Advanced Nanobiomed Research最新文献

“Spin-Rinse and Repeat”: Facile Processing to Improve Cytocompatibility of Poly(3,4-Ethyelenedioxythiophene): Polystyrene Sulfonate Composites for Bioelectronics “旋转冲洗和重复”：简单的处理，以提高细胞相容性的聚（3,4-乙炔二氧噻吩）：聚苯乙烯磺酸复合材料的生物电子学

IF 4

Advanced Nanobiomed Research Pub Date : 2025-04-13 DOI: 10.1002/anbr.202400149

Joshua Killilea, Aaron Lee, Estelle A. Cuttaz, Giovanni Carlo Miceli, Leire L. Suquia, Josef A. Goding, Christopher A. R. Chapman, Rylie A. Green

{"title":"“Spin-Rinse and Repeat”: Facile Processing to Improve Cytocompatibility of Poly(3,4-Ethyelenedioxythiophene): Polystyrene Sulfonate Composites for Bioelectronics","authors":"Joshua Killilea, Aaron Lee, Estelle A. Cuttaz, Giovanni Carlo Miceli, Leire L. Suquia, Josef A. Goding, Christopher A. R. Chapman, Rylie A. Green","doi":"10.1002/anbr.202400149","DOIUrl":"https://doi.org/10.1002/anbr.202400149","url":null,"abstract":"\u0000Conductive polymers, such as poly(3,4-ethyelenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), offer exciting prospects in organic bioelectronic applications. Translation of commercially available formulations of PEDOT:PSS is hampered by leachable contaminants. Herein, a facile processing technique utilizing differences in polymer–solvent affinity and centrifugation of poor dispersions of PEDOT:PSS to remove leachable components is presented. 1H-nuclear magnetic resonance spectroscopy of the centrifugation supernatant identifies PSS as the most abundant impurity removed. To demonstrate versatility and applicability to bioelectronic applications, centrifuged PEDOT:PSS is blended with medical grade polyurethane. Bulk composite swelling and degradation are largely unaltered, and the electrochemical performance of the composite is not significantly compromised. Marked improvement in cytocompatibility is only observed in composites fabricated using centrifugation, indicating that soluble components such as PSS contribute significantly to cytotoxicity. Removal of excess PSS improves the ability of these polymer systems to act as drug delivery vehicles as evidenced by differences in chemotherapeutic efficacy of doxorubicin following electrophoretic release. It is demonstrated that excess PSS in commercial formulations can exert significant cytotoxicity, and its reduction can produce cytocompatible PEDOT:PSS materials for use across a wide spectrum of bioelectronic applications.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the Micro-Ribonucleic Acid Profiles of Plant-Derived Extracellular Vesicles and their Potential Crosskingdom Regulation 植物源性细胞外囊泡微核糖核酸谱的鉴定及其潜在的交叉皮调控

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-30 DOI: 10.1002/anbr.202400193

Fei Wang, Junyao Deng, Shushan Mo, Jiacong Ai, Yingxian Xiao, Xiaohan Zhou, Zhenhua Li, Lanya Li

{"title":"Identification of the Micro-Ribonucleic Acid Profiles of Plant-Derived Extracellular Vesicles and their Potential Crosskingdom Regulation","authors":"Fei Wang, Junyao Deng, Shushan Mo, Jiacong Ai, Yingxian Xiao, Xiaohan Zhou, Zhenhua Li, Lanya Li","doi":"10.1002/anbr.202400193","DOIUrl":"https://doi.org/10.1002/anbr.202400193","url":null,"abstract":"Plant-derived extracellular vesicles (pEVs) are nanosized vesicles that have comparable structure and properties to EVs derived from mammalian cells. Prior studies have confirmed that pEVs have remarkable efficacy in the treatment of human diseases, such as cancer. As critical regulators of gene expression, microRNAs (miRNAs) are abundant in pEVs. However, their potential functional roles and regulatory mechanisms in mediating crosskingdom regulation of mammalian cells by pEVs remain undefined. In particular, the similarities and differences in the miRNA profiles of various pEVs in gene regulation remain elusive. Herein, pEVs are isolated from grapefruit, ginger, lemon, and grape, and small RNA (sRNA) libraries are constructed to perform sRNA sequencing. Only 15 consistently expressed miRNAs are identified in these pEVs. Furthermore, the top 20 miRNAs of each pEV are highly expressed among total miRNAs, accounting for 79.93–87.12%. Through functional annotation analysis of the miRNA target genes, these miRNAs are found to be involved in regulating the progression of human cancer and viral infection. Taken together, this study demonstrates that the miRNAs contained in the pEVs play a critical role in mediating the potential crosskingdom regulatory effects against human genes and highlights their significant potential therapeutic applications in human diseases.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of Cell Death by Combined Treatment with Photosensitizer-Chitosan Nanoparticles and the Ferroptosis Inducer RSL3 in Breast Cancer Cell Lines 光敏剂-壳聚糖纳米颗粒与铁下垂诱导剂RSL3联合作用诱导乳腺癌细胞死亡

