Advanced Nanobiomed Research最新文献_第10页

Biomimetic Nucleation of Manganese Oxide on Silk Fibroin Nanoparticles for Designing Raspberry-Structured Tumor Environment-Responsive Anticancer Nanocarriers 二氧化锰在丝素纳米颗粒上的仿生成核，用于设计覆盆子结构肿瘤环境响应的抗癌纳米载体

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-10-15 DOI: 10.1002/anbr.202300056

Jie Wang, Yecheng Wang, Yuping Chen, Ruyin Lv, Yanfang Yu, Junwen Wang, Qichao Cheng, Yajun Shuai, Yuyin Chen, Chuanbin Mao, Mingying Yang

{"title":"Biomimetic Nucleation of Manganese Oxide on Silk Fibroin Nanoparticles for Designing Raspberry-Structured Tumor Environment-Responsive Anticancer Nanocarriers","authors":"Jie Wang, Yecheng Wang, Yuping Chen, Ruyin Lv, Yanfang Yu, Junwen Wang, Qichao Cheng, Yajun Shuai, Yuyin Chen, Chuanbin Mao, Mingying Yang","doi":"10.1002/anbr.202300056","DOIUrl":"10.1002/anbr.202300056","url":null,"abstract":"\u0000 Bombyx mori silk fibroin is a natural biomacromolecule that can be assembled into nanoparticles. Manganese dioxide (MnO2) is responsive to tumor microenvironment (TME). Herein, SF and MnO2 is integrated to develop novel TME-responsive drug carriers. Specifically, silk fibroin nanoparticles (SF-NPs) are used as a biotemplates to regulate the nucleation and self-assembly of MnO2 for designing the complex drug delivery (SM-NPs). The SM-NPs are further modified by polyethylene glycol and folic acid to improve their stability and tumor targeting. The resultant nanocarriers (SMPF-NPs) present a raspberry-like structure with lamellar MnO2 nanoparticles coating on its surface. The SMPF-NPs show a high drug-loading capability and selectively release drugs in acidic TME. Due to the catalytic activity of MnO2, the SMPF-NPs generate high levels of oxygen under H2O2 and produce more reactive oxygen after loading Ce6. In vivo and in vitro analysis prove that SMPF-NPs can accumulate in breast tumor tissues, efficiently kill cancer cells, and destroy breast cancer tumors by a combination of chemotherapy and photodynamic therapy (PDT). Moreover, the SMPF-NPs also provide fluorescence and magnetic resonance (MR) imaging for guiding cancer therapy. These results suggest that the self-assembled SF and MnO2 nanocomplex could be a novel TME-responsive nanodrug delivery system.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135758504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orthogonal Surface-Enhanced Raman Scattering/Field-Effect Transistor Detection of Breast and Colorectal Cancer-Derived Exosomes using Graphene as a Tag-Free Diagnostic Template 使用石墨烯作为无标签诊断模板的正交表面增强拉曼散射/场效应晶体管检测乳腺癌和结直肠癌衍生外泌体

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-10-08 DOI: 10.1002/anbr.202300055

Bruno Gil, Meysam Keshavarz, Dominic Wales, Ara Darzi, Eric Yeatman

{"title":"Orthogonal Surface-Enhanced Raman Scattering/Field-Effect Transistor Detection of Breast and Colorectal Cancer-Derived Exosomes using Graphene as a Tag-Free Diagnostic Template","authors":"Bruno Gil, Meysam Keshavarz, Dominic Wales, Ara Darzi, Eric Yeatman","doi":"10.1002/anbr.202300055","DOIUrl":"10.1002/anbr.202300055","url":null,"abstract":"Cancer is one of the leading causes of high mortality worldwide, affecting most of the body organs including the breast and colon. Triple-negative breast (TNBC) and colorectal cancers (CRC) usually present the poorest clinical outcomes and lowest disease-free survival rates among the different cancer types. Novel biomarkers for early detection of TNBC and CRC have been proposed throughout the years, although the rate of success remains limited. Herein, a dual method for detection of cancer-derived exosomes based on the measurement of the Raman spectra in graphene field-effect transistors (GFETs) is proposed, thereby obtaining electrical signal metrics related to the variation of the Dirac point in graphene and optical features corresponding to representative bio-molecular cancer structures in an orthogonal way. This pioneering method and classification routine can potentially be extended to other types of cancer, thus creating a unique and universal tag-free diagnostic template for early cancer diagnostics.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135197612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Basement Membrane Mimetic Hydrogel Cooperates with Rho-Associated Protein Kinase Inhibitor to Promote the Development of Acini-Like Salivary Gland Spheroids 基膜模拟水凝胶与Rho相关蛋白激酶抑制剂合作促进腺泡蛋白样唾液腺球体的发育

