Insect Biochemistry and Molecular Biology最新文献_第7页

Determining the type of aerobic/anaerobic metabolism during exercise in Drosophila using incremental loading exercise 使用增量负荷运动确定果蝇运动期间的有氧/无氧代谢类型。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-01 DOI: 10.1016/j.ibmb.2025.104404

Qin Yi , Meng Ding , Jinglin Liu, Xiaoya Wang, Lan Zheng

{"title":"Determining the type of aerobic/anaerobic metabolism during exercise in Drosophila using incremental loading exercise","authors":"Qin Yi , Meng Ding , Jinglin Liu, Xiaoya Wang, Lan Zheng","doi":"10.1016/j.ibmb.2025.104404","DOIUrl":"10.1016/j.ibmb.2025.104404","url":null,"abstract":"<div><div>Currently, <em>Drosophila</em> exercise is only classified as regular or endurance exercise, and there is no aerobic or anaerobic form of exercise, making <em>Drosophila</em> exercise modeling studies limited. Therefore, this study aimed to determine the type of aerobic and anaerobic exercise in <em>Drosophila</em> using Incremental Load exercise. A seven-day-old wild-type <em>Drosophila</em> was used as a study sample for exercise intervention using a <em>Drosophila</em> exercise device.</div><div>This study was designed with six exercise intensities, including 0.31 rev/s (E1), 0.45 rev/s (E2), 0.59 rev/s (E3), 0.71 rev/s (E4), 0.83 rev/s (E5), 0.91 rev/s (E6), and Control in addition <em>Drosophila</em> in all exercise groups performed a one-time 2.5-h acute exercise, and the control group did no exercise intervention. Anaerobic and aerobic metabolic enzymes in <em>Drosophila</em> were assayed immediately after exercise. Additionally, <em>Drosophila</em> exercise types were determined using hemolymph lactate, respiratory quotient, trehalose concentration, and mitochondrial respiration assays.</div><div>The experimental results show that at E2 exercise intensity, <em>Drosophila</em> mainly uses aerobic metabolism, and at E6 exercise intensity, it mainly uses anaerobic metabolism.We exercised <em>Drosophila</em> for 2 weeks at E2 and E6 exercise intensities to examine whether the physiological differences (cardiac function, climbing ability, sleep ability, lifespan, etc.) were consistent with those observed in mammals undergoing long-term aerobic and anaerobic exercise.The results showed that these differences are consistent with mammals.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104404"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and evaluation of promoters for effective expression of fluorescent protein and high performance of the modified hyPBase in Zeugodacus cucurbitae 葫芦鱼荧光蛋白高效表达启动子筛选与评价及改性hyPBase的高效表达。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-22 DOI: 10.1016/j.ibmb.2025.104425

Siya Ma , Lixin Wang , Ying Yang , Zhongshi Zhou , Zizhen Fan , Yan Wu , Fengqin Cao , Xianwu Lin , Rihui Yan

{"title":"Screening and evaluation of promoters for effective expression of fluorescent protein and high performance of the modified hyPBase in Zeugodacus cucurbitae","authors":"Siya Ma , Lixin Wang , Ying Yang , Zhongshi Zhou , Zizhen Fan , Yan Wu , Fengqin Cao , Xianwu Lin , Rihui Yan","doi":"10.1016/j.ibmb.2025.104425","DOIUrl":"10.1016/j.ibmb.2025.104425","url":null,"abstract":"<div><div>The melon fly (<em>Zeugodacus cucurbitae</em>(Coquillett)) is a highly destructive quarantine pest that inflicts significant damage on the agricultural industry. However, the absence of efficient genetic research tools for this pest hinders its research progress and therefore its control. Among these, the selection of an appropriate promoter plays a central role. In this study, the activities of numerous promoter systems were examined by transfecting Drosophila S2 cells and microinjecting <em>Z. cucurbitae</em> embryos. The results reveal that native promoters drive higher expression within their respective species than in others and that the <em>ZcPUb</em> promoter effectively drives the expression of fluorescent proteins in <em>Z. cucurbitae</em>. A hyperactive <em>piggyBac</em> transposase (hyPBase) was also genetically modified to the control of the <em>ZcPUb</em> promoter and it significantly improved the transgenesis efficiency for constructs of different sizes. The temporal and spatial expression patterns of fluorescence regulated by the <em>ZcPUb</em> promoter in transgenic flies suggest that the <em>ZcPUb</em> promoter-controlled fluorescence can be used as a valuable marker for transgenic screening of <em>Z. cucurbitae</em>. These findings not only highlight the <em>ZcPUb</em> promoter as a crucial resource for future investigations in gene functions and developmental processes in <em>Z. cucurbitae</em>, but also emphasize the importance of identifying native promoters for research and practical application of organisms.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104425"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145367175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide analysis of the spider Pardosa pseudoannulata revealed the function of spliceosome components in cold adaptation 对假环蛛的全基因组分析揭示了剪接体成分在冷适应中的功能。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-22 DOI: 10.1016/j.ibmb.2025.104426

