Kartika Jurnal Ilmiah Farmasi最新文献

{"title":"Profil fitokimia selada laut (Ulva lactuca) dan mikro alga filamen (Spirogyra sp) sebagai bahan alam bahari potensial dari perairan Indonesia","authors":"Ari Sri Windyaswari, Elfahmi Elfahmi, Fahrauk Faramayuda, Soraya Riyanti, Oktiyas Muzaky Luthfi, Inna Puspa Ayu, N. T. Pratiwi, Khairunisa Harizqi Nurul Husna, Ridzka Magfirah","doi":"10.26874/kjif.v7i2.288","DOIUrl":"https://doi.org/10.26874/kjif.v7i2.288","url":null,"abstract":"<p align=\"center\"><strong>Abstrak</strong></p><p>Perairan Indonesia merupakan habitat bagi berbagai spesies makro dan mikro alga. Selada laut <em>(Ulva lactuca)</em> adalah salah satu makroalga hijau yang secara empiris digunakan sebagai makanan oleh masyarakat Indonesia yang hidup dipesisir pantai. Pada perairan tawar, ganggang hijau (<em>Spirogyra porticalis</em>) merupakan mikroalga filamen yang berperan penting sebagai bioindikator dalam sistem akuatik. Penelitian ini bertujuan untuk mengetahui profil fitokimia dari selada laut dan ganggang hijau yang tumbuh di perairan Indonesia. Hasil pengujian kualitatif pada selada laut <em>(Ulva lactuca)</em>  menunjukkan kandungan metabolit primer  dan sekunder berturut-turut adalah karbohidrat, alkaloid, flavonoid, mono dan seskuiterpenoid. Sementara itu ganggang hijau (<em>Spirogyra porticalis</em>) mengandung karbohidrat, protein, alkaloid, flavonoid, fenolik, tannin, kuinon, mono dan seskuiterpenoid. Pola kromatogram selada laut <em>(Ulva lactuca) </em>dan  ganggang hijau (<em>Spirogyra porticalis)</em> mendeteksi aktivitas antioksidan yang dimiliki oleh senyawa- metabolit sekunder  seperti fenol, tannin, flavonoid, mono dan seskuiterpenoid yang memiliki variasi kepolaran dari semi hingga polar. Hasil uji pendahuluan aktivitas antioksidan menggunakan metode dinamolisis menunjukkan selada laut <em>(Ulva lactuca) </em>dan  ganggang hijau (<em>Spirogyra porticalis)</em> memiliki aktivitas antioksidan dan berpotensi dikembangkan sebagai obat, suplemen, eksipien farmasi dan makanan nutrisi.</p><p align=\"center\"> </p><p>Kata kunci: selada laut <em>(Ulva lactuca),</em> ganggang hijau (<em>Spyrogyra porticalis</em>), antioksidan, bahari, Indonesia.</p><p> </p><p align=\"center\"><strong>Abstract</strong></p><p><em>Indonesian waters are habitat for various macro and micro algae species. Sea lettuce </em><span style=\"text-decoration: underline;\">Ulva</span> <span style=\"text-decoration: underline;\">lactuca</span><em> is one of the green macroalgae that is empirically used as food by Indonesian people. In freshwater, green algae </em><span style=\"text-decoration: underline;\">Spirogyra</span> <span style=\"text-decoration: underline;\">porticalis</span><em> is filament microalgae that play an important role as bioindicators in the aquatic system. The aim of this research was to profiling the chemical constituent from native Indonesian sources,</em><span style=\"text-decoration: underline;\"> Ulva</span> <span style=\"text-decoration: underline;\">lactuca</span><em>  and</em> <span style=\"text-decoration: underline;\">Spirogyra</span> <span style=\"text-decoration: underline;\">porticalis</span><em>. The results of  </em><span style=\"text-decoration: underline;\">Ulva</span> <span style=\"text-decoration: underline;\">lactuca</span><em>  qualitative analysis showed the content of primary and secondary metabolites are carbohydrates, alkaloids, flavonoids, mono, and sesquiterpenoids, respectively. On the other hand, green algae","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"117 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88508541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hubungan sikap dan hambatan terhadap persepsi mahasiswa farmasi tentang complementary and alternative medicine (CAM)","authors":"R. Rubiyanti","doi":"10.20885/JIF.VOL15.ISS1.ART4","DOIUrl":"https://doi.org/10.20885/JIF.VOL15.ISS1.ART4","url":null,"abstract":"Intisari