Insect Biochemistry and Molecular Biology最新文献

{"title":"The parasitoid Exorista sorbillans exploits host silkworm encapsulation to build respiratory funnel for survival","authors":"Qian Xu ,&nbsp;Jialei Lu ,&nbsp;Xinran Gu ,&nbsp;Fupeng Chi ,&nbsp;Yue Zhao ,&nbsp;Fanchi Li ,&nbsp;Xuejian Jiang ,&nbsp;Bing Li ,&nbsp;Jing Wei","doi":"10.1016/j.ibmb.2024.104255","DOIUrl":"10.1016/j.ibmb.2024.104255","url":null,"abstract":"<div><div>Insect parasitoids have evolved sophisticated strategies to evade or modulate host immunity for parasitic infections. The precise mechanisms by which parasitoids counteract host anti-parasitic responses are poorly defined. Here we report a novel immune evasion strategy employed by the parasitoid <em>Exorista sorbillans</em> (Diptera: Tachinidae) to establish infection. We find that <em>E. sorbillans</em> larva construct a respiratory funnel that gradually increases in size as development progresses. This respiratory funnel, which connect to the parasitoid invasion aperture on the host silkworm epidermis, proves essential for <em>E. sorbillans</em> development, as sealing the invasion aperture results in complete mortality of larval parasitoids. Our investigation reveals that <em>E. sorbillans</em> infection reduces both host silkworms' hemocyte counts and the expression of hemocyte-specific genes, while simultaneously inducing varying degrees of host silkworm encapsulation at different parasitic stages. Nevertheless, more complete inhibition of host silkworm encapsulation through RNAi leads to parasitoid's defective respiratory funnel formation and increased mortality rates of the parasitoid. Further observations demonstrate that this suppressed encapsulation response triggers an enhanced activation of Toll/IMD pathways in the host silkworm. Take together, we show that <em>E. sorbillans</em> may utilize host silkworm encapsulation to construct a respiratory funnel for both respiration and immune evasion. Our findings provide new insights into the evasion tactics employed by parasitoids win out in the ongoing parasite-host evolutionary arms race.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104255"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cis-regulation of the CYP6CS1 gene and its role in mediating cross-resistance in a pymetrozine-resistant strain of Nilaparvata lugens","authors":"Pin-Xuan Lin ,&nbsp;Yu-Xuan Peng ,&nbsp;Ji-Yang Xing ,&nbsp;Zhao-Yu Liu ,&nbsp;Fang-Rui Guo ,&nbsp;Joshua A. Thia ,&nbsp;Cong-Fen Gao ,&nbsp;Shun-Fan Wu","doi":"10.1016/j.ibmb.2025.104261","DOIUrl":"10.1016/j.ibmb.2025.104261","url":null,"abstract":"<div><div>Pymetrozine is currently one of the primary insecticides used to control the brown planthopper, <em>Nilaparvata lugens</em> Stål (Hemiptera: Delphacidae), but the long-term effectiveness of this chemical is threatened by growing issues of resistance. Previous studies in a laboratory selected strain of <em>N. lugens</em>, Pym-R, have shown that resistance to pymetrozine can evolve without target-site mutations. A key candidate gene identified is the cytochrome P450 gene <em>CYP6CS1</em>, which is overexpressed in the resistant Pym-R strain compared to the laboratory susceptible strain, Pym-S. In this study, we provide a deeper characterization of the regulatory mechanism and phenotypic effects of <em>CYP6CS1</em> by comparing the resistant and susceptible variants of this gene. Using artificial constructs in Luciferase activity assays, we elucidate the role of indels in the overexpression of <em>CYP6CS1</em> in the resistant strain. Additionally, transgenic <em>Drosophila</em> experiments also revealed that the <em>CYP6CS1</em> gene not only contributes to resistance against pymetrozine, but is able to confer moderate to low cross-resistance to several other pesticides. This research provides vital insights into the possible genetic mechanisms that may contribute to pymetrozine resistance in field populations. Future work will aim to examine the relevance of <em>CYP6CS1</em> variation in the field with the aim of developing diagnostic markers of resistance.