Insect Biochemistry and Molecular Biology最新文献

{"title":"Acquisition and repeated alteration of (TTGGG)n telomeric repeats in Odonata (dragonflies and damselflies)","authors":"Tatsuhiro Gotoh ,&nbsp;Haruka Suzuki ,&nbsp;Minoru Moriyama ,&nbsp;Ryo Futahashi ,&nbsp;Mizuko Osanai-Futahashi","doi":"10.1016/j.ibmb.2025.104353","DOIUrl":"10.1016/j.ibmb.2025.104353","url":null,"abstract":"<div><div>Chromosome ends of most eukaryotes are composed of simple telomeric repeats. For arthropods, TTAGG pentanucleotide repeats, (TTAGG)_n has been considered as the ancestral telomeric repeat. However, in the order Odonata, the earliest diverged group in insects that contains dragonflies and damselflies, (TTAGG)_n signal has been almost undetectable in most examined species. Here, we report the pentanucleotide repeat (TTGGG)_n as the typical telomeric repeat sequence for Odonata (dragonflies and damselflies). Based on genomic information from ten Odonata species, (TTGGG)_n was considered the most likely candidate for telomeric repeat sequences. By fluorescence <em>in situ</em> hybridization (FISH) using 12 Odonata species, clear (TTGGG)_n signals were detected at the chromosome ends in both dragonflies and damselflies. By Southern hybridization using 63 Odonata species, strong (TTGGG)_n signals were detected from the majority of species covering all three suborders of Odonata, indicating that the telomeric repeat of the common ancestor of extant Odonata is (TTGGG)_n. Notably, there were a few Odonata species in which (TTGGG)_n signals were faint or absent, suggesting that the telomeric repeat sequence has repeatedly diverged in Odonata, even within genera such as <em>Sympetrum</em>. We also identified telomerase genes in both dragonflies and damselflies, and in some species, more than two telomerase genes are suggested to be present. Overall, this study demonstrates the ancestral acquisition of novel telomeric repeats and their repeated alteration in Odonata.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104353"},"PeriodicalIF":3.2,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of ISG homologous genes in silkworm and revelation of BmNFIL3 inhibition of BmNPV proliferation through regulation of BmRelish transcription","authors":"Wenxuan Lai ,&nbsp;Junmin Xia ,&nbsp;Shigang Fei ,&nbsp;Minyang Zhou ,&nbsp;Yigui Huang ,&nbsp;Wenjie Luo ,&nbsp;Yibing Kong ,&nbsp;Luc Swevers ,&nbsp;Jingchen Sun ,&nbsp;Min Feng","doi":"10.1016/j.ibmb.2025.104352","DOIUrl":"10.1016/j.ibmb.2025.104352","url":null,"abstract":"<div><div>It is generally believed that no functional antiviral pathway exists in insects, that is homologous to the interferon signaling system. However, we have previously identified an interferon-stimulated gene (ISG) homologous gene, <em>BmCH25H</em>, in the silkworm and revealed that BmCH25H relied on its hydroxylase activity as an antiviral effector. Therefore, we speculate that there may be additional ISG homologous genes in the silkworm genome, some of which may play an antiviral role. In this study, based on knowledge on ISGs in mammals, 135 ISG homologous genes were identified in the silkworm genome using gene homology sequence alignment and conserved domain matching methods. Among these ISG homologous genes in the silkworm, we conducted in-depth research on an important immunological transcription factor, nuclear factor interleukin 3 regulated (NFIL3). Our results found that BmNFIL3 could inhibit the proliferation of Bombyx mori nucleopolyhedrovirus (BmNPV). Furthermore, we confirmed that the NFκ-B-related transcription factor BmRelish was regulated by BmNFIL3 and that its induction after BmNPV infection was mediated by BmNFIL3. More importantly, we demonstrated that BmNFIL3 relied on BmRelish for its anti-BmNPV effects. This study represents the first systematic identification of ISG homologous genes in invertebrates and also constitutes the first report that NFIL3 has antiviral effects in insects. These findings will provide new perspectives for the further understanding of antiviral immunity in insects.