Latar Belakang : Memasuki era globalisasi, pola penyakit di negara berkembang telah berubah dari penyakit infeksi menjadi penyakit tidak menular seperti penyakit kardiovaskular, diabetes, hipertensi, depresi, dan lainnya. Gaya hidup, diet, kegemukan, kurang olah raga, dan stres merupakan faktor penyebab penyakit tidak menular ini. Pendekatan dengan menggunakan CAM dan TM ini secara khusus penting dalam pengembangan strategi perawatan kesehatan untuk negara berkembang yang telah banyak digunakan di negara maju. Tujuan : Pendidikan kesehatan yang sedang dilakukan oleh mahasiswa farmasi menjadi hal yang sangat penting dalam mensosialisasikan mengenai pengobatan CAM atau TM Metode: Metode penelitian yang digunakan adalah kuantitatif desain potong lintang dengan 67 responden. Analisis dilakukan secara kuantitatif menggunakan U man-Whitney. Hasil : Hasil penelitian menunjukkan hasil positif terhadap rekomendasi CAM, namun masih kurang untuk penggunaan CAM sendiri. Kesimpulan: Metode CAM yang masuk dalam materi perkuliahan memiliki nilai yang lebih tinggi dibandingkan dengan metode lain yang tidak masuk dalam materi baik untuk angkatan 2016 dan angkatan 2017. Kata Kunci : CAM, TM, sikap, pengetahuan, metode pengobatan, kesehatan The Relationship between behavior and barrier on pharmacy students perceptions of Complementary and Alternative Medicine (CAM) Abstract Background : In globalization, disease in developing countries have changed from infectious diseases to degenerative diseases such as cardiovascular disease, diabetes, hypertension, depression, and others. Lifestyle, diet, obesity, lack of exercise, and stress are factors that cause degenerative diseases. CAM and TM is especially important in the development of health care strategies for developing countries that have been widely used in developed countries. Objective : Institutions is being carried out by students is very important in socializing CAM or TM, including pharmacy students. Method : The research method used was a quantitative cross-sectional design with 67 respondents. The analysis was done quantitatively using U man-Whitney. Results: The result indicate a possitive attitude toward CAM recommendations, but still less for the use of CAM. compared to other methods not included in the material for both the students group of 2016 and 2017 Conclusion : The CAM method included in the lecture material has a higher value. Keywords: CAM, TM, attitudes, knowledge, treatment methods, health","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74712665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluasi kerasionalan dan kuantitas penggunaan antihipertensi pada pasien gagal jantung di instalasi rawat inap rumah sakit PKU Muhammadiyah Gamping Yogyakarta","authors":"Lola Lolita, Asih Istiani","doi":"10.20885/JIF.VOL15.ISS1.ART5","DOIUrl":"https://doi.org/10.20885/JIF.VOL15.ISS1.ART5","url":null,"abstract":"Intisari Latar belakang:  Terapi anti hipertensi pada pasien gagal jantung digunakan untuk menguraagi progresifitas, mengurangi resiko infark miokard serta kematian mendadak akibat gagal jantung. Penggunaan obat dikatakan rasional jika pasien diberikan obat sesuai dari segi indikasi, pemilihan, dosis, aturan dan lama penggunaan, yang memenuhi kebutuhan individu pasien. Evaluasi penggunaan obat bertujuan mengidentifikasi dan meminimalisasi masalah terkait obat serta menjamin pengobatan yang sesuai dengan kebutuhan pasien dalam periode waktu yang adekuat dengan harga yang terjangkau. Tujuan:  Mengevaluasi rasionalitas dan kuantitas penggunaan antihipertensi pada pasien gagal jantung rawat inap di RS PKU Muhammadiyah Gamping Yogyakarta Metode:  Observational deskriptif dengan pengambilan data melalui rekam medis secara retrospektif pada Januari-Desember tahun 2016. Rasionalitas diperoleh dengan menghitung persentase jumlah kasus rasional dibagi dengan jumlah total kasus. Sedangkan perhitungan kuantitas penggunaan obat