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-10-08 DOI: 10.1002/anbr.202300088

Eric W. Fowler, Robert L. Witt, Xinqiao Jia

{"title":"Basement Membrane Mimetic Hydrogel Cooperates with Rho-Associated Protein Kinase Inhibitor to Promote the Development of Acini-Like Salivary Gland Spheroids","authors":"Eric W. Fowler, Robert L. Witt, Xinqiao Jia","doi":"10.1002/anbr.202300088","DOIUrl":"10.1002/anbr.202300088","url":null,"abstract":"Successful engineering of functional salivary glands necessitates the creation of cell-instructive environments for ex vivo expansion and lineage specification of primary human salivary gland stem cells (hS/PCs). Herein, basement membrane mimetic hydrogels are prepared using hyaluronic acid, cell adhesive peptides, and hyperbranched polyglycerol (HPG), with or without sulfate groups, to produce “hyperGel+” or “hyperGel”, respectively. Differential scanning fluorescence experiments confirm the ability of the sulfated HPG precursor to stabilize fibroblast growth factor 10. The hydrogels are nanoporous, cytocompatible, and cell-permissive, enabling the development of multicellular hS/PC spheroids in 14 days. The incorporation of sulfated HPG species in the hydrogel enhances cell proliferation. Culture of hS/PCs in hyperGel+ in the presence of a Rho kinase inhibitor Y-27632 (Y-27) leads to the development of spheroids with a central lumen, increases the expression of acinar marker aquaporin-3 at the transcript level (AQP3), and decreases the expression of ductal marker keratin 7 at both the transcript (KRT7) and the protein levels (K7). Reduced expression of transforming growth factor beta (TGF-β) targets SMAD2/3 is also observed in Y27-treated cultures, suggesting attenuation of TGF-β signaling. Thus, hyperGel+ cooperates with the Rho-associated protein kinase inhibitor to promote the development of lumened spheroids with enhanced expression of acinar markers.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135250739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Self-Assembled Nanoparticles Constructed from Natural Polyphenols for Intestinal Diseases 由天然多酚构建的新兴自组装纳米颗粒用于肠道疾病

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-10-08 DOI: 10.1002/anbr.202300046

Qinling Liu, Yunxiang He, Yan Fang, Yue Wu, Guidong Gong, Xiao Du, Junling Guo

{"title":"Emerging Self-Assembled Nanoparticles Constructed from Natural Polyphenols for Intestinal Diseases","authors":"Qinling Liu, Yunxiang He, Yan Fang, Yue Wu, Guidong Gong, Xiao Du, Junling Guo","doi":"10.1002/anbr.202300046","DOIUrl":"10.1002/anbr.202300046","url":null,"abstract":"Intestinal diseases like inflammatory bowel disease (IBD) and colorectal cancer originate from inflammation and disruption of mucosal barriers. Polyphenols can mitigate intestinal inflammation through antioxidant, anti-inflammatory, and microbiome modulation effects. However, the poor solubility and stability of polyphenols restrict therapeutic delivery. Self-assembly provides a nanoscale platform to overcome these limitations. Polyphenol-based nanoparticles (PNPs) are formed via coordination of polyphenols with metals like iron, copper, and zinc based on the catechol/galloyl groups. Templeted assembly with amphiphilic block copolymers can also direct polyphenol self-assembly into nanostructures. PNPs prepared by these mild, aqueous methods exhibit enhanced stability, pH-responsive disassembly, high cargo-loading capacity, and targeted accumulation in inflamed intestinal tissues. PNPs can load with hydrophobic polyphenols, drugs, genes, proteins, or probiotics and demonstrate therapeutic potential in preclinical IBD, colorectal cancer, and microbiome disorder models. Ongoing challenges include augmenting prebiotic effects, multidrug encapsulation, and engineering PNPs as biotherapeutics. Future directions involve tailored polyphenol–polymer covalent assemblies and investigating PNPs interactions with enterocytes, immune cells, and microbiota. Overall, PNPs prepared by facile self-assembly combine the bioactivities of polyphenols with advanced delivery functionality, presenting new opportunities for combination and microbiota-based therapies for complex intestinal diseases.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135251190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomaterial-Based Gene Delivery to Central Nervous System Cells for the Treatment of Spinal Cord Injury 基于生物材料的中枢神经系统细胞基因传递治疗脊髓损伤