Jingjing Li , Na Yu , Yunru Chen , Zewen Liu

{"title":"Genome-wide analysis of the spider Pardosa pseudoannulata revealed the function of spliceosome components in cold adaptation","authors":"Jingjing Li , Na Yu , Yunru Chen , Zewen Liu","doi":"10.1016/j.ibmb.2025.104426","DOIUrl":"10.1016/j.ibmb.2025.104426","url":null,"abstract":"<div><div>Organisms adapt to cold stress in winter with various molecular mechanisms. Alternative splicing (AS) is one of the most extensive mechanisms in response to cold stress in plants, but has been rarely reported in animals. Arthropods are widely distributed on earth, and overwintering poses a challenge for the population growth. Here, we investigated the role of AS in response to cold stress in arthropods using the spider <em>Pardosa pseudoannulata</em> as a model. We found that about 48.7 % of multi-exon genes underwent AS events in overwintering spiders collected from fields, and ∼35.3 % were alternatively spliced in cold-acclimated spiders in laboratory, showing a gradual increase as temperature decreases. Spliceosome components were concurrently regulated by cold stress in both transcription and AS aspects. Knockdown of <em>SMB/B′</em> and <em>SMFa</em> during cold acclimation impaired the regulation on AS of cold-responsive genes and led to reduced cold stress tolerance. SMB/B’ and SMFa improved cold adaptation probably through regulating AS events of genes from 4 enriched pathways (spliceosome, circadian rhythm, circadian rhythm-fly, and RNA degradation), which have been reported important in tolerance to cold stress. Taken together, spliceosome components contribute to cold acclimation by ensuring the adequate splicing patterns of cold-responsive genes in the spider, and enhanced its tolerance to cold stress.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104426"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145367237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single Drosophila Dscam1 genomic locus generates a vast repertoire of circRNAs facilitated by RNA pairing 单个果蝇Dscam1基因组位点通过RNA配对产生大量环状RNA。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-23 DOI: 10.1016/j.ibmb.2025.104423

Zedong Li , Bingbing Xu , Zhechao Wang , Wenjie Zhang , Jiayan Fu , Lili Wu , Nengcheng Bao , Jinpeng Xie , Ru Yan , Haiyang Dong , Yongfeng Jin

