Insect Biochemistry and Molecular Biology最新文献

{"title":"Functional analysis of ABCA3 transporters in lipid metabolism regulation of the silkworm, Bombyx mori","authors":"Qingsong Liu ,&nbsp;Xiaoyang Wang ,&nbsp;Hao Chen,&nbsp;Guoli Li,&nbsp;Hongni Li,&nbsp;Ping Zhao,&nbsp;Qingyou Xia","doi":"10.1016/j.ibmb.2025.104421","DOIUrl":"10.1016/j.ibmb.2025.104421","url":null,"abstract":"<div><div>ATP-binding cassette (ABC) transporters form one of the most widespread membrane protein families. They move a wide range of substrates across biological membranes. While members of the ABCA subfamily have been extensively linked to lipid metabolism in mammals, their functions in insects remain unclear. In this study, we investigated the function of <em>Bombyx mori</em> ABCA3 (<em>BmABCA3</em>), a full-length ABCA transporter by bioinformatic analysis, CRISPR/Cas9 knockout, lipid staining, and proteomics analysis. <em>BmABCA3</em> was mainly expressed in metabolically active tissues such as the hemocytes and fat body. Loss of <em>BmABCA3</em> led to growth delay, lower body weight, abnormal integument pigmentation, and increased larval death. Lipid staining showed abnormal lipid buildup in hemocytes and lipid loss in other tissues. Free fatty acid levels were also strongly disrupted. Proteomic analysis revealed changes in fatty acid metabolism, cuticle formation, and vesicle-related transport. Supplementing the diet with cholesterol partly rescued the developmental defects in knockout larvae. These findings show that <em>BmABCA3</em> plays a central role in lipid metabolism and is likely involved in sterol regulation in insects. This study also broadens our understanding of the functional roles of ABC transporters.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104421"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic studies of pheromone-binding proteins (LdisPBP1 and LdisPBP2) from spongy moth Lymantria dispar with natural ligands and their analogues: Enantiomer distinction at external and internal binding sites","authors":"Erika Plettner,&nbsp;Ashna Aulakh ,&nbsp;Mailyn Terrado","doi":"10.1016/j.ibmb.2025.104419","DOIUrl":"10.1016/j.ibmb.2025.104419","url":null,"abstract":"<div><div>Olfaction plays an important role in the reproduction of the spongy moth, <em>Lymantria dispar</em>, a major defoliator of deciduous trees. Detection of the attractant sex pheromone, (7<em>R</em>, 8<em>S</em>)-epoxy-2-methyloctadecane, (+)-disparlure, aids males in the location of mates by triggering upwind flight behavior. The antipode, (−)-disparlure, inhibits upwind flight behavior. The sensory hairs on the antennae of the male moth are innervated by the dendrite of an olfactory neuron, which is immersed in sensory lymph, a solution with pheromone-binding proteins (PBPs). These proteins bind pheromone molecules selectively and are necessary for proper olfactory functioning. Two PBPs from spongy moth are known: <em>LdisPBP1</em> and <em>LdisPBP2</em>. The two proteins exhibit differences in their binding affinities towards various natural and unnatural ligands at their internal binding sites. Ligands are bound by PBPs in a two-step process: ligand capture results in binding to an external site, which is followed by movement of the ligand to the internal site. In this study, we investigate the differences in association and dissociation rates of the two proteins with (+)-disparlure, its enantiomer, (−)-disparlure, and some analogues with heteroatom substitutions of oxygen or sulfur at either carbon-5 or carbon-10, using stopped-flow kinetics. We used a fluorescent reporter (N-phenyl-1-naphthylamine, NPN), which competes with the ligands of interest and reports on external site binding in PBPs. The results are discussed in the context of kinetic selectivity of PBPs towards natural disparlure and synthetic analogue ligands, at their external binding sites, where enantiomer distinction has not been detected previously. Ligand docking simulations at two external sites of A and B forms and at one internal site of the B forms of the two proteins were used to interpret the enantioselectivity in the association and dissociation of the ligands. This paper also discusses the enantiomer selectivity and the molecular role of PBPs in the desorption from hydrophobic surfaces and partition between lipid vesicles and aqueous solutions of the pheromone enantiomers and their heteroatom-substituted analogues. Results are interpreted in terms of the contribution of ligand association and dissociation kinetics to the enantiomer selectivity of the spongy moth PBPs.