Advanced Nanobiomed Research最新文献_第5页

Highly Loaded Reactive Oxygen Species–Responsive Theranostic Lenvatinib-Prodrug Nanoparticles Produced by Dispersion Polymerization 分散聚合制备的高负荷活性氧反应治疗Lenvatinib-Prodrug纳米颗粒

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-03-18 DOI: 10.1002/anbr.202400187

Sarah Spiewok, Felicitas Jansen, Jiaying Han, Markus Lamla, Max von Delius, Christian Trautwein, Laura De Laporte, Alexander J. C. Kuehne

{"title":"Highly Loaded Reactive Oxygen Species–Responsive Theranostic Lenvatinib-Prodrug Nanoparticles Produced by Dispersion Polymerization","authors":"Sarah Spiewok, Felicitas Jansen, Jiaying Han, Markus Lamla, Max von Delius, Christian Trautwein, Laura De Laporte, Alexander J. C. Kuehne","doi":"10.1002/anbr.202400187","DOIUrl":"10.1002/anbr.202400187","url":null,"abstract":"Nanoparticles represent a powerful class of materials for drug delivery, leveraging their small size for passive targeting through the enhanced permeability and retention effect in tumors. This universal approach in tumor targeting offers several advantages over free therapeutics, particularly when combined with imaging capabilities. While a plethora of nanoparticles exist for various imaging techniques, the number of nanoparticles with therapeutic functions is much smaller, due to the synthetic challenges present for incorporation and release of an active drug. Herein, a strategy to transform the tyrosine kinase inhibitor lenvatinib into a polymerizable prodrug monomer is presented, enabling its incorporation into biodegradable polyimidazole-based particles. This drug monomer is then polymerized and thus incorporated into the nanoparticles via direct arylation in a dispersion polymerization approach. The polyimidazole backbone allows for high drug loads of up to 90 wt%. Additionally, the photoacoustic properties of the polyimidazole nanoparticles are preserved after drug incorporation. Moreover, the backbone remains degradable upon exposure to hydrogen peroxide, facilitating drug release. This approach enables packaging of a drug, for which no prodrug approaches exist and which is therefore challenging to incorporate into particles due to limited functional groups. The result is a new theranostic nanoagent.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 5","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella 抑制基于蜘蛛丝涂层的骨科植入物生物膜的形成可提高黑孢Galleria mellonella的存活率

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-03-10 DOI: 10.1002/anbr.202400160

Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel

{"title":"Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella","authors":"Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel","doi":"10.1002/anbr.202400160","DOIUrl":"10.1002/anbr.202400160","url":null,"abstract":"\u0000The microbial repellence of some spider silk-based materials makes them interesting candidates for biomedical applications. This study investigates the microbial repellent properties of recombinant spider silk coatings on orthopedic metal implants, specifically targeting the prevention of biofilm-related implant infections caused by multidrug-resistant bacteria such as Staphylococcus aureus. Utilizing Galleria mellonella as an in vivo model, stainless steel and titanium implants coated with films made of three different recombinant spider silk proteins are analyzed concerning biofilm formation and its impact on animal survival. Amongst the tested spider silk variants, the polyanionic eADF4(C16) demonstrates superior bacterial-repellent properties and improved larval survivability. Scanning electron microscopy analysis reveals reduced bacterial presence on eADF4(C16)-coated wires compared to uncoated controls, correlating with survival data. Based on the results, the potential of recombinant spider silk coatings to enhance implant functionality and longevity is highlighted, presenting a novel solution to combat biofilm-related implant infections and address the growing threat of antimicrobial resistance. Furthermore, employing Galleria mellonella as an in vivo model underscores a commitment to ethical research practices in studying biofilm infections.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene Nanoparticles: Orchestrating Spherioidogenesis for Targeted Osteogenic and Neurogenic Differentiation MXene纳米颗粒：定向成骨和神经分化的球状形成

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-03-09 DOI: 10.1002/anbr.202570031

Yoonjoo Kang, Hyeongtaek Park, Surim Shim, Gul Karima, Subeen Lee, Kisuk Yang, Hwan D. Kim

引用次数: 0

Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound-Phased Array 利用超声相控阵对微尺度颗粒和活细胞进行动态可转向图案化

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-26 DOI: 10.1002/anbr.202400172

Rick J. P. van Bergen, Bart G. W. Groenen, Daniëlle C. A. Duffhues, Richard G. P. Lopata, Carlijn V. C. Bouten, Hans-Martin Schwab