{"title":"A single Drosophila Dscam1 genomic locus generates a vast repertoire of circRNAs facilitated by RNA pairing","authors":"Zedong Li , Bingbing Xu , Zhechao Wang , Wenjie Zhang , Jiayan Fu , Lili Wu , Nengcheng Bao , Jinpeng Xie , Ru Yan , Haiyang Dong , Yongfeng Jin","doi":"10.1016/j.ibmb.2025.104423","DOIUrl":"10.1016/j.ibmb.2025.104423","url":null,"abstract":"<div><div><em>Drosophila melanogaster Dscam1</em> gene has the potential to generate 38,016 linear isoforms through mutually exclusive alternative splicing. Here, using a tailored approach, we identified thousands of circRNA isoforms derived from the <em>Dscam1</em> locus, representing the highest number of circRNA isoforms produced by a single gene to date. The extensive organization of alternative circularization of <em>Dscam1</em>s is conserved within <em>Insecta</em>. Notably, the analysis of circRNA isoforms revealed that alternative back-splicing of variable exon 4s and 6s occurs in a proximity-dependent manner. Interestingly, unlike the classical “loop-in” model mediated by RNA pairing between flanking introns, we revealed a “loop-out” mechanism, in which RNA secondary structures between docking sites and selector sequences facilitate the back-splicing of exons located outside the loop. These findings expand the understanding of the mechanism underlying the generation of circRNA and provide new insights into the expression regulation of highly complex gene loci.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104423"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female accessory reproductive glands of Paederus fuscipes serve as a reservoir of symbiotic pederin-producing bacteria 雌性副生殖腺作为共生的产童精细菌的储存库。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-02 DOI: 10.1016/j.ibmb.2025.104408

Xuhao Song , Hui Meng , Tingbang Yang , Yujie Li , Fake Zheng , Xianghui Yan

{"title":"Female accessory reproductive glands of Paederus fuscipes serve as a reservoir of symbiotic pederin-producing bacteria","authors":"Xuhao Song , Hui Meng , Tingbang Yang , Yujie Li , Fake Zheng , Xianghui Yan","doi":"10.1016/j.ibmb.2025.104408","DOIUrl":"10.1016/j.ibmb.2025.104408","url":null,"abstract":"<div><div><em>Paederus fuscipes</em>, an ecologically and medically important species, is known for its blistering toxin pederin in hemolymph. Evidence demonstrates that the toxin is synthesized by the uncultured symbiotic pederin-producing bacteria (PPB) in <em>P. fuscipes</em>, but the biological characteristics of PPB within the beetle host remain poorly characterized. Here, we investigated PPB abundance variations in <em>P. fuscipes</em> across different factors (sexes, life stages, habitats, and organs), along with their colonization sites and metabolic potentials. The findings revealed that the PPB abundance in female <em>P. fuscipes</em> at the level of individuals and tissues exhibited stable colonization patterns, independent of habitat and time changes. Notably, PPB dominated the bacterial community in females (relative abundance ≥66.08 %) and nearly occupied reproductive organs (relative abundance ≥96.31 %). Moreover, our results indicated that PPB were predominantly enriched in the accessory glands of female reproductive organs, which could serve as a reservoir for PPB proliferation. Although PPB were not cultured in this study, metagenomic binning yielded the draft genome of PPB (CheckM completeness = 85.14 %, contamination = 0), in which genes related to pederin biosynthesis were identified. Phylogenetic analyses revealed that PPB formed a sister clade to <em>Pseudomonas aeruginosa</em> rather than nesting within the <em>P. aeruginosa</em> lineage. Metabolic module prediction analysis revealed specific deficiencies in PPB's energy metabolism and amino acid biosynthesis pathways, suggesting limited free-living potential for PPB. Collectively, this study provides insights into PPB biological characteristics within their beetle host and paves the way for biotechnological exploitation related to pederin production.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104408"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila proteasome subunit Rpn8 controls IMD pathway activation via PGRP-SC2 degradation 果蝇蛋白酶体亚基Rpn8通过PGRP-SC2降解控制IMD途径的激活。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-11 DOI: 10.1016/j.ibmb.2025.104422

Mingfei Wu , Umar Ali , Sen Ding , Chuchu Zhang , Yiheng Jin , Xinyan Cao , Muhammad Abdul Rehman Saeed , Yangyang Zhu , Muhammad Usama , Badar Raza , Qingshuang Cai , Shanming Ji

