Advanced Nanobiomed Research最新文献_第9页

Parallelized Immunomagnetic Isolation of Basophils Directly from Whole Blood 直接从全血中平行免疫磁分离嗜碱性粒细胞

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-12-03 DOI: 10.1002/anbr.202300122

Justin Myles, Nicolas Castaño, Sungu Kim, Zhenyun Zhu, Sindy K.Y. Tang

{"title":"Parallelized Immunomagnetic Isolation of Basophils Directly from Whole Blood","authors":"Justin Myles, Nicolas Castaño, Sungu Kim, Zhenyun Zhu, Sindy K.Y. Tang","doi":"10.1002/anbr.202300122","DOIUrl":"10.1002/anbr.202300122","url":null,"abstract":"Basophils are the rarest circulating white blood cells (WBCs), but they play important roles in allergic disorders and other diseases. To enhance diagnostic capabilities, it would be desirable to isolate and analyze basophils efficiently from small blood samples. In 100 μL of whole blood, there are typically ≈103 basophils, outnumbered by ≈105 WBCs and ≈108 red blood cells (RBCs). Basophils’ low abundance has therefore presented a significant challenge in their isolation from whole blood. Conventional in-bulk basophil isolation methods require lengthy processing steps and cannot work with small volumes of blood. Herein, a parallelized integrated basophil isolation device (pi-BID) is reported for the negative immunomagnetic selection of basophils directly from four samples of 100 μL of whole blood, in parallel, within 14 min including sample preparation time. The pi-BID interfaces directly with standard sample tubes, and uses a single pressure source to drive the flow in parallel microfluidic channels. Compared with conventional in-bulk basophil isolation, the pi-BID is >3× faster, and has higher purity (≈93%) and similar recovery (≈67%). Compared with other microfluidic devices for the immunomagnetic isolation of WBC subtypes, the pi-BID achieves 10× higher enrichment of target cells from whole blood, with no prior removal of RBCs necessary.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138606266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Block Copolymer-Stabilized Metal–Organic Framework Hybrids Loading Pd Nanoparticles Enable Tumor Remission Through Near-Infrared Photothermal Therapy 嵌段共聚物稳定的金属有机框架杂化物负载钯纳米粒子，可通过近红外光热疗法缓解肿瘤症状

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-12-03 DOI: 10.1002/anbr.202300107

Shang-Wei Li, Ming-Feng Hsieh, Taehun Hong, Pengwen Chen, Kensuke Osada, Xueying Liu, Ichio Aoki, Jiashing Yu, Kevin C.-W. Wu, Horacio Cabral

{"title":"Block Copolymer-Stabilized Metal–Organic Framework Hybrids Loading Pd Nanoparticles Enable Tumor Remission Through Near-Infrared Photothermal Therapy","authors":"Shang-Wei Li, Ming-Feng Hsieh, Taehun Hong, Pengwen Chen, Kensuke Osada, Xueying Liu, Ichio Aoki, Jiashing Yu, Kevin C.-W. Wu, Horacio Cabral","doi":"10.1002/anbr.202300107","DOIUrl":"10.1002/anbr.202300107","url":null,"abstract":"Metal–organic frameworks (MOFs), such as the magnetic resonance imaging-fit MIL-100 based on Fe, are gaining significant attention as versatile theranostics with high-loading capability. Moreover, as MOFs can be engineered to target tumors, there is much interest in applying them for precise pin-point treatment of cancer. Herein, Pd nanoparticles within MIL-100(Fe) are generated to create MOFs with remarkable photothermal conversion properties for cancer therapy. The Pd-loaded MIL-100(Fe) (Pd@MIL-100(Fe)) are stabilized with biocompatible block copolymers to generate MOFs with PEGylated surfaces. This is achieved by directly mixing poly(ethylene glycol)-poly(L-aspartic acid) (PEG-p(Asp)) or dopamine-modified PEG-p(Asp) (PEG-p(Asp-Dopa)) block copolymers with the MOFs in aqueous conditions. The resulting block copolymer-stabilized MOF hybrids are stable in physiological conditions. Particularly, the Pd@MIL-100(Fe)/PEG-p(Asp-Dopa) hybrids show enhanced blood circulation and increased accumulation in B16F10 melanoma. Furthermore, when irradiated with 808 nm light, the Pd@MIL-100(Fe)/PEG-p(Asp-Dopa) hybrids rapidly increase the temperature to 50 °C, enabling tumor remission. The surface-stabilized Pd@MIL-100(Fe)/polymer hybrids open viable opportunities for innovating MOF/polymer hybrid-based approaches for drug delivery.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