antihipertensi dengan menggunakan metode ATC/DDD. Hasil:  Terdapat 106 kasus yang terinklusi dengan 50% pasien berjenis kelamin pria dan 50% pasien berjenis kelamin perempuan. Persentase tertinggi rentang usia responden berkisar 65-74 tahun. Rata-rata total jumlah hari rawat pasien di RS PKU Muhammadiyah Gamping Yogyakarta tahun 2016 adalah 6 hari. Kesimpulan:  Penelitian menunjukkan bahwa ditemukan 100% tepat obat, 94% tepat pasien, 38% tepat dosis serta 36% dikatakan rasional. Jenis antihipertensi yang banyak digunakan di Instalasi Rawat Inap RS PKU Muhammadiyah Gamping Yogyakarta tahun 2016 yaitu furosemid 76,6 DDD/100  patient-days . Hal ini berarti 76,6% dari pasien gagal jantung rawat inap memperoleh 1 DDD obat furosemide setiap hari Kata kunci :  Antihipertensi, gagal jantung, rawat inap, rasionalitas, ATC/DDD Evaluation of rationality and q uantity of a nti- h ypertension u se in h eart f ailure p atients in inpatient department of PKU Muhammadiyah Gamping Yogyakarta Hospital Abstract Background: Anti hypertensive therapy in patients with heart failure aims to reduce disease proggession, risk of myocardial infaction and sudden death from heart failure. Rational drug use defines that “patient receive appropriate medication in terms of adequate indications, drug selection, route of administration, therapy duration that met their own individual requirements. Drug use evaluation aims to identify drug related problems and ensure the best therapy in accordance with patient needs within an adequate timeframe at an affordable price. Objective: To assess quantity and rationality of antihypertensive drug use in patients with heart failure at PKU Muhammadiyah Hospital, Gamping Yogyakarta Method: Observational descriptive with retrospective data retrieval from January to December 2016. Rationality was obtained by calculating the percentage of rational cases divided by total number of cases. While calculating the antihypertensive utilizat","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82029675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studi potensi ubi kelapa (Dioscorea alata. L) sebagai bahan penghancur tablet","authors":"Haeria Doloking, Nurismi Dhuha, Pratiwi Ningsi","doi":"10.20885/JIF.VOL15.ISS1.ART1","DOIUrl":"https://doi.org/10.20885/JIF.VOL15.ISS1.ART1","url":null,"abstract":"Intisari Latar Belakang: Salah satu upaya untuk mewujudkan kemandirian produksi bahan baku farmasi Indonesia adalah dengan memanfaatkan pabrik sebagai sumber. Dioscorea alata L sebagai salah satu sumber pati perlu dipelajari untuk pengembangan potensial sebagai tablet disintegran. Tujuan: Tujuan penelitian ini adalah untuk mengetahui karakteristik pati Dioscorea alata L. dan mengetahui potensi sebagai bahan disintegran tablet. Metode: Analisis karakteristik meliputi proksimat, kandungan amilosa, morfologi granular, kristalinitas, kekuatan pengembangan, dan pengikatan kapasitas air. Studi potensial sebagai disintegran dilakukan dengan merumuskan tablet piroksikam menggunakan pati Dioscorea alata dibandingkan dengan pati jagung. Evaluasi sifat-sifat disintegran dilakukan dengan uji disintegrasi, uji kerapuhan, uji kekerasan, dan uji disolusi tablet. Hasil: Berdasarkan studi karakteristik pati Dioscorea alata L., kadar air, abu, protein, dan lemak masing-masing adalah 13,08%, 0,23%, 1,43%, dan 0,81%. Kemudian, Amilosa adalah 18,08%. Daya bengkak dan pengikatan kapasitas air menunjukkan 1,21 dan 3,31. Analisis morfologi granular menunjukkan bentuk ellipsoid dan bola. Kristal pati menunjukkan bentuk semikristal dengan pola kristal ortorombik. Uji disintegrasi tablet menunjukkan bahwa formula I dan II, memiliki waktu hancur 3,50 dan 4,25. Uji kelayakan formula I dan formula II adalah 0,011% dan 0,008%. Uji kekerasan Formula I dan Formula II menunjukkan 5kg dan 6kg. Uji disolusi Formula I dan Formula II menunjukkan 88,85% dan 85,58%. Kesimpulan: Dari hasil tersebut, pati Dioscorea alata L. berpotensi