{"title":"Drosophila proteasome subunit Rpn8 controls IMD pathway activation via PGRP-SC2 degradation","authors":"Mingfei Wu , Umar Ali , Sen Ding , Chuchu Zhang , Yiheng Jin , Xinyan Cao , Muhammad Abdul Rehman Saeed , Yangyang Zhu , Muhammad Usama , Badar Raza , Qingshuang Cai , Shanming Ji","doi":"10.1016/j.ibmb.2025.104422","DOIUrl":"10.1016/j.ibmb.2025.104422","url":null,"abstract":"<div><div>The ubiquitin-proteasome system (UPS) is a highly conserved protein degradation pathway in insects, playing crucial roles in cellular homeostasis, signal transduction, and various biological processes. Here, we demonstrate that the <em>Drosophila melanogaster</em> (fruit fly) regulatory particle non-ATPase 8 (Rpn8), a key subunit of the 26 S proteasomal cap, is indispensable for fly survival upon bacterial infections. Through targeted genetic knockdown, we observed that <em>Rpn8</em>-silenced flies exhibited decreased expressions of several antimicrobial peptides upon bacterial challenges, concomitant with impaired bacterial clearances. Further proteomic and biochemical analyses revealed that Rpn8 controls the proteasome-mediated degradation of peptidoglycan recognition protein SC2 (PGRP-SC2), a typical amidase that antagonizes immune deficiency (IMD) signaling. Silencing of <em>PGRP-SC2</em> prevented the functional role of <em>Rpn8</em> in controlling the fly antibacterial immune defense, suggesting that <em>Rpn8</em> mediates <em>Drosophila</em> innate immunity in a <em>PGRP-SC2</em>-dependent manner. Intriguingly, overexpression of <em>Bombyx mori</em> (silkworm) <em>Rpn8</em> rescued the immune defects in <em>Rpn8 RNAi</em> flies after bacterial infections. Collectively, our findings not only establish <em>Rpn8</em> as a novel immune modulator that bridges UPS-mediated protein degradation with innate immune defense in <em>Drosophila</em> but also offer insights into conserved mechanisms of host-pathogen interactions in other insect species.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104422"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apis mellifera multi-omics responses to ectoparasitic mites: unveiling biomarkers for Tropilaelaps mercedesae and Varroa destructor in honeybee hemolymph through hemolymph peptide and proteome analysis using MALDI-MS and LC-ESI-MS/MS 应用MALDI-MS和LC-ESI-MS/MS对蜜蜂血淋巴中Tropilaelaps mercedesae和Varroa destructor进行肽和蛋白质组学分析，揭示蜜蜂血淋巴中Tropilaelaps和Varroa destructor的生物标志物。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-09 DOI: 10.1016/j.ibmb.2025.104416

Patcharin Phokasem , Michel Bocquet , Sébastien N. Voisin , Chainarong Sinpoo , Camille Houdelet , Panuwan Chantawannakul , Terd Disayathanoowat , Philippe Bulet

{"title":"Apis mellifera multi-omics responses to ectoparasitic mites: unveiling biomarkers for Tropilaelaps mercedesae and Varroa destructor in honeybee hemolymph through hemolymph peptide and proteome analysis using MALDI-MS and LC-ESI-MS/MS","authors":"Patcharin Phokasem , Michel Bocquet , Sébastien N. Voisin , Chainarong Sinpoo , Camille Houdelet , Panuwan Chantawannakul , Terd Disayathanoowat , Philippe Bulet","doi":"10.1016/j.ibmb.2025.104416","DOIUrl":"10.1016/j.ibmb.2025.104416","url":null,"abstract":"<div><div><em>Varroa destructor</em> and <em>Tropilaelaps mercedesae</em> are ectoparasites that pose a significant threat to honeybee populations. It is essential to understand their impact to develop effective management strategies. In this study, we examined the relative abundance of proteins and the expression of immune-related genes in <em>A. mellifera</em> parasitized by the two mites using mass spectrometry analysis of hemolymph and transcriptomics of bleeding honeybees. The study revealed the modulation of genes and proteins associated with immune response and stress management. The <em>apidaecin</em> gene was upregulated in <em>Varroa</em>-infested honeybees and downregulated in <em>Tropilaelaps</em>-infested honeybees, a finding that correlated with MALDI-MS data. Furthermore, variations in the abundance of Hexamerin, Odorant Binding Protein 21, Phenoloxidase and Transferrin were observed in <em>Varroa-</em> and <em>Tropilaelaps</em>-infested honeybees. Using proteomics, potential markers associated with immunity, metabolism and stress responses (e.g. high Glx storage protein, alpha-glucosidase, glyoxylate/hydroxypyruvate reductases) were found to be specific to <em>Varroa</em> and <em>Tropilaelaps</em> infestations. Two Deformed Wing Virus (DWV) proteins exhibit higher abundance in the hemolymph of <em>Tropilaelaps-</em>infested honeybees compared to non-infested and <em>Varroa</em>-infested honeybees, suggesting that <em>Tropilaelaps</em> may weaken honeybee immunity, potentially exacerbating viral infections and colony health decline. This indicates that studying host-mite species interactions could lead to a better understanding of honeybee protection.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104416"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the interplay between tanning, desiccation resistance, and calcium metabolism in cockroaches 探讨蟑螂鞣制、抗旱性和钙代谢之间的相互作用