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-09-10 DOI: 10.1002/anbr.202300030

Tara K. McGuire, Martyna Stasiewicz, Ian Woods, Adrian G. Dervan, Fergal J. O’Brien

{"title":"Biomaterial-Based Gene Delivery to Central Nervous System Cells for the Treatment of Spinal Cord Injury","authors":"Tara K. McGuire, Martyna Stasiewicz, Ian Woods, Adrian G. Dervan, Fergal J. O’Brien","doi":"10.1002/anbr.202300030","DOIUrl":"10.1002/anbr.202300030","url":null,"abstract":"Spinal cord injury (SCI) is a devastating traumatic injury often causing permanent loss of function. The challenge of treating SCI stems from the development of a complex pathophysiology at the site of injury, involving multiple biochemical cascades, widespread inflammation, blood supply interruption, inhibitory scar formation, and poor regrowth of injured axons. Clinical options are limited to surgical stabilization and attempt to ameliorate secondary damage following injury. Gene therapy has significant potential to tackle multiple aspects of SCI and improve functional outcomes. The emergence of a diverse array of biomaterial‐based nonviral nanoparticle vectors capable of delivering gene‐modifying nucleic acids offers the potential to improve the efficiency and specificity of genetic cargos for spinal cord regeneration. In this review, the progress that has been made in the field of SCI repair and the different types of nanoparticles and nucleic acid cargoes that have been used are outlined, placing a particular focus on the different cell types and pathways targeted. While many challenges remain, a perspective on the future of the field of nanoparticle‐mediated gene delivery for SCI is provided, including using biomaterial scaffolds engineered specifically for SCI to deliver gene therapeutics and the exciting opportunities that exist in the post‐COVID landscape.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136072653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of 3D-Printed Magnetic Micro-Nanorobots for Targeted Therapeutics: the State of Art 用于靶向治疗的3D打印磁性微纳机器人的开发：最新进展

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-09-08 DOI: 10.1002/anbr.202300018

Ningning Hu, Lujia Ding, Yuyi Liu, Kemin Wang, Bing Zhang, Ruixue Yin, Wenju Zhou, Zhuming Bi, Wenjun Zhang

引用次数: 0

Bioresorbable Insertion Aids for Brain Implantable Flexible Probes: A Comparative Study on Silk Fibroin, Alginate, and Disaccharides 用于脑可植入柔性探针的生物可吸收插入助剂：丝纤维蛋白、海藻酸盐和二糖的比较研究

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-09-07 DOI: 10.1002/anbr.202370091

Maria Cerezo-Sanchez, Eve McGlynn, Stefania Bartoletti, Bhavani Prasad Yalagala, Beatrice Casadei Garofani, Arianna Capodiferro, Ewan Russell, Gemma Palazzolo, Finlay Walton, Giulia Curia, Hadi Heidari

引用次数: 0

Multichamber PLGA Microparticles with Enhanced Monodispersity and Encapsulation Efficiency Fabricated by a Batch-Microfluidic Hybrid Approach 采用批处理-微流控混合方法制备具有增强单分散性和封装效率的多腔PLGA微颗粒

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-09-05 DOI: 10.1002/anbr.202300044

Sunghak Choi, Bong Su Kang, Geonjun Choi, Minsu Kang, Haena Park, Nahyun Kim, Pahn–Shick Chang, Moon Kyu Kwak, Hoon Eui Jeong, Ho-Sup Jung

