Insect Biochemistry and Molecular Biology最新文献_第8页

Nutrition influences immunity: Diet and host-parasite interactions 营养影响免疫力：饮食与宿主-寄生虫之间的相互作用

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-11-06 DOI: 10.1016/j.ibmb.2024.104210

Ioannis Eleftherianos , Wei Zhang , Gianluca Tettamanti , Lillia Daley , Amr Mohamed , David Stanley

引用次数: 0

Notch signaling regulates limb regeneration through Hes1 and HeyL in the Chinese mitten crab Notch信号通过Hes1和HeyL调控中华绒螯蟹的肢体再生

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-30 DOI: 10.1016/j.ibmb.2024.104209

Ju Li , Xiaoyan Lv , Xin Zhang , Xiumei Zhao , Yuxuan Meng , Sidi Liu , Simiao Fu , Jinsheng Sun

{"title":"Notch signaling regulates limb regeneration through Hes1 and HeyL in the Chinese mitten crab","authors":"Ju Li , Xiaoyan Lv , Xin Zhang , Xiumei Zhao , Yuxuan Meng , Sidi Liu , Simiao Fu , Jinsheng Sun","doi":"10.1016/j.ibmb.2024.104209","DOIUrl":"10.1016/j.ibmb.2024.104209","url":null,"abstract":"<div><div>Tissue regeneration is an efficient strategy developed by animals to compensate for damaged tissues, involving various types of progenitor cells. Deciphering the signal network that modulates the activity of these progenitors during regeneration is crucial for understanding the differences in regenerative capacities across species. In this study, we evaluated the expression profile and phenotypic function of Notch signaling during limb regeneration in arthropod Chinese mitten crabs. The expression of key components of the Notch signaling pathway was upregulated at 7-day post-autotomy (7 DPA), and declined later at 18-day post-autotomy (18 DPA). To assess the role of Notch, we injected dsRNA targeting the Notch gene into the automized area and evaluated the regeneration efficiency. Our results indicated that blocking Notch signaling led to regenerative defects, manifested by delays in the wound closure and blastema emergence processes. Furthermore, the expression of Notch target genes, <em>Hes1</em> and <em>HeyL</em>, was significantly reduced following Notch knockdown by dsRNA. Knockdown of <em>Hes1</em> specifically impaired the proliferation and expression of neural progenitor cell markers, without affecting myogenic cells. In contrast, blockage of <em>HeyL</em> inhibited the proliferation and expression of markers in both activated neurogenic and myogenic progenitor cells, while up-regulating markers of quiescent neural progenitor cells. These findings suggest that Notch signaling plays an important role in limb regeneration of <em>E. sinensis</em> by activating downstream effectors Hes1 and HeyL, regulating neurogenesis and myogenesis through distinct mechanisms.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104209"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockout of two uridine diphosphate-glycosyltransferase genes increases the susceptibility of Spodoptera litura to Bacillus thuringiensis toxins 敲除两个尿苷二磷酸-糖基转移酶基因会增加斑蝶对苏云金芽孢杆菌毒素的敏感性。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-28 DOI: 10.1016/j.ibmb.2024.104208

Ensi Shao , Can Wang , Wenhui Zheng , Yige Ma , Shanshan Wang , Li Sha , Xiong Guan , Zhipeng Huang