sebagai bahan penghancur tablet Kata kunci: Dioscorea alata L., tablet, disintegrant, pati, bahan baku Potential Study of Ubi Kelapa (Dioscorea Alata. L) Starch as Tablet Desintegrant Material Abstract Background: One of the efforts to realize the sovereignty of Indonesia's pharmaceutical raw material production is to utilize the plant as a source. Dioscorea alata L as one of the starch sources needs to be studied for potential development as a tablet disintegrant. Objective: The aims of the research are to determine  the characteristic of Dioscorea alata L. starch and to find out the potential as tablet disintegrant material. Method: Characteristic analysis include are proximate, amylose content, granular morphology, crystallinity, swelling power, and water capacity binding. The potential study as disintegrant  was performed by formulating a piroxicam tablet using  Dioscorea alata starch compared to corn starch. Evaluation of the disintegrant properties was performed by disintegration test, friability test, hardness test, and dissolution test of the tablets. Results: Based on the characteristic study of Dioscorea alata L. starch, water, ash, protein, and fat contents are 13.08%, 0.23%, 1.43%, and 0.81%, respectively. Then, Amylose is 18.08%. Swelling power and water capacity binding  shows 1.21 and 3.31. Glanular morphology analysis shows ellipsoid and sph","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79829668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluasi pelayanan kefarmasian pada pasien rawat jalan di rumah sakit PKU Muhammadiyah Yogyakarta","authors":"Achmad Saiful, Diesty Anita Nugraheni, Dian Medisa","doi":"10.20885/JIF.VOL15.ISS1.ART3","DOIUrl":"https://doi.org/10.20885/JIF.VOL15.ISS1.ART3","url":null,"abstract":"Abstract Background: WHO found that the inappropriate use of medicine still become a big problem in the world. Therefore, pharmacy services evaluation must be done to improve the appropriate use of medicine. Objective: This study aims to know the pharmacy services based on WHO patient-care indicators and to determine the correlations between socio-demographic characteristics and patient knowledge about medicine use. Method: An observational cross-sectional study was conducted by using the WHO patient-care indicator on 211 regular outpatients or non-insurance at one of private hospital in Yogyakarta. This study used disproportionate stratified random sampling method. Data were collected by observation and interview the patient and analyzed by using WHO patient-care indicator. The relation between socio-demographic characteristics and patient knowledge were analyzed using chi-square and spearmen test. Results: The average of dispensing time was 47.52 second and 99.4% medicines dispensed. Percentage of medicine labelled was 92.26% and only 36,5% patients know about the medicines use. Based on statistical analysis, there was no correlation between level of patient knowledge with age ( p=0.218 ) and gender ( p=0.209 ). Otherwise, education ( p=0.005 ) was correlated with level of patient knowledge. Conclusion: The pharmacy services in hospital was good, but pharmacist still need to improve communication to patients about medicines they received . Whereas, education have relationship with patient level knowledge Keywords: pharmacy service, outpatient, hospital Latar belakang: Data WHO menyatakan bahwa masih banyak terjadi penggunaan obat yang tidak tepat oleh pasien. Oleh karena itu, perlu dilakukan dengan evaluasi pelayanan kefarmasian secara rutin sebagai salah satu upaya peningkatan penggunaan obat yang tepat. Tujuan: Mengetahui gambaran pelayanan kefarmasian pada pasien rawat jalan umum berdasarkan indikator pelayanan pasien WHO dan mengetahui hubungan faktor sosiodemografi dengan pengetahuan pasien tentang penggunaan obat Metode: Penelitian ini merupakan penelitian observasional dengan menggunakan rancangan cross- sectional pada 211 pasien