Insect Biochemistry and Molecular Biology最新文献

{"title":"Relish-mediated C2H2 zinc finger protein IMZF restores Drosophila immune homeostasis via inhibiting the transcription of Imd/Tak1","authors":"Hongjian Zhou ,&nbsp;Li Liu ,&nbsp;Yujia Pang ,&nbsp;Yina Xu ,&nbsp;Jing Wu ,&nbsp;Fei Ma ,&nbsp;Ping Jin ,&nbsp;Xue Zhou","doi":"10.1016/j.ibmb.2024.104138","DOIUrl":"10.1016/j.ibmb.2024.104138","url":null,"abstract":"<div><p>The dysregulation of intensity and duration in innate immunity can result in detrimental effects on the body, emphasizing the crucial need for precise regulation. However, the intricate and accurate nature of innate immunity implies the existence of numerous undiscovered innate immunomodulators, particularly transcription factors. In this study, we have identified a <em>Drosophila</em> C2H2 zinc finger protein <em>CG18262</em>, named Immune-mediated Zinc Finger protein (<em>IMZF</em>), capable of suppressing immune responses of Imd pathway. Mechanistically, <em>IMZF</em> serves as a transcription factor that represses the expression of <em>Imd</em> and <em>Tak1</em>. Intriguingly, our findings also reveal that Relish, an NF-κB transcription factor, positively regulates the expression of <em>IMZF</em>, consequently inhibiting the activation of <em>Imd</em> and <em>Tak1</em> to prevent an exaggerated immune response. Additionally, we have elucidated the pivotal role played by the Relish-<em>IMZF</em>-<em>Imd</em>/<em>Tak1</em> axis in restoring immune homeostasis of <em>Drosophila</em> Imd pathway. In summary, our findings not only unveil a novel C2H2 zinc finger immunoregulatory transcription factor, <em>IMZF</em>, along with its specific mechanism of immune regulation, but also shed light on the dual functionality of Relish in different stages of the immune response by modulating distinct effectors. This discovery provides new insights and enlightenment into the complex regulation of <em>Drosophila</em> innate immunity.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"170 ","pages":"Article 104138"},"PeriodicalIF":3.8,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Androcin 18−1, a novel scorpion-venom peptide, shows a potent antitumor activity against human U87 cells via inducing mitochondrial dysfunction","authors":"Kai Wang ,&nbsp;Tienthanh Nguyen ,&nbsp;Yihan Gao ,&nbsp;Ruiyin Guo ,&nbsp;Chaofan Fan ,&nbsp;Hang Liao ,&nbsp;Jiali Li ,&nbsp;Jinwei Chai ,&nbsp;Xueqing Xu ,&nbsp;Yuxin Gong ,&nbsp;Xin Chen","doi":"10.1016/j.ibmb.2024.104137","DOIUrl":"10.1016/j.ibmb.2024.104137","url":null,"abstract":"<div><p>Scorpion venom is a potent natural source for antitumor drug development due to the multiple action modes of anticancer components. Although the sequence of Androcin 18−1 has been identified from the transcriptome profile of the scorpion venom <em>Androctonus bicolor</em>, its bioactivity remains unclear. In this study, we described the antitumor mechanism whereby Androcin 18−1 inhibits the proliferation and induces apoptosis by inducing cell membrane disruption, ROS accumulation, and mitochondrial dysfunction in human U87 glioblastoma cells. Moreover, Androcin 18−1 could suppress cell migration via the mechanisms associated with cytoskeleton disorganization and MMPs/TIMPs expression regulation. The discovery of this work highlights the potential application of Androcin 18−1 in drug development for glioblastoma treatment.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"170 ","pages":"Article 104137"},"PeriodicalIF":3.8,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140955216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of an agmatine N-acetyltransferase from Bactrocera dorsalis that modulates ovary development","authors":"Fei-yue Teng,&nbsp;Ji-mei Feng,&nbsp;Fu-cai Ma,&nbsp;Zhuo-xin Wang,&nbsp;Yong-yue Lu,&nbsp;Yi-xiang Qi","doi":"10.1016/j.ibmb.2024.104130","DOIUrl":"10.1016/j.ibmb.2024.104130","url":null,"abstract":"<div><p>Agmatine N-acetyltransferase (AgmNAT), which catalyzes the formation of N-acetylagmatine from acetyl-CoA and agmatine, is a member of the GCN5-related N-acetyltransferase family. So far, knowledge of the physiological roles of AgmNAT in insects is limited. Here, we identified one gene encoding protein homologous to that