{"title":"Knockout of two uridine diphosphate-glycosyltransferase genes increases the susceptibility of Spodoptera litura to Bacillus thuringiensis toxins","authors":"Ensi Shao , Can Wang , Wenhui Zheng , Yige Ma , Shanshan Wang , Li Sha , Xiong Guan , Zhipeng Huang","doi":"10.1016/j.ibmb.2024.104208","DOIUrl":"10.1016/j.ibmb.2024.104208","url":null,"abstract":"<div><div>Uridine diphosphate-glycosyltransferases (UGTs) catalyze sugar conjugation of endogenous and exogenous molecules in insects. In this study, 45 putative UGT genes in 11 families were identified from the genome of <em>S. litura</em>. Exposure to Bt toxins in 5th-instar larvae of the WT strain led to a significant upregulation of midgut UGT40 expression, particularly of SlUGT40D20, SlUGT40D22, and SlUGT40F25. This upregulation was not observed following exposure to chemical pesticides. Knockout of the UGT genes <em>SlUGT40D20</em> and <em>SlUGT40D22</em> in <em>S. litura</em> (mutant strains SlUGT40D20-KO and SlUGT40D22-KO) via CRISPR/Cas9-mediated mutagenesis increased susceptibility of <em>S. litura</em> to <em>Bacillus thuringiensis</em> (Bt) insecticidal proteins. However, in comparison with the wild-type (WT) strain, the mutants did not change susceptibility to chemical pesticides. Observations of 5th-instar larval midgut by electron microscopy revealed severe damage to the midgut epithelium caused by Cry1Ac toxin at 10 μg/g in the SlUGT40D20-KO strain compared to the WT. SDS-PAGE and LC MS/MS analyses identified a specific protein band corresponding to putative proteoglycans in the peritrophic matrix of the WT strain, which was absent in the SlUGT40D20-KO strain. Our study suggests an inverse correlation between expression of some UGTs and the susceptibility of <em>S. litura</em> larvae to some Bt toxins.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104208"},"PeriodicalIF":3.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male-specific lethal-3 gene is critical for survival and fecundity in rice brown planthopper, Nilaparvata lugens 雄性特异性致死-3 基因对水稻褐飞虱的生存和繁殖至关重要

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-26 DOI: 10.1016/j.ibmb.2024.104207

Shengli Jing , Jingang Xu , Bingbing Yu , Feifei Wang , Aobo Ren , Fang Zheng , Ruixian Chen , Bin Yu , Hengmin Tang , Qingsong Liu

{"title":"Male-specific lethal-3 gene is critical for survival and fecundity in rice brown planthopper, Nilaparvata lugens","authors":"Shengli Jing , Jingang Xu , Bingbing Yu , Feifei Wang , Aobo Ren , Fang Zheng , Ruixian Chen , Bin Yu , Hengmin Tang , Qingsong Liu","doi":"10.1016/j.ibmb.2024.104207","DOIUrl":"10.1016/j.ibmb.2024.104207","url":null,"abstract":"<div><div>Male-specific lethal-3 (MSL3) is a component of the dosage compensation complex in <em>Drosophila melanogaster</em>, where its mutation leads to male-specific lethality. However, the function of <em>MSL3</em> in hemipteran insects remains unclear. This study investigated the role of the MSL3 homolog in a major rice pest, the brown planthopper (<em>Nilaparvata lugens</em>). We cloned and characterized the gene <em>NlMSL3</em> from <em>N. lugens</em>, which is 1467-bp long and encodes a protein of 488 amino acids. Phylogenetic analysis revealed that MSL3 is conserved across various insect orders, with high conservation in the chromo-barrel domain. Quantitative real-time polymerase chain reaction indicated differential expression levels of <em>NlMSL3</em> between male and female insects during development, with the highest expression in the testes. RNA interference-mediated knockdown of <em>NlMSL3</em> in <em>N. lugens</em> resulted in significant mortality in later instar nymphs and adults compared with the control group. In females, <em>NlMSL3</em> knockdown impaired feeding behavior, leading to decreased body weight, notably reduced honeydew excretion, flat abdomens, decreased vitellogenin expression, and defective ovarian development. When ds<em>NlMSL3-</em>treated males were mated with control females, the number of eggs laid was similar to that laid by the females mated with control males; however, none of the eggs laid by the former hatched into nymphs. These results highlight the crucial role of <em>NlMSL3</em> in the development and fecundity of <em>N. lugens</em>.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104207"},"PeriodicalIF":3.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142538331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional analysis of gut dsRNases in the beet armyworm Spodoptera exigua 甜菜夜蛾肠道 dsRNase 的鉴定和功能分析

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-23 DOI: 10.1016/j.ibmb.2024.104206

Jiangjie Li, Yan Shi, Qi Xue, Guy Smagghe, Kristof De Schutter, Clauvis Nji Tizi Taning