{"title":"Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound-Phased Array","authors":"Rick J. P. van Bergen, Bart G. W. Groenen, Daniëlle C. A. Duffhues, Richard G. P. Lopata, Carlijn V. C. Bouten, Hans-Martin Schwab","doi":"10.1002/anbr.202400172","DOIUrl":"10.1002/anbr.202400172","url":null,"abstract":"Acoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using the forces generated in an ultrasound standing wave field. The technique has found applications in fields such as cell sorting, microfabrication, and tissue engineering. For tissue engineering, acoustic patterning enables remote cell and tissue manipulation, even in clinical settings. Conventional axial patterning strategies rely on reflector-based or dual-probe approaches, limiting their application to controlled setups incompatible with in vivo conditions. In contrast, single-sided lateral patterning approaches, exploiting the transmit beamforming capabilities and tunability of a clinical ultrasound transducer array, can bridge the gap to in vivo applications. For the first time, a clinical-phased array is used to acoustically pattern microscale particles in both axial and lateral directions, with dynamic control over pattern shape and orientation by adjusting electronic transducer delays. The data are used to validate a numerical model designed to predict acoustic forces and particle displacement in current and future experiments. Finally, acoustic patterning is successfully applied to living cells, demonstrating the potential translation of the proof of concept toward living tissues. In conclusion, clinical transducer arrays can pattern particles and living cells, augmenting patterning flexibility and advancing acoustic patterning for tissue engineering.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyurethane Nanocapsules Incorporating Epigallocatechin Gallate, A Green Tea Extract 含有没食子儿茶素没食子酸酯的聚氨酯纳米胶囊，一种绿茶提取物

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-26 DOI: 10.1002/anbr.202400204

Temitope Ale, Nhyira Ghunney, Narendra Pandala, Budd Tucker, Kassandra McFadden, Jack Hutcheson, Erin Lavik

引用次数: 0

Functional Silver-Loaded Porous Composite Scaffold for Bone Tissue Bacterial Infection 用于骨组织细菌感染的功能性载银多孔复合材料支架

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-21 DOI: 10.1002/anbr.202500004

An'nan Hu, Jian Zhou

{"title":"Functional Silver-Loaded Porous Composite Scaffold for Bone Tissue Bacterial Infection","authors":"An'nan Hu, Jian Zhou","doi":"10.1002/anbr.202500004","DOIUrl":"10.1002/anbr.202500004","url":null,"abstract":"Chronic osteomyelitis poses a significant clinical challenge in orthopedic care, contributing to substantial socioeconomic burdens. To address this issue, we engineered three-dimensional porous gelatin/β-tricalcium phosphate (β-TCP) composite scaffolds incorporating silver nanoparticles (AgNPs), designed to combine antimicrobial efficacy with osteoconductive potential. The AgNP-loaded scaffolds were synthesized and characterized. Biocompatibility and antibacterial activity were systematically evaluated. Results indicated that AgNP incorporation preserved the scaffolds’ interconnected porous architecture while improving hydrophilicity, water absorption capacity, and mechanical resilience. Cell counting kit-8 (CCK-8) assays revealed no statistically significant inhibition of cell proliferation relative to AgNP-free controls (P > 0.05), with scanning electron microscopy confirming robust cellular adhesion and proliferation. Osteogenic marker expression was markedly elevated in composite scaffolds compared to controls, with these enhancements remaining unaffected by optimal AgNP loading. Sustained Ag+ ion release persisted for six weeks, correlating with prolonged antibacterial efficacy against common pathogens. Collectively, the AgNP-loaded gelatin/β-TCP scaffolds demonstrated synergistic antibacterial activity, cytocompatibility, and osteogenic promotion. These properties position the composite as a promising biomaterial for addressing infection-related bone defects, offering a dual therapeutic strategy to mitigate microbial colonization while supporting tissue regeneration.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models 基于适应光吸收和表面积归一化模型的新型类器官生长动力学定量评估管道

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-16 DOI: 10.1002/anbr.202400138

Woojin Yang, Eva Blahusova, Reece McCoy, Róisín M. Owens, Matthias Zilbauer

引用次数: 0

The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT–βTCP Composite 3D-Printed Scaffolds Zn/Cu或Zn/Co离子的共掺入提高了PEOT/PBT -βTCP复合3d打印支架的骨再生潜力

