Advanced Nanobiomed Research最新文献

Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-10 DOI: 10.1002/anbr.202400160

Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel

{"title":"Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella","authors":"Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel","doi":"10.1002/anbr.202400160","DOIUrl":"https://doi.org/10.1002/anbr.202400160","url":null,"abstract":"\u0000The microbial repellence of some spider silk-based materials makes them interesting candidates for biomedical applications. This study investigates the microbial repellent properties of recombinant spider silk coatings on orthopedic metal implants, specifically targeting the prevention of biofilm-related implant infections caused by multidrug-resistant bacteria such as Staphylococcus aureus. Utilizing Galleria mellonella as an in vivo model, stainless steel and titanium implants coated with films made of three different recombinant spider silk proteins are analyzed concerning biofilm formation and its impact on animal survival. Amongst the tested spider silk variants, the polyanionic eADF4(C16) demonstrates superior bacterial-repellent properties and improved larval survivability. Scanning electron microscopy analysis reveals reduced bacterial presence on eADF4(C16)-coated wires compared to uncoated controls, correlating with survival data. Based on the results, the potential of recombinant spider silk coatings to enhance implant functionality and longevity is highlighted, presenting a novel solution to combat biofilm-related implant infections and address the growing threat of antimicrobial resistance. Furthermore, employing Galleria mellonella as an in vivo model underscores a commitment to ethical research practices in studying biofilm infections.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene Nanoparticles: Orchestrating Spherioidogenesis for Targeted Osteogenic and Neurogenic Differentiation

IF 4

Advanced Nanobiomed Research Pub Date : 2025-03-09 DOI: 10.1002/anbr.202570031

Yoonjoo Kang, Hyeongtaek Park, Surim Shim, Gul Karima, Subeen Lee, Kisuk Yang, Hwan D. Kim

引用次数: 0

Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound-Phased Array 利用超声相控阵对微尺度颗粒和活细胞进行动态可转向图案化

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-26 DOI: 10.1002/anbr.202400172

Rick J. P. van Bergen, Bart G. W. Groenen, Daniëlle C. A. Duffhues, Richard G. P. Lopata, Carlijn V. C. Bouten, Hans-Martin Schwab

{"title":"Dynamic Steerable Patterning of Microscale Particles and Living Cells Using an Ultrasound-Phased Array","authors":"Rick J. P. van Bergen, Bart G. W. Groenen, Daniëlle C. A. Duffhues, Richard G. P. Lopata, Carlijn V. C. Bouten, Hans-Martin Schwab","doi":"10.1002/anbr.202400172","DOIUrl":"https://doi.org/10.1002/anbr.202400172","url":null,"abstract":"Acoustic patterning is a noncontact method to manipulate the spatial distribution of small particles using the forces generated in an ultrasound standing wave field. The technique has found applications in fields such as cell sorting, microfabrication, and tissue engineering. For tissue engineering, acoustic patterning enables remote cell and tissue manipulation, even in clinical settings. Conventional axial patterning strategies rely on reflector-based or dual-probe approaches, limiting their application to controlled setups incompatible with in vivo conditions. In contrast, single-sided lateral patterning approaches, exploiting the transmit beamforming capabilities and tunability of a clinical ultrasound transducer array, can bridge the gap to in vivo applications. For the first time, a clinical-phased array is used to acoustically pattern microscale particles in both axial and lateral directions, with dynamic control over pattern shape and orientation by adjusting electronic transducer delays. The data are used to validate a numerical model designed to predict acoustic forces and particle displacement in current and future experiments. Finally, acoustic patterning is successfully applied to living cells, demonstrating the potential translation of the proof of concept toward living tissues. In conclusion, clinical transducer arrays can pattern particles and living cells, augmenting patterning flexibility and advancing acoustic patterning for tissue engineering.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyurethane Nanocapsules Incorporating Epigallocatechin Gallate, A Green Tea Extract

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-26 DOI: 10.1002/anbr.202400204

Temitope Ale, Nhyira Ghunney, Narendra Pandala, Budd Tucker, Kassandra McFadden, Jack Hutcheson, Erin Lavik

引用次数: 0

The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT–βTCP Composite 3D-Printed Scaffolds

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-16 DOI: 10.1002/anbr.202400139

Martyna Nikody, Jiaping Li, David Koper, Elizabeth R. Balmayor, Pamela Habibovic, Lorenzo Moroni

