Andrew K. Jones , Zijiao Song , Qiutang Huang , Xin Mao , Zhongqiang Jia , Jie Jiang , Tejeswini Dandu , Chunqing Zhao
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引用次数: 0
Abstract
The rice stem borer, Chilo suppressalis (Walker), is a major pest of rice crops. It is commonly managed by pesticides, several of which act on cys-loop ligand-gated ion channels (cysLGICs). In insects, the cysLGIC superfamily consists of receptors that mediate fast excitatory or inhibitory synaptic transmission in the nervous system as well as performing non-neuronal roles. We present here the cysLGIC superfamily of C. suppressalis as well as that of the silk worm, Bombyx mori (Linnaeus), the first complete gene families from Lepidopteran species, which consists of 27 and 24 subunit-encoding genes, respectively. As with other insects, Chilo and Bombyx possess ion channels predicted to be gated by acetylcholine, γ-aminobutyric acid, glutamate and histamine, as well as orthologues of the Drosophila pH-sensitive chloride channel, CG8916 and CG12344. Both C. suppressalis and B. mori lack GRD (GABAA and glycine receptor-like subunit of Drosophila), instead possessing extra RDL (resistance to dieldrin) subunits. Quantitative polymerase chain reaction analysis showed that the C. suppressalis cysLGIC subunits can show differential expression levels throughout the insect's life cycle. Our findings reveal the complete cysLGIC gene families of B. mori and C. suppressalis. These findings enhance our understanding of the evolution of the insect cysLGIC superfamily and provide a useful basis for studying their function and action of pesticides.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.