Advanced Nanobiomed Research最新文献_第2页

Polarized Intestinal Cell Membrane-on-Chip for Bacterial Toxin Interaction Studies 极化肠细胞膜芯片用于细菌毒素相互作用研究

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Advanced Nanobiomed Research Pub Date : 2025-02-10 DOI: 10.1002/anbr.202400135

Reece McCoy, Jeremy Treiber, George G. Malliaras, Alberto Salleo, Róisín M. Owens

{"title":"Polarized Intestinal Cell Membrane-on-Chip for Bacterial Toxin Interaction Studies","authors":"Reece McCoy, Jeremy Treiber, George G. Malliaras, Alberto Salleo, Róisín M. Owens","doi":"10.1002/anbr.202400135","DOIUrl":"https://doi.org/10.1002/anbr.202400135","url":null,"abstract":"\u0000The virulence of a pathogen is tied to the successful interaction between the pathogen, or its toxins, and the host cell. Polarized epithelial cells, constituting highly specialized cell monolayers, possess apical and basolateral membrane regions with distinct functions and structural compositions. Preserving these intricacies in cell membrane-on-a-chip platforms is important for retaining physiological relevance for investigating host–pathogen interactions. Consequently, a method for obtaining distinct populations of cell membrane vesicles representing the apical and basolateral membranes is presented here, in addition to the formation of their respective supported lipid bilayers (SLBs) on PEDOT:PSS conducting polymer electrodes. The apical localization of the A metalloprotease and disintegrin (ADAM10) receptor in Caco-2 cells is shown to correlate with the increased response of the Staphylococcus aureus alpha hemolysin toxin on membrane-on-a-chip platforms compared to the basolateral membrane model where the ADAM10 receptor is absent. The interaction between SLBs and the alpha hemolysin-containing extracellular vesicles (EVs) secreted by S. aureus confirm the direct effect of toxin-containing EVs on reducing the resistance of plasma membrane. This technique could find use in quantifying relative toxicity to the cell membrane, screening for cognate receptors and inhibitors, and probing toxin mechanism of action.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eggshell-Based Unconventional Biomaterials for Medical Applications 基于蛋壳的医用非常规生物材料

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Advanced Nanobiomed Research Pub Date : 2025-02-07 DOI: 10.1002/anbr.202570021

Maria Eduarda Torres Gouveia, Charles Milhans, Mert Gezek, Gulden Camci-Unal

引用次数: 0

Noninvasive Detection of Chorioretinal Hypoxia via Poly(lactic-co-glycolic acid) Nanoparticles Embedded with Purely Organic Phosphors 聚乳酸-羟基乙酸纳米颗粒包埋纯有机荧光粉无创检测视网膜缺氧

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Advanced Nanobiomed Research Pub Date : 2025-01-26 DOI: 10.1002/anbr.202400153

Jung-Moo Heo, Van Phuc Nguyen, Mi Zheng, Jihyun Park, Yannis M. Paulus, Jinsang Kim

{"title":"Noninvasive Detection of Chorioretinal Hypoxia via Poly(lactic-co-glycolic acid) Nanoparticles Embedded with Purely Organic Phosphors","authors":"Jung-Moo Heo, Van Phuc Nguyen, Mi Zheng, Jihyun Park, Yannis M. Paulus, Jinsang Kim","doi":"10.1002/anbr.202400153","DOIUrl":"https://doi.org/10.1002/anbr.202400153","url":null,"abstract":"Ischemia-induced hypoxia is a critical complication in retinal diseases, leading to significant vision impairment and blindness due to disrupted blood flow and oxygen delivery. Currently, there is no effective method to assess oxygen levels in extravascular retinal tissue. Traditional hypoxia detection methods, such as oxygen-sensitive microelectrodes, magnetic resonance imaging, and retinal oximetry, have limitations including invasiveness, low spatial resolution, and lack of real-time monitoring. Herein, a noninvasive hypoxia detection method is proposed by utilizing lipid-polymer nanoparticles (NPs) with purely organic room-temperature phosphorescence materials for real-time detection with high spatial and temporal resolution. To enhance biocompatibility and efficacy, NPs were fabricated using biodegradable poly(lactic-co-glycolic acid) (PLGA) and SeCO as a phosphor. PLGA degrades into nontoxic by-products, while the excitation wavelength of SeCO at 393 nm minimizes damage from short wavelengths and enhances tissue penetration. Furthermore, the NPs’ size is optimized to improve cellular uptake and reduce bodily accumulation, as smaller NPs are preferred for biocompatibility. Herein, synthesis, characterization, and evaluation of these PLGA-based phosphorescent NPs in rabbit models of retinal vein occlusion and choroidal vascular occlusion are involved. This approach represents a significant advancement in noninvasive biomedical imaging, improving the diagnosis and management of ischemic retinal diseases.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Term Neural Recording Performance of PEDOT/CNT/Dexamethasone-Coated Electrode Array Implanted in Visual Cortex of Rats PEDOT/CNT/地塞米松包被电极阵列植入大鼠视觉皮层的长期神经记录性能

