Sec61β是内质网Sec61复合体的一个亚基,与Ocnus协同调节果蝇精子发生

IF 3.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qian Wang, Xin Chen, Yu-Feng Wang
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引用次数: 0

摘要

Sec61 β编码Sec61易位子的一个亚基,Sec61易位子是一个高度保守的异源三聚体,负责将新生多肽易位到内质网管腔或内质网膜上。在这项研究中,我们发现在果蝇早期种系中敲低sec61β会导致雄性不育。这些雄性的睾丸尺寸大大缩小,而且没有生殖细胞。然而,具有枢纽标记的体细胞异常地延伸到干细胞生态位区域之外。stat92e阳性细胞也可扩展到睾丸后部,主要分布在细胞核内。通过对生殖细胞发育过程的跟踪,我们发现生殖细胞的丢失发生在3龄幼虫阶段。此外,在果蝇S2细胞中的研究表明,Sec61β可以直接与Ocnus (Ocn)相互作用,可能在核膜上。从遗传学上讲,我们发现在sec61β敲低的雄性中,过表达ocn可以部分恢复生育能力,而过表达sec61β不能补偿ocn敲低导致的雄性生育缺陷。这些发现表明Sec61β可能在睾丸发育和精子发生中发挥关键作用,可能与Ocn协调并参与JAK/STAT通路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sec61β, a subunit of the Sec61 complex at the endoplasmic reticulum, coordinates with Ocnus in regulating Drosophila spermatogenesis

Sec61β, a subunit of the Sec61 complex at the endoplasmic reticulum, coordinates with Ocnus in regulating Drosophila spermatogenesis
sec61β encodes a subunit of the Sec61 translocon which is a highly conserved heterotrimer responsible for translocating the nascent polypeptides into the lumen of the endoplasmic reticulum (ER) or onto the ER membrane. In this study, we show that knockdown of sec61β in the early germline leads to male sterility in Drosophila melanogaster. These males exhibit testes that are dramatically reduced in size and devoid of germ cells. However, the somatic cells with hub markers extend abnormally beyond the stem cell niche region. Stat92E-positive cells are also expanded into the posterior region of the small testes and primarily in the nuclei. Through tracking the developmental processes of germ cells, we find that the loss of germ cells occurs during the 3rd instar larval stage. Additionally, studies in Drosophila S2 cells reveal that Sec61β can directly interact with Ocnus (Ocn), likely at the nuclear membrane. Genetically, we show that overexpression of ocn partially restores fertility in sec61β knockdown males, while overexpression of sec61β fails to compensate for the defects in male fertility induced by ocn knockdown. These findings suggest that Sec61β might play a critical role in testis development and spermatogenesis, potentially coordinating with Ocn and involving in the JAK/STAT pathway.
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来源期刊
CiteScore
7.40
自引率
5.30%
发文量
105
审稿时长
40 days
期刊介绍: This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.
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