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104419"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of dsRNA uptake and intracellular trafficking in the fat body of Locusta migratoria","authors":"Lin Yang ,&nbsp;Yanli Wang ,&nbsp;Shuai Li ,&nbsp;Mureed Abbas ,&nbsp;Zhangwu Zhao ,&nbsp;Xuekai Shi ,&nbsp;Kun Yan Zhu ,&nbsp;Jianzhen Zhang","doi":"10.1016/j.ibmb.2025.104432","DOIUrl":"10.1016/j.ibmb.2025.104432","url":null,"abstract":"<div><div>RNA interference (RNAi) is a post-transcriptional gene silencing mechanism initiated by exogenous double-stranded RNA (dsRNA), which leads to the degradation of target mRNA within the cell. Since its discovery, RNAi has become a powerful tool for functional genomics and a promising strategy for pest control. However, the cellular uptake and intracellular trafficking of dsRNA, especially in specific insect tissues, remain poorly understood. In this study, we systematically investigated the uptake, transport, and endosomal escape mechanisms of dsRNA in the fat body of <em>Locusta migratoria</em>. Our results reveal that <em>L. migratoria</em> apolipophorin III (LmApoLp-III), a lipid-binding protein, functions as a key carrier that facilitates the initial transport of dsRNA from the hemolymph to the fat body cell membrane. There, dsRNA is recognized and bound by a set of membrane receptors, including scavenger receptors (LmSRA, LmSRC) and lipoprotein receptors (LmLPR, LmLRP1, LmLRP2, and LmLRP3), forming receptor complexes that mediate cellular internalization via clathrin-mediated endocytosis and macropinocytosis. Intracellular trafficking of dsRNA is orchestrated by several <em>L. migratoria</em> small Rab GTPases (LmRab4, LmRab7, and LmRab9), which are critical for vesicular transport. Furthermore, vacuolar-type H⁺-ATPase (V-ATPase) facilitate the escape of dsRNA from endosomes, allowing its entry into the RNAi machinery to exert its gene-silencing function. Together, these findings provide the first comprehensive characterization of the dsRNA uptake pathway in insect fat body tissue, offering valuable insights into RNAi mechanisms and informing the development of more effective RNAi-based pest control strategies.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104432"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145429902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decaprenyl diphosphate synthases in Monolepta hieroglyphica: DPPS-mediated CoQ biosynthesis impacts juvenile hormone and gut microbiota","authors":"Xuan Song ,&nbsp;Jun-feng Kou ,&nbsp;Chang Liu ,&nbsp;Yong Yang ,&nbsp;Da-wei Zhang ,&nbsp;Chao-qun Yi ,&nbsp;Wen-tao Liu ,&nbsp;Run Chen ,&nbsp;Wei-hao Guo ,&nbsp;Yue-qin Wang ,&nbsp;Tian-tao Zhang ,&nbsp;Yong-jun Zhang","doi":"10.1016/j.ibmb.2025.104431","DOIUrl":"10.1016/j.ibmb.2025.104431","url":null,"abstract":"<div><div>Coenzyme Q (CoQ) is essential for cellular metabolism, however its biosynthesis and function in insects remain largely unexplored. This study investigated decaprenyl diphosphate synthases (DPPSs) in CoQ biosynthesis in the polyphagous leaf beetle <em>Monolepta hieroglyphica</em>, an agricultural pest that acquires CoQ from host plants. Using liquid chromatography-mass spectrometry (LC-MS), CoQ10 (RT = 8.06 min) was identified in soybean, and CoQ9 (RT = 7.17 min) in maize. Notably, both CoQ9 and CoQ10 were detectable in adult <em>M. hieroglyphica</em>. Titration revealed significantly decreased JHIII but increased CoQ9 and CoQ10 levels in maize-fed versus soybean-fed beetles at day 11 (<em>p</em> &lt; 0.0001). Transcriptome analysis revealed that 79.79 % of differentiated expressed genes (DEGs) were upregulated and categorized into five functional groups via KEGG enrichment analysis. Notably, <em>MhieDPPS1</em> expression in maize-fed insects was significantly higher than in soybean-fed insects (<em>p</em> &lt; 0.05), while genes involved in juvenile hormone biosynthesis exhibited significantly reduced expression (<em>p</em> &lt; 0.001). Phylogenetic and sequence analysis confirmed