging system that ensures consistent RNA labeling across multiple cell passages and provides highly reproducible, quantitative measurements at single-molecule resolution within individual cells. Leveraging TSSOT, we assessed the efficacy of various gene manipulation techniques and identified critical factors influencing Cas13d-mediated RNA cleavage. TSSOT further demonstrated that downregulation of mRNA export factors NXF1 and ALYREF leads to profound nuclear mRNA export defects. Furthermore, we employed TSSOT to spatiotemporally resolve the dynamic redistribution of mRNAs during stress granule formation. By providing precise quantification of RNA abundance, spatial organization, and temporal behavior, TSSOT is a valuable tool for live-cell RNA analysis, contributing to a better understanding of the dynamic RNA landscape.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115503"},"PeriodicalIF":4.1,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photocatalytic performance and mechanism of oxygen-doped g-C₃N₄ and photoactivated peroxymonosulfate on rhodamine B. 氧掺杂g-C3N4和光活化过氧单硫酸盐对罗丹明B的光催化性能及机理

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-27 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115405

Xiaocan Liu, Weiye Yang, Hongyan Peng, Lijian Meng, Shihua Zhao

{"title":"Photocatalytic performance and mechanism of oxygen-doped g-C3N4 and photoactivated peroxymonosulfate on rhodamine B.","authors":"Xiaocan Liu, Weiye Yang, Hongyan Peng, Lijian Meng, Shihua Zhao","doi":"10.1016/j.isci.2026.115405","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115405","url":null,"abstract":"Organic pollutants such as dyes and pharmaceuticals pose serious threats to aquatic ecosystems and human health, highlighting the need for efficient advanced oxidation processes. Here, oxygen-doped graphitic carbon nitride was synthesized by co-calcination of melamine and cyanuric acid and applied to a photoactivated peroxymonosulfate system. Comprehensive characterizations combined with density functional theory calculations revealed that oxygen incorporation induces charge redistribution and improves the surface structure, leading to enhanced visible light absorption and charge separation. Under simulated sunlight, the catalyst achieved 99% degradation of rhodamine B (30 mg/L) within 6 min, with a reaction rate 14 times higher than that with pristine carbon nitride. Reactive species trapping and electron paramagnetic resonance analyses confirmed that singlet oxygen and superoxide radicals dominate the degradation process. The catalyst also exhibited good stability over five cycles. This work provides an effective strategy for designing metal-free photocatalysts for peroxymonosulfate activation and wastewater treatment.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115405"},"PeriodicalIF":4.1,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organization of the ctenophore tentacular apparatus: Adult Mnemiopsis leidyi lacks a canonical principal tentacle. 栉水母触须器官的组织：成年雷氏记忆藓缺少一个规范的主触须。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115508

Gen Dong, Dorothy Mitchell, Anthony Moss

{"title":"Organization of the ctenophore tentacular apparatus: Adult Mnemiopsis leidyi lacks a canonical principal tentacle.","authors":"Gen Dong, Dorothy Mitchell, Anthony Moss","doi":"10.1016/j.isci.2026.115508","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115508","url":null,"abstract":"Ctenophores represent one of the earliest-diverging animal lineages and are distinguished by their exceptional feeding efficiency. The tentacular apparatus of Mnemiopsis leidyi mediates feeding and likely serves diverse sensory roles, yet its structure remains poorly resolved, with conflicting accounts in the literature. Here, we clarify its organization through anatomical and experimental analyses. Light microscopy shows that tentilla bundles arise from a fan-shaped structure previously misidentified as a vestigial principal tentacle. Each tentillum connects directly to the aboral end of the tentacular bulb, contradicting earlier embedding models. Cryosectioning and lesion experiments define the origin and migration of tentilla, while videography reveals fan swaying that bilaterally distributes them. We further show that tentilla can be recycled via entrapment in the transport groove and redirected to the mouth. These findings provide an updated model for understanding tentillar growth, distribution, transport, and recycling within the feeding apparatus.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115508"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural evidence that RNA contributes to polymorphism of tau amyloid fibrils. 结构证据表明RNA有助于tau淀粉样蛋白原纤维的多态性。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115501

Romany Abskharon, Yi Xiao Jiang, Michael R Sawaya, Peng Ge, Jeffrey Zhang, David R Boyer, Joshua L Dolinsky, Justin Pi, Duilio Cascio, Feng Guo, David S Eisenberg

