2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)最新文献_第6页

Direct laser writing of 3D protein structures with nanoscale feature sizes 具有纳米级特征尺寸的三维蛋白质结构的直接激光书写

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765679

D. Serien, S. Takeuchi

引用次数: 1

Nanoelectroporation and controllable intracellular delivery into localized single cell with high transfection and cell viability 纳米电穿孔和可控的细胞内递送到局部单细胞，具有高转染和细胞活力

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765778

T. Santra, J. Borana, Pen-Cheng Wang, F. Tseng

{"title":"Nanoelectroporation and controllable intracellular delivery into localized single cell with high transfection and cell viability","authors":"T. Santra, J. Borana, Pen-Cheng Wang, F. Tseng","doi":"10.1109/MEMSYS.2014.6765778","DOIUrl":"https://doi.org/10.1109/MEMSYS.2014.6765778","url":null,"abstract":"Physical introduction of foreign biomolecules such as genes, proteins, DNA and RNA into living cells with high efficiency is a challenging task for biological and therapeutic research. Bulk electroporation technique, where high electric field pulses were applied to millions of cells together in-between two large electrodes, though widely employed, however is nonspecific resulting in variable efficiency with low cell viability. Here we demonstrate controllable nano-electroporation platform for HeLa cell and human Caucasian Gastric Adenocarcinoma (AGS) cell to achieve high efficient bimolecular delivery with high cell viability. Our system consists of 40nm triangular Indium Tin Oxide (ITO) metal tip with 60nm electrode gap to provide high intense electric filed into the local region of the single cell membrane. Therefore biomolecules can be delivered by much enhance electrophoresis and diffusion effects during pulsing process through a small specific nano-region of the single cell, where remaining other area of the cell membrane unaffected. This microfluidic device has great ability to offer spatial, temporal and qualitative dosage control as well as very high transfection efficiency and high cell viability.","PeriodicalId":312056,"journal":{"name":"2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)","volume":"282 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123376343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Free surface propulsion by electrowetting-assisted ‘Cheerios effect’ 电润湿辅助“Cheerios效应”的自由表面推进

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765810

Junqi Yuan, S. Cho

{"title":"Free surface propulsion by electrowetting-assisted ‘Cheerios effect’","authors":"Junqi Yuan, S. Cho","doi":"10.1109/MEMSYS.2014.6765810","DOIUrl":"https://doi.org/10.1109/MEMSYS.2014.6765810","url":null,"abstract":"This paper presents how “Cheerios effect” is combined with EWOD (electrowetting on dielectric) in order to propel floating objects. Cheerios effect is a common phenomenon in which small floating objects are either attracted or repelled by the sidewall. This behavior is known to be highly dependent on the hydrophobicity of the walls. By electrically controlling the hydrophobicity (contact angle) of the sidewalls in the canal and thus distorting the adjacent interfaces, cheerios effect can be electrically controlled to manipulate floating objects. This work first verifies EWOD-assisted cheerios effect on propulsion. Using this effect, a linear propulsion of millimeter-sized floating objects in a canal is achieved. By sequentially applying a voltage to the arrayed EWOD electrodes on the sidewalls and by carefully controlling the time interval between electrode switching, floating objects can be propelled continuously along the mini canals. In addition, the similar propulsion is achieved in an even smaller canal whose dimension is comparable to the capillary length. Finally, circular rotation of floating objects is realized by arranging EWOD electrodes along a circular path and applying electrical signal sequentially.","PeriodicalId":312056,"journal":{"name":"2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124967662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Batch release of monodisperse liposomes triggered by pulsed voltage stimulation 脉冲电压刺激触发单分散脂质体的批量释放

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765675

T. Osaki, K. Kamiya, R. Kawano, S. Takeuchi

引用次数: 3

Polymer micromachining based on Cu On Polyimide substrate and its application to flexible MEMS sensor 基于Cu on聚酰亚胺基板的聚合物微加工及其在柔性MEMS传感器中的应用

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765694

Yosuke Niimi, S. Shibata, M. Shikida

引用次数: 9

Whole-angle-mode micromachined fused-silica birdbath resonator gyroscope (WA-BRG) 全角模微加工熔融硅水盆谐振陀螺仪(WA-BRG)

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765563

J. Woo, J. Cho, C. Boyd, K. Najafi

引用次数: 35

Detection of mutations in the binding domain of tau protein by kinesin-microtubule gliding assay 激酶微管滑动法检测tau蛋白结合域突变

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765639

S. Subramaniyan, M. Tarhan, S. Karsten, H. Fujita, H. Shintaku, H. Kotera, R. Yokokawa

{"title":"Detection of mutations in the binding domain of tau protein by kinesin-microtubule gliding assay","authors":"S. Subramaniyan, M. Tarhan, S. Karsten, H. Fujita, H. Shintaku, H. Kotera, R. Yokokawa","doi":"10.1109/MEMSYS.2014.6765639","DOIUrl":"https://doi.org/10.1109/MEMSYS.2014.6765639","url":null,"abstract":"Tau protein is a biomarker for neurodegeneration. The microtubule (MT)-tau binding affinity varies according to the type of tau isoform and their degree of phosphorylation. We have utilized the difference in binding affinity of tau protein to MT to be evaluated by kinesin motor protein based MT gliding system, in order to detect and differentiate tau isoforms and their mutants. Evaluation parameters are landing rate and density of MTs on a kinesin-coated surface, and their strong correlation enables us to measure only landing rate to distinguish 2N3R and 2N4R (tau isoforms) and mutants. Secondly; we designed and fabricated a microstructure to detect tau-attached MTs, which is composed of a reservoir, parallel channels and collectors. Increase of fluorescent intensity by accumulation of MTs over time was successfully detected at the collector areas. Sensitive and rapid MT-kinesin based detection of tau isoforms (3R/4R) and mutated tau proteins on a microchip format will aid in differential diagnosis and early detection of neurodegenerative condition such as Alzheimer disease (AD).","PeriodicalId":312056,"journal":{"name":"2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121630007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Measuring flow velocity of swallowed liquid in the human pharynx by tongue pressure sensor and swallowing sound sensor 用舌压传感器和吞咽声传感器测量吞咽液体在人咽部的流速

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765774

Y. Takei, T. Kaneko, K. Noda, Kiyoshi Matsumoto, I. Shimoyama

引用次数: 2

A high performance triboelectric generator for harvesting low frequency ambient vibration energy 一种用于收集低频环境振动能量的高性能摩擦发电机

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765647

B. Meng, W. Tang, X. Zhang, M. Han, X. M. Sun, W. Liu, H. X. Zhang

引用次数: 5

3D solid-state supercapacitors obtained by ALD coating of high-density carbon nanotubes bundles 高密度碳纳米管束ALD涂层制备三维固态超级电容器

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS) Pub Date : 2014-03-13 DOI: 10.1109/MEMSYS.2014.6765646

G. Fiorentino, S. Vollebregt, F. Tichelaar, R. Ishihara, P. Sarro

引用次数: 10