It Takes Two to Tango: Controlling Human Mesenchymal Stromal Cell Response via Substrate Stiffness and Surface Topography 探戈需要两个人通过基底硬度和表面形貌控制人类间充质基质细胞反应

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-12-03 DOI: 10.1002/anbr.202300042

Sofia Ribeiro, Alexandre Watigny, Yves Bayon, Manus Biggs, Dimitrios I. Zeugolis

引用次数: 0

Emerging Sensing and In Situ Detection Technologies for the Analysis of Extracellular Vesicle miRNAs 用于分析细胞外囊泡 miRNA 的新兴传感和原位检测技术

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-12-03 DOI: 10.1002/anbr.202300067

Jixuan Han, Chen Wang, Ling Zhu, Yanlian Yang

{"title":"Emerging Sensing and In Situ Detection Technologies for the Analysis of Extracellular Vesicle miRNAs","authors":"Jixuan Han, Chen Wang, Ling Zhu, Yanlian Yang","doi":"10.1002/anbr.202300067","DOIUrl":"10.1002/anbr.202300067","url":null,"abstract":"Liquid biopsy has received increasing attention as a new disease detection modality because of its noninvasive, simple sampling, and reproducible assay advantages. Among the markers of liquid biopsy, extracellular vesicles (EVs) are considered as promising disease biomarkers because they contain a large amount of biological information and have a significant role in physiological activities. The emergence and progression of some of these diseases are associated with miRNAs carried by EVs (EV-miRNAs). Therefore, high-sensitive detection of EV-miRNAs is essential in clinical applications. A growing number of strategies, including biosensors, in situ detection methods, and microfluidics have been developed for the detection of EV-miRNA and have been applied in the diagnosis of diseases such as cancer. This review summarizes the probes, signal amplification, and detection methods for EV-miRNA detection, as well as the application of membrane fusion-based in situ detection and integrated microfluidic chips for EV-miRNA detection. The challenges of these materials and techniques in clinical diagnostic applications are also discussed.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional Nanoparticles and Nanoclusters as a Theranostics and Symptoms Disappearing Agent for Traumatic Brain Injury 作为创伤性脑损伤的治疗和症状消失剂的多功能纳米粒子和纳米团簇

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-11-28 DOI: 10.1002/anbr.202300010

Fong LaiGuan Zoey, Krishna Kanta Ghosh, Mathangi Palanivel, Balázs Gulyás, Parasuraman Padmanabhan

引用次数: 0

2D Materials for Combination Therapy to Address Challenges in the Treatment of Cancer 用于联合疗法的二维材料应对癌症治疗中的挑战

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-11-28 DOI: 10.1002/anbr.202300070

Ava Self, Megan Farell, Laximicharan Samineni, Manish Kumar, Esther W. Gomez

引用次数: 0

Enhancing the Treating Efficacy of Immunotherapy through the Restructure of Tumor Microenvironment 通过重组肿瘤微环境提高免疫疗法的疗效

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-11-28 DOI: 10.1002/anbr.202300061

Bokai Gong, Wenfeng Jia, Yang Zhou, Yanyan Xu, Ya Wei, Huile Gao

引用次数: 0

Regulation of Antigen-Specific Immunotherapy with Nanomaterials 用纳米材料调节抗原特异性免疫疗法

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-11-27 DOI: 10.1002/anbr.202300068

Weifan Ye, Yiwen Jia, Hongze Ren, Yujie Xie, Meihua Yu, Yu Chen

{"title":"Regulation of Antigen-Specific Immunotherapy with Nanomaterials","authors":"Weifan Ye, Yiwen Jia, Hongze Ren, Yujie Xie, Meihua Yu, Yu Chen","doi":"10.1002/anbr.202300068","DOIUrl":"https://doi.org/10.1002/anbr.202300068","url":null,"abstract":"Nonspecific immunotherapies often induce general immune activation or suppression. Conversely, antigen-specific immunotherapy, which refers to dampening or augmenting adaptive immunity against a disease-specific antigen, increases T-cell target specificity to pathological tissues, thereby reducing side effects on the rest of the immune system. Advances in engineering strategies for nanomaterials have enabled the feasible modulation of their physicochemical features to incorporate antigens and inherently interact with innate immune cells, which remarkably amplifies the orchestration of antigen-specific immune responses against cancer and autoimmune diseases. From this contemporary perspective, the basic principles of antigen-specific immunotherapy are briefly introduced and we elucidate how the latest nanoengineering paradigms regulate the functions of heterogeneous subsets of immune cells, such as antigen-presenting cells, B cells, and regulatory or cytotoxic T cells, promoting antigen-specific immunotherapy to treat autoimmune diseases and cancer. An outlook on prospects and remaining challenges have been discussed for, translating scientific discoveries of powerful nanomaterials into medical advances in antigen-specific immunotherapy, thus offering new treatment modalities for patients with unmet needs.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138634367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD117-Targeted Intraoperative Imaging of Gastrointestinal Stromal Tumor Using a Stem-Cell-Factor-Labeled Fluorophore 使用干细胞因子标记的荧光团对胃肠道间质瘤进行 CD117 靶向术中成像