{"title":"Structural evidence that RNA contributes to polymorphism of tau amyloid fibrils.","authors":"Romany Abskharon, Yi Xiao Jiang, Michael R Sawaya, Peng Ge, Jeffrey Zhang, David R Boyer, Joshua L Dolinsky, Justin Pi, Duilio Cascio, Feng Guo, David S Eisenberg","doi":"10.1016/j.isci.2026.115501","DOIUrl":"10.1016/j.isci.2026.115501","url":null,"abstract":"RNA colocalizes with tau deposits in Alzheimer's disease (AD) and drives tau aggregation in vitro. Previously, we determined a cryogenic-electron microscopy (cryo-EM) structure of fibrils of full-length tau bound to unfractionated mammalian RNA, revealing a small tau C-terminal core. Here, we present the cryo-EM structure of fibrils of full-length recombinant tau bound to unfractionated mammalian RNA seeded by AD-extracted tau fibrils. This structure reveals an expanded tau C-terminal core resembling AD tau fibrils. RNA sequencing identified 18S ribosomal RNA as the primary fibril-bound species. Next, we determined the cryo-EM structure of fibrils of full-length recombinant tau bound to mammalian 18S ribosomal RNA, revealing a core that consists of the R2 to R4 repeat domains previously seen in pathological tau fibrils. All our recombinant RNA-tau fibrils dissolve upon RNase treatment. Tau fibrils adopt distinct folds in the presence of different RNAs, suggesting RNA is a cofactor capable of shaping tau fibril polymorphism.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115501"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical innovations in the assembly of the modern flight apparatus in Early Cretaceous birds. 早白垩纪鸟类现代飞行装置组装的关键创新。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115506

Qian Wu, Thomas A Stidham, Jingmai K O'Connor, Alida M Bailleul, Zhonghe Zhou, Zhiheng Li

{"title":"Critical innovations in the assembly of the modern flight apparatus in Early Cretaceous birds.","authors":"Qian Wu, Thomas A Stidham, Jingmai K O'Connor, Alida M Bailleul, Zhonghe Zhou, Zhiheng Li","doi":"10.1016/j.isci.2026.115506","DOIUrl":"10.1016/j.isci.2026.115506","url":null,"abstract":"A pivotal innovation in the evolution of powered flight in dinosaurs was the triosseal canal-a specialized passage formed by the scapula, coracoid, and furcula that guides the wing-elevation tendon. However, the origins of this structure remained obscure. Here, we applied integrated histological analysis and micro-computed tomography (CT) scanning of a new enantiornithine specimen and the basal ornithuromorph Archaeorhynchus. Our results indicate that the triosseal canal evolved first through paedomorphosis of the coracoscapular joint into a synchondrosis in ornithothoracines and subsequent acquisition of the acrocoracoclavicular joint in ornithuromorphs. The complete lack of a connection between the furcula and coracoid represents one of the crucial skeletal disparities between enantiornithines and ornithuromorphs. We propose that the closure of the triosseal canal in ornithuromorphs markedly improved tendon stability, facilitating a greater range of wing motion and more efficient flight compared to enantiornithines, serving as one of the critical functional triggers responsible for their ecological diversification.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115506"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13091450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding unchanged transcriptome of Alzheimer's disease reveals an NCAM1 mRNA switch as a potential biomarker. 解码阿尔茨海默病未改变的转录组揭示了NCAM1 mRNA开关作为潜在的生物标志物。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115456

Haotian Li, Sai Liu, Daoxin Miao, Long Chen, Yuan Sun, Guangji Wang, Zheying Zhu, Xinuo Li, Qiulun Lu

{"title":"Decoding unchanged transcriptome of Alzheimer's disease reveals an NCAM1 mRNA switch as a potential biomarker.","authors":"Haotian Li, Sai Liu, Daoxin Miao, Long Chen, Yuan Sun, Guangji Wang, Zheying Zhu, Xinuo Li, Qiulun Lu","doi":"10.1016/j.isci.2026.115456","DOIUrl":"https://doi.org/10.1016/j.isci.2026.115456","url":null,"abstract":"Alzheimer's disease (AD), a neurodegenerative disease primarily affecting older adults, is characterized by changes in memory, behavior, and language. Although gene expression varies during AD progression, the molecular mechanisms underlying this variation remain unclear. RNA sequencing indicates that most genes exhibit minimal gene-level differential expression in AD but may relate to neuronal function. Our comprehensive analysis revealed that neural cell adhesion molecule 1 (NCAM1) underwent alternative splicing (AS) in AD. Notably, an isoform switch occurred from the long isoform (L-NCAM1), typical under normal conditions, to the short isoform (S-NCAM1) in AD. S-NCAM1 lacked the intracellular domain in L-NCAM1. Additionally, the S-NCAM1-to-L-NCAM1 ratio increased in the hippocampus of amyloid precursor protein (APP)/PS1 mice compared to wild-type mice. Single-nucleus sequencing determined that this change in NCAM1 isoforms occurred predominantly within reactive astrocytes. Hence, AS may play a key role in AD development, while the L-NCAM1-to-S-NCAM1 ratio could serve as a biomarker.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 4","pages":"115456"},"PeriodicalIF":4.1,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13090632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147721286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating age-related stereo decline with perceptual training. 通过知觉训练减轻与年龄相关的立体感下降。

IF 4.1 2区综合性期刊

iScience Pub Date : 2026-03-26 eCollection Date: 2026-04-17 DOI: 10.1016/j.isci.2026.115485

Ka Yee Kam, Leo C H Ng, Dorita H F Chang

引用次数: 0