Advanced Nanobiomed Research最新文献_第5页

Monitoring of Electrophysiological Functions in Brain-on-a-Chip and Brain Organoids 监测脑芯片和脑器官组织的电生理功能

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Advanced Nanobiomed Research Pub Date : 2024-09-09 DOI: 10.1002/anbr.202470091

Jiyoung Song, Hoon Eui Jeong, Andrew Choi, Hong Nam Kim

引用次数: 0

Synergetic Enhancing Effects between Platinum Nanosensitizers and Clinically Approved Stabilizing Ligands in Proton Therapy, Causing High-Yield Double-Strand Breaks of Plasmid DNA at Relevant Dose 铂纳米敏化剂与临床批准的稳定配体在质子治疗中的协同增强效应，在相关剂量下导致质粒 DNA 的高产率双链断裂

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Advanced Nanobiomed Research Pub Date : 2024-09-06 DOI: 10.1002/anbr.202400023

Sandra Zwiehoff, Astrid Hensel, Ramin Rishmawi, Parisa Shakibaei, Carina Behrends, Katrin Hommel, Christian Bäumer, Shirley Karin Knauer, Beate Timmermann, Christoph Rehbock, Stephan Barcikowski

{"title":"Synergetic Enhancing Effects between Platinum Nanosensitizers and Clinically Approved Stabilizing Ligands in Proton Therapy, Causing High-Yield Double-Strand Breaks of Plasmid DNA at Relevant Dose","authors":"Sandra Zwiehoff, Astrid Hensel, Ramin Rishmawi, Parisa Shakibaei, Carina Behrends, Katrin Hommel, Christian Bäumer, Shirley Karin Knauer, Beate Timmermann, Christoph Rehbock, Stephan Barcikowski","doi":"10.1002/anbr.202400023","DOIUrl":"https://doi.org/10.1002/anbr.202400023","url":null,"abstract":"Proton therapy is used to eradicate tumors in sensitive areas by targeted delivery of energy. Its effectiveness can be amplified using nanoparticles (NPs) as sensitizers, due to the production of reactive oxygen species at the NP's catalytically active surface, causing the cleavage of DNA. However, the impact of stabilizing macromolecular ligands capping the particles, needed for nanosensitizer dispersion in physiological fluids, is underexplored. Herein, ligand-free colloidal platinum NPs (PtNPs) fabricated by scalable laser synthesis in liquids are used, which allows studying particle and ligand effects separately. PtNPs are incubated with stabilizing concentrations of the clinically approved ligands albumin, Tween, and polyethylene glycol, and irradiated with proton beams at clinically relevant doses (2 and 5 Gy). At these doses, plasmid DNA cleavage larger than 55% of clustered DNA damage is achieved. Bovine serum albumin, Tween, and polyethylene glycol on the NP surface work as double-strand breaks (DSB) enhancers and synergetic effects occur even at low and clinically relevant particle concentrations and irradiation doses. Here, DSB enhancement by ligand-capped PtNP even exceeds the sum of the individual ligand and particle effects. The presented fundamental correlations provide selection rules for nanosensitizer design in proton therapy.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Nanoscale Bacterial Detection Utilizing a 1DZnO Optical Nanobiosensor 利用 1DZnO 光学纳米生物传感器进行实时纳米级细菌检测

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Advanced Nanobiomed Research Pub Date : 2024-09-06 DOI: 10.1002/anbr.202400013

Rafael A. Salinas, Shirlley E. Martínez Tolibia, Andrés Galdámez-Martínez, Josué E. Romero, Laura J. García-Barrera, Abdú Orduña, Carlos David Ramos, Guillermo Santana Rodríguez, Ateet Dutt