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104352"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The iolinid mite Pronematus ubiquitus controls a key tomato pest and pathogen by both predation and induction of specific plant defenses","authors":"Lore Vervaet ,&nbsp;Femke De Graeve ,&nbsp;Marilou Vandenhole ,&nbsp;Dominiek Vangansbeke ,&nbsp;Marcus V.A. Duarte ,&nbsp;Emma Lanoo ,&nbsp;Isolde De Beule ,&nbsp;Felix Wäckers ,&nbsp;Patrick De Clercq ,&nbsp;Thomas Van Leeuwen","doi":"10.1016/j.ibmb.2025.104350","DOIUrl":"10.1016/j.ibmb.2025.104350","url":null,"abstract":"<div><div>Tomato production is persistently challenged by pests such as the tomato russet mite <em>(Aculops lycopersici</em>) and pathogens like tomato powdery mildew (<em>Oidium neolycopersici</em>). Traditionally managed with chemical pesticides, sustainable alternatives are needed. This study evaluates the dual role of the omnivorous predatory mite, <em>Pronematus ubiquitus</em>, in directly suppressing pest populations and pathogen infection and indirectly inducing plant defense responses in tomato. Laboratory and greenhouse experiments were conducted using the standard cultivar Castlemart and its jasmonate-deficient mutant, defenseless-1 (def-1), to disentangle the contributions of direct predation from plant-mediated defenses. Pre-exposure of tomato plants to <em>P. ubiquitus</em> significantly reduced <em>A. lycopersici</em> oviposition on Castlemart but not on def-1 plants, implicating jasmonic acid (JA)-dependent defenses in mediating this effect. In vitro assays further demonstrated that <em>P. ubiquitus</em> feeding delayed spore germination and slowed down the development of powdery mildew. Under greenhouse conditions, prolonged exposure to high densities of <em>P. ubiquitus</em> resulted in a marked reduction in powdery mildew incidence compared to both untreated controls and plants treated with the established defense inducer, <em>Macrolophus pygmaeus</em>. Transcriptomic analyses revealed that infestation by <em>P. ubiquitus</em> triggered extensive reprogramming of defense-related gene expression, including the upregulation of key components involved in JA, salicylic acid, and ethylene signaling pathways, as well as genes associated with secondary metabolite biosynthesis and pathogen recognition. Collectively, these findings demonstrate that <em>P. ubiquitus</em> confers enhanced protection against both <em>A. lycopersici</em> and <em>O. neolycopersici</em> through a combination of direct predation and the elicitation of multifaceted plant defenses, offering promising implications for sustainable pest management in tomato cultivation.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104350"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laodelphax striatellus salivary sheath protein Lsactin triggers defense response in plants","authors":"Liangxuan Qi ,&nbsp;Jing Li ,&nbsp;Han Wang ,&nbsp;Jing Li ,&nbsp;Xinyang Tan ,&nbsp;Zhichang Zhao ,&nbsp;Sicong Zhou ,&nbsp;Jichao Fang ,&nbsp;Rui Ji","doi":"10.1016/j.ibmb.2025.104351","DOIUrl":"10.1016/j.ibmb.2025.104351","url":null,"abstract":"<div><div>Herbivore-associated molecular patterns (HAMPs) in saliva enable plants to detect herbivores and activate pattern-triggered immunity (PTI). Piercing-sucking herbivores secrete gel-like saliva that forms salivary sheaths, which aid in feeding. However, the role of proteins within these sheaths in modulating plant defenses remains poorly understood. In this study, we identified a thermostable HAMP, actin-related protein 1 (Lsactin), from the salivary sheath of the small brown planthopper (SBPH) <em>Laodelphax striatellus</em>. Lsactin is a widely conserved arthropod protein that serves as an inducer of the BAK1-dependent PTI response in <em>Nicotiana benthamiana</em>, thereby enhancing its resistance to both <em>Bemisia tabaci</em> and <em>Spodoptera frugiperda</em>. Moreover, this HAMP can also induce cell death in plants such as eggplant, cotton, pepper, and corn. Plants were able to specifically recognize the Lsactin 41-amino-acid peptide (LP41), which functions as a minimal immunogenic epitope. Furthermore, LP41 stimulates the production of jasmonic acid and hydrogen peroxide in rice, enhancing rice's resistance to the SBPH. However, silencing <em>Lsactin</em> in SBPH led to the disruption of salivary sheath formation, which reduced insect feeding efficiency. This study demonstrates that Lsactin from the SBPH salivary sheath serves as a crucial HAMP, triggering plants resistance to various insect species.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104351"},"PeriodicalIF":3.