rawat jalan umum atau non-asuransi di salah satu rumah sakit swasta Yogyakarta. Pengambilan sampel dilakukan dengan metode disproportionate stratified random sampling . Data diperoleh dari observasi dan wawancara kepada pasien kemudian data dianalisis secara deskriptif menggunakan rumus sesuai indikator pelayanan pasien WHO. Analisis hubungan sosiodemografi dengan pengetahuan pasien tentang penggunaan obat dilakukan menggunakan uji statistik chi-square dan spearman test . Hasil: Rata-rata waktu penyerahan obat yaitu 47,52 detik dengan persentase obat terlayani 99,4%. Persentase etiket obat yang memadai 91,7% dan pasien yang mengetahui cara penggunaan obat yang diterima sebesar 36,5%. Hasil analisis statistik menunjukkan tidak ada hubungan antara usia ( p=0,218 ) dan jenis kelamin ( p=0,209 ) dengan tingkat penge","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74529683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulasi Sediaan Mikroemulsi Gel Anti Jerawat Mengandung Kombinasi Minyak Jinten Hitam (Nigella sativa L.) dan Minyak Zaitun (Olea europaea L.)","authors":"Sani Ega Priani, W. Dewi, A. Gadri","doi":"10.26874/KJIF.V6I2.143","DOIUrl":"https://doi.org/10.26874/KJIF.V6I2.143","url":null,"abstract":"<p align=\"center\"><strong>Abstrak</strong></p><p align=\"center\"><strong> </strong></p><p class=\"Default\">Jerawat adalah penyakit kulit yang paling sering terjadi dan timbul akibat penyumbatan atau inflamasi pada kelenjar pilosebasea. <em>Propionibacterium acnes </em>diketahui merupakan bakteri utama pemicu terjadinya inflamasi pada jerawat. Minyak jinten hitam dan minyak zaitun diketahui memiliki aktivitas antibakteri sehingga dapat dikembangkan untuk pengobatan jerawat karena infeksi. Penelitian ini bertujuan untuk menguji aktivitas antibakteri kombinasi minyak jinten hitam dan minyak zaitun terhadap <em>P. acnes</em> dan memformulasikannya ke dalam bentuk sediaan mikroemulsi gel. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar pada konsentrasi kombinasi minyak 0,25; 0,5; dan 1%. Formulasi sediaan mikroemulsi gel dilakukan dengan menggunakan <em>cremophor RH </em>40 sebagai surfaktan, gliserin sebagai kosurfaktan, dan viscolam mac 10 sebagai <em>gelling agent</em>. Karakterisasi sediaan meliputi pengujian organoleptis, pH, viskositas, sifat alir, ukuran globul, dan stabilitas termodinamik. Hasil penelitian menunjukkan bahwa konsentrasi minyak 0,5% sudah memberikan aktivitas antibakteri terhadap <em>P. acnes</em> dengan diameter hambat 12,47±1,07mm. Formula optimum mikroemulsi gel adalah formula yang mengandung minyak (6%), <em>cremophor RH </em>40 (35%), gliserin (35%), dan gel viskolam 20%. Sediaan mikroemulsi gel memiliki rata rata ukuran globul 120 nm yang memenuhi persyaratan ukuran globul mikroemulsi. Sediaan mikroemulsi gel stabil berdasarkan hasil uji stabilitas termodinamik dengan tidak terlihat adanya pemisahan fase.</p><p><strong> </strong></p><p><strong>Kata kunci</strong> :   Minyak jinten hitam, Minyak zaitun, <em>P</em><em>.</em><em> acnes</em><em>, </em>Mikroemulsi gel</p><p> </p><p align=\"center\"><strong><em>Formulation of anti acne microemulsion gel containing combination of black seed oil </em></strong><strong><em>(Nigella sativa L.) </em></strong><strong><em> and olive oil </em></strong><strong><em>(Olea europaea L.)</em></strong><strong><em> </em></strong></p><p align=\"center\"><strong><em> </em></strong></p><p align=\"center\"><strong><em>Abstract</em></strong><strong><em></em></strong></p><p align=\"center\"><strong><em> </em></strong></p><p><em>Acne vulgaris is the most common </em><em>skin disease involving blockage and/or inflammation of pilosebaceous unit. </em><em>Propionibacterium acnes</em><em>  has</em><em> been recognized as pus-forming bacteria triggering an inflammation in acne</em><em>. Black seed and olive oil are herbal medicines that known have antibacterial activity, that could be develop for acne treatment. </em><em>This