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Advanced Nanobiomed Research Pub Date : 2025-01-25 DOI: 10.1002/anbr.202400114

Asiyeh Golabchi, Bingchen Wu, Zhanhong Jeff Du, Xinyan Tracy Cui

{"title":"Long-Term Neural Recording Performance of PEDOT/CNT/Dexamethasone-Coated Electrode Array Implanted in Visual Cortex of Rats","authors":"Asiyeh Golabchi, Bingchen Wu, Zhanhong Jeff Du, Xinyan Tracy Cui","doi":"10.1002/anbr.202400114","DOIUrl":"https://doi.org/10.1002/anbr.202400114","url":null,"abstract":"Implantable neural electrode arrays can be inserted in the brain to provide single-cell electrophysiology recording for neuroscience research and brain–machine interface applications. However, maintaining signal quality over time is complicated by inflammatory tissue responses and degradation of electrode materials. Organic electrode coatings offer a solution by enhancing recording and stimulation capabilities, including reduced impedance, increased charge injection capacity, and the ability to incorporate and release anti-inflammatory drugs. Herein, acid-functionalized multiwalled carbon nanotubes (CNTs) loaded with dexamethasone (Dex) are incorporated into poly(3,4-ethylendioxythiophene) (PEDOT) as electrode coatings. The electrochemical stability and recording performance of the PEDOT/CNT/Dex coating over an extended period of ≈18 months are investigated. Cyclic voltammetry (CV) stimulation is used to release Dex in half of the recording sites during the first 11 days of implantation to reduce the acute inflammation. The PEDOT/CNT/Dex-coated floating microelectrode arrays demonstrate stable in vivo electrode impedance and successful detection of visually evoked neural activity from the rat visual cortex even at chronic time points. Additionally, the CV-stimulated sites exhibit higher single-unit (SU) recording yield, amplitudes, and signal-to-noise ratio compared to unstimulated sites. These results highlight the potential of anti-inflammatory treatments to improve the quality and longevity of chronic neural recordings.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotweezers for Manipulating Untethered Micro/Nanoscale Bio-Tools: Principles, Performance, and Highlighted Applications 操纵无系绳微/纳米尺度生物工具的纳米镊子：原理，性能和突出的应用

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Advanced Nanobiomed Research Pub Date : 2025-01-24 DOI: 10.1002/anbr.202400130

Kelly Shih, Niam Zaidi, Seung Ho Lee, Huaizhi Li, Donglei Emma Fan

引用次数: 0

Nanoplasmonics for Enhanced Fluorescence Detection of Nucleic Acids: From Fundamentals to Boosting Cancer Management 纳米等离子体增强核酸荧光检测：从基础到促进癌症管理

IF 4

Advanced Nanobiomed Research Pub Date : 2025-01-23 DOI: 10.1002/anbr.202400088

David Botequim, Rui Oliveira-Silva, Vanda Vaz Serra, Peter Zijlstra, Duarte Miguel de França Prazeres, Pedro Miguel Ribeiro Paulo

{"title":"Nanoplasmonics for Enhanced Fluorescence Detection of Nucleic Acids: From Fundamentals to Boosting Cancer Management","authors":"David Botequim, Rui Oliveira-Silva, Vanda Vaz Serra, Peter Zijlstra, Duarte Miguel de França Prazeres, Pedro Miguel Ribeiro Paulo","doi":"10.1002/anbr.202400088","DOIUrl":"https://doi.org/10.1002/anbr.202400088","url":null,"abstract":"Cancer remains a leading cause of mortality and morbidity worldwide. Consequently, the scientific community continues to pursue improvements in diagnostic methods and subsequent treatments. To enhance treatment efficacy and reduce the probability of adverse outcomes, reliable and sensitive diagnostic methods are essential. A potential solution may lie in the synergy between nanotechnology and optical biosensors, as they can provide exceptional sensitivity in the detection of disease biomarkers. This review focuses on fluorescent DNA probes assembled onto metal nanoparticles for cancer-related applications. These hybrid biosensors exhibit remarkable and versatile optical properties enabling to enhance signal emission by orders of magnitude. In these configurations, metallic particles function as optical antennas for fluorescent dyes, significantly increasing their photon emission rates. This review presents a novel perspective on recent advancements in cancer diagnostics and treatment utilizing hybrid biosensors. Current methodologies for cancer diagnostics are examined, toward elucidating their advantages and limitations. The subject matter is critically evaluated, and fundamental concepts are explored to assess the most promising avenues for clinical applications. These biosensors may potentially integrate into medical practice and healthcare in the near future, both for the diagnosis and prognosis of cancer, as well as for precision (P4) medicine.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrodynamic Cavitation-Induced Thrombolysis on a Clot-on-a-Chip Platform 基于芯片上凝块平台的流体动力空化诱导溶栓

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Advanced Nanobiomed Research Pub Date : 2025-01-07 DOI: 10.1002/anbr.202570011