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic network remodeling through PxJHE modulates temperature adaptation in a cosmopolitan insect","authors":"Gaoke Lei ,&nbsp;Huiling Zhou ,&nbsp;Guangyu Yu ,&nbsp;Xin Miao ,&nbsp;Yu Zhang ,&nbsp;Zongyao Ma ,&nbsp;Yating Duan ,&nbsp;Yanting Chen ,&nbsp;Fengluan Yao ,&nbsp;Liette Vasseur ,&nbsp;Shijun You","doi":"10.1016/j.ibmb.2025.104348","DOIUrl":"10.1016/j.ibmb.2025.104348","url":null,"abstract":"<div><div>Understanding the molecular mechanisms underlying temperature adaptation in agricultural pests is crucial for predicting their evolutionary responses to climate change. Here, we investigate the role of juvenile hormone esterase gene <em>PxJHE</em> in temperature adaptation of <em>Plutella xylostella</em>, a globally distributed pest. Spatial-temporal expression patterns demonstrates significantly reduced <em>PxJHE</em> transcript levels in hot-evolved (HS), and cold-evolved (CS) strains across all tested temperatures compared to ancestral strain (AS). CRISPR/Cas9-mediated knockout strains (JHE-MU) exhibit substantially elevated juvenile hormone (JH) titers during development, accompanied by impaired extreme temperature tolerance and altered life history parameters. Biochemical analyses reveal that PxJHE deficiency leads to significant accumulation of lipids and total sugars, while simultaneously reducing antioxidant enzyme activities (SOD and CAT). Metabolomic profiling reveal that <em>PxJHE</em> deficiency causes extensive metabolic rewiring, particularly in lipid, carbohydrate and amino acid pathways. Gene expression analysis demonstrates that <em>PxJHE</em> knockout downregulates key metabolic enzymes including 6-phosphofructokinase (<em>PxPFK</em>) and hormone-sensitive lipase (<em>PxHSL</em>), indicating impaired energy mobilization despite enhanced substrate storage. Our findings demonstrate that <em>PxJHE</em> modulates temperature adaptation through a multi-level regulatory mechanism involving JH signaling, metabolic network coordination, and antioxidant defense systems. This study provides novel insights into the genetic architecture of climate adaptation in agricultural pests, revealing the crucial interface between hormonal regulation, metabolic plasticity, and oxidative stress management in environmental adaptation.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104348"},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropeptide profiling of Bombus terrestris (Hymenoptera, Apidae) and comparative analysis across hymenopteran species","authors":"Lapo Ragionieri","doi":"10.1016/j.ibmb.2025.104347","DOIUrl":"10.1016/j.ibmb.2025.104347","url":null,"abstract":"<div><div><em>Bombus terrestris</em> is a Palearctic bumblebee species known for its excellent pollination activity. Due to its versatility, it has recently become a preferred species for greenhouse pollination worldwide. This species is also frequently used in experiments aimed at studying both the behavioral and physiological aspects of Hymenoptera. Key molecules in these research fields are neuropeptides, which regulate nearly all physiological processes in metazoans. In the current study, initial sequence identification and mass spectrometry analyses were conducted to identify processed neuropeptides in <em>B. terrestris</em> workers. The list of neuropeptide precursors was updated, and their distribution across the CNS was investigated using a combination of mass spectrometry and immunohistochemistry. Specific features of the <em>B. terrestris</em> peptidome were identified as well as those shared with other Apidae. In <em>B. terrestris</em>, some peptidergic systems were not identified, some of which are also absent in other Hymenoptera, while few neuropeptides, such as sulfakinin and corazonin, show derived sequences. Moreover, using mass spectrometry, for the first time, were identified neuropeptides from the abdominal transverse nerve neurohemal organ of Apidae and the presence of elevenin in the thoracic perisympathetic organs of <em>B. terrestris</em> was also reported. Additionally, a comparison of the <em>B. terrestris capa</em> gene structure with other hymenopteran species revealed a deletion in one exon shared by Apidae + Megachilidae, supporting the monophyly of long-tongued bees.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104347"},"PeriodicalIF":3.