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-23 DOI: 10.1002/anbr.202400208

Marek Feith, Saikat Das Sajib, Anne Grethe Myrann, Anders Høgset, Pablo Garrido, Alfredo Martinez, Erik Knutsen, Kirsten Sandvig, Tore Skotland, Gunhild Mari Mælandsmo, Tore-Geir Iversen

{"title":"Induction of Cell Death by Combined Treatment with Photosensitizer-Chitosan Nanoparticles and the Ferroptosis Inducer RSL3 in Breast Cancer Cell Lines","authors":"Marek Feith, Saikat Das Sajib, Anne Grethe Myrann, Anders Høgset, Pablo Garrido, Alfredo Martinez, Erik Knutsen, Kirsten Sandvig, Tore Skotland, Gunhild Mari Mælandsmo, Tore-Geir Iversen","doi":"10.1002/anbr.202400208","DOIUrl":"https://doi.org/10.1002/anbr.202400208","url":null,"abstract":"\u0000Breast cancer, a prevalent malignancy worldwide, includes the triple-negative subtype (TNBC) characterized by poor treatment outcomes. TNBC has been shown to be sensitive to ferroptotic cell death, an iron-dependent cell death mechanism involving reactive oxygen species (ROS) and lipid peroxidation. Herein, biodegradable tetraphenylchlorin-conjugated chitosan nanoparticles (TPC-CS NPs) in combination with the free ferroptosis inducer RSL3 is used in MCF7 (hormone receptor-positive, epithelial) and MDA-MB-231 (hormone receptor-negative, mesenchymal-like) breast cancer cell lines. The results show that RSL3 treatment has no cytotoxic effect in MCF7 and there is no enhanced sensitivity when combined with TPC-CS NPs, while the combination sensitizes MDA-MB-231 cells. Western blot analysis reveals that the combined treatment decreases and differently affects GPX4 levels and the ratio between the two GPX isoforms in the two cell lines. In MDA-MB-231 cells, the combined treatment shows enhanced effects on lipid peroxidation, mitochondrial potential, and basal and maximal respiration, as compared to single treatments. Finally, ferroptosis expression signatures distinguish breast cancer cell lines with an increasing score in mesenchymal-like cells. Moreover, the signatures correlate with breast cancer subtypes, exhibiting the highest scores in subtypes rich in mesenchymal-like cells, particularly basal-like and claudin-low tumors, suggesting their susceptibility to ferroptosis induction.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Loaded Reactive Oxygen Species–Responsive Theranostic Lenvatinib-Prodrug Nanoparticles Produced by Dispersion Polymerization 分散聚合制备的高负荷活性氧反应治疗Lenvatinib-Prodrug纳米颗粒

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-18 DOI: 10.1002/anbr.202400187

Sarah Spiewok, Felicitas Jansen, Jiaying Han, Markus Lamla, Max von Delius, Christian Trautwein, Laura De Laporte, Alexander J. C. Kuehne