{"title":"Identification and functional analysis of gut dsRNases in the beet armyworm Spodoptera exigua","authors":"Jiangjie Li, Yan Shi, Qi Xue, Guy Smagghe, Kristof De Schutter, Clauvis Nji Tizi Taning","doi":"10.1016/j.ibmb.2024.104206","DOIUrl":"10.1016/j.ibmb.2024.104206","url":null,"abstract":"<div><div>RNA interference (RNAi)-based products have the potential to significantly contribute to insect pest control. However, RNAi efficiency varies widely among different insect orders, particularly in Lepidoptera, where it is often low. One key factor affecting RNAi efficiency is the presence of double-stranded ribonuclease (dsRNase) in the digestive tract, which can degrade dsRNA prior to uptake by gut cells. In this study, four dsRNases were identified in the beet armyworm, <em>Spdoptera exigua</em>, of which two were highly expressed gut dsRNases, <em>SedsRNase1</em> and <em>SedsRNase2</em>. To assess their effect on dsRNA degradation activity via the oral route, CRISPR/Cas9-based gene editing was employed to knock out these gut dsRNases. The results indicate that all mutant strains, including <em>SeKO1</em> (knockout <em>SedsRNase1</em>), <em>SeKO2</em> (knockout <em>SedsRNase2</em>), and <em>SeKO1KO2</em> (knockout <em>SedsRNase1</em> and <em>SedsRNase2</em>), showed significantly decreased dsRNA degradation activity, particularly in the <em>SeKO1KO2</em> mutant strain, where the weakest degradation occurred in both the gut and whole body. Additionally, we noticed that the lack of gut <em>SedsRNases</em> led to a slight extended developmental period and reduced reproductive capacity in <em>S. exigua</em>. Collectively, these findings deepen our understanding of gut <em>SedsRNases</em> and how they can impact the biology of the beet armyworm and can support the exploration dsRNA-based approaches for pest control.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104206"},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The fate of a Solanum steroidal alkaloid toxin in the cabbage looper (Trichoplusia ni) 一种茄科甾体生物碱毒素在菜青虫（Trichoplusia ni）体内的命运。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-23 DOI: 10.1016/j.ibmb.2024.104205

Tobias Ziemke, Ping Wang, Christophe Duplais

{"title":"The fate of a Solanum steroidal alkaloid toxin in the cabbage looper (Trichoplusia ni)","authors":"Tobias Ziemke, Ping Wang, Christophe Duplais","doi":"10.1016/j.ibmb.2024.104205","DOIUrl":"10.1016/j.ibmb.2024.104205","url":null,"abstract":"<div><div>Plants produce complex chemical defenses against herbivores, resulting in the emergence of detoxification strategies in phytophagous insects. While enzymatic detoxification and target site mutagenesis are well-documented, the quantitative contribution of excretion remains less studied. We focus on the cabbage looper (<em>Trichoplusia ni</em>), a generalist herbivore, to elucidate the detoxification of a steroidal alkaloid, solanidine, produced in potato (<em>Solanum tuberosum</em>). Through larval feeding experiments and chemical analysis of metabolites using high-resolution mass spectrometry, we identify solanidine 3-O-β-glucopyranoside and solanidine 3-phosphate as major metabolization products of solanidine. Glycosylation and phosphorylation reactions have not previously been observed in cabbage looper. Modified solanidine derivatives exhibit reduced lipophilicity, preventing passive transport as predicted by physicochemical analyses, and only solanidine was detected in body tissue. In addition, the metabolism of solanidine in a <em>T. ni</em> mutant strain with midgut cadherin protein knocked out was also investigated to examine the potential role of the cadherin, an important receptor for Bt toxins, in steroidal alkaloid detoxification. <em>T. ni</em> cadherin-knockout strain showed lower solanidine conversion (33.9% ± 2.2) and uptake (27.41 ± 0.49 nmol/g) compared to the wild-type strain (51.3% ± 4.1, 33.66 ± 2.48 nmol/g) but similar excretion kinetics. Although solanidine negatively impacted the feeding performance of both strains the cadherin-knockout does not affect the feeding performance. Our study expands the metabolization enzyme repertoire in cabbage loopers, emphasizing the complexity of detoxification mechanisms in generalist herbivores.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104205"},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas13a-mediated visual detection: A rapid and robust method for early detection of Nosema bombycis in silkworms CRISPR/Cas13a 介导的视觉检测：一种早期检测家蚕诺斯玛病的快速而可靠的方法。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-20 DOI: 10.1016/j.ibmb.2024.104203