research was conducted to determine antibacterial activity of black seed and olive oil </em><em>combination</em><em> against P. acnes and </em><em>develop the microemulsion gel preparation containing of the oil. </em><em>Antibacterial activity test was conducted by agar diffu","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83883120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANALISIS FRAGMEN DNA DARI BAKTERI PSEUDOMONAS FLUORESCENS SEBELUM DAN SESUDAH IMOBILISASI DALAM κ-KARAGENAN","authors":"Syarif Hamdani, Isma Oktadiana, Dewi Astriany","doi":"10.26874/KJIF.V6I2.153","DOIUrl":"https://doi.org/10.26874/KJIF.V6I2.153","url":null,"abstract":"<p align=\"center\"><strong>Abstrak</strong></p><p align=\"center\"><strong> </strong></p><p>Imobilisasi merupakan pengurungan fisik atau lokalisasi bakteri utuh dalam lingkungan tertentu untuk memaksimalkan aktivitas biokatalis yang diinginkan. Imobilisasi mampu menyediakan bakteri sebagai biokatalis dalam konsentrasi tinggi sehingga dapat  meningkatkan efisiensi serta produktivitas. Penelitian ini dilakukan untuk mengamati efek imobilisasi terhadap fragmen DNA bakteri setelah penyimpanan dingin selama lima bulan, bakteri yang digunakan adalah <em>Pseudomonas fluorescens</em> yang diimobilisasikan pada κ-karagenan lokal yang berasal dari perairan Karimun Jawa. Pengamatan dilakukan terhadap fragmen DNA setelah direstriksi oleh enzim EcoRI dan dianalisis menggunakan elektroforesis gel. Bakteri diimobilisasi dalam karagenan pada konsentrasi 1% dan 2% (b/v). Hasil menunjukkan bahwa DNA <em>P.</em><em>fluorescens</em> memberikan fragmen identik antara bakteri terimobilisasi dan tanpa imobilisasi.</p><p> </p><p><strong>Kata Kunci</strong> :  Imobilisasi, <em>Pseudomonas fluorescens, </em>Karagenan, Elektroforesis Gel.</p><p align=\"center\"><strong> </strong></p><h2><em>Analysis of Pseudomonas fluorescens’s  </em><em>DNA fragments before and after immobilize in </em><em>κ-carrageenan</em><em> </em></h2><p align=\"center\"><strong><em> </em></strong></p><p align=\"center\"><strong><em>Abstract</em></strong></p><p align=\"center\"><strong><em> </em></strong></p><pre><em>Immobilization is physical confinement or localization of bacteria in a particular environment to maximize biocatalyst activity. Immobilization provide bacteria as biocatalysts in high concentrations in order to increase efficiency and productivity. This study was conducted to observe the effect of immobilization to bacterial DNA fragments after cool storage for five months, bacteria used was Pseudomonas fluorescens that im</em><em>m</em><em>obilized in local κ-carrageenan from Karimun Jawa. Observations conducted to DNA fragments after being restricted by EcoRI enzymes and analyzed using gel electrophoresis. The bacteria were immobilized in carrageenan on concentrations of 1% and 2% (w / v), respectively. Result shown that P.fluorescens's DNA gave similar fragments between im</em><em>m</em><em>obilized and without i</em><em>m</em><em>mobilized.</em><em></em></pre><pre><em> </em></pre><pre><strong><em>Keywords:  </em></strong><em>Immobilization, Pseudomonas fluorescens, Carrageenan, Gel Electrophoresis.</em></pre>","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81360968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potensi Ekstrak Etanol Kulit Jeruk Lemon (Citrus limon L.) Sebagai Obat Alternatif Hiperkolesterolemia Pada Tikus Wistar Hiperglikemik","authors":"D. Hartanto, Ellen Lydia Kurniasari, Ribka Artha Maria, Puspa Sari Dewi, Vina Septiani","doi":"10.26874/KJIF.V6I2.156","DOIUrl":"https://doi.org/10.26874/KJIF.V6I2.156","url":null,"abstract":"<p align=\"center\"><strong>Abstrak</strong></p><p align=\"center\"><strong> </strong></p><p>Jeruk lemon (<em>Citrus limon </em>L) secara empiris telah digunakan masyarakat untuk menurunkan berat badan.  