Samad Mussa Farkhani, David Rudd, Helmut Thissen, Muhammad A. Ali, Mehmet Yuce, Anna Cifuentes-Rius, Nicolas H. Voelcker
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引用次数: 0
Abstract
Gold nanoclusters (AuNCs), known for their biocompatibility, intrinsic fluorescence, and magnetic properties represent an interesting nanomaterial for targeted drug delivery. Herein, a multifunctional platform is designed based on AuNCs modified with DNA strands for the loading and triggered release of doxorubicin (Dox), a chemotherapeutic agent. The surface of these nanoclusters is initially modified with a DNA strand and subsequently hybridized with a complementary DNA strand functionalized with folic acid (FA). The modification with FA facilitates targeted drug delivery to MCF-7 cells. The DNA on the AuNCs surface allows for the capture of Dox via intercalation. Cellular uptake and cytotoxicity are assessed in 2D cell culture and spheroid models. The results demonstrate a significantly higher uptake of the targeted AuNCs into MCF-7 cells compared to nontargeted counterparts. Moreover, under radiofrequency (RF) irradiation, the targeted AuNCs exhibit increased cytotoxicity. This cytotoxicity can be attributed to multiple factors, including hyperthermia induced by RF irradiation, heat-triggered release of the loaded drug, and the generation of reactive oxygen species (ROS). This research sheds light on the promising applications of AuNCs in cancer therapy, leveraging their unique properties for precise and effective treatment strategies.
期刊介绍：
Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science.
The scope of Advanced NanoBiomed Research will cover the following key subject areas:
▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging.
▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications.
▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture.
▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs.
▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization.
▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems.
with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.
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