Yi-Xiang Wu , Samreen Sadiq , Xin-Hao Jiao , Xue-Min Zhou , Lu-Lai Wang , Xin-Ran Xie , Iltaf Khan , Ping Wu

{"title":"CRISPR/Cas13a-mediated visual detection: A rapid and robust method for early detection of Nosema bombycis in silkworms","authors":"Yi-Xiang Wu , Samreen Sadiq , Xin-Hao Jiao , Xue-Min Zhou , Lu-Lai Wang , Xin-Ran Xie , Iltaf Khan , Ping Wu","doi":"10.1016/j.ibmb.2024.104203","DOIUrl":"10.1016/j.ibmb.2024.104203","url":null,"abstract":"<div><div>The sericulture industry faces a significant threat from the Pebrine disease of silkworms, caused by <em>Nosema bombycis</em>. Nonetheless, the current microscopic diagnostic methods can be time-consuming, labor-intensive, and lacking sensitivity and accuracy. Therefore, it is crucial to develop a novel detection approach that is efficient, highly sensitive, and low-cost. In this regard, the CRISPR/Cas system has the potential to be a fast, accurate, and highly specific method of detection. Herein, using a microplate reader, a portable fluorescence detection device, and test strips as signal output tools respectively, we have efficiently developed three rapid and facile visual detection methods for <em>N. bombycis</em> using a CRISPR/Cas13a system with conjugation of Recombinase polymerase amplification (RPA). We evaluated the sensitivity of this combined technology by comparing it with the positive plasmid standard and the genome standard of <em>N. bombycis</em>. Remarkably, the sensitivity of the CRISPR/Cas13a system for <em>N. bombycis</em> positive plasmid standard based on the microplate reader, portable fluorescence detection device, and test strips was 1 copy/μL, 10 copies/μL, and 1 copy/μL, respectively, while for the <em>N. bombycis</em> genome standards, the detection sensitivity was 10 fg/μL, 10 fg/μL, and 1 fg/μL, respectively. In addition, extensive evaluations have demonstrated that the established technology can accurately detect <em>N. bombycis</em> without cross-reactivity with other pathogens, ensuring a specificity rate of 100%. In brief, this study will provide a practical, efficient, and affordable method for early and rapid detection of <em>N. bombycis</em> in various settings.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"175 ","pages":"Article 104203"},"PeriodicalIF":3.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Larval development of a parasitoid depends on host ecdysteroids 寄生虫的幼虫发育取决于寄主的蜕皮激素。

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-16 DOI: 10.1016/j.ibmb.2024.104195

Xinyi Liu , Zhe Jiang , Wei Sun , Jialei Lu , Jiaru He , Yijie Wang , Fanchi Li , Bing Li , Jing Wei

