Advanced Nanobiomed Research最新文献

Real-Time Nanoscale Bacterial Detection Utilizing a 1DZnO Optical Nanobiosensor 利用 1DZnO 光学纳米生物传感器进行实时纳米级细菌检测

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Advanced Nanobiomed Research Pub Date : 2024-11-07 DOI: 10.1002/anbr.2024700111

Rafael A. Salinas, Shirlley E. Martínez Tolibia, Andrés Galdámez-Martínez, Josué E. Romero, Laura J. García-Barrera, Abdú Orduña, Carlos David Ramos, Guillermo Santana Rodríguez, Ateet Dutt

引用次数: 0

Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface 模拟骨软骨界面的添加剂制造支架的研究进展

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Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400059

Ivo A. O. Beeren, Pieter J. Dijkstra, Carlos Mota, Sandra Camarero-Espinosa, Matthew B. Baker, Lorenzo Moroni

{"title":"Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface","authors":"Ivo A. O. Beeren, Pieter J. Dijkstra, Carlos Mota, Sandra Camarero-Espinosa, Matthew B. Baker, Lorenzo Moroni","doi":"10.1002/anbr.202400059","DOIUrl":"https://doi.org/10.1002/anbr.202400059","url":null,"abstract":"Architectural, compositional, and mechanical gradients are present in many interfacial tissues in the body. Yet desired for regeneration, the recreation of these complex natural gradients in porous scaffolds remains a challenging task. Additive manufacturing (AM) has been highlighted as a technology to fabricate constructs to regenerate interfacial tissues. Integration of different types of gradients, which can be physical, mechanical, and/or biochemical, shows promise to control cell fate and the regeneration process in a spatial controlled manner. One of the most studied tissue interfaces is the osteochondral unit which connects cartilage to bone. This tissue is often damaged because of trauma or ageing, leading to osteoarthritis; a degenerative disease and a major cause of disability worldwide. Therefore, in view of osteochondral (OC) regeneration, a state-of-the-art overview of current approaches is presented to manufacture gradient scaffolds prepared by AM techniques. The focus is on thermoplastic, hydrogel, and hybrid scaffolds comprising gradients that induce biomimicry by their physical and biological properties. The effect of these different systems on OC tissue formation in-vitro and in-vivo is addressed. Finally, an outlook on current trends of dynamic materials is provided, including proposals on how these materials could improve the mimicry of scaffolds applied for OC regeneration.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Human Amniotic Membrane Suspension Improves the Therapeutic Effect of Mesenchymal Stem Cells on Myocardial Infarction in Rats 新型人羊膜悬浮液改善了间充质干细胞对大鼠心肌梗死的治疗效果

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Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400084

Zhaoyi Li, Meirong Zhang, Yi Wang, Yijia Li, Yi Zhun Zhu

引用次数: 0

Engineering and Monitoring the Sustained Release of Extracellular Vesicles from Hydrogels for In Vivo Therapeutic Applications 为体内治疗应用设计并监测水凝胶中细胞外囊泡的持续释放

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Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400073

Selen Uman, Noah Weingarten, Mark Helmers, Amit Iyengar, Karen L. Xu, Kendra Worthington, Danika Meldrum, Jessica Dominic, Sara Guevara-Plunkett, Alexis Schiazza, Pavan Atluri, Jason A. Burdick

{"title":"Engineering and Monitoring the Sustained Release of Extracellular Vesicles from Hydrogels for In Vivo Therapeutic Applications","authors":"Selen Uman, Noah Weingarten, Mark Helmers, Amit Iyengar, Karen L. Xu, Kendra Worthington, Danika Meldrum, Jessica Dominic, Sara Guevara-Plunkett, Alexis Schiazza, Pavan Atluri, Jason A. Burdick","doi":"10.1002/anbr.202400073","DOIUrl":"https://doi.org/10.1002/anbr.202400073","url":null,"abstract":"Extracellular vesicles (EVs) are gaining interest in regenerative medicine and biomaterials have been shown to extend EV bioavailability following delivery. Herein, the labeling of both hydrogels and EVs is reported to better understand hydrogel design for sustained EV release into tissues. Shear-thinning hydrogels are engineered using guest–host (i.e., adamantane–cyclodextrin) modifications to hyaluronic acid (GH), as well as GH hydrogels with the addition of gelatin crosslinked via transglutaminase (GH+Gel) to temporally control hydrogel properties. When labeled with a near-IR dye and injected into rat myocardial tissue, the GH+Gel hydrogel is retained (>14 days) longer than the GH hydrogel alone (≈7 days), likely due to the added gelatin network. To overcome challenges associated with common EV labeling methods, a highly versatile metabolic labeling methodology is utilized via the incorporation of N-azidoacetylmannosamine-tetraacylated during EV synthesis to introduce azide groups that can then be reacted with DBCO dyes. When injected in saline, EVs are cleared within 24 h in hearts; however, hydrogels enhance EV retention, with levels based on hydrogel degradation behavior, namely, >14 days for GH+Gel hydrogel and ≈7 days for GH hydrogel alone. These findings support the use of hydrogels in EV therapies.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laponite Nanoclay-Loaded Microgel Suspensions as Supportive Matrices for Osteogenesis 皂石纳米粘土负载微凝胶悬浮液作为骨形成的支持性基质