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NlAgo3 and NlBiwi are essential for piRNAs biogenesis and female reproduction in Nilaparvata lugens","authors":"Shengli Jing ,&nbsp;Jingang Xu ,&nbsp;Fang Zheng ,&nbsp;Jianfeng Jin ,&nbsp;Xiaoyan Huang ,&nbsp;Libo Mao ,&nbsp;Feifei Wang ,&nbsp;Hanmo Zhang ,&nbsp;Jingwen Xu ,&nbsp;Jing Yang ,&nbsp;Ruixian Chen ,&nbsp;Bin Yu ,&nbsp;Qingsong Liu ,&nbsp;Yanan Li","doi":"10.1016/j.ibmb.2025.104346","DOIUrl":"10.1016/j.ibmb.2025.104346","url":null,"abstract":"<div><div>P-element-induced wimpy testis (PIWI) -interacting RNAs (piRNAs) are short non-coding RNAs crucial for silencing transposable elements (TEs) and maintaining genome stability, particularly in germ cells. While most research on piRNAs and PIWI proteins has traditionally focused on model organisms such as <em>Drosophila</em>, emerging studies are now extending to other insect orders. However, little is known about piRNAs in insects of the Hemiptera order. Here, we investigated the role of piRNAs in the brown planthopper (BPH), <em>Nilaparvata lugens,</em> a significant pest of rice that belongs to the Hemiptera order. We identified and characterized two PIWI subfamily protein genes in <em>N. lugens</em>: <em>NlAgo3</em> (a homolog of Ago3) and <em>NlBiwi</em> (the BPH Piwi). Both proteins contain Piwi/Argonaute/Zwille (PAZ) and PIWI domains. Sequence alignment and phylogenetic analysis demonstrated that NlAgo3 and NlBiwi are conserved across other insect orders. <em>NlAgo3</em> and <em>NlBiwi</em> are highly expressed in female adults and their ovaries in <em>N. lugens.</em> Importantly, knockdown of <em>NlAgo3</em> and <em>NlBiwi</em> significantly impaired eggs laying, leading to female sterility, suggesting that these proteins play a crucial role in female reproduction in <em>N. lugens</em>. Subsequently, through small RNA sequencing, we characterized piRNAs in <em>N. lugens</em> and identified a length peak in the range of 26–28 nucleotides, with differences in piRNA abundance between females and males. Additionally, we observed that piRNA clusters were distributed across each chromosome, with a higher density on the sex chromosomes. Moreover, RNAi-mediated knockdown of <em>NlAgo3</em> and <em>NlBiwi</em> severely impaired piRNA production in BPH, highlighting that these proteins are essential for piRNA biogenesis in <em>N. lugens</em>, consistent with findings in other species. Overall, this study provides valuable insights into the role of piRNAs in the reproductive biology of <em>N. lugens</em>, a major pest of rice. By elucidating the characteristics of piRNAs in this insect species, this research enhances our understanding of piRNA-mediated gene regulation in Hemiptera insects. These findings have potential implications for the development of novel strategies for pest control.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104346"},"PeriodicalIF":3.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the biological characteristics and the synthesis pathway of cantharidin in the family meloidae (Insecta: Coleoptera)","authors":"Qian Sun ,&nbsp;Bing Wang ,&nbsp;Yingying Xie ,&nbsp;Fei Xue ,&nbsp;Shuangcheng Ma ,&nbsp;Bonian Zhao ,&nbsp;Yongqiang Lin","doi":"10.1016/j.ibmb.2025.104345","DOIUrl":"10.1016/j.ibmb.2025.104345","url":null,"abstract":"<div><div>Cantharidin, an active compound with well-established clinical efficacy, is a defensive secondary metabolite primarily derived from insects in the <em>Meloidae</em> family. The study of cantharidin's synthetic pathway is not only one of the most intriguing research areas within the <em>Meloidae</em> family, but also holds promise in bridging the gap between biological defense mechanisms and clinical applicability. This paper provides a concise overview of the cantharidin-related biological characteristics of <em>Meloidae</em> beetles. It then summarizes recent advances in research on the synthetic pathway of cantharidin, focusing on three key areas: the synthesis of sesquiterpene precursors, the production and metabolism of farnesol, and its connection to juvenile hormone metabolism. The hypothesis is proposed that the biosynthetic pathway starts with the mevalonate pathway, which synthesizes farnesol, the identified precursor of cantharidin. Farnesol is subsequently converted by enzymes into farnesoic acid, which is further transformed into juvenile hormone acid. Juvenile hormone acid is then converted into juvenile hormone acid diol, which participates in the synthesis of cantharidin. Finally, we propose a speculative synthesis pathway for cantharidin based on current research findings and discuss its implications.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104345"},"PeriodicalIF":3.