{"title":"Real-Time Nanoscale Bacterial Detection Utilizing a 1DZnO Optical Nanobiosensor","authors":"Rafael A. Salinas, Shirlley E. Martínez Tolibia, Andrés Galdámez-Martínez, Josué E. Romero, Laura J. García-Barrera, Abdú Orduña, Carlos David Ramos, Guillermo Santana Rodríguez, Ateet Dutt","doi":"10.1002/anbr.202400013","DOIUrl":"https://doi.org/10.1002/anbr.202400013","url":null,"abstract":"One-dimensional zinc oxide nanomaterials (1DZnO) have emerged as promising, cost-effective nanoplatforms with adjustable properties suitable for electrochemical and optical biosensing applications. In this work, modifications in the inherent photoluminescent response of 1DZnO are harnessed to develop a novel immunosensor tailored for detecting enteropathogenic Escherichia coli. This nanobiosensor demonstrates a modulation in photoluminescence signal, effectively responsive to analyte concentrations ranging from 1 × 102 to 1 × 108 CFU mL−1, with direct visualization of targeted bacterial cells over 1DZnO structures through scanning electron microscopy. The conceptualization of this nanobiosensor is focused on a real-time contact strategy that can significantly reduce processing and response times for pathogen detection, prospected for emergency scenarios. With this aim, the detection process unfolds in real time, with a mere 5–10 s interaction time, corroborated by the standard polymerase chain reaction approach. This synergistic validation underscores the reliability and precision of the developed biosensor. Notably, the utility of 1DZnO nanoplatforms extends beyond the realm of enteropathogenic E. coli, as the biosensing performance exhibited here holds promise for analogous applications involving other medically pertinent pathogens. This study paves the way for the broader implementation of 1DZnO-based biosensors in medical diagnostics, offering rapid, sensitive, and real-time detection capabilities.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototactic Biohybrid Microrobot Using Peptide Nanotubes-Coated Microalgae for pH-Responsive Active Drug Delivery 利用肽纳米管包覆微藻的光触媒生物杂交微型机器人实现 pH 值响应型活性药物递送

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Advanced Nanobiomed Research Pub Date : 2024-09-03 DOI: 10.1002/anbr.202400042

Laura Ha, Hyunsik Choi, Ashmeet Singh, Bolam Kim, Byung-Kwon Kaang, You-Kwan Oh, Sei Kwang Hahn, Dong-Pyo Kim

{"title":"Phototactic Biohybrid Microrobot Using Peptide Nanotubes-Coated Microalgae for pH-Responsive Active Drug Delivery","authors":"Laura Ha, Hyunsik Choi, Ashmeet Singh, Bolam Kim, Byung-Kwon Kaang, You-Kwan Oh, Sei Kwang Hahn, Dong-Pyo Kim","doi":"10.1002/anbr.202400042","DOIUrl":"https://doi.org/10.1002/anbr.202400042","url":null,"abstract":"Despite the recent wide investigation on active cancer drug delivery, there are still strong medical unmet needs for active tumor-environment responsive cancer drug delivery in terms of spatiotemporal control. Herein, a biohybrid system of pH-responsive peptide nanotubes (PNTs)-coated microalgae for active cancer drug delivery in response to the tumor-environment is developed. The amphiphilic PNTs are effectively used to encapsulate cancer drugs and coat the living microalgae of C. reinhardtii by electrostatic interactions. The drug-loaded PNTs-based biohybrid microalgae maintain agile movement with phototaxis behavior. After in vitro characterization and cytotoxicity assessment, it is shown that the biohybrid microalgae could be phototactically localized to the cancer cells and pH-responsively disassembled to release cancer drugs in a controlled manner. Finally, with the encapsulation of paclitaxel, the statistically significant suppression of tumor growth in xenograft tumor model animals is successfully demonstrated. Taken together, the feasibility of the multifunctional microrobotic platform for advanced cancer therapy is confirmed.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of Mesoporous Silica Materials in Biological Milieu: The Gateway for Therapeutic Applications 介孔二氧化硅材料在生物环境中的降解：治疗应用之门

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Advanced Nanobiomed Research Pub Date : 2024-09-02 DOI: 10.1002/anbr.202400005

