The role of insulin receptor InR in photoperiod-regulated reproductive diapause of Chrysoperla nipponensis

IF 3.2 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology Pub Date : 2025-03-29 DOI:10.1016/j.ibmb.2025.104305

Xiao Wang , Minghui Xu , Xue Kong , Shaofeng Zhong , Jeremiah Joe Kabissa , Dandan Li , Zhiwei Kang , Yongyu Xu , Zhenzhen Chen

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Abstract

Insects usually diapause, a process regulated by hormonal signals as an adaptive mechanism developed through long-term evolution to survive unfavorable environmental conditions. Chrysoperla nipponensis is classified as a photoperiod-sensitive insect. Treatments with short-day (SD) and long-day (LD) conditions have distinct effects on ovarian development and lipid accumulation in adults, with SD condition inducing diapause. Injecting bovine insulin promoted ovarian development and egg formation in diapause females, while injecting insulin receptor induced diapause-like traits in reproductive females. This study investigate the biological function of insulin signaling in the reproductive diapause of females of C. nipponensis. Under SD treatment the mRNA expression level of InR1 and InR2, as well as the protein expression level of InR1 were significantly reduced. This reduction led to stagnant ovarian development, increased adipose tissue mass, and a significant rise in triglyceride (TG) content. Silencing InR1 under LD conditions resulted in halted ovarian development and enhanced lipid accumulation, with the expression levels of Akt, Kr-h1, and Vg significantly decreased mirroring those observed under SD conditions. Interestingly, silencing InR2 under LD condition did not affect ovarian development. Furthermore, transcriptome analysis identified six genes (Akt, PkN, Skp2, CycB3, BTrC, and AurkA) associated with reproductive regulation and eight genes (FadΔ11, EchA, EcI, Ugts (2A3, 1–9), AR, Gpdh and Cbr) linked to lipid metabolism, all of which are involved in InR1 mediated regulation of C. nipponensis reproduction.

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胰岛素受体InR在日本蚕蛹光周期调节生殖滞育中的作用

昆虫通常通过激素信号调控的滞育过程，是一种长期进化形成的适应机制，以适应不利的环境条件。日本金蝶是一种光周期敏感昆虫。短日（SD）和长日（LD）处理对成虫卵巢发育和脂质积累有明显影响，长日处理诱导滞育。注射牛胰岛素促进滞育雌性卵巢发育和卵子形成，而注射胰岛素受体诱导生殖雌性滞育样性状。本研究探讨了胰岛素信号在日本绒猴雌性生殖滞育中的生物学作用。SD处理显著降低了InR1、InR2 mRNA表达量以及InR1蛋白表达量。这种减少导致卵巢发育停滞，脂肪组织质量增加，甘油三酯（TG）含量显著上升。在LD条件下沉默InR1导致卵巢发育停止，脂质积累增加，Akt、Kr-h1和Vg的表达水平显著降低，与SD条件下观察到的结果一致。有趣的是，在LD条件下沉默InR2并不影响卵巢发育。此外，转录组分析还发现了6个与生殖调节相关的基因（Akt、PkN、Skp2、CycB3、BTrC和AurkA）和8个与脂质代谢相关的基因（FadΔ11、EchA、EcI、Ugts （2A3, 1-9）、AR、Gpdh和Cbr），这些基因都参与了InR1介导的日本仙子生殖调节。

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来源期刊

Insect Biochemistry and Molecular Biology 生物-昆虫学

CiteScore

7.40

自引率

5.30%

发文量

105

审稿时长

40 days

期刊介绍： This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.