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-11-27 DOI: 10.1002/anbr.202300063

Shinsuke Nomura, Shinya Yokomizo, Zhidong Wang, Homan Kang, Kai Bao, Chengeng Yang, Brian P. Rubin, Roderick Bronson, Satoshi Kashiwagi, Hak Soo Choi

{"title":"CD117-Targeted Intraoperative Imaging of Gastrointestinal Stromal Tumor Using a Stem-Cell-Factor-Labeled Fluorophore","authors":"Shinsuke Nomura, Shinya Yokomizo, Zhidong Wang, Homan Kang, Kai Bao, Chengeng Yang, Brian P. Rubin, Roderick Bronson, Satoshi Kashiwagi, Hak Soo Choi","doi":"10.1002/anbr.202300063","DOIUrl":"https://doi.org/10.1002/anbr.202300063","url":null,"abstract":"Complete resection without damaging the capsule is the gold-standard surgical approach for nonmetastatic gastrointestinal stromal tumors (GIST). However, accurately locating tumors during surgery is challenging because GIST is covered by normal mucosal tissue, leading to suboptimal surgeries and increased cancer recurrence rates. To enhance surgical care for GIST, a cutting-edge near-infrared (NIR) fluorescent nanoprobe is presented that enables real-time navigation of GIST by specifically targeting CD117, a protein frequently overexpressed in GIST. By attaching a zwitterionic NIR fluorophore called ZW800-1C to a CD117 ligand, stem cell factor (SCF), precise targeting is achieved while minimizing nonspecific tissue interactions. In in vitro studies, the high affinity of nanoprobe for CD117-positive GIST-T1 cell lines is demonstrated, while exhibiting no binding to CD117-negative cells or GIST-5 R cells. In a xenograft model of GIST-T1 in mice, the nanoprobe produces strong and persistent NIR signals that last over 72 h following a single intravenous injection. Moreover, the nanoprobe successfully detects spontaneous tumors in the cecum of heterozygous Kit K641E mice. In these findings, the promise of CD117-targeted molecular imaging is highlighted as an intraoperative strategy for GIST. Furthermore, this imaging approach holds potential for early diagnosis, as well as monitoring GIST prognosis before and after surgical resection.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138634365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The High Potential of ε-Poly-l-Lysine for the Development of Antimicrobial Biomaterials ε-聚赖氨酸在开发抗菌生物材料方面的巨大潜力

IF 3.4

Advanced Nanobiomed Research Pub Date : 2023-11-27 DOI: 10.1002/anbr.202300080

Eloïse Lebaudy, Chloé Guilbaud-Chéreau, Benoit Frisch, Nihal Engin Vrana, Philippe Lavalle

{"title":"The High Potential of ε-Poly-l-Lysine for the Development of Antimicrobial Biomaterials","authors":"Eloïse Lebaudy, Chloé Guilbaud-Chéreau, Benoit Frisch, Nihal Engin Vrana, Philippe Lavalle","doi":"10.1002/anbr.202300080","DOIUrl":"https://doi.org/10.1002/anbr.202300080","url":null,"abstract":"ε-poly-l-lysine (ε-PLL) is a natural polypeptide/polycation originating from bacteria. Thanks to its antifungal and antibacterial properties, it is the subject of extensive research in the food and medical industries. ε-PLL is also used to develop biomaterials in a broad range of applications, such as drug delivery, wound healing, or antimicrobial coatings. Indeed, loading ε-PLL inside nanoparticles, functionalizing implant surfaces with ε-PLL, or developing hydrogels based on reactions between ε-PLL and other polymers can improve the materials properties, leading to biocompatible, antibacterial, and antifungal systems. These characteristics are necessary not only for the development of biomaterials, for their integrity in a biological environment, but also for improving the performances of medical devices. Moreover, ε-PLL can be used as an alternative to antibiotics as its mechanism of action reduces the bacterial resistance risk compared with antibiotics. Finally, “smart” systems using ε-PLL may be developed, with controllable material degradation or drug delivery via pH or temperature variations. This review sought to gather the latest research on the development of antimicrobial biomaterials based on the ε-PLL polypeptide.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"3 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202300080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138634366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0