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-25 DOI: 10.1016/j.ibmb.2025.104428

Olga Volovych, Leah Khananashvili, Ariel D. Chipman

{"title":"Exploring the interplay between tanning, desiccation resistance, and calcium metabolism in cockroaches","authors":"Olga Volovych, Leah Khananashvili, Ariel D. Chipman","doi":"10.1016/j.ibmb.2025.104428","DOIUrl":"10.1016/j.ibmb.2025.104428","url":null,"abstract":"<div><div>The successful terrestrial adaptation of insects has been recently suggested to be significantly influenced by a novel cuticle modification mechanism mediated by the Laccase 2 (Lac-2) enzyme, introducing a new hypothesis in insect evolutionary biology. This enzyme enables rapid recovery following exoskeletons shedding during moulting and reduces the reliance on calcium sources. Although most insect species have abandoned mineral reinforcement in favour of Lac-2-driven tanning, some insects continue to use both processes concurrently, with a possible interplay between them. In this study, we investigated Lac-2-mediated tanning in <em>Blattella germanica</em>, a global pest and model organism, with a particular focus on its potential interactions with calcium metabolism. Our results show that cuticular maturation after moulting shows a trend of increasing duration throughout development. Notably, the timing of <em>BgerLac-2</em> expression peak differs between early, middle, and late instar nymphs and does not always coincide with the pre-moult phase. Deficiency of <em>BgerLac-2</em> had a significant impact on survival. However, supplementing the diet with high calcium content reduced the severity of these effects. The desiccation associated with <em>BgerLac-2</em> deficiency altered elemental calcium level in both the haemolymph and the cuticular surface in <em>B. germanica</em> on a high calcium diet. Since the molecular mechanisms underlying insect calcium metabolism are unexplored, we attempted to identify genes related to those involved in crustacean calcification. Among them, <em>BgerDD5</em>-like demonstrated a positive correlation between its expression and desiccation in the context of a calcium-rich diet.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104428"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145425077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A conserved Piezo–mRPL2/Jun axis controls reproduction via mitochondrial bioenergetics 保守的Piezo-mRPL2/Jun轴通过线粒体生物能量学控制生殖。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-10 DOI: 10.1016/j.ibmb.2025.104417

Jiangwen Luo , Yaru Lu , Zhiruo Zhang , Qian Zhang, Yunzhu Shang, Yajie Yuan, Xiaohui Duan, Long Zhang, Yuxia Tang, Hai Hu, Xiaoling Tong, Fangyin Dai