Sergio E. Moya, Raquel Ruiz Hernández, Paula C. Angelomé

{"title":"Degradation of Mesoporous Silica Materials in Biological Milieu: The Gateway for Therapeutic Applications","authors":"Sergio E. Moya, Raquel Ruiz Hernández, Paula C. Angelomé","doi":"10.1002/anbr.202400005","DOIUrl":"https://doi.org/10.1002/anbr.202400005","url":null,"abstract":"Since early developments in the field of mesoporous materials, mesoporous silica has attracted large interest in drug delivery, as they display an ordered array of pores with diameters ranging from 2 to 50 nm, which can be loaded with drugs. Mesoporous silica dissolves at physiological pH, triggering the release of loaded drugs. Several studies have focused on determining the key factors that determine the biodistribution, biocompatibility, and toxicity both in vitro or in vivo. However, in vivo studies focused on the degradation of mesoporous silica materials are very scarce, despite its relevance for drug release. In this perspective, recent works addressing mesoporous materials degradation in the context of drug delivery are discussed, first from a physicochemical point of view, and secondly in in vivo settings, in animal models that are the closest conditions to the encountered when the mesoporous materials are administered to humans. Finally, further discussion about the future directions in the design of mesoporous nanomaterials for therapy and for the study of their biological fate are presented.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyaluronic Acid Influences Amino Acid Metabolism via Differential L-Type Amino Acid Transporter 1 Expression in the U87-Malignant Glioma Cell Line 透明质酸通过 L 型氨基酸转运体 1 在 U87 恶性胶质瘤细胞系中的差异表达影响氨基酸代谢

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Advanced Nanobiomed Research Pub Date : 2024-09-02 DOI: 10.1002/anbr.202400107

Ashwin A. Bale, Swaroop Thammineni, Rohit Bhargava, Brendan Harley

{"title":"Hyaluronic Acid Influences Amino Acid Metabolism via Differential L-Type Amino Acid Transporter 1 Expression in the U87-Malignant Glioma Cell Line","authors":"Ashwin A. Bale, Swaroop Thammineni, Rohit Bhargava, Brendan Harley","doi":"10.1002/anbr.202400107","DOIUrl":"https://doi.org/10.1002/anbr.202400107","url":null,"abstract":"The glioblastoma (GBM) tumor microenvironment is heterogeneous, complex, and being increasingly understood as a significant contributor to tumor progression. In brain tumors, the extracellular matrix contains a large concentration of hyaluronic acid (HA) that makes it important to study its role in cancer progression. In particular, abnormal accumulation of HA is observed in gliomas and is often associated with poor prognosis. In addition, HA is a polymer and its molecular weight (MW) distribution may influence tumor cell activity. Herein, the influence of the MW of HA on tumor cell metabolism is evaluated. A 2D cell culture approach is used to expose the U87-MG (medium glucose [MG]) cell line to different HA MWs (10, 60, and 500 kDa) and glucose concentrations (0, 5.5, and 25 mm). Notably, it is found that HA influences GBM amino acid metabolism via reduction in LAT1 transporter protein expression. Also an influence on mitochondrial respiration levels and a difference in the accumulation of some key products of cell metabolic activity (lactic acid, glutamic acid, and succinic acid) are reported. Overall, in these results, it is indicated that HA MW can influence GBM metabolic state, with implications for cell invasion and tumor progression.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142860150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in the Development of Flexible Wearable Electrodes for Electrocardiogram Monitoring During Exercise 开发用于运动时心电图监测的柔性可穿戴电极的最新进展

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Advanced Nanobiomed Research Pub Date : 2024-08-07 DOI: 10.1002/anbr.202470081

Tae Woog Kang, Jimin Lee, Youngjin Kwon, Yoon Jae Lee, Woon-Hong Yeo

引用次数: 0

DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides 用于控制释放免疫刺激性 CpG 寡聚氧核苷酸的 DNA 耗散系统

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Advanced Nanobiomed Research Pub Date : 2024-07-30 DOI: 10.1002/anbr.202400082

Aman Ishaqat, Xiaofeng Zhang, Andreas Herrmann

{"title":"DNA Dissipative System for Controlled Release of Immunostimulatory CpG Oligodeoxynucleotides","authors":"Aman Ishaqat, Xiaofeng Zhang, Andreas Herrmann","doi":"10.1002/anbr.202400082","DOIUrl":"https://doi.org/10.1002/anbr.202400082","url":null,"abstract":"Herein, a dissipative system tailored for the controlled loading and release of CpG oligodeoxynucleotides (CpG ODNs), known for their pharmacological immunostimulatory properties, is reported. The approach involves multiple cycles of deactivation and activation of the CpG ODNs via its hybridization with a complementary fuel strand, followed by its selective release mediated by the enzymatic activity of T7 exonuclease. The autonomous and temporal behavior of this dissipative system can be tuned by three factors: the design of the fuel strand and its concentration that governs the kinetics of the forward hybridization reaction, as well as the concentration of T7 exonuclease, which regulates the backward energy dissipation reaction. Furthermore, the enzyme's tolerance toward waste accumulation is demonstrated, and the system's robust performance when utilizing various fuel strands in alternating fashion is showcased. The findings underscore the potential of this approach for precise and programmable delivery of therapeutic nucleic acids in multiple cycles, with implications for enhancing immunotherapeutic strategies in which controlled kinetics of the nucleic acid is highly desired.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring of Electrophysiological Functions in Brain-on-a-Chip and Brain Organoids 监测脑芯片和脑器官组织的电生理功能