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104261"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of tick attachment and rapid engorgement via dopamine receptors in the Asian longhorned tick Haemaphysalis longicornis","authors":"Seoyul Hwang ,&nbsp;Donghun Kim","doi":"10.1016/j.ibmb.2025.104262","DOIUrl":"10.1016/j.ibmb.2025.104262","url":null,"abstract":"<div><div>Dopamine plays multifaceted roles in the physiology of insects and ticks, acting as a key neurotransmitter and modulator of various biological processes. In ticks, it plays a particularly important role in regulating salivary gland function, which is essential for successful tick feeding on hosts. Salivary secretion in ticks is orchestrated by the collection of saliva in the acinar lumen mediated by the dopamine receptor (D1) and the expulsion of collected saliva into the salivary duct mediated by the invertebrate specific D1-like dopamine receptor (InvD1L). However, the function of dopamine receptors in different feeding stages and other tissues remains unclear. In this study, D1 and InvD1L of <em>Haemaphysalis longicornis</em> (Haelo-D1 and Haelo-InvD1L, respectively) were found to be involved in tick attachment and the rapid phase of blood feeding. Both receptors were identified and profiled in the synganglion, salivary glands, and midgut of <em>H. longicornis</em> females across different feeding stages. Functional analyses revealed that both receptors were activated by dopamine in a concentration-dependent manner with distinct sensitivities. RNA interference (RNAi) targeting these receptors significantly reduced dopamine-mediated salivation and delayed tick attachment and blood feeding. Furthermore, RNAi prolonged rapid engorgement phases and reduced the final body weight of replete ticks. These results highlight the crucial roles of D1 and InvD1L in regulating salivary secretion in ixodid ticks and facilitating their attachment and rapid engorgement, thereby offering potential targets for novel tick control strategies aimed at disrupting feeding and reducing pathogen transmission.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104262"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest","authors":"Xuanhao Chen ,&nbsp;Shuyuan Yao ,&nbsp;Liangqian Xie ,&nbsp;Jinyang Li ,&nbsp;Lei Xiong ,&nbsp;Xiaozhen Yang ,&nbsp;Yi Chen ,&nbsp;Fang Cao ,&nbsp;Qing Hou ,&nbsp;Minsheng You ,&nbsp;Yuanyuan Liu ,&nbsp;Geoff M. Gurr ,&nbsp;Shijun You","doi":"10.1016/j.ibmb.2024.104248","DOIUrl":"10.1016/j.ibmb.2024.104248","url":null,"abstract":"<div><div>The olfactory system of insects plays a pivotal role in multiple, essential activities including feeding, mating, egg laying, and host localization. The capacity of odorant receptors to recognize odor molecules relies on odorant receptor co-receptors forming heterodimers. Here we report the successful engineering a homozygous mutant strain of diamondback moth (<em>Plutella xylostella</em>) in which the odorant receptor co-receptor <em>PxOrco</em> was silenced using CRISPR/Cas9. This insect is a globally important crop pest for which novel control methods are urgently required. Behavioral assays demonstrated that <em>PxOrco</em> knockout males exhibited abolished courtship behaviors, inability to mate, and loss of selective preference for <em>P. xylostella</em>'s key sex pheromone components. Whilst female mating behavior and fecundity remained unaffected by <em>PxOrco</em> knockout, oviposition response to leaf alcohol, a key cue for normal oviposition behavior, was lost. Electroantennography revealed drastically reduced responses to sex pheromones and plant volatiles in <em>PxOrco</em>-deficient adults but food location by larvae was unaffected. Moreover, expression analysis of <em>PxOrco-</em>deficient pheromone receptors (PRs) indicated varied regulation patterns, with down-regulation observed in several PRs in both sexes. These findings underscore the critical role of <em>PxOrco</em> in regulating multiple olfactory aspects in <em>P. xylostella</em>, including feeding, mating, and host location. Our study identifies the potential of disrupting the <em>Orco</em> gene in this and other pest species to provide novel avenues for future pest control.