雄性特异性gaba能神经元中sifamide依赖的突触可塑性是经验调节交配行为的基础

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-09-05 DOI:10.1016/j.isci.2025.113516

Tianmu Zhang (张天目) , Hongyu Miao (苗鸿钰) , Yutong Song (宋雨桐) , Zekun Wu (吴泽坤) , Woo Jae Kim (金佑宰김우재)

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引用次数: 0

摘要

黑腹果蝇的性行为依赖于复杂的脑回路和神经调节，但神经肽信号在行为改进中的作用尚不清楚。我们研究了SIFamide （SIFa）及其受体（SIFaR）在调节雄性果蝇性行为中的作用。SIFaR对于这些行为至关重要，尤其是间隔计时。fru阳性神经元敲低会影响求偶指数（CI）和交配持续时间（MD），但不会影响交配潜伏期（CL）。关键是，gaba能mAL神经元（fru+, dsx−）中的SIFaR特异性控制MD和CI，而不是CL。此外，经验丰富的雄性mAL神经元中的SIFa信号增加了突触前终末，表明上下文依赖的突触可塑性。这些结果揭示了SIFa/SIFaR与特定神经元在调节男性行为中的复杂相互作用。SIFaR在frui阳性和其他神经元中的独特作用及其可塑性功能，为研究情境依赖行为适应的神经机制提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

SIFamide-dependent synaptic plasticity in male-specific GABAergic neurons underlies experience-modulated mating behaviors

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SIFamide-dependent synaptic plasticity in male-specific GABAergic neurons underlies experience-modulated mating behaviors

Drosophila melanogaster relies on complex brain circuits and neuromodulation for experience-dependent sexual behavior, yet the role of neuropeptide signaling in behavioral refinement remains unclear. We investigate SIFamide (SIFa) and its receptor (SIFaR) in regulating male Drosophila sexual behavior. SIFaR is essential for these behaviors, particularly interval timing. Knockdown in fru-positive neurons impairs courtship index (CI) and mating duration (MD) but not copulation latency (CL). Critically, SIFaR in GABAergic mAL neurons (fru⁺, dsx⁻) specifically controls MD and CI, not CL. Additionally, SIFa signaling in mAL neurons of experienced males increases presynaptic terminals, indicating context-dependent synaptic plasticity. These results reveal a complex SIFa/SIFaR interaction with specific neurons in modulating male behavior. The distinct roles of SIFaR in fru-positive versus other neurons, alongside its plasticity function, provide new insights into neural mechanisms of context-dependent behavioral adaptation.

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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