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Advanced Nanobiomed Research Pub Date : 2024-09-11 DOI: 10.1002/anbr.202400024

Gagan K. Jalandhra, Tzong-tyng Hung, Kristopher A. Kilian

{"title":"Laponite Nanoclay-Loaded Microgel Suspensions as Supportive Matrices for Osteogenesis","authors":"Gagan K. Jalandhra, Tzong-tyng Hung, Kristopher A. Kilian","doi":"10.1002/anbr.202400024","DOIUrl":"https://doi.org/10.1002/anbr.202400024","url":null,"abstract":"Microscale carriers have emerged as promising materials for nurturing cell growth and as delivery vehicles for regenerative therapies. Carriers based on granular hydrogels have proved advantageous, where “microgels” can be formulated to have a broad range of properties to guide the behavior of adherent cells. Herein, the fabrication of osteogenic microgel matrices through the incorporation of laponite nanoclays is demonstrated. Forming a jammed suspension provides a scaffolding where cells can adhere to the surface of the microgels, with pathways for migration and proliferation fostered by the interstitial volume. By varying the content and type of laponite—RD and XLG—the degree of osteogenesis can be tuned in embedded populations of adipose-derived stem cells. The nano- and microstructured composite materials enhance osteogenesis at the transcript and protein level, leading to increased deposition of bone minerals and an increase in the compressive modulus of the assembled scaffold. Together, these microgel suspensions are promising materials for encouraging osteogenesis with scope for delivery via injection and stabilization to bone-mimetic mechanical properties after matrix deposition.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioreactors: A Regenerative Approach to Skeletal Muscle Engineering for Repair and Replacement 生物反应器：用于修复和替代的骨骼肌工程再生方法

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Advanced Nanobiomed Research Pub Date : 2024-09-11 DOI: 10.1002/anbr.202400030

Alysha Williamson, Khashayar Khoshmanesh, Elena Pirogova, Peiqi Yang, Finn Snow, Richard Williams, Anita Quigley, Rob M. I. Kapsa

引用次数: 0

Monitoring of Electrophysiological Functions in Brain-on-a-Chip and Brain Organoids 监测脑芯片和脑器官组织的电生理功能

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Advanced Nanobiomed Research Pub Date : 2024-09-09 DOI: 10.1002/anbr.202470091

Jiyoung Song, Hoon Eui Jeong, Andrew Choi, Hong Nam Kim

引用次数: 0

Synergetic Enhancing Effects between Platinum Nanosensitizers and Clinically Approved Stabilizing Ligands in Proton Therapy, Causing High-Yield Double-Strand Breaks of Plasmid DNA at Relevant Dose 铂纳米敏化剂与临床批准的稳定配体在质子治疗中的协同增强效应，在相关剂量下导致质粒 DNA 的高产率双链断裂

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Advanced Nanobiomed Research Pub Date : 2024-09-06 DOI: 10.1002/anbr.202400023

Sandra Zwiehoff, Astrid Hensel, Ramin Rishmawi, Parisa Shakibaei, Carina Behrends, Katrin Hommel, Christian Bäumer, Shirley Karin Knauer, Beate Timmermann, Christoph Rehbock, Stephan Barcikowski

{"title":"Synergetic Enhancing Effects between Platinum Nanosensitizers and Clinically Approved Stabilizing Ligands in Proton Therapy, Causing High-Yield Double-Strand Breaks of Plasmid DNA at Relevant Dose","authors":"Sandra Zwiehoff, Astrid Hensel, Ramin Rishmawi, Parisa Shakibaei, Carina Behrends, Katrin Hommel, Christian Bäumer, Shirley Karin Knauer, Beate Timmermann, Christoph Rehbock, Stephan Barcikowski","doi":"10.1002/anbr.202400023","DOIUrl":"https://doi.org/10.1002/anbr.202400023","url":null,"abstract":"Proton therapy is used to eradicate tumors in sensitive areas by targeted delivery of energy. Its effectiveness can be amplified using nanoparticles (NPs) as sensitizers, due to the production of reactive oxygen species at the NP's catalytically active surface, causing the cleavage of DNA. However, the impact of stabilizing macromolecular ligands capping the particles, needed for nanosensitizer dispersion in physiological fluids, is underexplored. Herein, ligand-free colloidal platinum NPs (PtNPs) fabricated by scalable laser synthesis in liquids are used, which allows studying particle and ligand effects separately. PtNPs are incubated with stabilizing concentrations of the clinically approved ligands albumin, Tween, and polyethylene glycol, and irradiated with proton beams at clinically relevant doses (2 and 5 Gy). At these doses, plasmid DNA cleavage larger than 55% of clustered DNA damage is achieved. Bovine serum albumin, Tween, and polyethylene glycol on the NP surface work as double-strand breaks (DSB) enhancers and synergetic effects occur even at low and clinically relevant particle concentrations and irradiation doses. Here, DSB enhancement by ligand-capped PtNP even exceeds the sum of the individual ligand and particle effects. The presented fundamental correlations provide selection rules for nanosensitizer design in proton therapy.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Nanoscale Bacterial Detection Utilizing a 1DZnO Optical Nanobiosensor 利用 1DZnO 光学纳米生物传感器进行实时纳米级细菌检测

