{"title":"假单胞菌细菌的DNA片段分析FLUORESCENS前后固定在κ-KARAGENAN","authors":"Syarif Hamdani, Isma Oktadiana, Dewi Astriany","doi":"10.26874/KJIF.V6I2.153","DOIUrl":null,"url":null,"abstract":"<p align=\"center\"><strong>Abstrak</strong></p><p align=\"center\"><strong> </strong></p><p>Imobilisasi merupakan pengurungan fisik atau lokalisasi bakteri utuh dalam lingkungan tertentu untuk memaksimalkan aktivitas biokatalis yang diinginkan. Imobilisasi mampu menyediakan bakteri sebagai biokatalis dalam konsentrasi tinggi sehingga dapat meningkatkan efisiensi serta produktivitas. Penelitian ini dilakukan untuk mengamati efek imobilisasi terhadap fragmen DNA bakteri setelah penyimpanan dingin selama lima bulan, bakteri yang digunakan adalah <em>Pseudomonas fluorescens</em> yang diimobilisasikan pada κ-karagenan lokal yang berasal dari perairan Karimun Jawa. Pengamatan dilakukan terhadap fragmen DNA setelah direstriksi oleh enzim EcoRI dan dianalisis menggunakan elektroforesis gel. Bakteri diimobilisasi dalam karagenan pada konsentrasi 1% dan 2% (b/v). Hasil menunjukkan bahwa DNA <em>P.</em><em>fluorescens</em> memberikan fragmen identik antara bakteri terimobilisasi dan tanpa imobilisasi.</p><p> </p><p><strong>Kata Kunci</strong> : Imobilisasi, <em>Pseudomonas fluorescens, </em>Karagenan, Elektroforesis Gel.</p><p align=\"center\"><strong> </strong></p><h2><em>Analysis of Pseudomonas fluorescens’s </em><em>DNA fragments before and after immobilize in </em><em>κ-carrageenan</em><em> </em></h2><p align=\"center\"><strong><em> </em></strong></p><p align=\"center\"><strong><em>Abstract</em></strong></p><p align=\"center\"><strong><em> </em></strong></p><pre><em>Immobilization is physical confinement or localization of bacteria in a particular environment to maximize biocatalyst activity. Immobilization provide bacteria as biocatalysts in high concentrations in order to increase efficiency and productivity. This study was conducted to observe the effect of immobilization to bacterial DNA fragments after cool storage for five months, bacteria used was Pseudomonas fluorescens that im</em><em>m</em><em>obilized in local κ-carrageenan from Karimun Jawa. Observations conducted to DNA fragments after being restricted by EcoRI enzymes and analyzed using gel electrophoresis. The bacteria were immobilized in carrageenan on concentrations of 1% and 2% (w / v), respectively. Result shown that P.fluorescens's DNA gave similar fragments between im</em><em>m</em><em>obilized and without i</em><em>m</em><em>mobilized.</em><em></em></pre><pre><em> </em></pre><pre><strong><em>Keywords: </em></strong><em>Immobilization, Pseudomonas fluorescens, Carrageenan, Gel Electrophoresis.</em></pre>","PeriodicalId":32369,"journal":{"name":"Kartika Jurnal Ilmiah Farmasi","volume":"15 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ANALISIS FRAGMEN DNA DARI BAKTERI PSEUDOMONAS FLUORESCENS SEBELUM DAN SESUDAH IMOBILISASI DALAM κ-KARAGENAN\",\"authors\":\"Syarif Hamdani, Isma Oktadiana, Dewi Astriany\",\"doi\":\"10.26874/KJIF.V6I2.153\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p align=\\\"center\\\"><strong>Abstrak</strong></p><p align=\\\"center\\\"><strong> </strong></p><p>Imobilisasi merupakan pengurungan fisik atau lokalisasi bakteri utuh dalam lingkungan tertentu untuk memaksimalkan aktivitas biokatalis yang diinginkan. Imobilisasi mampu menyediakan bakteri sebagai biokatalis dalam konsentrasi tinggi sehingga dapat meningkatkan efisiensi serta produktivitas. Penelitian ini dilakukan untuk mengamati efek imobilisasi terhadap fragmen DNA bakteri setelah penyimpanan dingin selama lima bulan, bakteri yang digunakan adalah <em>Pseudomonas fluorescens</em> yang diimobilisasikan pada κ-karagenan lokal yang berasal dari perairan Karimun Jawa. Pengamatan dilakukan terhadap fragmen DNA setelah direstriksi oleh enzim EcoRI dan dianalisis menggunakan elektroforesis gel. Bakteri diimobilisasi dalam karagenan pada konsentrasi 1% dan 2% (b/v). Hasil menunjukkan bahwa DNA <em>P.</em><em>fluorescens</em> memberikan fragmen identik antara bakteri terimobilisasi dan tanpa imobilisasi.</p><p> </p><p><strong>Kata Kunci</strong> : Imobilisasi, <em>Pseudomonas fluorescens, </em>Karagenan, Elektroforesis Gel.