Insect Biochemistry and Molecular Biology最新文献_第6页

Identification of a cytochrome P450, CYP6CY6v2, as a cytokinin side-chain hydroxylase from the aphid Acyrthosiphon pisum 蚜虫细胞分裂素侧链羟化酶CYP6CY6v2的鉴定

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-06-02 DOI: 10.1016/j.ibmb.2025.104333

Kei Sato , Zui Fujimoto , Yoshihito Suzuki

引用次数: 0

The cys-loop ligand-gated ion channel gene superfamilies of the Lepidopteran species, Bombyx mori and Chilo suppressalis 鳞翅目、家蚕和家蚕的cys环配体门控离子通道基因超家族。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-30 DOI: 10.1016/j.ibmb.2025.104331

Andrew K. Jones , Zijiao Song , Qiutang Huang , Xin Mao , Zhongqiang Jia , Jie Jiang , Tejeswini Dandu , Chunqing Zhao

{"title":"The cys-loop ligand-gated ion channel gene superfamilies of the Lepidopteran species, Bombyx mori and Chilo suppressalis","authors":"Andrew K. Jones , Zijiao Song , Qiutang Huang , Xin Mao , Zhongqiang Jia , Jie Jiang , Tejeswini Dandu , Chunqing Zhao","doi":"10.1016/j.ibmb.2025.104331","DOIUrl":"10.1016/j.ibmb.2025.104331","url":null,"abstract":"<div><div>The rice stem borer, <em>Chilo suppressalis</em> (Walker), is a major pest of rice crops. It is commonly managed by pesticides, several of which act on cys-loop ligand-gated ion channels (cysLGICs). In insects, the cysLGIC superfamily consists of receptors that mediate fast excitatory or inhibitory synaptic transmission in the nervous system as well as performing non-neuronal roles. We present here the cysLGIC superfamily of <em>C. suppressalis</em> as well as that of the silk worm, <em>Bombyx mori</em> (Linnaeus), the first complete gene families from Lepidopteran species, which consists of 27 and 24 subunit-encoding genes, respectively. As with other insects, <em>Chilo</em> and <em>Bombyx</em> possess ion channels predicted to be gated by acetylcholine, γ-aminobutyric acid, glutamate and histamine, as well as orthologues of the <em>Drosophila</em> pH-sensitive chloride channel, CG8916 and CG12344. Both <em>C. suppressalis</em> and <em>B. mori</em> lack GRD (GABA<sub>A</sub> and glycine receptor-like subunit of <em>Drosophila</em>), instead possessing extra RDL (resistance to dieldrin) subunits. Quantitative polymerase chain reaction analysis showed that the <em>C. suppressalis</em> cysLGIC subunits can show differential expression levels throughout the insect's life cycle. Our findings reveal the complete cysLGIC gene families of <em>B. mori</em> and <em>C. suppressalis</em>. These findings enhance our understanding of the evolution of the insect cysLGIC superfamily and provide a useful basis for studying their function and action of pesticides.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104331"},"PeriodicalIF":3.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144197921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A testis-specific heat shock protein gene plays a critical role in male fertility in the fall armyworm 睾丸特异性热休克蛋白基因在秋粘虫雄性生殖能力中起关键作用

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-28 DOI: 10.1016/j.ibmb.2025.104332

Yuchen Zhao , Pei Wang , Yalan Cheng , Siyu Tian , Junfeng Zuo , Qianguang Fang , Chenxue Ling , Subba Reddy Palli , Xien Chen