{"title":"The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT–βTCP Composite 3D-Printed Scaffolds","authors":"Martyna Nikody, Jiaping Li, David Koper, Elizabeth R. Balmayor, Pamela Habibovic, Lorenzo Moroni","doi":"10.1002/anbr.202400139","DOIUrl":"https://doi.org/10.1002/anbr.202400139","url":null,"abstract":"Treatment of critical-sized bone defects remains challenging despite bone's regenerative capacity. Herein, a combination of a biodegradable polymer possessing bone-bonding properties with bioactive β-tricalcium phosphate (βTCP) particles coated with osteogenic (Zinc) and angiogenic (copper or cobalt) ions has been proposed. βTCP was coated with zinc and copper (Zn/Cu) or zinc and cobalt (Zn/Co) using 15 mM (low) or 45 mM (high) metallic ion solutions. Composites were obtained by a combination of the βTCP with poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymer in a 50:50 ratio. Composites were additively manufactured into 3D porous scaffolds and their osteogenic and angiogenic properties evaluated using a direct culture with human mesenchymal stromal cells (hMSCs) as well as an indirect coculture with human umbilical vein endothelial cells (HUVECs). We hypothesized that the combination of Zn/Cu or Zn/Co in the form of a coating of the βTCP particles would stimulate both osteogenic and angiogenic properties of PEOT/PBT-βTCP scaffolds. In addition, we investigated whether the resulting biomaterials influenced the paracrine function of hMSCs. Zn/Cu or Zn/Co were successfully co-incorporated into the ceramic without changing its chemistry. Scaffolds containing low concentrations of Zn/Co increased the expression of RUNX2, OCN, and OPN, while scaffolds with low concentrations of Zn/Cu enhanced the expression of ALPL. On the protein level, high Zn/Co concentrations elevated ALP and collagen production. Angiogenic properties improved with increased VEGFA expression by hMSCs and branching of tubules formed by HUVECs, particularly with low concentrations of Zn/Co. Scaffolds with high ion concentrations also increased cytokine and chemokine secretion, suggesting enhanced paracrine effects.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polarized Intestinal Cell Membrane-on-Chip for Bacterial Toxin Interaction Studies

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-10 DOI: 10.1002/anbr.202400135

Reece McCoy, Jeremy Treiber, George G. Malliaras, Alberto Salleo, Róisín M. Owens

{"title":"Polarized Intestinal Cell Membrane-on-Chip for Bacterial Toxin Interaction Studies","authors":"Reece McCoy, Jeremy Treiber, George G. Malliaras, Alberto Salleo, Róisín M. Owens","doi":"10.1002/anbr.202400135","DOIUrl":"https://doi.org/10.1002/anbr.202400135","url":null,"abstract":"\u0000The virulence of a pathogen is tied to the successful interaction between the pathogen, or its toxins, and the host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical and basolateral membrane regions with distinct functions and structural compositions. Preserving these intricacies in cell membrane-on-a-chip platforms is important for retaining physiological relevance for investigating host–pathogen interactions. Consequently, a method for obtaining distinct populations of cell membrane vesicles representing the apical and basolateral membranes is presented here, in addition to the formation of their respective supported lipid bilayers (SLBs) on PEDOT:PSS conducting polymer electrodes. The apical localization of the A metalloprotease and disintegrin (ADAM10) receptor in Caco-2 cells is shown to correlate with the increased response of the Staphylococcus aureus alpha hemolysin toxin on membrane-on-a-chip platforms compared to the basolateral membrane model where the ADAM10 receptor is absent. The interaction between SLBs and the alpha hemolysin-containing extracellular vesicles (EVs) secreted by S. aureus confirm the direct effect of toxin-containing EVs on reducing the resistance of plasma membrane. This technique could find use in quantifying relative toxicity to the cell membrane, screening for cognate receptors and inhibitors, and probing toxin mechanism of action.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eggshell-Based Unconventional Biomaterials for Medical Applications

IF 4

Advanced Nanobiomed Research Pub Date : 2025-02-07 DOI: 10.1002/anbr.202570021

Maria Eduarda Torres Gouveia, Charles Milhans, Mert Gezek, Gulden Camci-Unal

引用次数: 0

Noninvasive Detection of Chorioretinal Hypoxia via Poly(lactic-co-glycolic acid) Nanoparticles Embedded with Purely Organic Phosphors

IF 4

Advanced Nanobiomed Research Pub Date : 2025-01-26 DOI: 10.1002/anbr.202400153

Jung-Moo Heo, Van Phuc Nguyen, Mi Zheng, Jihyun Park, Yannis M. Paulus, Jinsang Kim