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Advanced Nanobiomed Research Pub Date : 2024-09-06 DOI: 10.1002/anbr.202400013

Rafael A. Salinas, Shirlley E. Martínez Tolibia, Andrés Galdámez-Martínez, Josué E. Romero, Laura J. García-Barrera, Abdú Orduña, Carlos David Ramos, Guillermo Santana Rodríguez, Ateet Dutt

{"title":"Real-Time Nanoscale Bacterial Detection Utilizing a 1DZnO Optical Nanobiosensor","authors":"Rafael A. Salinas, Shirlley E. Martínez Tolibia, Andrés Galdámez-Martínez, Josué E. Romero, Laura J. García-Barrera, Abdú Orduña, Carlos David Ramos, Guillermo Santana Rodríguez, Ateet Dutt","doi":"10.1002/anbr.202400013","DOIUrl":"https://doi.org/10.1002/anbr.202400013","url":null,"abstract":"One-dimensional zinc oxide nanomaterials (1DZnO) have emerged as promising, cost-effective nanoplatforms with adjustable properties suitable for electrochemical and optical biosensing applications. In this work, modifications in the inherent photoluminescent response of 1DZnO are harnessed to develop a novel immunosensor tailored for detecting enteropathogenic Escherichia coli. This nanobiosensor demonstrates a modulation in photoluminescence signal, effectively responsive to analyte concentrations ranging from 1 × 102 to 1 × 108 CFU mL−1, with direct visualization of targeted bacterial cells over 1DZnO structures through scanning electron microscopy. The conceptualization of this nanobiosensor is focused on a real-time contact strategy that can significantly reduce processing and response times for pathogen detection, prospected for emergency scenarios. With this aim, the detection process unfolds in real time, with a mere 5–10 s interaction time, corroborated by the standard polymerase chain reaction approach. This synergistic validation underscores the reliability and precision of the developed biosensor. Notably, the utility of 1DZnO nanoplatforms extends beyond the realm of enteropathogenic E. coli, as the biosensing performance exhibited here holds promise for analogous applications involving other medically pertinent pathogens. This study paves the way for the broader implementation of 1DZnO-based biosensors in medical diagnostics, offering rapid, sensitive, and real-time detection capabilities.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototactic Biohybrid Microrobot Using Peptide Nanotubes-Coated Microalgae for pH-Responsive Active Drug Delivery 利用肽纳米管包覆微藻的光触媒生物杂交微型机器人实现 pH 值响应型活性药物递送

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Advanced Nanobiomed Research Pub Date : 2024-09-03 DOI: 10.1002/anbr.202400042

Laura Ha, Hyunsik Choi, Ashmeet Singh, Bolam Kim, Byung-Kwon Kaang, You-Kwan Oh, Sei Kwang Hahn, Dong-Pyo Kim

{"title":"Phototactic Biohybrid Microrobot Using Peptide Nanotubes-Coated Microalgae for pH-Responsive Active Drug Delivery","authors":"Laura Ha, Hyunsik Choi, Ashmeet Singh, Bolam Kim, Byung-Kwon Kaang, You-Kwan Oh, Sei Kwang Hahn, Dong-Pyo Kim","doi":"10.1002/anbr.202400042","DOIUrl":"https://doi.org/10.1002/anbr.202400042","url":null,"abstract":"Despite the recent wide investigation on active cancer drug delivery, there are still strong medical unmet needs for active tumor-environment responsive cancer drug delivery in terms of spatiotemporal control. Herein, a biohybrid system of pH-responsive peptide nanotubes (PNTs)-coated microalgae for active cancer drug delivery in response to the tumor-environment is developed. The amphiphilic PNTs are effectively used to encapsulate cancer drugs and coat the living microalgae of C. reinhardtii by electrostatic interactions. The drug-loaded PNTs-based biohybrid microalgae maintain agile movement with phototaxis behavior. After in vitro characterization and cytotoxicity assessment, it is shown that the biohybrid microalgae could be phototactically localized to the cancer cells and pH-responsively disassembled to release cancer drugs in a controlled manner. Finally, with the encapsulation of paclitaxel, the statistically significant suppression of tumor growth in xenograft tumor model animals is successfully demonstrated. Taken together, the feasibility of the multifunctional microrobotic platform for advanced cancer therapy is confirmed.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142428886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0