Selain itu perasan jeruk lemon pun dipercaya mampu menurunkan kadar kolesterol darah. Penelitian ini bertujuan untuk menentukan potensi ekstrak etanol kulit jeruk lemon sebagai obat alternatif hiperkolesterolemia pada tikus <em>wistar</em> hiperglikemia dengan menggunakan metode proteksi. Proses ekstraksi kulit buah jeruk lemon dilakukan dengan metode maserasi.  Semua tikus diberi sediaan uji sesuai dengan kelompoknya (kontrol, ekstrak etanol jeruk lemon, pembanding simvastatin) bersamaan dengan intervensi fruktosa 60% yang dicampur dalam makanannya selama 6 minggu. Pemeriksaan kadar kolesterol total dilakukan setiap minggu. Hasil pengujian menunjukkan bahwa ekstrak etanol kulit buah lemon mampu menghambat peningkatan kadar kolesterol total tikus <em>wistar </em>hiperglikemia.  Ekstrak etanol kulit buah lemon dosis 70 mg/kg bb menunjukkan hasil terbaik yang berbeda bermakna dibanding kelompok kontrol (p<0,05) pada penelitian ini. Dengan demikian, ekstrak etanol kulit jeruk lemon berpotensi untuk digunakan sebagai obat alternatif hiperkolesterolemia.</p><p> </p><p><strong>Kata Kunci :</strong> Hiperkolesterolemia, Lemon, Fruktosa 60%</p><p> </p><p align=\"center\"><strong><em>Potency of lemon peel ethanolic extract (Citrus limon L.) as alternative drug for hypercholesterolemia in hyperglycemic wistar rat</em></strong></p><p><em> </em></p><p align=\"center\"><strong><em>Abstract </em></strong></p><p align=\"center\"><strong><em> </em></strong></p><p><em>Lemon (Citrus limon L) empirically has been used by the community to lose weight. Besides lemon juice is also believed to be able to reduce blood cholesterol levels. This study aims to determine the potential of ethanol extract of lemon peel as an alternative medicine for hypercholesterolemia in hyperglycemic wistar rats using the protection method. The process of extracting lemon peel is done by maceration method. All rats were given a test preparation according to their group (control, lemon ethanol extract, compared to simvastatin) together with intervention of fructose 60% mixed in the food for 6 weeks. Examination of total cholesterol levels is carried out every week. The test results showed that the ethanol extract of lemon peel was able to inhibit the increase of total cholesterol levels in hyperglycemic wistar rats. The ethanol extract of lemon peel doses of 70 mg / kg bw showed the best results which were significantly different than the control group (p <0.05) in this study. Thus, the ethanol extract of lemon peel has the potential to be used as an alternative drug for hypercholesterolemia.</em></p><p><em> </em></p><p><strong><em>Keywords</em></strong><em> : Hypercholesterolemia, Lemon, Fructose 60 % </em></p>","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"404 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76349326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Gel Ekstrak Daun Kerehau (Callicarpa longifolia Lam.) Terhadap Penyembuhan Luka Pada Model Tikus Diabetes","authors":"S. Candra, Elis Susilawati, I. K. Adnyana","doi":"10.26874/KJIF.V6I2.154","DOIUrl":"https://doi.org/10.26874/KJIF.V6I2.154","url":null,"abstract":"Abstrak Luka diabetes adalah infeksi, luka dan destruksi pada penderita diabetes melitus. Daun kerehau secara empiris digunakan oleh suku Dayak Tunjung sebagai obat luka dan bengkak. Penelitian ini bertujuan untuk mengetahui pengaruh gel ekstrak daun kerehau terhadap penyembuhan luka pada model tikus diabetes. Induksi diabetes dilakukan menggunakan aloksan dosis 75 mg/kg BB intravena. Model hewan diabetes dibuat luka dengan kedalaman 0,3 cm menggunakan scalpel steril pada bagian punggung. Hewan uji dibagi menjadi lima kelompok dan mendapatkan sediaan basis gel, Prontosan® gel, sediaan gel ekstrak daun kerehau konsentrasi 2,5%, 5%, dan 10%. Pengolesan gel dilakukan dua kali sehari selama 13 hari. Parameter penyembuhan luka dilakukan dengan mengukur panjang luka dan dinyatakan dengan persentase penyembuhan luka. Hasil pengujian menunjukkan bahwa dengan pemberian gel ekstrak daun kerehau