that the DPPS1 and DPPS2 of <em>M. hieroglyphica</em> form a distinct clade separate from vertebrate homologous. Kinetic assays demonstrated that recombine <em>MhieDPPS1</em> catalyzed the synthesis of essential compounds, yielding (<em>E</em>)-GPP at concentrations of 28.42 ± 0.90 μM. RNA interference (RNAi) studies indicated that knockdown of <em>MhieDPPS1</em> and <em>MhieDPPS2</em> resulted in a 29.49 % and 34.06 % reduction in expression, respectively, accompanied by a significant increase in juvenile hormone III levels (27.13 % upregulation, <em>p</em> &lt; 0.05) and reductions in CoQ9 and CoQ10 (suppressed by 8.94 % and 6.64 %, respectively, <em>p</em> &lt; 0.05) and has also been validated through supplementary experiments. Additionally, microbial community analyses revealed significant shifts in gut microbiota composition in transgenic <em>Drosophila melanogaster</em> and <em>M. hieroglyphica</em>, respectively. DPPS manipulation also altered metabolic pathways, lifespan, and gut microbial composition, including increased <em>Wolbachia</em>. Overall, this study elucidates the complex regulatory mechanisms governing isoprenoid metabolism in insects and provides valuable insights into the biosynthetic pathways of coenzymes, potentially informing future research on metabolic functions and evolutionary adaptations.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104431"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145420783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of odorant binding proteins in the response of Tetranychus cinnabarinus to repellent activity of ethyl oleate","authors":"Yi-Juan Chen ,&nbsp;Tong-Yao Zhang ,&nbsp;Nian-Feng Wan ,&nbsp;Jie Zhao ,&nbsp;Jie-Xian Jiang ,&nbsp;Xiang-Yun Ji","doi":"10.1016/j.ibmb.2025.104427","DOIUrl":"10.1016/j.ibmb.2025.104427","url":null,"abstract":"<div><div>Novel pest population control strategies that use natural products to disrupt the host-seeking behaviors of pests are imperative in modern pest management. Odorant-binding proteins (OBPs), which are responsible for odorant recognition and signal transduction in the pest olfactory system, play key roles in host localization. We previously reported that ethyl oleate (EO) exhibits significant repellent properties against the highly destructive polyphagous crop pest <em>Tetranychus cinnabarinus</em>; however, the molecular mechanism remains unknown. In the present study, two OBPs (TcinOBP1 and TcinOBP2) from <em>T. cinnabarinus</em> were identified and their EO-induced expression profiles were detected. The results suggested that <em>TcinOBP1</em> exhibited significant upregulation at 15 min and gradual decrease at 45 min post-EO-treatment. Microscale thermophoresis (MST) showed that the recombinant TcinOBP1 protein displayed a strong binding affinity to EO (Kd = 32.3 μM). RNA interference (RNAi) analysis demonstrated that a significant inhibition of <em>TcinOBP1</em> transcript led to a remarkable reduction in the sensitivity of <em>T. cinnabarinus</em> to the repellent activity of EO. Furthermore, computational simulations indicated that TcinOBP1 employed five α-helices and three disulfide bridges, forming a hydrophobic pocket composed of several nonpolar and polar amino acid residues to bind with EO, and hydrophobic interactions was the dominating driving force. Among these residues, six amino acid residues (Leu83, Trp132, Glu147, Ile148, Met190, and Ile194) were confirmed to be essential for the binding of TcinOBP1 to EO through site-directed mutagenesis and binding assays. These results demonstrated that TcinOBP1 is likely involved in mediating the responses of <em>T</em>. <em>cinnabarinus</em> to EO. This finding provides new insights into the olfactory molecular mechanism of OBPs with chemicals in mites and reveals that TcinOBP1 serves as a novel target for developing new and efficient mite control strategies.