{"title":"Noninvasive Detection of Chorioretinal Hypoxia via Poly(lactic-co-glycolic acid) Nanoparticles Embedded with Purely Organic Phosphors","authors":"Jung-Moo Heo, Van Phuc Nguyen, Mi Zheng, Jihyun Park, Yannis M. Paulus, Jinsang Kim","doi":"10.1002/anbr.202400153","DOIUrl":"https://doi.org/10.1002/anbr.202400153","url":null,"abstract":"Ischemia-induced hypoxia is a critical complication in retinal diseases, leading to significant vision impairment and blindness due to disrupted blood flow and oxygen delivery. Currently, there is no effective method to assess oxygen levels in extravascular retinal tissue. Traditional hypoxia detection methods, such as oxygen-sensitive microelectrodes, magnetic resonance imaging, and retinal oximetry, have limitations including invasiveness, low spatial resolution, and lack of real-time monitoring. Herein, a noninvasive hypoxia detection method is proposed by utilizing lipid-polymer nanoparticles (NPs) with purely organic room-temperature phosphorescence materials for real-time detection with high spatial and temporal resolution. To enhance biocompatibility and efficacy, NPs were fabricated using biodegradable poly(lactic-co-glycolic acid) (PLGA) and SeCO as a phosphor. PLGA degrades into nontoxic by-products, while the excitation wavelength of SeCO at 393 nm minimizes damage from short wavelengths and enhances tissue penetration. Furthermore, the NPs’ size is optimized to improve cellular uptake and reduce bodily accumulation, as smaller NPs are preferred for biocompatibility. Herein, synthesis, characterization, and evaluation of these PLGA-based phosphorescent NPs in rabbit models of retinal vein occlusion and choroidal vascular occlusion are involved. This approach represents a significant advancement in noninvasive biomedical imaging, improving the diagnosis and management of ischemic retinal diseases.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Term Neural Recording Performance of PEDOT/CNT/Dexamethasone-Coated Electrode Array Implanted in Visual Cortex of Rats

IF 4

Advanced Nanobiomed Research Pub Date : 2025-01-25 DOI: 10.1002/anbr.202400114

Asiyeh Golabchi, Bingchen Wu, Zhanhong Jeff Du, Xinyan Tracy Cui

{"title":"Long-Term Neural Recording Performance of PEDOT/CNT/Dexamethasone-Coated Electrode Array Implanted in Visual Cortex of Rats","authors":"Asiyeh Golabchi, Bingchen Wu, Zhanhong Jeff Du, Xinyan Tracy Cui","doi":"10.1002/anbr.202400114","DOIUrl":"https://doi.org/10.1002/anbr.202400114","url":null,"abstract":"Implantable neural electrode arrays can be inserted in the brain to provide single-cell electrophysiology recording for neuroscience research and brain–machine interface applications. However, maintaining signal quality over time is complicated by inflammatory tissue responses and degradation of electrode materials. Organic electrode coatings offer a solution by enhancing recording and stimulation capabilities, including reduced impedance, increased charge injection capacity, and the ability to incorporate and release anti-inflammatory drugs. Herein, acid-functionalized multiwalled carbon nanotubes (CNTs) loaded with dexamethasone (Dex) are incorporated into poly(3,4-ethylendioxythiophene) (PEDOT) as electrode coatings. The electrochemical stability and recording performance of the PEDOT/CNT/Dex coating over an extended period of ≈18 months are investigated. Cyclic voltammetry (CV) stimulation is used to release Dex in half of the recording sites during the first 11 days of implantation to reduce the acute inflammation. The PEDOT/CNT/Dex-coated floating microelectrode arrays demonstrate stable in vivo electrode impedance and successful detection of visually evoked neural activity from the rat visual cortex even at chronic time points. Additionally, the CV-stimulated sites exhibit higher single-unit (SU) recording yield, amplitudes, and signal-to-noise ratio compared to unstimulated sites. These results highlight the potential of anti-inflammatory treatments to improve the quality and longevity of chronic neural recordings.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotweezers for Manipulating Untethered Micro/Nanoscale Bio-Tools: Principles, Performance, and Highlighted Applications

IF 4

Advanced Nanobiomed Research Pub Date : 2025-01-24 DOI: 10.1002/anbr.202400130

Kelly Shih, Niam Zaidi, Seung Ho Lee, Huaizhi Li, Donglei Emma Fan

引用次数: 0