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Advanced Nanobiomed Research Pub Date : 2024-07-23 DOI: 10.1002/anbr.202400052

Jiyoung Song, Hoon Eui Jeong, Andrew Choi, Hong Nam Kim

{"title":"Monitoring of Electrophysiological Functions in Brain-on-a-Chip and Brain Organoids","authors":"Jiyoung Song, Hoon Eui Jeong, Andrew Choi, Hong Nam Kim","doi":"10.1002/anbr.202400052","DOIUrl":"10.1002/anbr.202400052","url":null,"abstract":"Though animal models are still the gold standard for fundamental biological studies and drug evaluation for brain diseases, concerns arise from an apparent lack of reflecting the human genetics and pathophysiology. Recently, human avatars such as brain-on-a-chip and brain organoids which are generated in a 3D manner using multiple types of human-originated cells have risen as alternative testing models. Particularly in monitoring the functional neuronal cells that express action potentials in brain-on-a-chip or brain organoids, various methods of measuring their electrophysiological function have been suggested for the study of brain-related disease. Recent methodologies for analyzing the electrophysiology of different types of cells in brain-on-a-chip and brain organoids are summarized in this review. We first emphasize the inherent features of brain-on-a-chip and brain organoids from the perspective of the cell culture environment and accessibility to cells in the deep layer. The applicable monitoring techniques are then overviewed based on these features. Finally, we discuss the unmet needs for electrophysiology monitoring in advanced human brain avatar models.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 9","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141810992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postembedding Iodine Staining for Contrast-Enhanced 3D Imaging of Bone Tissue Using Focused Ion Beam-Scanning Electron Microscopy 利用聚焦离子束扫描电子显微镜对骨组织进行对比度增强型三维成像的包埋后碘染色技术

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Advanced Nanobiomed Research Pub Date : 2024-07-17 DOI: 10.1002/anbr.202400035

Mahdi Ayoubi, Richard Weinkamer, Alexander F. van Tol, Maximilian Rummler, Paul Roschger, Peter C. Brugger, Andrea Berzlanovich, Luca Bertinetti, Andreas Roschger, Peter Fratzl

{"title":"Postembedding Iodine Staining for Contrast-Enhanced 3D Imaging of Bone Tissue Using Focused Ion Beam-Scanning Electron Microscopy","authors":"Mahdi Ayoubi, Richard Weinkamer, Alexander F. van Tol, Maximilian Rummler, Paul Roschger, Peter C. Brugger, Andrea Berzlanovich, Luca Bertinetti, Andreas Roschger, Peter Fratzl","doi":"10.1002/anbr.202400035","DOIUrl":"10.1002/anbr.202400035","url":null,"abstract":"For a better understanding of living tissues and materials, it is essential to study the intricate spatial relationship between cells and their surrounding tissue on the nanoscale, with a need for 3D, high-resolution imaging techniques. In the case of bone, focused ion beam-scanning electron microscopy (FIB-SEM) operated in the backscattered electron (BSE) mode proves to be a suitable method to image mineralized areas with a nominal resolution of 5 nm. However, as clinically relevant samples are often resin-embedded, the lack of atomic number (Z) contrast makes it difficult to distinguish the embedding material from unmineralized parts of the tissue, such as osteoid, in BSE images. Staining embedded samples with iodine vapor has been shown to be effective in revealing osteoid microstructure by 2D BSE imaging. Based on this idea, an iodine (Z = 53) staining protocol is developed for 3D imaging with FIB-SEM, investigating how the amount of iodine and exposure time influences the imaging outcome. Bone samples stained with this protocol also remain compatible with confocal laser scanning microscopy to visualize the lacunocanalicular network. The proposed protocol can be applied for 3D imaging of tissues exhibiting mineralized and nonmineralized regions to study physiological and pathological biomineralization.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141829609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0