of <em>Drosophila AgmNAT</em> using sequence information from an activity-verified <em>Drosophila AgmNAT</em> in a BLAST search of the <em>Bactrocera dorsalis</em> genome. We expressed and purified <em>B. dorsalis</em> AgmNAT in <em>Escherichia coli</em> and used the purified enzyme to define the substrate specificity for acyl-CoA and amine substrates. Our application of the screening strategy to BdorAgmNAT led to the identification of agmatine as the best amine substrate for this enzyme, with the highest k_cat/K_m value. We successfully obtained a <em>BdorAgmNAT</em> knockout strain based on a wild-type strain (WT) using the CRISPR/Cas9 technique. The ovary development of the <em>BdorAgmNAT</em> knockout mutants was delayed for 10 days compared with the WT specimens. Moreover, mutants had a much smaller mature ovary size and laid far fewer eggs than WT. Loss of function of <em>BdorAgmNAT</em> caused by RNAi with mature WT females did not affect their fecundity. These findings indicate that BdorAgmNAT is critical for oogenesis. Our data provide the first evidence for AgmNAT in regulating ovary development.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"170 ","pages":"Article 104130"},"PeriodicalIF":3.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140907660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deorphanizing an odorant receptor tuned to palm tree volatile esters in the Asian palm weevil sheds light on the mechanisms of palm tree selection","authors":"Binu Antony ,&nbsp;Nicolas Montagné ,&nbsp;Arthur Comte ,&nbsp;Sara Mfarrej ,&nbsp;Jernej Jakše ,&nbsp;Rémi Capoduro ,&nbsp;Rajan Shelke ,&nbsp;Khasim Cali ,&nbsp;Mohammed Ali AlSaleh ,&nbsp;Krishna Persaud ,&nbsp;Arnab Pain ,&nbsp;Emmanuelle Jacquin-Joly","doi":"10.1016/j.ibmb.2024.104129","DOIUrl":"10.1016/j.ibmb.2024.104129","url":null,"abstract":"<div><p>The Asian palm weevil, <em>Rhynchophorus ferrugineus</em>, is a tremendously important agricultural pest primarily adapted to palm trees and causes severe destruction, threatening sustainable palm cultivation worldwide. The host plant selection of this weevil is mainly attributed to the functional specialization of odorant receptors (ORs) that detect palm-derived volatiles. Yet, ligands are known for only two ORs of <em>R. ferrugineus</em>, and we still lack information on the mechanisms of palm tree detection. This study identified a highly expressed antennal <em>R. ferrugineus</em> OR, RferOR2, thanks to newly generated transcriptomic data. The phylogenetic analysis revealed that RferOR2 belongs to the major coleopteran OR group 2A and is closely related to a sister clade containing an <em>R. ferrugineus</em> OR (RferOR41) tuned to the non-host plant volatile and antagonist, α-pinene. Functional characterization of RferOR2 via heterologous expression in <em>Drosophila</em> olfactory neurons revealed that this receptor is tuned to several ecologically relevant palm-emitted odors, most notably ethyl and methyl ester compounds, but not to any of the pheromone compounds tested, including the <em>R. ferrugineus</em> aggregation pheromone. We did not evidence any differential expression of RferOR2 in the antennae of both sexes, suggesting males and females detect these compounds equally. Next, we used the newly identified RferOR2 ligands to demonstrate that including synthetic palm ester volatiles as single compounds and in combinations in pheromone-based mass trapping has a synergistic attractiveness effect to <em>R. ferrugineus</em> aggregation pheromone, resulting in significantly increased weevil catches. Our study identified a key OR from a palm weevil species tuned to several ecologically relevant palm volatiles and represents a significant step forward in understanding the chemosensory mechanisms of host detection in palm weevils. Our study also defines RferOR2 as an essential model for exploring the molecular basis of host detection in other palm weevil species. Finally, our work showed that insect OR deorphanization could aid in identifying novel behaviorally active volatiles that can interfere with weevil host-searching behavior in sustainable pest management applications.