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"185 ","pages":"Article 104427"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145425136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-927 links nutrient signals and 20-hydroxyecdysone regulation and mediates oviposition in Bactrocera dorsalis","authors":"Tong-Jun Jin ,&nbsp;Zheng-Yang Wang ,&nbsp;Qian-Ping Xie ,&nbsp;Li-Yuan Zheng ,&nbsp;Guy Smagghe ,&nbsp;Jin-Jun Wang ,&nbsp;Qiang Zhang ,&nbsp;Wei Dou","doi":"10.1016/j.ibmb.2025.104401","DOIUrl":"10.1016/j.ibmb.2025.104401","url":null,"abstract":"<div><div>The interplay between lipid homeostasis and hormonal regulation is critical for reproductive success in female insects. The molecular mechanisms linking lipid metabolism to hormonal pathways, particularly ecdysteroid synthesis, remain poorly understood. In this study, we reveal a novel regulatory role of miR-927 in the oriental fruit fly, <em>Bactrocera dorsalis</em>. <em>U</em>sing <em>in vivo</em> miRNA mimic/inhibitor assays, combined with an <em>in vitro</em> dual-luciferase reporter and RNA pull-down experiments, we demonstrated that miR-927 directly targets <em>BdCYP315a1</em>, a key enzyme in ecdysteroidogenesis, thereby repressing the 20-hydroxyecdysone (20E) levels. RNA interference of <em>BdCYP315a1</em> confirmed its essential role in maintaining 20E homeostasis and proper developmental progression. Further, we show that miR-927 expression is regulated by the insulin/insulin-like signaling (IIS) pathway, linking nutritional status to hormonal control. Changes in miR-927, <em>BdCYP315a1</em>, and 20E levels corresponded with alterations in lipid content, ultimately influencing fecundity. These findings reveal a previously uncharacterized miRNA-mediated network integrating nutritional signals, hormonal regulation, and lipid metabolism to control reproductive outcomes. Our work provides novel insights into the molecular mechanisms underlying insect reproduction, highlights miRNAs as key mediators of hormone-lipid interactions, and offers valuable resources for identifying miRNA targets in non-model insect species.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104401"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cuticular proteins: Essential molecular code for insect survival","authors":"Huitang Qi,&nbsp;Tian Liu","doi":"10.1016/j.ibmb.2025.104402","DOIUrl":"10.1016/j.ibmb.2025.104402","url":null,"abstract":"<div><div>The insect cuticle is essential for survival and environmental adaptation, comprising over 100 cuticular proteins (CPs) that represent approximately 1 % of the insect genome coding genes. This complexity is unique among biological materials, underscoring the diverse roles of CPs in insect biology. However, the reasons for this extensive CP repertoire and their precise functional mechanisms persist as unresolved questions in entomology. Recently, advances in molecular biology and genomics have yielded significant progress in elucidating CP functions. Studies demonstrate that CPs are crucial not only for cuticle formation and development but also for regulating body size, coloration, cuticular structure development, environmental adaptation, immune defense, viral interactions, and insecticide resistance. This review comprehensively summarizes current insect CP research, highlights their diverse physiological and ecological roles, and explores their translational potential for pest management and biomimetic material design.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104402"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the effects of high temperature on autophagy and apoptosis in different organs of Bombyx mori based on calcium ion homeostasis","authors":"Peilin Peng ,&nbsp;Hongbin Zou ,&nbsp;Jialu Cheng ,&nbsp;Haoyi Gu ,&nbsp;Guimin Nong ,&nbsp;Xuelin Qin ,&nbsp;Bing Li","doi":"10.1016/j.ibmb.2025.104384","DOIUrl":"10.1016/j.ibmb.2025.104384","url":null,"abstract":"<div><div>High-temperature stress differentially affects physiological functions in <em>Bombyx mori</em>, including growth and development, silk protein synthesis, and metabolism. To investigate the underlying mechanisms, we explored the mechanisms of autophagy and apoptosis in the midgut, posterior silk gland (PSG), and fat body of <em>B. mori</em> under high-temperature treatment (30 °C and 35 °C), based on calcium ion (Ca²⁺) homeostasis. The results demonstrated that high-temperature treatment significantly reduced the body weight of <em>B. mori</em>. Furthermore, high-temperature treatment could cause damage to the midgut and PSG of <em>B. mori</em>, accompanied by intracellular Ca²⁺ overload, and the transcription levels of autophagy-related genes as well as the expression of LC3-I