{"title":"A conserved Piezo–mRPL2/Jun axis controls reproduction via mitochondrial bioenergetics","authors":"Jiangwen Luo , Yaru Lu , Zhiruo Zhang , Qian Zhang, Yunzhu Shang, Yajie Yuan, Xiaohui Duan, Long Zhang, Yuxia Tang, Hai Hu, Xiaoling Tong, Fangyin Dai","doi":"10.1016/j.ibmb.2025.104417","DOIUrl":"10.1016/j.ibmb.2025.104417","url":null,"abstract":"<div><div>Piezo channels are essential mechanosensors, but their roles in reproduction remain poorly understood. Here, we identify a critical function for BmPiezo in silkworm fertility and embryonic diapause, mediated through a conserved downstream regulatory axis. CRISPR/Cas9-mediated knockout of BmPiezo resulted in male sterility and loss of embryonic diapause, accompanied by dysregulation of ribosome-associated genes and oxidative phosphorylation, and reduced ATP production. We identified mitochondrial ribosomal protein L2 (BmmRPL2) and transcription factor BmJun as key downstream effectors, required for spermatogenesis and diapause, respectively. Homozygous disruption of either gene caused embryonic lethality. Cross-species expression analysis revealed strong expression of Piezo2 and mRPL2 in early human spermatids. Remarkably, transgenic expression of murine Piezo2 in BmPiezo−/−silkworms reactivated the Piezo–mRPL2/Jun axis, restored ATP levels, and rescued reproductive defects. These findings define a conserved pathway that connects mechanical sensing to mitochondrial bioenergetics in reproductive regulation, advancing our understanding of fertility mechanisms across species.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104417"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145278602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Replication and transcription of eccDNAfib−L, an extrachromosomal circular DNA in the silk gland of silkworm, Bombyx mori 家蚕蚕丝腺染色体外环状DNA eccDNAfib-L的复制和转录。

IF 3.7 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1016/j.ibmb.2025.104424

Xinyu Tong , Chao Lei , Mei Yin , Qunnan Qiu , Xiaolong Hu , Min Zhu , Chengliang Gong

{"title":"Replication and transcription of eccDNAfib−L, an extrachromosomal circular DNA in the silk gland of silkworm, Bombyx mori","authors":"Xinyu Tong , Chao Lei , Mei Yin , Qunnan Qiu , Xiaolong Hu , Min Zhu , Chengliang Gong","doi":"10.1016/j.ibmb.2025.104424","DOIUrl":"10.1016/j.ibmb.2025.104424","url":null,"abstract":"<div><div>Extrachromosomal circular DNA (eccDNA) plays critical roles in gene regulation and genome plasticity. Some eccDNAs, known as microDNAs, are less than 1000 bp in length and are capable of autonomous transcription. However, whether microDNAs can replicate remains unclear. We previously identified an eccDNA, eccDNA<sup>fib−L</sup>, which is 542 bp in size (Chromosome 14: 9,692,083–9,692,624nt) in the silk gland of <em>Bombyx mori</em>. While eccDNA<sup>fib−L</sup> promotes the expression of the <em>fib-</em>L gene, its replication and transcription capabilities remain unknown. In this study, we revealed that eccDNA<sup>fib−L</sup> exists in two forms: double-stranded (eccDNA<sup>fib−L</sup>-ds) and single-stranded forms (eccDNA<sup>fib−L</sup>-ss(+), eccDNA<sup>fib−L</sup>-ss(−)). Both double-stranded and single-stranded eccDNA<sup>fib−L</sup> exhibit replication capability, with its replication mechanism potentially analogous to that of mitochondrial DNA. Furthermore, eccDNA<sup>fib−L</sup>-ds undergoes rolling circle transcription without termination. Transcription of eccDNA<sup>fib−L</sup>-ds utilizes the antisense (−) strand as the template, with the transcription direction consistent with that of the <em>fib-</em>L gene. Structural analysis indicated that the GC nucleotides at position 505–506 nt (with the first nucleotide at the junction site of eccDNA<sup>fib−L</sup>-ds defined as position 1) serve as the transcription start site, which was validated by the absence of transcripts following the mutation of GC to TG/TA. The transcription process of eccDNA<sup>fib−L</sup>-ds is dependent on RNA polymerase Ⅱ and multiple regulatory factors. These findings demonstrate that eccDNA<sup>fib−L</sup> can replicate and transcribe, enhancing our understanding of its role in regulating silk protein synthesis and expanding our knowledge of the functions of eccDNAs.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104424"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145327993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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