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"169 ","pages":"Article 104129"},"PeriodicalIF":3.8,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An upstream signaling gene calmodulin regulates the synthesis of insect wax via activating fatty acid biosynthesis pathway","authors":"Min Li ,&nbsp;Shuo Yan ,&nbsp;Xinying Feng,&nbsp;Qinhong Jiang,&nbsp;Mei Guan,&nbsp;Jie Shen,&nbsp;Zhiqi Liu","doi":"10.1016/j.ibmb.2024.104126","DOIUrl":"https://doi.org/10.1016/j.ibmb.2024.104126","url":null,"abstract":"<div><p>Insect wax accumulates on the surface of insect cuticle, which acts as an important protective barrier against rain, ultraviolet light radiation, pathogens, etc. The waxing behavior, wax composition and molecular mechanism underling wax biosynthesis are unclear in dustywings. Herein, the current study determined the vital developmental stage for waxing behavior in dustywings, examined the components of waxy secretions, and identified key regulatory genes for wax biosynthesis. The wax glands were mainly located on the thorax and abdomen of dustywing adults. The adults spread the waxy secretions over their entire body surface. The metabolomics analysis identified 32 lipids and lipid-like molecules, 15 organic acids and derivatives, 7 benzenoids, etc. as the main components of waxy secretions. The fatty acids represented the largest proportion of the category of lipid and lipid-like molecules. The conjoint analysis of metabolomics and transcriptomics identified two crucial genes <em>fatty acyl-CoA reductase</em> (<em>CsFAR</em>) and <em>calmodulin</em> (<em>CsCaM</em>) for wax biosynthesis. The down-regulation of these genes via nanocarrier-mediated RNA interference technology significantly reduced the amount of wax particles. Notably, the RNAi of <em>CsCaM</em> apparently suppressed the expression of most genes in fatty acid biosynthesis pathway, indicating the <em>CsCaM</em> might act as a main upstream regulator of fatty acid biosynthesis pathway.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"169 ","pages":"Article 104126"},"PeriodicalIF":3.8,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140650151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel target-site mutation (H146Q) outside the ubiquinone binding site of succinate dehydrogenase confers high levels of resistance to cyflumetofen and pyflubumide in Tetranychus urticae","authors":"Emre İnak ,&nbsp;Sander De Rouck ,&nbsp;Berke Demirci ,&nbsp;Wannes Dermauw ,&nbsp;Sven Geibel ,&nbsp;Thomas Van Leeuwen","doi":"10.1016/j.ibmb.2024.104127","DOIUrl":"10.1016/j.ibmb.2024.104127","url":null,"abstract":"<div><p>Mitochondrial electron transfer inhibitors at complex II (METI-II), also referred to as succinate dehydrogenase inhibitors (SDHI), represent a recently developed class of acaricides encompassing cyflumetofen, cyenopyrafen, pyflubumide and cyetpyrafen. Despite their novelty, resistance has already developed in the target pest, <em>Tetranychus urticae</em>. In this study a new mutation, H146Q in a highly conserved region of subunit B of complex II, was identified in a <em>T. urticae</em> population resistant to all METI-IIs. In contrast to previously described mutations, H146Q is located outside the ubiquinone binding site of complex II. Marker-assisted backcrossing of this mutation in a susceptible genetic background validated its association with resistance to cyflumetofen and pyflubumide, but not cyenopyrafen or cyetpyrafen. Biochemical assays and the construction of inhibition curves with isolated mitochondria corroborated this selectivity. In addition, phenotypic effects of H146Q, together with the previously described H258L, were further examined via CRISPR/Cas9 gene editing. Although both mutations were successfully introduced into a susceptible <em>T. urticae</em> population, the H146Q gene editing event was only recovered in individuals already harboring the I260V mutation, known to confer resistance towards cyflumetofen. The combination of H146Q + I260V conferred high resistance levels to all METI-II acaricides with LC₅₀ values over 5000 mg a.i./L for cyflumetofen and pyflubumide. Similarly, the introduction of H258L via gene editing resulted in high resistance levels to all tested acaricides, with extreme LC₅₀ values (&gt;5000 mg a.i./L) for cyenopyrafen and cyetpyrafen, but lower resistance levels for pyflubumide and cyflumetofen. Together, these findings indicate that different mutations result in a different cross-resistance spectrum, probably also reflecting subtle differences in the binding mode of complex II acaricides.