dapat mempercepat penyembuhan luka pada model tikus diabetes dimana sediaan gel ekstrak daun kerehau konsentrasi 2,5% memberikan kesembuhan pada hari ke-13, sediaan gel konsentrasi 5% pada hari ke-12, dan sediaan gel konsentrasi 10% pada hari ke-9. Hasil juga menunjukkan rata-rata persentase penyembuhan luka pada kelompok konsentrasi 10% lebih tinggi dibandingkan konsentrasi 2,5% dan 5%. Dapat disimpulkan bahwa pemberian gel ekstrak daun kerehau dapat menyembuhkan luka pada model tikus diabetes. Kata kunci:    Luka diabetes, daun kerehau, penyembuhan luka Effect of Kerehau Leaves Extract Gel to Wound Healing Process  in Diabet Rats Model Abstract Diabetic wounds are infection, injury and destruction in people with diabetes mellitus. Kauhau leaves are empirically used by the Tunjung Dayak tribe as a medicine for wounds and swelling. The aim of this study was to determine the effect of Kerehau leaf extract gel on wound healing in diabetic mouse models. Induction of diabetes is done using alloxan dose of 75 mg / kg BW intravenously. The diabetic animal model was made with a 0.3 cm depth wound using a sterile scalpel on the back. The test animals were divided into five groups and obtained base gel preparations, Prontosan® gel, preparations of Kerehau leaf extract gel concentrations of 2.5%, 5%, and 10%. Gel application is done twice a day for 13 days. Wound healing parameters were performed by measuring the length of the wound and expressed by the percentage of wound healing. The test results showed that administration of Kerehau leaf extract gel could accelerate wound healing in diabetic mouse models where the preparations of Kerehau leaf extract gel with a concentration of 2.5% gave healing on the 13th day, preparations for 5% gel on 12th day, and preparations gel concentration of 10% on day 9. The results also showed that the average percentage of wound healing in the concentration group was 10% higher than the concentration of 2.5% and 5%. It can be concluded that administration of Kerehau leaf extract gel can heal wounds in diabetic mouse models. Keywords:  Diabet","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85466842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FICI VALUE OF AQUILARIA MALACCENSIS LEAVES EXTRACT AND AMOXICILLIN AGAINST PROTEUS MIRABILIS AND PSEUDOMONAS AERUGINOSA","authors":"N. Valentine, P. Apridamayanti, R. Sari","doi":"10.26874/KJIF.V6I2.157","DOIUrl":"https://doi.org/10.26874/KJIF.V6I2.157","url":null,"abstract":"Abstract Infection is a common disease caused by microbes. The use of antimicrobial drugs such as Amoxicillin is most widely used in Indonesia, but has develop resistance. The resistance of Amoxicillin can be overcome by combining it with Karas leaves ( Aquilaria malaccensis Lam.) which has an antibacterial activity. This research was conducted to know the value of Fractional Inhibitory Concentration Index (FICI) from the combination of ethanolic Karas leaves extract ( A. malaccensis Lam.) with amoxicillin in Gram-negative bacteria test, that is, Proteus mirabilis and Pseudomonas aeruginosa. This research was used disc diffusion method by Kirby Bauer. Minimum Inhibitory Concentration (MIC) result of ethanolic karas leaves extract ( A. malaccensis Lam.)  in P. mirabilis and P. aeruginosa was 0.5mg/ml. MIC’s result of amoxicillin in P. mirabilis and P. aeruginosa sequentially were 0.0039 and 0.0625 mg/ml. Then, evaluation of MIC value from combination of ethanolic karas leaves extract and amoxicillin ( 1 / 4 x MIC, 1 / 2 x MIC, 1 x MIC, 2 x MIC and 4 x MIC) showed that the combination of ethanolic karas leaves extract with amoxicillin has characteristics of FICI value on each bacteria, P. aeruginosa : 0.5 (synergistic) and P. mirabilis : 8 (antagonist) Keyword : antibacteria, Aquilaria malaccensis , amoxicillin, FICI","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91053883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}