{"title":"Multichamber PLGA Microparticles with Enhanced Monodispersity and Encapsulation Efficiency Fabricated by a Batch-Microfluidic Hybrid Approach","authors":"Sunghak Choi, Bong Su Kang, Geonjun Choi, Minsu Kang, Haena Park, Nahyun Kim, Pahn–Shick Chang, Moon Kyu Kwak, Hoon Eui Jeong, Ho-Sup Jung","doi":"10.1002/anbr.202300044","DOIUrl":"10.1002/anbr.202300044","url":null,"abstract":"Microparticles with multiple internal chambers hold great promise as drug delivery systems due to their ability to sustain the release of drugs with short half-lives. However, conventional batch methods used for their fabrication have limitations in terms of encapsulation efficiency and particle size distributions, while microfluidic methods suffer from low production efficiency. Herein, a batch-microfluidic hybrid method is presented for fabricating poly(DL-lactic-co-glycolic acid) (PLGA) polymeric microparticles with uniformly distributed, multiple inner microchambers. A scalable batch method is utilized for primary water-in-oil (W/O) emulsions, combined with a precise microfluidic approach for generating controlled secondary emulsions. This approach results in highly uniform PLGA microparticles with tunable size and improved encapsulation efficiency. Additionally, the effect of polydopamine-based surface hydrophilic modification of microfluidic channels on drug encapsulation efficiency is investigated, achieving an efficiency of approximately 85%. The prepared multichamber PLGA microparticles exhibit an extended-release profile without initial burst release, demonstrating their potential for sustained drug delivery in various biomedical applications.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44499267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Extracellular Vesicles from Human Skin Cells Induce Pro-β-Cell Conversions in Pancreatic Ductal Cells 人类皮肤细胞的细胞外小泡诱导胰腺导管细胞Pro - β -细胞转化

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-09-05 DOI: 10.1002/anbr.202200173

Lilibeth Ortega-Pineda, Maria Angelica Rincon-Benavides, Tatiana Z. Cuellar-Gaviria, Mia Kordowski, Elizabeth Guilfoyle, Amrita Lakshmi Anaparthi, Luke R. Lemmerman, William Lawrence, Jill L. Buss, Binbin Deng, Britani N. Blackstone, Ana Salazar-Puerta, David W. McComb, Heather Powell, Daniel Gallego-Perez, Natalia Higuita-Castro

{"title":"Engineered Extracellular Vesicles from Human Skin Cells Induce Pro-β-Cell Conversions in Pancreatic Ductal Cells","authors":"Lilibeth Ortega-Pineda, Maria Angelica Rincon-Benavides, Tatiana Z. Cuellar-Gaviria, Mia Kordowski, Elizabeth Guilfoyle, Amrita Lakshmi Anaparthi, Luke R. Lemmerman, William Lawrence, Jill L. Buss, Binbin Deng, Britani N. Blackstone, Ana Salazar-Puerta, David W. McComb, Heather Powell, Daniel Gallego-Perez, Natalia Higuita-Castro","doi":"10.1002/anbr.202200173","DOIUrl":"10.1002/anbr.202200173","url":null,"abstract":"Direct nuclear reprogramming has the potential to enable the development of β cell replacement therapies for diabetes that do not require the use of progenitor/stem cell populations. However, despite their promise, current approaches to β cell-directed reprogramming rely heavily on the use of viral vectors. Herein, the use of extracellular vesicles (EVs) derived from human dermal fibroblasts (HDFs) is explored as novel nonviral carriers of endocrine cell-patterning transcription factors, to transfect and transdifferentiate pancreatic ductal epithelial cells (PDCs) into hormone-expressing cells. Electrotransfection of HDFs with expression plasmids for Pdx1, Ngn3, and MafA (PNM) leads to the release of EVs loaded with PNM at the gene, mRNA, and protein levels. Exposing PDC cultures to PNM-loaded EVs leads to successful transfection and increases PNM expression in PDCs, which ultimately result in endocrine cell-directed conversions based on the expression of insulin/c-peptide, glucagon, and glucose transporter 2 (Glut2). These findings are further corroborated in vivo in a mouse model following intraductal injection of PNM- versus sham-loaded EVs. Collectively, these findings suggest that dermal fibroblast-derived EVs can potentially serve as a powerful platform technology for the development and deployment of nonviral reprogramming-based cell therapies for insulin-dependent diabetes.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 10","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202200173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43481835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atomically Resolved Interfacial Analysis of Bone-Like Hydroxyapatite Nanoparticles on Titanium 钛表面类骨羟基磷灰石纳米粒子的原子分辨界面分析

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-09-05 DOI: 10.1002/anbr.202300051

Gustav Eriksson, Mats Hulander, Mattias Thuvander, Martin Andersson

引用次数: 0