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104248"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lineage-specific duplication and functional diversification of DOPA-decarboxylase genes in the Gryllidae family, as revealed in Gryllus bimaculatus","authors":"Shintaro Inoue ,&nbsp;Kai Fujie ,&nbsp;Taiki Hamaguchi ,&nbsp;Yoshiyasu Ishimaru ,&nbsp;Katsuyuki Miyawaki ,&nbsp;Akira Takahashi ,&nbsp;Takeshi Nikawa ,&nbsp;Sumihare Noji ,&nbsp;Takahito Watanabe ,&nbsp;Taro Mito","doi":"10.1016/j.ibmb.2024.104246","DOIUrl":"10.1016/j.ibmb.2024.104246","url":null,"abstract":"<div><div>The DOPA-decarboxylase (DDC) gene is crucial for dopamine synthesis and influences various biological functions in insects, including body coloration, behavior, learning, and sleep. However, its evolutionary impact remains largely unexplored. This study reports on the tandem duplication of two <em>bona fide ddc</em> genes (<em>ddc1</em> and <em>ddc2</em>) in the <em>Gryllidae</em> cricket family. We herein investigated the function of <em>ddc1</em> and <em>ddc2</em> using <em>Gryllus bimaculatus</em> (<em>Gb</em>) as a model. Our results revealed that <em>Gb'ddc1</em> was expressed systemically, with its expression being higher immediately after molting compared to the stage following melanin pigmentation. In homozygous knockout mutants of <em>Gb'ddc1</em>, generated via CRISPR/Cas9, reduced body color pigmentation and had translucent cuticles, decreased dopamine levels, and over-accumulated DOPA. These mutants died shortly after hatching, likely due to cuticle defects, underscoring the essential role of dopamine, produced by <em>Gb'ddc1,</em> in melanin synthesis. Conversely, <em>Gb'ddc2</em> expression was confined to the ovary and was not up-regulated after molting. Homozygous knockout mutants of <em>Gb'ddc2</em> exhibited no body color defects, whereas hatchability and embryonic development rates were significantly reduced. Interestingly, dopamine levels in the ovaries were significantly elevated in <em>Gb'ddc2</em> mutants. This suggests that normal ovarian dopamine levels, modulated by <em>Gb'ddc2,</em> are vital for fertility maintenance. The function of <em>Gb'ddc2</em> differs from that of typical <em>ddc</em>, indicating neofunctionalization through evolutionary duplication. Overall, <em>Gb'ddc1</em> and <em>Gb'ddc2</em> have distinct functions, and precise regulation of ovarian dopamine levels using these two <em>ddc</em> genes may have enhanced cricket fertility.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104246"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histone deacetylases synergistically regulate juvenile hormone signaling in the yellow fever mosquito, Aedes aegypti","authors":"Sharath Chandra Gaddelapati ,&nbsp;Subba Reddy Palli","doi":"10.1016/j.ibmb.2024.104256","DOIUrl":"10.1016/j.ibmb.2024.104256","url":null,"abstract":"<div><div>Controlling <em>Aedes aegypti</em> mosquitoes is crucial for managing mosquito-transmitted diseases like dengue, zika, chikungunya, and yellow fever. One of the efficient methods to control mosquitoes is to block their progression from the larval to the adult stage. Juvenile hormones (JH) maintain the larval stage and ensure proper developmental timing for transitioning from larval-pupal-adult stages. Our previous studies showed that histone deacetylases (HDACs) regulate JH signaling and metamorphosis in the red flour beetle <em>Tribolium castaneum</em>. However, the role of HDACs in regulating JH signaling in <em>Ae. aegypti</em> mosquito is unknown. To investigate the role of HDACs in JH signaling, we knockdown each <em>HDAC</em> coding gene in Aag-2 cells derived from <em>Ae. aegypti</em>. Knockdown of <em>HDAC1, HDAC4</em>, and <em>HDAC11</em> increased the expression of the JH primary response gene, <em>Krüppel homolog 1 (Kr-h1)</em>, which represses the larval-pupal metamorphosis. Moreover, the simultaneous knockdown of these three <em>HDACs</em> synergistically increased the <em>Kr-h1</em> promoter activity and its expression, mimicking JH action in inducing <em>Kr-h1</em>. Nevertheless, each HDAC regulates the transcription of different sets of genes, except for a few common genes involved in JH signaling. Furthermore, the knockdown of these <em>HDACs</em> in <em>Ae. aegypti</em> larvae caused different phenotypes apart from delayed pupation: <em>HDAC1</em> knockdown caused larval growth retardation, body shrinkage, and eventual death; <em>HDAC4</em> knockdown led to incomplete head capsule shedding after metamorphosis; and <em>HDAC11</em> knockdown caused higher pupal mortality. Our data demonstrates functional overlap and distinct functions for HDAC1, HDAC4, and HDAC11 in modulating JH signaling, with each HDAC having a unique role in mosquito development.