{"title":"Larval development of a parasitoid depends on host ecdysteroids","authors":"Xinyi Liu , Zhe Jiang , Wei Sun , Jialei Lu , Jiaru He , Yijie Wang , Fanchi Li , Bing Li , Jing Wei","doi":"10.1016/j.ibmb.2024.104195","DOIUrl":"10.1016/j.ibmb.2024.104195","url":null,"abstract":"<div><div>Parasitoids often exhibit high flexibility in their development depending on stages of their host at the parasitism, yet little is known about the mechanism underlying such flexibility. In the study, we evaluated the larval development time of the parasitoid <em>Exorista sorbillans</em> (Diptera: Tachinidae) on the lepidopteran model insect <em>Bombyx mori</em> (Lepidoptera: Bombycidae). We found that the development duration of <em>E. sorbillans</em> larvae parasitizing on the late-developmental silkworms was significantly shorter than that of the larvae parasitizing on the early-developmental hosts. Intriguingly, the 2nd-3rd instar molting of parasitoid always occurred when the ecdysteroid titers in the host were increased to higher levels. Furthermore, inhibiting the release of ecdysteroids to parasitic abdomen by thorax-abdomen ligation of the host only repressed the 2nd-instar growth and molting of <em>E. sorbillans</em> larvae, but had no effect on their pupation. Meanwhile, the ecdysone synthesis and 20-hydroxyecdysone (20 E) signaling in larval parasitoids were impeded after ligation treatment. Moreover, exogenous 20 E application could largely rescue the defect in 2nd instar growth and molting through stimulating ecdysone synthesis and signaling in <em>E. sorbillans</em>. Our results indicate that the parasitoid requires the host ecdysteroids to stimulate 20 E signaling and the subsequent 2nd-instar growth and molting. These findings will improve our understanding of the host utilization strategies of parasitoids, and contribute to the development of <em>in vitro</em> rearing procedures of tachinid parasitoids for biological control.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104195"},"PeriodicalIF":3.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent switched taste behavior to ribose 与年龄有关的核糖味觉转换行为

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-13 DOI: 10.1016/j.ibmb.2024.104194

Jiun Sang, Youngseok Lee

引用次数: 0

Hemolymph protease-17b activates proHP6 to stimulate melanization and Toll signaling in Manduca sexta 血淋巴蛋白酶-17b能激活proHP6，从而刺激狐猴的黑化和Toll信号传导

IF 3.2 2区农林科学

Insect Biochemistry and Molecular Biology Pub Date : 2024-10-13 DOI: 10.1016/j.ibmb.2024.104193

Yang Wang, Haobo Jiang

{"title":"Hemolymph protease-17b activates proHP6 to stimulate melanization and Toll signaling in Manduca sexta","authors":"Yang Wang, Haobo Jiang","doi":"10.1016/j.ibmb.2024.104193","DOIUrl":"10.1016/j.ibmb.2024.104193","url":null,"abstract":"<div><div><em>Manduca sexta</em> hemolymph protease-6 (HP6) plays a central role in coordinating antimicrobial responses, such as prophenoloxidase (PPO) activation and Toll signaling. Our previous studies indicated that HP5 and GP6 activate proHP6 in larval hemolymph and extraembryonic tissues, respectively. Here, we report the characterization of HP17b as another HP6 activating enzyme and its regulation by multiple serpins in hemolymph. The precursor of HP17b expressed in baculovirus infected Sf9 cells became spontaneously cleaved at two sites, and these products were purified together in one preparation named HP17b′, a mixture of proHP17b, a 35 kDa intermediate, and HP17b. HP17b′ converted proHP6 to HP6. As reported before, HP6 converted precursors of PPO activating protease-1 (PAP1) and HP8 to their active forms. HP8 activates proSpӓtzle-1 to turn on Toll signaling. We found HP17b′ directly activated proSPHI and II to form a cofactor for PPO activation by PAP1. Supplementation of larval hemolymph with HP17b′, HP17b, or proHP17b significantly increased PPO activation. Adding <em>Micrococcus luteus</em> to the reactions did not enhance PPO activation in the reactions containing HP17b′, HP17b, or proHP17b. Using HP17b antibodies, we isolated from induced plasma HP17b fragments and associated proteins (<em>e.g</em>., serpin-4). Serpin-1A, 1J, 1J′, 4, 5, or 6 reduced the activation of proHP6 by HP17b’ through formation of covalent complexes with active HP17b. We detected an activity for proHP17b cleavage in hemolymph from bar-stage pharate pupae but failed to purify the protease due to its high instability. Other known HPs did not activate proHP17b <em>in vitro</em>. Together, these results suggest that HP17b is a clip-domain protease activated by an unknown endopeptidase in response to a danger signal and regulated by multiple serpins.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"174 ","pages":"Article 104193"},"PeriodicalIF":3.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0