{"title":"Highly Loaded Reactive Oxygen Species–Responsive Theranostic Lenvatinib-Prodrug Nanoparticles Produced by Dispersion Polymerization","authors":"Sarah Spiewok, Felicitas Jansen, Jiaying Han, Markus Lamla, Max von Delius, Christian Trautwein, Laura De Laporte, Alexander J. C. Kuehne","doi":"10.1002/anbr.202400187","DOIUrl":"https://doi.org/10.1002/anbr.202400187","url":null,"abstract":"Nanoparticles represent a powerful class of materials for drug delivery, leveraging their small size for passive targeting through the enhanced permeability and retention effect in tumors. This universal approach in tumor targeting offers several advantages over free therapeutics, particularly when combined with imaging capabilities. While a plethora of nanoparticles exist for various imaging techniques, the number of nanoparticles with therapeutic functions is much smaller, due to the synthetic challenges present for incorporation and release of an active drug. Herein, a strategy to transform the tyrosine kinase inhibitor lenvatinib into a polymerizable prodrug monomer is presented, enabling its incorporation into biodegradable polyimidazole-based particles. This drug monomer is then polymerized and thus incorporated into the nanoparticles via direct arylation in a dispersion polymerization approach. The polyimidazole backbone allows for high drug loads of up to 90 wt%. Additionally, the photoacoustic properties of the polyimidazole nanoparticles are preserved after drug incorporation. Moreover, the backbone remains degradable upon exposure to hydrogen peroxide, facilitating drug release. This approach enables packaging of a drug, for which no prodrug approaches exist and which is therefore challenging to incorporate into particles due to limited functional groups. The result is a new theranostic nanoagent.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 5","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143926186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella 抑制基于蜘蛛丝涂层的骨科植入物生物膜的形成可提高黑孢Galleria mellonella的存活率

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-10 DOI: 10.1002/anbr.202400160

Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel

{"title":"Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella","authors":"Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel","doi":"10.1002/anbr.202400160","DOIUrl":"https://doi.org/10.1002/anbr.202400160","url":null,"abstract":"\u0000The microbial repellence of some spider silk-based materials makes them interesting candidates for biomedical applications. This study investigates the microbial repellent properties of recombinant spider silk coatings on orthopedic metal implants, specifically targeting the prevention of biofilm-related implant infections caused by multidrug-resistant bacteria such as Staphylococcus aureus. Utilizing Galleria mellonella as an in vivo model, stainless steel and titanium implants coated with films made of three different recombinant spider silk proteins are analyzed concerning biofilm formation and its impact on animal survival. Amongst the tested spider silk variants, the polyanionic eADF4(C16) demonstrates superior bacterial-repellent properties and improved larval survivability. Scanning electron microscopy analysis reveals reduced bacterial presence on eADF4(C16)-coated wires compared to uncoated controls, correlating with survival data. Based on the results, the potential of recombinant spider silk coatings to enhance implant functionality and longevity is highlighted, presenting a novel solution to combat biofilm-related implant infections and address the growing threat of antimicrobial resistance. Furthermore, employing Galleria mellonella as an in vivo model underscores a commitment to ethical research practices in studying biofilm infections.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene Nanoparticles: Orchestrating Spherioidogenesis for Targeted Osteogenic and Neurogenic Differentiation MXene纳米颗粒：定向成骨和神经分化的球状形成

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-09 DOI: 10.1002/anbr.202570031

Yoonjoo Kang, Hyeongtaek Park, Surim Shim, Gul Karima, Subeen Lee, Kisuk Yang, Hwan D. Kim

引用次数: 0

Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound-Phased Array 利用超声相控阵对微尺度颗粒和活细胞进行动态可转向图案化

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-26 DOI: 10.1002/anbr.202400172

Rick J. P. van Bergen, Bart G. W. Groenen, Daniëlle C. A. Duffhues, Richard G. P. Lopata, Carlijn V. C. Bouten, Hans-Martin Schwab