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104256"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annotation of transcription factors, chromatin-associated factors, and basal transcription machinery in the pea aphid, Acyrthosiphon pisum, and development of the ATFdb database, a resource for studies of transcriptional regulation","authors":"Nicolas Parisot ,&nbsp;Mélanie Ribeiro Lopes ,&nbsp;Sergio Peignier ,&nbsp;Patrice Baa-Puyoulet ,&nbsp;Hubert Charles ,&nbsp;Federica Calevro ,&nbsp;Patrick Callaerts","doi":"10.1016/j.ibmb.2024.104217","DOIUrl":"10.1016/j.ibmb.2024.104217","url":null,"abstract":"<div><div>The pea aphid, <em>Acyrthosiphon pisum,</em> is an emerging model system in functional and comparative genomics, in part due to the availability of new genomic approaches and the different sequencing and annotation efforts that the community has dedicated to this important crop pest insect. The pea aphid is also used as a model to study fascinating biological traits of aphids, such as their extensive polyphenisms, their bacteriocyte-confined nutritional symbiosis, or their adaptation to the highly unbalanced diet represented by phloem sap. To get insights into the molecular basis of all these processes, it is important to have an appropriate annotation of transcription factors (TFs), which would enable the reconstruction/inference of gene regulatory networks in aphids. Using the latest version of the <em>A. pisum</em> genome assembly and annotation, which represents the first chromosome-level pea aphid genome, we annotated the complete repertoire of <em>A. pisum</em> TFs and complemented this information by annotating genes encoding chromatin-associated and basal transcription machinery proteins. These annotations were done combining information from the model <em>Drosophila melanogaster</em>, for which we also provide a revisited list of these proteins, and <em>de novo</em> prediction. The comparison between the two model systems allowed the identification of major losses or expansions in each genome, while a deeper analysis was made of ZNF TFs (with certain families expanded in the pea aphid), and the Hox gene cluster (showing reorganization in gene position in the pea aphid compared to <em>D. melanogaster</em>). All annotations are available to the community through the Aphid Transcription Factors database (ATFdb), consolidating the various annotations we generated. ATFdb serves as a valuable resource for gene regulation studies in aphids.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104217"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet influence on male sexual maturation through interplay between insulin signaling and juvenile hormone in insects","authors":"Evan Force ,&nbsp;Claudia Alvarez ,&nbsp;Annabelle Fuentes ,&nbsp;Annick Maria ,&nbsp;Françoise Bozzolan ,&nbsp;Stéphane Debernard","doi":"10.1016/j.ibmb.2024.104252","DOIUrl":"10.1016/j.ibmb.2024.104252","url":null,"abstract":"<div><div>In animals, sexual maturation coincides with the development of sexual behaviors and reproductive system. These developmental events are influenced by diet and governed by endocrine signals. Here, for the first time in insects, we explored functional links between nutrition and juvenile hormone (JH) in the male reproductive physiology through the insulin signaling pathway (ISP) acting as a transducer of nutritional signals. We turned to the male moth <em>Agrotis ipsilon</em> for which sexual maturation, including accessory sex glands (ASGs) development concomitantly with antennal lobes (ALs) maturation for female sex pheromone processing and display of sexual behavior, is known to be JH- and diet-dependent. Indeed, a diet rich in sugars with sodium was previously shown to accelerate sexual maturation, which was achieved from the third day of adult life. In this study, we demonstrated that such a diet raised i) the expression of JH signaling actors (Methoprene-tolerant, Taiman, and Krüppel homolog 1) in ALs and ASGs, ii) the biosynthesis and circulating levels of JH, and iii) the expression of both <em>insulin receptor</em> (<em>InR</em>) and <em>insulin-like peptides</em> (<em>ILPs</em>) in <em>corpora allata</em> (CAs) and brain respectively. Insulin injection raised JH biosynthesis following increased <em>HMG-CoA reductase</em> expression in CAs; opposite effects were induced in InR-deficient males. Thus, we highlighted that promoting effects of a diet composed of sugars with sodium on male sexual maturation results from an early induction of ISP causing an increase in JH biosynthesis followed by a potentiation of JH actions on the development of ASGs and ALs in <em>A. ipsilon</em>.