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionotropic receptors participate in the perception of sinigrin in Plutella xylostella adults","authors":"Zhi-Qiang Wei ,&nbsp;Ya-Xin Wang ,&nbsp;Ji-Xiang Wang ,&nbsp;Xuan-Pu Luan ,&nbsp;Chen Ding ,&nbsp;Fan-Zheng Meng ,&nbsp;Yu He ,&nbsp;Qi Yan ,&nbsp;Jin Zhang ,&nbsp;Shuang-Lin Dong","doi":"10.1016/j.ibmb.2025.104344","DOIUrl":"10.1016/j.ibmb.2025.104344","url":null,"abstract":"<div><div>Glucosinolates serve as the key gustatory cue for crucifer specialists to select their host plants, however, the molecular mechanisms underlying the gustation remain poorly understood. Here, we demonstrated that two ionotropic receptor co-receptors (Ircos) were involved in the perception of a major glucosinolate, sinigrin, in the adult <em>Plutella xylostella</em>. First, we cloned 13 gustatory receptors (GRs) expressed in adults (antennae and tarsi) and larvae (heads), and analyzed their functions using the <em>Xenopus</em> oocyte expression system. It showed that none of these GRs responded to sinigrin. Then we turned to ionotropic receptors (IRs), particularly the two Ircos (PxylIR25a and PxylIR76b), starting with establishment of two single Irco knockout and one double Ircos knockout insect strains using CRISPR-Cas9 approach. Further behavioral assays revealed that knockout of these two Ircos (in both single and double knockout) significantly reduced the sinigrin perception in female adults with no difference among the three knockout strains, suggesting that the two IRs participate in collaboration in the sinigrin perception. However, larval feeding preference for the sinigrin remained generally unaffected in the three knockout strains. These results demonstrate that adult <em>P. xylostella</em> perceive sinigrin at least partly through IR-mediated pathway, but the larvae employ other pathways. Our findings provide new insights into the gustatory mechanisms of glucosinolates, and provide potential molecular targets for development of new control strategies in <em>P</em>. <em>xylostella</em> and other crucifer specialists.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104344"},"PeriodicalIF":3.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic and transcriptomic analysis of the effects of SRBSDV infection on saliva secretion in WBPH","authors":"Qing-Ling Qi,&nbsp;Xiao-Han Xu,&nbsp;Shiqiang Xu,&nbsp;Jian-Ping Chen,&nbsp;ZongTao Sun,&nbsp;Wei Wu","doi":"10.1016/j.ibmb.2025.104343","DOIUrl":"10.1016/j.ibmb.2025.104343","url":null,"abstract":"<div><div>Most plant viruses rely on insect vectors for transmission, with insect saliva serving as a critical interface in tripartite virus-vector-host interactions. Southern rice black-streaked dwarf virus (SRBSDV), a destructive pathogen transmitted by the white-backed planthopper (<em>Sogatella furcifera</em>, WBPH), severely threatens rice production, yet the mechanisms by which SRBSDV manipulates WBPH salivary secretion to enhance viral spread remain poorly understood. Here, integrated proteomic and transcriptomic analyses revealed that SRBSDV infection significantly reduces salivary protein diversity and abundance in WBPH, including digestive enzymes, redox regulators, and effector proteins critical for suppressing plant defenses. LC-MS/MS-based quantification demonstrated a marked decline in secreted protein types and altered abundance profiles in SRBSDV-infected WBPH compared to SRBSDV-free counterparts. Transcriptomic profiling identified 1803 differentially expressed genes in SRBSDV-infected salivary glands, with transmembrane transport-related pathways being prominently downregulated. Crucially, cross-omics integration revealed minimal correlation between salivary protein secretion and transcriptional changes, suggesting that SRBSDV primarily disrupts salivary function by impairing transmembrane transport capacity rather than directly suppressing protein synthesis. This secretory defect likely attenuates the insect's ability to neutralize plant defenses, driving behavioral shifts toward frequent probing that enhance viral inoculation efficiency. Our study unveils a novel strategy whereby SRBSDV hijacks vector secretory machinery to optimize transmission, advancing understanding of virus-vector coevolution and providing a foundation for effector-targeted interventions to block viral spread.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104343"},"PeriodicalIF":3.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}