{"title":"Sec61β是内质网Sec61复合体的一个亚基,与Ocnus协同调节果蝇精子发生","authors":"Qian Wang, Xin Chen, Yu-Feng Wang","doi":"10.1016/j.ibmb.2025.104310","DOIUrl":null,"url":null,"abstract":"<div><div><em>sec61β</em> encodes a subunit of the Sec61 translocon which is a highly conserved heterotrimer responsible for translocating the nascent polypeptides into the lumen of the endoplasmic reticulum (ER) or onto the ER membrane. In this study, we show that knockdown of <em>sec61β</em> in the early germline leads to male sterility in <em>Drosophila melanogaster.</em> These males exhibit testes that are dramatically reduced in size and devoid of germ cells. However, the somatic cells with hub markers extend abnormally beyond the stem cell niche region. Stat92E-positive cells are also expanded into the posterior region of the small testes and primarily in the nuclei. Through tracking the developmental processes of germ cells, we find that the loss of germ cells occurs during the 3rd instar larval stage. Additionally, studies in <em>Drosophila</em> S2 cells reveal that Sec61β can directly interact with Ocnus (Ocn), likely at the nuclear membrane. Genetically, we show that overexpression of <em>ocn</em> partially restores fertility in <em>sec61β</em> knockdown males, while overexpression of <em>sec61β</em> fails to compensate for the defects in male fertility induced by <em>ocn</em> knockdown. These findings suggest that <em>Sec61β</em> might play a critical role in testis development and spermatogenesis, potentially coordinating with Ocn and involving in the JAK/STAT pathway.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"180 ","pages":"Article 104310"},"PeriodicalIF":3.2000,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sec61β, a subunit of the Sec61 complex at the endoplasmic reticulum, coordinates with Ocnus in regulating Drosophila spermatogenesis\",\"authors\":\"Qian Wang, Xin Chen, Yu-Feng Wang\",\"doi\":\"10.1016/j.ibmb.2025.104310\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>sec61β</em> encodes a subunit of the Sec61 translocon which is a highly conserved heterotrimer responsible for translocating the nascent polypeptides into the lumen of the endoplasmic reticulum (ER) or onto the ER membrane. In this study, we show that knockdown of <em>sec61β</em> in the early germline leads to male sterility in <em>Drosophila melanogaster.</em> These males exhibit testes that are dramatically reduced in size and devoid of germ cells. However, the somatic cells with hub markers extend abnormally beyond the stem cell niche region. Stat92E-positive cells are also expanded into the posterior region of the small testes and primarily in the nuclei. Through tracking the developmental processes of germ cells, we find that the loss of germ cells occurs during the 3rd instar larval stage. Additionally, studies in <em>Drosophila</em> S2 cells reveal that Sec61β can directly interact with Ocnus (Ocn), likely at the nuclear membrane. Genetically, we show that overexpression of <em>ocn</em> partially restores fertility in <em>sec61β</em> knockdown males, while overexpression of <em>sec61β</em> fails to compensate for the defects in male fertility induced by <em>ocn</em> knockdown. These findings suggest that <em>Sec61β</em> might play a critical role in testis development and spermatogenesis, potentially coordinating with Ocn and involving in the JAK/STAT pathway.</div></div>\",\"PeriodicalId\":330,\"journal\":{\"name\":\"Insect Biochemistry and Molecular Biology\",\"volume\":\"180 \",\"pages\":\"Article 104310\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-04-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Insect Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0965174825000542\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174825000542","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Sec61β, a subunit of the Sec61 complex at the endoplasmic reticulum, coordinates with Ocnus in regulating Drosophila spermatogenesis
sec61β encodes a subunit of the Sec61 translocon which is a highly conserved heterotrimer responsible for translocating the nascent polypeptides into the lumen of the endoplasmic reticulum (ER) or onto the ER membrane. In this study, we show that knockdown of sec61β in the early germline leads to male sterility in Drosophila melanogaster. These males exhibit testes that are dramatically reduced in size and devoid of germ cells. However, the somatic cells with hub markers extend abnormally beyond the stem cell niche region. Stat92E-positive cells are also expanded into the posterior region of the small testes and primarily in the nuclei. Through tracking the developmental processes of germ cells, we find that the loss of germ cells occurs during the 3rd instar larval stage. Additionally, studies in Drosophila S2 cells reveal that Sec61β can directly interact with Ocnus (Ocn), likely at the nuclear membrane. Genetically, we show that overexpression of ocn partially restores fertility in sec61β knockdown males, while overexpression of sec61β fails to compensate for the defects in male fertility induced by ocn knockdown. These findings suggest that Sec61β might play a critical role in testis development and spermatogenesis, potentially coordinating with Ocn and involving in the JAK/STAT pathway.
期刊介绍:
This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.