Beyzanur Ozogul, Unal Akar, Rabia Mercimek, Farzad Rokhsar Talabazar, Seyedali Seyedmirzaei Sarraf, Araz Sheibani Aghdam, Ali Ansari Hamedani, Luis Guillermo Villanueva, Dmitry Grishenkov, Ehsan Amani, Tugrul Elverdi, Morteza Ghorbani, Ali Koşar

引用次数: 0

A State-of-the-Art Appraisal of Nanomaterials in Microneedles in the Delivery of Therapeutics and Vaccines 微针中纳米材料在治疗药物和疫苗递送中的最新评价

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Advanced Nanobiomed Research Pub Date : 2024-12-26 DOI: 10.1002/anbr.202400162

Bwalya Angel Witika, Aaron Hutton, Pedzisai A. Makoni, Ryan F. Donnelly

引用次数: 0

Detection of Estrogen Receptor Status in Breast Cancer Cytology Samples by an Optical Nanosensor 光学纳米传感器检测乳腺癌细胞学样本中雌激素受体状态

IF 4

Advanced Nanobiomed Research Pub Date : 2024-12-13 DOI: 10.1002/anbr.202400099

Pooja V. Gaikwad, Nazifa Rahman, Pratyusha Ghosh, Dianna L. Ng, Ryan M. Williams

{"title":"Detection of Estrogen Receptor Status in Breast Cancer Cytology Samples by an Optical Nanosensor","authors":"Pooja V. Gaikwad, Nazifa Rahman, Pratyusha Ghosh, Dianna L. Ng, Ryan M. Williams","doi":"10.1002/anbr.202400099","DOIUrl":"https://doi.org/10.1002/anbr.202400099","url":null,"abstract":"Breast cancer is a substantial source of morbidity and mortality worldwide. Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) are the primary biomarkers which inform breast cancer treatment. Although endocrine therapy for ER+ patients is widely available, there is a need for increased access to low-cost, rapid, and accurate ER testing methods. In this work, we designed a near-infrared optical nanosensor using single-walled carbon nanotubes (SWCNT) as the transducer and an anti-ERα antibody as the recognition element. We evaluated the nanosensor in vitro prior to testing with 26 breast cancer samples which were collected by scraping the cut surface of fresh, surgically resected tumors. Twenty samples were ER+, and six ER−, representing 13 unique patients. We found that the nanosensor can differentiate ER− from ER+ patient biopsies through a shift in its center wavelength upon sample addition. Receiver operating characteristic area under the curve analyses determined that the strongest classifier with an AUC of 0.94 was the (7,5) SWCNT after direct incubation and measurement, and without further processing. We anticipate that further testing and development of this nanosensor may push its utility toward field-deployable, rapid ER subtyping with the potential for additional molecular marker profiling.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143114665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soft Cardiac Patch Using a Bifacial Architecture of Adhesive/Low-Impedance Hydrogel Nanocomposites and Highly Conductive Elastomer Nanocomposites 基于粘合剂/低阻抗水凝胶纳米复合材料和高导电性弹性体纳米复合材料双面结构的软心脏贴片

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Advanced Nanobiomed Research Pub Date : 2024-12-13 DOI: 10.1002/anbr.202400143

Jeeyoung Kim, Gi Doo Cha, Minsung Kim, Seung-Pyo Lee, Sung-Hyuk Sunwoo, Dae-Hyeong Kim

{"title":"Soft Cardiac Patch Using a Bifacial Architecture of Adhesive/Low-Impedance Hydrogel Nanocomposites and Highly Conductive Elastomer Nanocomposites","authors":"Jeeyoung Kim, Gi Doo Cha, Minsung Kim, Seung-Pyo Lee, Sung-Hyuk Sunwoo, Dae-Hyeong Kim","doi":"10.1002/anbr.202400143","DOIUrl":"https://doi.org/10.1002/anbr.202400143","url":null,"abstract":"\u0000Soft implantable multichannel cardiac electrode arrays that establish direct monolithic interfaces with the heart are key components for advanced cardiac monitoring and electrical modulation. A significant technological advancement in this area is the development of stretchable conductive nanocomposites, fabricated through the integration of metallic nanomaterials and elastic polymers, aimed at achieving both high electrical conductivity and mechanical elasticity. Despite these advances, further progress in material performance and device designs is required to ensure seamless, reliable, biocompatible, and high-fidelity cardiac interfacing. Herein, the development of a soft multichannel cardiac patch based on a bifacial architecture of adhesive/low-impedance hydrogel nanocomposites and highly conductive elastomer nanocomposites is reported. The bifacial design facilitates the integration of the cardiac patch between the heart and other tissues/organs can be achieved. The hydrogel nanocomposite layer, positioned on the epicardial side, provides stable adhesion to the target cardiac tissue and enables low-impedance biocompatible interfacing with the heart, while the elastomer nanocomposite layer, positioned on the opposite side, offers high electrical conductivity for facile electrophysiological signal transfer and a low-friction surface minimizing unwanted interactions with surrounding tissues. The effectiveness of this bifacial patch in multiple applications involving various cardiac signal recordings and electromechanical modulation demonstrations is showcased.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0