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"170 ","pages":"Article 104127"},"PeriodicalIF":3.8,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140772037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics analysis of larval secretions reveals a caste-driven nutritional shift in a social wasp colony","authors":"Levona Bodner ,&nbsp;Weronika Jasinska ,&nbsp;Sofia Bouchebti ,&nbsp;Inon Scharf ,&nbsp;Yariv Brotman ,&nbsp;Eran Levin","doi":"10.1016/j.ibmb.2024.104128","DOIUrl":"10.1016/j.ibmb.2024.104128","url":null,"abstract":"<div><p>Social wasps exhibit a unique nutritional cycle in which adults feed larvae with prey, and larvae provide adults with larval secretions (LS). LS serves as a vital nutritional source for adults, contributing to the colony's health and reproductive success. The LS nutrient composition has been previously reported in various wasp species, yet these analyses focused solely on worker-destined larvae, overlooking the potential caste designation effects on LS composition.</p><p>Using metabolomics techniques, we analysed and compared the metabolite and nutrient composition in LS of queen- and worker-destined larvae of the Oriental hornet. We found that queen-destined LS (QLS) contain greater amounts of most metabolites, including amino acids, and smaller amounts of sugars compared to worker-destined LS (WLS). The amino acid-to-sugar ratio in QLS was approximately tenfold higher than in WLS. Thus, as the colony transitions from the production of workers to the production of reproductives, it gradually experiences a nutritional shift that may influence the behaviour and physiology of the adult nest population. This caste-specific metabolite profile and nutrient composition of LS reflect the differences in the diet and physiological requirements of worker- and queen-destined larvae and may play a critical role in caste determination in social wasps.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"169 ","pages":"Article 104128"},"PeriodicalIF":3.8,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140756077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex","authors":"Lin-Bao Zhu ,&nbsp;Han-Dan Zhu ,&nbsp;Zhi-Hao Huang ,&nbsp;Hui-Hua Cao ,&nbsp;Sadaf Ayaz ,&nbsp;Jia-Yue Yang ,&nbsp;Xi-Ya Chen ,&nbsp;Ying Zhang ,&nbsp;Shi-Huo Liu ,&nbsp;Jia-Ping Xu","doi":"10.1016/j.ibmb.2024.104125","DOIUrl":"https://doi.org/10.1016/j.ibmb.2024.104125","url":null,"abstract":"<div><p>Voltage-dependent anion channel 2 (VDAC2) is an important channel protein that plays a crucial role in the host response to viral infection. The receptor for activated C kinase 1 (RACK1) is also a key host factor involved in viral replication. Our previous research revealed that <em>Bombyx mori</em> VDAC2 (BmVDAC2) and <em>B. mori</em> RACK1 (BmRACK1) may interact with Bombyx mori nucleopolyhedrovirus (BmNPV), though the specific molecular mechanism remains unclear. In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome <em>c</em> (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited cyto c release during mitochondrial apoptosis in a RACK1 and VDAC2-dependent manner. Even the mutant p35, which loses Caspase inhibitory activity, could still bind to VDAC2 and inhibit cyto c release. In summary, our results indicated that BmNPV p35 interacts with the VDAC2-RACK1 complex to regulate apoptosis by inhibiting cyto c release. These findings confirm the interaction between BmVDAC2 and BmRACK1, the interaction between p35 and the VDAC2-RACK1 complex, and a novel target that BmNPV p35 regulates apoptosis in <em>Bombyx mori</em> via interaction with the BmVDAC2-BmRACK1 complex. The result provide an initial exploration of the function of this interaction in the BmNPV-induced mitochondrial apoptosis pathway.