{"title":"A testis-specific heat shock protein gene plays a critical role in male fertility in the fall armyworm","authors":"Yuchen Zhao , Pei Wang , Yalan Cheng , Siyu Tian , Junfeng Zuo , Qianguang Fang , Chenxue Ling , Subba Reddy Palli , Xien Chen","doi":"10.1016/j.ibmb.2025.104332","DOIUrl":"10.1016/j.ibmb.2025.104332","url":null,"abstract":"<div><div>Heat shock proteins (Hsps) are critical for stress responses and multiple physiological processes, yet their function in insect reproduction remains underexplored. In this study, we identified 36 Hsp genes in the fall armyworm (<em>Spodoptera frugiperda</em>), of which LOC118269601 (designated as <em>SfHsp68A</em>) exhibited robust testis-specific expression, peaking during pupal testis development. CRISPR/Cas9-mediated knockout of <em>SfHsp68A</em> resulted in recessive male sterility, significantly reduced testis size, sperm counts, and egg hatch rates. Transcriptomic analysis of mutant testes revealed substantial downregulation of genes linked to mitochondrial function, oxidative phosphorylation, energy metabolism, and reactive oxygen species (ROS) metabolism. These results demonstrate that SfHsp68A is essential for male fertility in <em>S. frugiperda,</em> likely by maintaining mitochondrial integrity and metabolic homeostasis during spermatogenesis. These studies reveal that testis-specific Hsps function in male fertility and highlight <em>SfHsp68A</em> as a potential genetic target for developing sterile insect technology, offering an environmentally sustainable strategy for controlling this globally invasive pest.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104332"},"PeriodicalIF":3.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino acid and acylcarnitine profile of Aedes aegypti (L) post-blood feeding, after permethrin exposure, and during the life cycle 埃及伊蚊吸血后、氯菊酯暴露后和生命周期内氨基酸和酰基肉碱谱。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-22 DOI: 10.1016/j.ibmb.2025.104328

Mariana Lizbeth Jiménez-Martínez , Gerardo de Jesús Trujillo-Rodriguez , María de Lourdes Ramírez-Ahuja , Olga Karina Villanueva-Segura , Patricio Adrián Zapata-Morin , Griffith Samuel Lizarraga , Laura E. Martinez-de-Villarreal , Margarita L. Martinez-Fierro , Ivan Delgado-Enciso , Jose Ignacio Gonzalez-Rojas , Adriana E. Flores , Antonio Guzman-Velasco , Iram Pablo Rodriguez-Sanchez

{"title":"Amino acid and acylcarnitine profile of Aedes aegypti (L) post-blood feeding, after permethrin exposure, and during the life cycle","authors":"Mariana Lizbeth Jiménez-Martínez , Gerardo de Jesús Trujillo-Rodriguez , María de Lourdes Ramírez-Ahuja , Olga Karina Villanueva-Segura , Patricio Adrián Zapata-Morin , Griffith Samuel Lizarraga , Laura E. Martinez-de-Villarreal , Margarita L. Martinez-Fierro , Ivan Delgado-Enciso , Jose Ignacio Gonzalez-Rojas , Adriana E. Flores , Antonio Guzman-Velasco , Iram Pablo Rodriguez-Sanchez","doi":"10.1016/j.ibmb.2025.104328","DOIUrl":"10.1016/j.ibmb.2025.104328","url":null,"abstract":"<div><div><em>Aedes aegypti</em>, a major vector for arboviruses, requires effective control strategies due to rising insecticide resistance. This study quantified 12 amino acids and 24 acylcarnitines in two strains of <em>Ae. aegypti</em> (New Orleans, susceptible; Apodaca, resistant) using tandem mass spectrometry (MS/MS) across three approaches: 1) life cycle stages, 2) fasting and post-blood feeding (24, 48, 72 h), and 3) before and after permethrin exposure. In the life cycle, alanine (Ala) was highest in adult males (40.3 %), arginine (Arg) peaked in adult females (18.35 %), and tyrosine (Tyr) reached 14.74 % in larvae. The acylcarnitine C0 (free carnitine) peaked in larvae stage 4 (94.21 %), while C2 (acetyl carnitine) was highest in adult females (47.68 %). Post-blood feeding, Ala peaked at 72 h (43.34 %), proline (Pro) at 48 h (20.63 %), and Arg decreased from 0 h (18.35 %) to 72 h (11.15 %). Acylcarnitines C0 and C2 showed a decline post-feeding, indicating fatty acid mobilization. After permethrin exposure, resistant mosquitoes exhibited higher Ala (51.7 %) and C2 (57.9 %) levels, suggesting increased metabolic activity for detoxification. In contrast, susceptible mosquitoes displayed less variability in amino acid and acylcarnitine levels. These results provide valuable insights into the metabolic adaptations of <em>Ae. aegypti</em>, indicating the potential for targeting amino acid and acylcarnitine pathways to improve vector control strategies, particularly in the context of rising insecticide resistance.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104328"},"PeriodicalIF":3.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of biliverdin as a pigment involved in the seasonal variation of green lacewing body colour and the enzymes involved in its metabolism 绿草体色季节变化中胆绿素及其代谢酶的鉴定。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-20 DOI: 10.1016/j.ibmb.2025.104330