{"title":"Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound-Phased Array","authors":"Rick J. P. van Bergen, Bart G. W. Groenen, Daniëlle C. A. Duffhues, Richard G. P. Lopata, Carlijn V. C. Bouten, Hans-Martin Schwab","doi":"10.1002/anbr.202400172","DOIUrl":"https://doi.org/10.1002/anbr.202400172","url":null,"abstract":"Acoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using the forces generated in an ultrasound standing wave field. The technique has found applications in fields such as cell sorting, microfabrication, and tissue engineering. For tissue engineering, acoustic patterning enables remote cell and tissue manipulation, even in clinical settings. Conventional axial patterning strategies rely on reflector-based or dual-probe approaches, limiting their application to controlled setups incompatible with in vivo conditions. In contrast, single-sided lateral patterning approaches, exploiting the transmit beamforming capabilities and tunability of a clinical ultrasound transducer array, can bridge the gap to in vivo applications. For the first time, a clinical-phased array is used to acoustically pattern microscale particles in both axial and lateral directions, with dynamic control over pattern shape and orientation by adjusting electronic transducer delays. The data are used to validate a numerical model designed to predict acoustic forces and particle displacement in current and future experiments. Finally, acoustic patterning is successfully applied to living cells, demonstrating the potential translation of the proof of concept toward living tissues. In conclusion, clinical transducer arrays can pattern particles and living cells, augmenting patterning flexibility and advancing acoustic patterning for tissue engineering.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyurethane Nanocapsules Incorporating Epigallocatechin Gallate, A Green Tea Extract 含有没食子儿茶素没食子酸酯的聚氨酯纳米胶囊，一种绿茶提取物

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-26 DOI: 10.1002/anbr.202400204

Temitope Ale, Nhyira Ghunney, Narendra Pandala, Budd Tucker, Kassandra McFadden, Jack Hutcheson, Erin Lavik

引用次数: 0

Novel Quantitative Assessment Pipeline of Organoid Growth Dynamics Using Adapted Light Absorption and Surface Area Normalization Models 基于适应光吸收和表面积归一化模型的新型类器官生长动力学定量评估管道

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-16 DOI: 10.1002/anbr.202400138

Woojin Yang, Eva Blahusova, Reece McCoy, Róisín M. Owens, Matthias Zilbauer

引用次数: 0

The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT–βTCP Composite 3D-Printed Scaffolds Zn/Cu或Zn/Co离子的共掺入提高了PEOT/PBT -βTCP复合3d打印支架的骨再生潜力

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-16 DOI: 10.1002/anbr.202400139

Martyna Nikody, Jiaping Li, David Koper, Elizabeth R. Balmayor, Pamela Habibovic, Lorenzo Moroni

{"title":"The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT–βTCP Composite 3D-Printed Scaffolds","authors":"Martyna Nikody, Jiaping Li, David Koper, Elizabeth R. Balmayor, Pamela Habibovic, Lorenzo Moroni","doi":"10.1002/anbr.202400139","DOIUrl":"https://doi.org/10.1002/anbr.202400139","url":null,"abstract":"Treatment of critical-sized bone defects remains challenging despite bone's regenerative capacity. Herein, a combination of a biodegradable polymer possessing bone-bonding properties with bioactive β-tricalcium phosphate (βTCP) particles coated with osteogenic (Zinc) and angiogenic (copper or cobalt) ions has been proposed. βTCP was coated with zinc and copper (Zn/Cu) or zinc and cobalt (Zn/Co) using 15 mM (low) or 45 mM (high) metallic ion solutions. Composites were obtained by a combination of the βTCP with poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymer in a 50:50 ratio. Composites were additively manufactured into 3D porous scaffolds and their osteogenic and angiogenic properties evaluated using a direct culture with human mesenchymal stromal cells (hMSCs) as well as an indirect coculture with human umbilical vein endothelial cells (HUVECs). We hypothesized that the combination of Zn/Cu or Zn/Co in the form of a coating of the βTCP particles would stimulate both osteogenic and angiogenic properties of PEOT/PBT-βTCP scaffolds. In addition, we investigated whether the resulting biomaterials influenced the paracrine function of hMSCs. Zn/Cu or Zn/Co were successfully co-incorporated into the ceramic without changing its chemistry. Scaffolds containing low concentrations of Zn/Co increased the expression of RUNX2, OCN, and OPN, while scaffolds with low concentrations of Zn/Cu enhanced the expression of ALPL. On the protein level, high Zn/Co concentrations elevated ALP and collagen production. Angiogenic properties improved with increased VEGFA expression by hMSCs and branching of tubules formed by HUVECs, particularly with low concentrations of Zn/Co. Scaffolds with high ion concentrations also increased cytokine and chemokine secretion, suggesting enhanced paracrine effects.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0