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104252"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seminal fluid proteins in the Queensland fruit fly: Tissue origins, effects of mating and comparative genomics","authors":"Khandaker Asif Ahmed ,&nbsp;Heng Lin Yeap ,&nbsp;Chris W. Coppin ,&nbsp;Jian-Wei Liu ,&nbsp;Gunjan Pandey ,&nbsp;Phillip W. Taylor ,&nbsp;Siu Fai Lee ,&nbsp;John G. Oakeshott","doi":"10.1016/j.ibmb.2024.104247","DOIUrl":"10.1016/j.ibmb.2024.104247","url":null,"abstract":"<div><div>In many insect species, the ability of males to inhibit their mates from remating is an important component of fitness. This ability is also essential for the effective management of insect pests, including tephritid fruit flies, using the Sterile Insect Technique. Here we apply transcriptomics and proteomics to male reproductive tissues before and after mating to characterize components of semen that might mediate remating inhibition in Queensland fruit fly. We found 144 genes whose transcripts were enriched, or proteins expressed, in reproductive tissue and which also varied in amount after mating. Some were associated with testes, accessory glands and ejaculatory apodeme, but those from the ejaculatory apodeme were over-represented compared to those not enriched in reproductive tissue or mating responsive. These included 13 related genes clustered within one Mb on chromosome 5. Functional annotations implicated a broad range of biochemical processes in the genes/proteins enriched in reproductive tissue and mating responsive, with cuticle structure most commonly implicated among the subset of these that were apodeme-enriched and a kinase involved in vitellogenesis implicated for one of the 13 clustered genes. We did not find a homolog of the much studied <em>Drosophila melanogaster</em> Sex Peptide but comparative genomics indicated that some of the tissue-enriched, mating responsive genes/proteins were rapidly evolving in tephritids (including in the Queensland fruit fly lineage), suggesting recent adaptation to new functional niches. Our results provide a set of candidate mediators of remating inhibition for further functional testing.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104247"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β2-tubulin and its promoter in the brown planthopper: A versatile tool for genetic control strategies","authors":"Jing-Xiang Chen ,&nbsp;Chuan-Chuan Zhang ,&nbsp;Jia-Wei Sun ,&nbsp;Yi-Bing Zhang,&nbsp;Min-Shi Luo,&nbsp;Wen-Qing Zhang","doi":"10.1016/j.ibmb.2024.104244","DOIUrl":"10.1016/j.ibmb.2024.104244","url":null,"abstract":"<div><div>At present, the application of CRISPR/Cas9 technology for genetic manipulation in insects is predominantly concentrated on Diptera model species, including <em>Drosophila</em> and mosquitoes. In contrast, non-model insects such as the brown planthoppers (BPH, <em>Nilaparvata lugens</em>), a major insect pest of rice, have received less attention in genetic manipulation due to insufficient tools. Here, the analysis of spatiotemporal expression patterns revealed that <em>β2-tubulin</em> in BPH (<em>NlB2t</em>) was predominantly concentrated in male adults and male testis, exhibiting high expression levels. Knockdown of <em>NlB2t</em> expression by using RNAi resulted in the obstruction of male testis development. Mating between the RNAi-treated males and wild-type females led to a notable reduction in the number of eggs laid and the hatching rate of those eggs by 58.2% and 50.6%, respectively. The longevity of RNAi males significantly increased, and females that had previously mated with RNAi males exhibited a diminished inclination for re-mating with wild-type males. The dual-luciferase reporter assay demonstrated robust promoter activity in the upstream 943 bp of <em>NlB2t</em>, capable of driving Cas9 protein expression <em>in vivo</em> and effectively inducing target gene knockout. These findings elucidated that <em>NlB2t</em> may be a key gene in BPH male testis development and reproduction, as a promising target for sterilization. Its upstream promoter serves as a germline promoter, significantly facilitating the development of genetic control tools based on CRISPR/Cas9 technology in BPH.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"177 ","pages":"Article 104244"},"PeriodicalIF":3.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}