Shion Abe, Yuka Matsubara, Kanako Ogawa, Yuki Nakamura, Satoshi Yamauchi, Hisashi Nishiwaki

{"title":"Identification of biliverdin as a pigment involved in the seasonal variation of green lacewing body colour and the enzymes involved in its metabolism","authors":"Shion Abe, Yuka Matsubara, Kanako Ogawa, Yuki Nakamura, Satoshi Yamauchi, Hisashi Nishiwaki","doi":"10.1016/j.ibmb.2025.104330","DOIUrl":"10.1016/j.ibmb.2025.104330","url":null,"abstract":"<div><div>The green lacewing, <em>Chrysoperla nipponensis</em>, is an insect that undergoes complete metamorphosis, and its body colour changes depending on the developmental stage; adults and larvae are greenish and brownish, respectively. In addition, the body colour of some adults changes to brown in winter, suggesting that environmental factors influence pigment biosynthesis in this insect. However, the underlying colour regulation systems remain unknown. In this study, we purified the pigments that produce the body colour of green adult lacewings and identified biliverdin via MS/MS analyses. We therefore elucidated the mRNA and amino acid sequences of biliverdin-related biosynthetic enzymes, such as haem oxygenase (HO) and biliverdin reductase (BR), in <em>C. nipponensis</em>. By expressing recombinant BR in <em>E. coli</em>, we investigated the ability of BR to metabolize biliverdin. In addition, via RNAi techniques, we examined whether the suppression of these enzymes changed body colour. Finally, we quantified the expression levels of the mRNAs encoding these enzymes and compared those in green adults reared under normal conditions with those in brown adults reared under winter-mimicking conditions. The results suggest that not only biliverdin but also other green pigments contribute to the green body colour of adult lacewings.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104330"},"PeriodicalIF":3.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas9-induced point mutation on the GABA receptor subunit RDL confers high resistance to phenylpyrazole insecticides in the rice planthopper Laodelphax striatellus CRISPR/ cas9诱导的GABA受体亚基RDL点突变使稻褐飞虱对苯吡唑类杀虫剂具有高抗性

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-20 DOI: 10.1016/j.ibmb.2025.104327

Hui-Jie Wu , Jia-Peng Yang , Wen-Jing Ma , Zheng-Hao Li , Hong-Yu Feng , Zhang-Nv Yang , Hai-Jun Xu

{"title":"A CRISPR/Cas9-induced point mutation on the GABA receptor subunit RDL confers high resistance to phenylpyrazole insecticides in the rice planthopper Laodelphax striatellus","authors":"Hui-Jie Wu , Jia-Peng Yang , Wen-Jing Ma , Zheng-Hao Li , Hong-Yu Feng , Zhang-Nv Yang , Hai-Jun Xu","doi":"10.1016/j.ibmb.2025.104327","DOIUrl":"10.1016/j.ibmb.2025.104327","url":null,"abstract":"<div><div>The Delphacidae planthopper <em>Laodelphax striatellus</em> (Order: Hemiptera) is one of the most damaging insect pests of rice crops in Asia. The phenylpyrazole insecticide fipronil was introduced in China in the mid-1990s to control these pests, but its widespread use has led to the development of high levels of resistance. Field sampling coupled with <em>in vitro</em> assays indicated that an A2′N-point mutation in the gamma-aminobutyric acid receptor RDL has been linked to fipronil resistance; however, genetic evidence supporting this association has been lacking. Here, we generated an A2′N-point mutation (<em>LsRDL</em><sup><em>A/N</em></sup>) in <em>L. striatellus</em> using CRISPR/Cas9-mediated homologous recombination. Bioassay revealed that the <em>LsRDL</em><sup><em>A/N</em></sup> mutants conferred a 1211-fold higher resistance to fipronil compared to wild-type planthoppers. Furthermore, these mutants also showed significant resistance (>1000-fold) to ethiprole but not to imidacloprid, demonstrating that the A2′N mutation confers resistance specific to phenylpyrazole insecticides. However, the <em>LsRDL</em><sup><em>A/N</em></sup> mutants displayed reduced fecundity, lower egg hatching rates, and prolonged egg and nymph stages, indicating that fipronil resistance carries a substantial fitness cost. These findings enhance our understanding of the genetic mechanisms underlying phenylpyrazole resistance and provide valuable insights for developing effective resistance management strategies for planthoppers.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104327"},"PeriodicalIF":3.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silkworm mutagenesis using a ribonucleoprotein-based CRISPR/Cas12a system 利用基于核糖核蛋白的CRISPR/Cas12a系统进行家蚕诱变