and LC3-II proteins are both affected. The protein levels of ATG5 and NtATG5 were significantly increased, while the transcription of key apoptotic genes and the protein levels of caspase-3 and cleaved caspase-3 were elevated. Collectively, these results indicated that high temperature induced autophagy in <em>B. mori</em> through Ca²⁺ mediation and promoted its conversion to apoptosis. Additionally, high temperature was demonstrated to inflict minimal damage on the fat body and failed to induce upregulation of autophagy or apoptosis. This study not only unveiled the intricate interplay among Ca²⁺ homeostasis, autophagy, and apoptosis in various organs of <em>B. mori</em> under high-temperature stress, but also revealed differential effects of heat stress across distinct organs. These findings provide a theoretical foundation for the breeding of thermotolerant silkworm varieties.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104384"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly efficient hyPBase-mediated transgenesis facilitates gain-of-function analysis in the cricket Gryllus bimaculatus","authors":"Takahiro Ohde ,&nbsp;Taro Nakamura ,&nbsp;Taro Mito ,&nbsp;Takaaki Daimon","doi":"10.1016/j.ibmb.2025.104405","DOIUrl":"10.1016/j.ibmb.2025.104405","url":null,"abstract":"<div><div>Gain-of-function analysis is critical to directly assessing gene functions but remains challenging in non-traditional model insects. Recent development of the hyperactive <em>piggyBac</em> transposase (hyPBase) has improved the efficiency of germline transformation in ametabolous and holometabolous insects. Here, we report highly efficient somatic and germline transformations mediated by hyPBase in the cricket <em>Gryllus bimaculatus</em>. hyPBase-mediated misexpression of the melanin pathway gene <em>arylalkylamine N-Acetyltransferase</em> (<em>aaNAT</em>) turned the cricket body color from black to a light color, indicating a broad effect on external phenotype in the injected generation. Mosaic expression of the homeotic gene <em>Antennapedia</em> with hyPBase successfully circumvented the potential embryonic lethality, and induced antenna-to-leg homeotic transformation in a hatched nymph. Our data provide a practical methodology for gain-of-function analysis in the hemimetabolous model to understand key evolutionary innovations in insects.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104405"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sub-lethal insecticide stress alters epimutation rate but not genetic mutation rate in the pest insect Myzus persicae","authors":"Benjamin J. Hunt ,&nbsp;Kumar Saurabh Singh ,&nbsp;Bartlomiej J. Troczka ,&nbsp;Joanna Mackisack ,&nbsp;Emma Randall ,&nbsp;Mark Mallott ,&nbsp;Tobias Baril ,&nbsp;James Galbraith ,&nbsp;Bram Kuijper ,&nbsp;Ralf Nauen ,&nbsp;Alex Hayward ,&nbsp;Chris Bass","doi":"10.1016/j.ibmb.2025.104403","DOIUrl":"10.1016/j.ibmb.2025.104403","url":null,"abstract":"<div><div>Insect pests evolve heritable resistance to insecticides through selection acting on genetic variation generated by mutation. It has been hypothesised that exposure to low doses of pesticides may increase mutation rate in pest genomes and accelerate resistance evolution. However, the impact of pesticide-induced stress on the mutation rate of insects has never been empirically tested. Here we leverage new, high-quality genomic resources for the aphid pest <em>Myzus persicae</em> in conjunction with long-term mutation accumulation experiments to interrogate spontaneous genetic and epigenetic mutation rates in insecticide-exposed and unexposed aphid lines. Our data reveal that multigenerational exposure of <em>Myzus persicae</em> to sublethal concentrations of the neonicotinoid insecticide imidacloprid does not increase genetic mutation rate. Rather we show that imidacloprid exposure results in a significantly lower epimutation rate. These findings reveal the rate of origin and composition of (epi)mutations arising in a pest insect and challenge the proposed link between pesticide exposure and the rate of mutation.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"184 ","pages":"Article 104403"},"PeriodicalIF":3.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145135902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}