</p><p align=\\\"center\\\"><strong> </strong></p><h2><em>Analysis of Pseudomonas fluorescens’s </em><em>DNA fragments before and after immobilize in </em><em>κ-carrageenan</em><em> </em></h2><p align=\\\"center\\\"><strong><em> </em></strong></p><p align=\\\"center\\\"><strong><em>Abstract</em></strong></p><p align=\\\"center\\\"><strong><em> </em></strong></p><pre><em>Immobilization is physical confinement or localization of bacteria in a particular environment to maximize biocatalyst activity. Immobilization provide bacteria as biocatalysts in high concentrations in order to increase efficiency and productivity. This study was conducted to observe the effect of immobilization to bacterial DNA fragments after cool storage for five months, bacteria used was Pseudomonas fluorescens that im</em><em>m</em><em>obilized in local κ-carrageenan from Karimun Jawa. Observations conducted to DNA fragments after being restricted by EcoRI enzymes and analyzed using gel electrophoresis. The bacteria were immobilized in carrageenan on concentrations of 1% and 2% (w / v), respectively. Result shown that P.fluorescens's DNA gave similar fragments between im</em><em>m</em><em>obilized and without i</em><em>m</em><em>mobilized.</em><em></em></pre><pre><em> </em></pre><pre><strong><em>Keywords: </em></strong><em>Immobilization, Pseudomonas fluorescens, Carrageenan, Gel Electrophoresis.</em></pre>\",\"PeriodicalId\":32369,\"journal\":{\"name\":\"Kartika Jurnal Ilmiah Farmasi\",\"volume\":\"15 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Kartika Jurnal Ilmiah Farmasi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26874/KJIF.V6I2.153\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kartika Jurnal Ilmiah Farmasi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26874/KJIF.V6I2.153","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
固定是在一个特定环境中对完整细菌进行物理抑制或局部化，以最大限度地提高所需的生物催化剂活性。固定可以让细菌成为高浓度的生物催化剂，从而提高效率和生产力。这个研究来观察固定效应对细菌的DNA片段的冷存储后五个月,使用的是假单胞菌细菌fluorescens diimobilisasikanκ的当地的-karagenan来自Karimun水域。对DNA片段的观察是在EcoRI酶被抑制后对其进行的，并使用电泳凝胶进行分析。细菌以1%和2%的浓度(b/v)在karagenan中活跃。结果表明，P.fluorescens的DNA提供了固定细菌和不固定细菌的相同碎片。关键词:固化、伪氟化物、碳原、电光凝胶。DNA分析fluorescens假单胞菌的fragments之前和之后immobilize inκ-carrageenan抽象Immobilization是细菌的身体刚刚禁闭或localization in a社会环境to maximize biocatalyst活动。不可移动保护细菌，如高浓度生物目录，要求增加efficiency and production。这个study was conducted天文immobilization效应》去参加DNA bacterial fragments酷存储为五个月之后,细菌fluorescens假单胞菌以前是那个immobilized in localκ-carrageenan来自Karimun。经过EcoRI enzymes对DNA片段的分析，使用电泳凝胶进行分析。细菌被抑制在1%和2% (w / v)浓度上。再生P.氟化DNA提供了类似于固化和不固化之间的碎片。Keywords:止步，伪氟化假体，Carrageenan, Gel电晕。
ANALISIS FRAGMEN DNA DARI BAKTERI PSEUDOMONAS FLUORESCENS SEBELUM DAN SESUDAH IMOBILISASI DALAM κ-KARAGENAN
Abstrak
Imobilisasi merupakan pengurungan fisik atau lokalisasi bakteri utuh dalam lingkungan tertentu untuk memaksimalkan aktivitas biokatalis yang diinginkan. Imobilisasi mampu menyediakan bakteri sebagai biokatalis dalam konsentrasi tinggi sehingga dapat meningkatkan efisiensi serta produktivitas. Penelitian ini dilakukan untuk mengamati efek imobilisasi terhadap fragmen DNA bakteri setelah penyimpanan dingin selama lima bulan, bakteri yang digunakan adalah Pseudomonas fluorescens yang diimobilisasikan pada κ-karagenan lokal yang berasal dari perairan Karimun Jawa. Pengamatan dilakukan terhadap fragmen DNA setelah direstriksi oleh enzim EcoRI dan dianalisis menggunakan elektroforesis gel. Bakteri diimobilisasi dalam karagenan pada konsentrasi 1% dan 2% (b/v). Hasil menunjukkan bahwa DNA P.fluorescens memberikan fragmen identik antara bakteri terimobilisasi dan tanpa imobilisasi.
Kata Kunci : Imobilisasi, Pseudomonas fluorescens, Karagenan, Elektroforesis Gel.
Analysis of Pseudomonas fluorescens’s DNA fragments before and after immobilize in κ-carrageenan
Abstract
Immobilization is physical confinement or localization of bacteria in a particular environment to maximize biocatalyst activity. Immobilization provide bacteria as biocatalysts in high concentrations in order to increase efficiency and productivity. This study was conducted to observe the effect of immobilization to bacterial DNA fragments after cool storage for five months, bacteria used was Pseudomonas fluorescens that immobilized in local κ-carrageenan from Karimun Jawa. Observations conducted to DNA fragments after being restricted by EcoRI enzymes and analyzed using gel electrophoresis. The bacteria were immobilized in carrageenan on concentrations of 1% and 2% (w / v), respectively. Result shown that P.fluorescens's DNA gave similar fragments between immobilized and without immobilized.
Keywords: Immobilization, Pseudomonas fluorescens, Carrageenan, Gel Electrophoresis.
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