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-19 DOI: 10.1016/j.ibmb.2025.104329

Yunlong Zou , Aijun Ye , Meixin Dong, Yuhao Zhou, Wentao Wu, Yuxia Tang, Hai Hu, Fangyin Dai, Xiaoling Tong

{"title":"Silkworm mutagenesis using a ribonucleoprotein-based CRISPR/Cas12a system","authors":"Yunlong Zou , Aijun Ye , Meixin Dong, Yuhao Zhou, Wentao Wu, Yuxia Tang, Hai Hu, Fangyin Dai, Xiaoling Tong","doi":"10.1016/j.ibmb.2025.104329","DOIUrl":"10.1016/j.ibmb.2025.104329","url":null,"abstract":"<div><div>The development of highly efficient genome editing tools has revolutionized developmental biology and genetic studies in silkworm. Although methods based on CRISPR/Cas9 are currently popular, the Cas12a system has emerged as a promising option. However, it has not yet been applied to target the silkworm genome <em>in vivo,</em> and its activity in silkworm has not yet been characterized. In this study, we established a ribonucleoprotein-based CRISPR/Cas12a system, and compared it to the CRISPR/Cas9 system using 19 crRNA and 17 sgRNAs to target three different genes <em>in vivo.</em> Although Cas12a generates mutants less efficiently than Cas9, we used it successfully to generate transmissible indels, and demonstrated its application by targeting the <em>FibH</em> and <em>mp</em> genes to produce mutants with the expected phenotypes. We also assessed the influence of temperature (37 °C vs. 25 °C) on Cas12a activity, and demonstrated that the effects are target dependent. In summary, we have established a ribonucleoprotein-based CRISPR/Cas12a system in silkworm that offers a practical alternative to CRISPR/Cas9 and extends the genome editing tool box available for silkworm research.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104329"},"PeriodicalIF":3.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mini-review: Aspects of cuticle formation and structure advanced by studies in Nilaparvata lugens 综述：龙葵角质层形成和结构的研究进展。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-14 DOI: 10.1016/j.ibmb.2025.104326

Chuan-Xi Zhang , Bernard Moussian

{"title":"Mini-review: Aspects of cuticle formation and structure advanced by studies in Nilaparvata lugens","authors":"Chuan-Xi Zhang , Bernard Moussian","doi":"10.1016/j.ibmb.2025.104326","DOIUrl":"10.1016/j.ibmb.2025.104326","url":null,"abstract":"<div><div>A hallmark of insects is their cuticle that protects them against predators and parasites, prevents desiccation and xenobiotic penetration and, finally, serves locomotion as an exoskeleton. In the last two decades, tremendous efforts have yielded a plethora of molecular and histological data that have boosted our understanding of the mechanisms of cuticle formation and function. As the factors involved in cuticle formation and function are to a large extent specific to insects, these data may be used in intelligent pest management strategies.</div><div>The brown planthopper <em>Nilaparvata lugens</em> is a major rice pest in Asia. Cuticle research in this species has been following findings in other insect species recapitulating most collected results. There are, in addition, several important data in <em>N. lugens</em> that advanced our understanding in cuticle biology in insects generally. For instance, it was demonstrated in the brown planthopper that a chitin-binding protein, namely NlugCpr47 is needed for organ shaping. Moreover, based on genetic data, it was hypothesised that cuticular lipids may be organised in layers depending on the size of the molecules. Finally, pore canals, the nanotubes system that is used as a transport route for cuticle lipid deposition, were visualised in 3D with high resolution for the first time in an insect.</div><div>These examples, reviewed in this article, underline the importance of a detailed molecular and histological approach in non-model insects for a better understanding of cuticle biology in general.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"182 ","pages":"Article 104326"},"PeriodicalIF":3.2,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-biased fertility degeneration induced by depletion of an auxiliary piRNA-pathway factor Qin in Bombyx mori 家蚕一种辅助pirna通路因子秦的缺失引起的性别偏性生育退化