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"169 ","pages":"Article 104125"},"PeriodicalIF":3.8,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140604369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion","authors":"Ming-Der Lin ,&nbsp;Chia-Hsien Chuang ,&nbsp;Chih-Hsin Kao ,&nbsp;Shu-Hwa Chen ,&nbsp;Szu-Chieh Wang ,&nbsp;Ping-Heng Hsieh ,&nbsp;Guan-Yu Chen ,&nbsp;Chun-Chia Mao ,&nbsp;Jeng-Yi Li ,&nbsp;Mei-Yeh Jade Lu ,&nbsp;Chung-Yen Lin","doi":"10.1016/j.ibmb.2024.104115","DOIUrl":"https://doi.org/10.1016/j.ibmb.2024.104115","url":null,"abstract":"<div><p>Biting midges, notably those within the Ceratopogonidae family, have long been recognized for their epidemiological significance, both as nuisances and vectors for disease transmission in vertebrates. Despite their impact, genomic insights into these insects, particularly beyond the <em>Culicoides</em> genus, remain limited. In this study, we assembled the <em>Forcipomyia taiwana</em> (Shiraki) genome, comprising 113 scaffolds covering 130.4 Mbps—with the longest scaffold reaching 7.6 Mbps and an N50 value of 2.6 Mbps—marking a pivotal advancement in understanding the genetic architecture of ceratopogonid biting midges. Phylogenomic analyses reveal a shared ancestry between <em>F. taiwana</em> and <em>Culicoides sonorensis</em> Wirth &amp; Jones, dating back approximately 124 million years, and highlight a dynamic history of gene family expansions and contractions within the Ceratopogonidae family. Notably, a substantial expansion of the <em>odorant receptor</em> (<em>OR</em>) gene family was observed, which is crucial for the chemosensory capabilities that govern biting midges' interactions with their environment, including host seeking and oviposition behaviors. The distribution of <em>OR</em> genes across the <em>F. taiwana</em> genome displays notable clusters on scaffolds, indicating localized tandem gene duplication events. Additionally, several collinear regions were identified, hinting at segmental duplications, inversions, and translocations, contributing to the olfactory system's evolutionary complexity. Among the 156 ORs identified in <em>F. taiwana</em>, 134 are biting midge-specific ORs, distributed across three distinct clades, each exhibiting unique motif features that distinguish them from the others. Through weighted gene co-expression network analysis, we correlated distinct gene modules with sex and reproductive status, laying the groundwork for future investigations into the interplay between gene expression and adaptive behaviors in <em>F. taiwana</em>. In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"168 ","pages":"Article 104115"},"PeriodicalIF":3.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140347839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the larval precursor configuration of the Drosophila adult hindgut by G-TRACE analysis","authors":"Xubo Zhang ,&nbsp;Yi Wang ,&nbsp;Lihua Wang ,&nbsp;Yue Zhang ,&nbsp;Xiaoyu Xing ,&nbsp;Zhangwu Zhao ,&nbsp;Wei Dong ,&nbsp;Bernard Moussian ,&nbsp;Jianzhen Zhang","doi":"10.1016/j.ibmb.2024.104114","DOIUrl":"10.1016/j.ibmb.2024.104114","url":null,"abstract":"<div><p>The <em>Drosophila</em> hindgut is a classical model to study organogenesis. The adult hindgut originates from the precursor cells in the larval hindgut. However, the territory of these cells has still not been well determined. A ring of <em>wingless</em> (<em>wg</em>)-expressing cells lies at the anterior zone of both the larval and adult hindgut. The larval Wg ring was thought as a portion of precursor of the adult hindgut. By applying a cell lineage tracing tool (G-TRACE), we demonstrate that larval <em>wg</em>-expressing cells have no cell lineage contribution to the adult hindgut. Additionally, adult Wg ring cells do not divide and move posteriorly to replenish the hindgut tissue. Instead, we determine that the precursors of the adult pylorus and ileum are situated in the <em>cubitus interruptus</em> (<em>ci</em>)-expressing cells in the anterior zone, and deduce that the precursor stem cells of the adult rectum locate in the trunk region of the larval pylorus including <em>hedgehog</em> (<em>hh</em>)-expressing cells. Together, this research advances our understanding of cell lineage origins and the development of the <em>Drosophila</em> hindgut.</p></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"168 ","pages":"Article 104114"},"PeriodicalIF":3.8,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}