Spatiotemporal dynamics of NF-κB/Dorsal inhibitor IκBα/Cactus in Drosophila blastoderm embryos

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-06-09 DOI:10.1016/j.isci.2025.112854

Allison E. Schloop , Sharva Vijayakumar Hiremath , Razeen Shaikh , Sadia Siddika Dima , Leslie Lizardo , Amrit Bhakta , Cranos M. Williams , Gregory T. Reeves

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Abstract

The NF-κB/IκBα pathway regulates key cellular processes, including development, immunity, and inflammation. In Drosophila, the homologous Dorsal/Cactus system patterns the dorsoventral axis of the blastoderm embryo. While the inhibitor Cactus has been suggested to play multiple roles in regulating Dorsal, direct visualization of Cactus is challenging due to its rapid turnover. To overcome this challenge, we endogenously tagged Cactus with a nanobody against GFP and quantitatively imaged Cactus distribution in live embryos. We found that, like Dorsal, Cactus undergoes nuclear-cycle driven dynamics and nucleocytoplasmic shuttling. Using a mathematical model constrained by our data, we estimated Cactus nuclear and cytoplasmic concentration. Our results imply that the Dorsal gradient has increased spatial range, robustness, and precision due to the presence of Cactus in the nucleus uniformly throughout the embryo. This study provides insights into NF-κB regulation in Drosophila embryos and highlights the need for similar quantitative studies in other biological systems.

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NF-κB/背侧抑制剂i -κB α/仙人掌在果蝇胚皮胚中的时空动态

NF-κB/ i -κB α通路调节关键的细胞过程，包括发育、免疫和炎症。在果蝇中，同源的背/仙人掌系统形成胚皮胚胎的背-腹轴。虽然抑制剂Cactus已被认为在调节Dorsal中发挥多种作用，但由于其快速的转换，直接可视化Cactus具有挑战性。为了克服这一挑战，我们用抗GFP的纳米体内源性标记仙人掌，并定量成像仙人掌在活胚胎中的分布。我们发现，仙人掌与背侧植物一样，也经历了核周期驱动动力学和核胞质穿梭。使用数学模型，我们的数据约束，我们估计仙人掌的核和细胞质浓度。我们的研究结果表明，由于仙人掌在整个胚胎中均匀地存在于细胞核中，背侧梯度增加了空间范围、鲁棒性和精度。该研究为果蝇胚胎中NF-κB的调控提供了见解，并强调了在其他生物系统中进行类似定量研究的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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