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-05 DOI: 10.1016/j.ibmb.2025.104319

Kai Chen , Ling Ye , Ye Yu , Peilin Guo , Anjiang Tan

{"title":"Sex-biased fertility degeneration induced by depletion of an auxiliary piRNA-pathway factor Qin in Bombyx mori","authors":"Kai Chen , Ling Ye , Ye Yu , Peilin Guo , Anjiang Tan","doi":"10.1016/j.ibmb.2025.104319","DOIUrl":"10.1016/j.ibmb.2025.104319","url":null,"abstract":"<div><div>The PIWI-interacting RNA (piRNA) pathway is the major defense system for transposable elements (TEs) silencing in animal gonads, maintaining genomic integrity of germ cells and ensuring proper gametogenesis. An the piRNA-pathway factor, <em>Qin</em>, has been reported to participate in piRNA biogenesis in the lepidopteran model insect, <em>Bombyx mori</em>. Nevertheless, the physiological functions of <em>Qin</em> remain to be characterized. Here we demonstrated that Qin plays important roles in silkworm gonad development of both sexes. <em>BmQin</em> was predominantly expressed in gonads. Immunofluorescent staining revealed that BmQin is localized in the cytoplasm of both germ cells and somatic cells in gonads. Depletion of <em>BmQin</em> via CRISPR/Cas9 system induceed complete sterile in males, and partial sterile in females. Notably, mutants displayed severe defects in gonad development and gametogenesis. RNA-seq analysis revealed that the piRNA pathway was dysregulated in mutant gonads. In addition, apoptosis was significantly enhanced in mutant gonads. Our study revealed the physiological functions of <em>BmQin</em> in silkworm fertility and its auxiliary roles in the piRNA pathway in both male and female gonads.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"181 ","pages":"Article 104319"},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae) 基于外周水平的香兰素识别分子机制研究（鳞翅目：香兰素科）

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-05-01 DOI: 10.1016/j.ibmb.2025.104317

Ting-Ting Yang , Yu Ma , Zheng Dai , Ying Li , Ji-Xiang Wang , Teng-Fei Bai , Merid Negash Getahun , George F.O. Obiero , Shuang-Lin Dong , Jin Zhang , Qi Yan

{"title":"Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae)","authors":"Ting-Ting Yang , Yu Ma , Zheng Dai , Ying Li , Ji-Xiang Wang , Teng-Fei Bai , Merid Negash Getahun , George F.O. Obiero , Shuang-Lin Dong , Jin Zhang , Qi Yan","doi":"10.1016/j.ibmb.2025.104317","DOIUrl":"10.1016/j.ibmb.2025.104317","url":null,"abstract":"<div><div><em>Orthaga achatina</em> (Lepidoptera Pyralidae) is a specialist pest of the camphor tree <em>Cinnamomum camphora</em>. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species. However, the behavioral response of <em>Orthaga achatina</em> to vanillin, a volatile compound emitted by camphor trees, remains unexplored. In this study, we found that vanillin attracts both male and female <em>O. achatina</em> adults. Fluorescence competitive binding assays further revealed that among the five odorant-binding proteins (OBPs) highly expressed in both male and female antennae, OachOBP7 exhibited the most prominent binding affinity with vanillin. Furthermore, by employing the Xenopus oocyte expression and two-electrode voltage clamp recording system (XOE-TEVC) to conduct a functional characterization of 40 ORs, vanillin was the optimal ligand for OachOR7 among all tested ligands. In addition, with the 3D structure modeling and molecular docking techniques, it was revealed that OachOR7 displayed a relatively high binding affinity (−5.5 kcal/mol), and Gln84 and Asn189 were predicted to be key amino acid residues for binding vanillin. Finally, the two amino acids were verified by site-specific mutagenesis followed by XOE-TEVC, showing that the binding ability of OR7 to vanillin was significantly reduced to 9.23 × 10<sup>−2</sup> μM after the mutation of two amino acids. This study demonstrate vanillin's behavioral attraction to <em>O. achatina</em> and reveal its molecular basis, offering new possibilities for targeted pest management using this compound.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"180 ","pages":"Article 104317"},"PeriodicalIF":3.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0