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-16 DOI: 10.1002/anbr.202400139

Martyna Nikody, Jiaping Li, David Koper, Elizabeth R. Balmayor, Pamela Habibovic, Lorenzo Moroni

{"title":"The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT–βTCP Composite 3D-Printed Scaffolds","authors":"Martyna Nikody, Jiaping Li, David Koper, Elizabeth R. Balmayor, Pamela Habibovic, Lorenzo Moroni","doi":"10.1002/anbr.202400139","DOIUrl":"10.1002/anbr.202400139","url":null,"abstract":"Treatment of critical-sized bone defects remains challenging despite bone's regenerative capacity. Herein, a combination of a biodegradable polymer possessing bone-bonding properties with bioactive β-tricalcium phosphate (βTCP) particles coated with osteogenic (Zinc) and angiogenic (copper or cobalt) ions has been proposed. βTCP was coated with zinc and copper (Zn/Cu) or zinc and cobalt (Zn/Co) using 15 mM (low) or 45 mM (high) metallic ion solutions. Composites were obtained by a combination of the βTCP with poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymer in a 50:50 ratio. Composites were additively manufactured into 3D porous scaffolds and their osteogenic and angiogenic properties evaluated using a direct culture with human mesenchymal stromal cells (hMSCs) as well as an indirect coculture with human umbilical vein endothelial cells (HUVECs). We hypothesized that the combination of Zn/Cu or Zn/Co in the form of a coating of the βTCP particles would stimulate both osteogenic and angiogenic properties of PEOT/PBT-βTCP scaffolds. In addition, we investigated whether the resulting biomaterials influenced the paracrine function of hMSCs. Zn/Cu or Zn/Co were successfully co-incorporated into the ceramic without changing its chemistry. Scaffolds containing low concentrations of Zn/Co increased the expression of RUNX2, OCN, and OPN, while scaffolds with low concentrations of Zn/Cu enhanced the expression of ALPL. On the protein level, high Zn/Co concentrations elevated ALP and collagen production. Angiogenic properties improved with increased VEGFA expression by hMSCs and branching of tubules formed by HUVECs, particularly with low concentrations of Zn/Co. Scaffolds with high ion concentrations also increased cytokine and chemokine secretion, suggesting enhanced paracrine effects.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polarized Intestinal Cell Membrane-on-Chip for Bacterial Toxin Interaction Studies 极化肠细胞膜芯片用于细菌毒素相互作用研究

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-10 DOI: 10.1002/anbr.202400135

Reece McCoy, Jeremy Treiber, George G. Malliaras, Alberto Salleo, Róisín M. Owens

{"title":"Polarized Intestinal Cell Membrane-on-Chip for Bacterial Toxin Interaction Studies","authors":"Reece McCoy, Jeremy Treiber, George G. Malliaras, Alberto Salleo, Róisín M. Owens","doi":"10.1002/anbr.202400135","DOIUrl":"10.1002/anbr.202400135","url":null,"abstract":"\u0000The virulence of a pathogen is tied to the successful interaction between the pathogen, or its toxins, and the host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical and basolateral membrane regions with distinct functions and structural compositions. Preserving these intricacies in cell membrane-on-a-chip platforms is important for retaining physiological relevance for investigating host–pathogen interactions. Consequently, a method for obtaining distinct populations of cell membrane vesicles representing the apical and basolateral membranes is presented here, in addition to the formation of their respective supported lipid bilayers (SLBs) on PEDOT:PSS conducting polymer electrodes. The apical localization of the A metalloprotease and disintegrin (ADAM10) receptor in Caco-2 cells is shown to correlate with the increased response of the Staphylococcus aureus alpha hemolysin toxin on membrane-on-a-chip platforms compared to the basolateral membrane model where the ADAM10 receptor is absent. The interaction between SLBs and the alpha hemolysin-containing extracellular vesicles (EVs) secreted by S. aureus confirm the direct effect of toxin-containing EVs on reducing the resistance of plasma membrane. This technique could find use in quantifying relative toxicity to the cell membrane, screening for cognate receptors and inhibitors, and probing toxin mechanism of action.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eggshell-Based Unconventional Biomaterials for Medical Applications 基于蛋壳的医用非常规生物材料

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-02-07 DOI: 10.1002/anbr.202570021

Maria Eduarda Torres Gouveia, Charles Milhans, Mert Gezek, Gulden Camci-Unal

引用次数: 0