Advanced Nanobiomed Research最新文献_第2页

Spatial Cell Death and Oxidative Stress Dynamics in Gas Plasma-Treated Tumor Tissues 气体等离子体治疗肿瘤组织的空间细胞死亡和氧化应激动力学

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-31 Epub Date: 2026-05-01 DOI: 10.1002/anbr.202500291

Anke Schmidt, Shahrzad Rokhsary, Linus Hübner, Lea Miebach, Sander Bekeschus

{"title":"Spatial Cell Death and Oxidative Stress Dynamics in Gas Plasma-Treated Tumor Tissues","authors":"Anke Schmidt, Shahrzad Rokhsary, Linus Hübner, Lea Miebach, Sander Bekeschus","doi":"10.1002/anbr.202500291","DOIUrl":"https://doi.org/10.1002/anbr.202500291","url":null,"abstract":"Cold physical gas plasma generates various reactive oxygen and nitrogen species and has emerged as potential anticancer treatment. However, its effects on and into tumor tissue are poorly understood. Using three gas plasma-treated tumor models (in ovo Panc1 pancreas carcinoma tissue, ex vivo CT26 colorectal carcinoma tissue, and in vivo SSC7 squamous carcinoma tissue), apoptosis and tissue penetration, along with stress markers, were analyzed. Across all models, a significant increase in TUNEL-positive cells was revealed. The extent of apoptosis correlated with the intensity and duration of the gas plasma application, being most pronounced within the tumor periphery. Immunofluorescence quantification of oxidative and nitrosative stress markers, including 8-hydroxy-2′-deoxyguanosine (8-OHdG) and 3-nitrotyrosine (3-NT), showed elevated staining and significant intensity increases. This was particularly notable within the first 500 µm from the tissue surface. Finally, safety assessments were performed based on histopathological analyses of nontumor tissues in a healthy gas plasma-treated murine skin. While apoptotic events were found to some degree, no significant adverse effects on adjacent healthy tissue, such as necrosis or off-target tissue damage, were observed within the assessed zones following gas plasma exposure. These findings support a favorable safety profile under the tested conditions, which is a key consideration for future clinical translation.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148859939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Aging of Biomaterials in Ambient and Physiological Environments 生物材料在环境和生理环境中的自然老化

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-31 Epub Date: 2026-04-21 DOI: 10.1002/anbr.202500198

Shuyu Zhang, Anne E. Staples

引用次数: 0

Tunable Regional Targeting of Self-Assembling Peptide Nanomaterials in Acute Myocardial Infarction 自组装肽纳米材料在急性心肌梗死中的可调区域靶向

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-31 Epub Date: 2026-04-28 DOI: 10.1002/anbr.202500300

Alexander Chen, Michael B. Nguyen, Elliot Widd, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Houston Harding, Chloe Hurteau, Anika Yogi, Colin Luo, Rebecca L. Braden, Pedro Cabrales, Karen L. Christman

{"title":"Tunable Regional Targeting of Self-Assembling Peptide Nanomaterials in Acute Myocardial Infarction","authors":"Alexander Chen, Michael B. Nguyen, Elliot Widd, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Houston Harding, Chloe Hurteau, Anika Yogi, Colin Luo, Rebecca L. Braden, Pedro Cabrales, Karen L. Christman","doi":"10.1002/anbr.202500300","DOIUrl":"https://doi.org/10.1002/anbr.202500300","url":null,"abstract":"Acute myocardial infarction (AMI) causes cardiac tissue damage and creates a transient leaky vasculature, which is an attractive target for intravascular therapeutics. Nanomaterials have the potential to deliver therapeutics to the injured heart, yet the influence of particle size, shape, and surface decoration on infarct localization has not been systematically examined. Here, we evaluated a self-assembling peptide platform that can be tuned to form high aspect ratio nanofibers or globular nanoparticles by modifying the β sheet forming domain. This platform has tunable nanofiber length distribution via sonication and aging as well as surface decoration via targeting peptides. In a rat model of AMI, the incorporation of a type IV collagen targeting peptides significantly increased localization of the nanofibers to the injured leaky vasculature while globular peptides diffuse through and targeted the infarcted myocardium. These results demonstrate that this modular peptide nanomaterial can be tuned via geometry and surface moieties to either target to the compromised vasculature components or infiltrate the infarct. This study establishes size, shape, and surface decoration as critical features for effective intravascular nanomaterial delivery after AMI.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500300","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148859965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanodiamonds as Bioactive Platforms to Modulate Microbial, Mammalian, and Vertebrate Systems 纳米金刚石作为调节微生物、哺乳动物和脊椎动物系统的生物活性平台

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-31 Epub Date: 2026-05-28 DOI: 10.1002/anbr.202500293

Aaqil Rifai, Ayushi Priyam, Ahmed Rashed, Gebreselassie Addisu Tilaye, Kamal Poudel, Aaron G. Schultz, Richard J. Williams

{"title":"Nanodiamonds as Bioactive Platforms to Modulate Microbial, Mammalian, and Vertebrate Systems","authors":"Aaqil Rifai, Ayushi Priyam, Ahmed Rashed, Gebreselassie Addisu Tilaye, Kamal Poudel, Aaron G. Schultz, Richard J. Williams","doi":"10.1002/anbr.202500293","DOIUrl":"https://doi.org/10.1002/anbr.202500293","url":null,"abstract":"Nanodiamonds (NDs) have garnered significant attention due to their unique potential, including displaying excellent bacterial resistance and favourable interactions with mammalian cells. Here, we show multifaceted biocompatibility of NDs both in vitro and in vivo. The NDs show enhanced mammalian biocompatibility via the key markers of proliferation and differentiation of mouse bone stromal (Kusa4b10), human kidney (HEK293), and mouse dermal fibroblast (L929) cells. Importantly, high surface area, hydrophilicity, and chemical stability contribute to their antibacterial efficacy against various bacterial strains. Our findings reveal that Escherichia coli is more sensitive to NDs than Staphylococcus aureus although both E. coli and S. aureus show a significantly reduced viability of 8% with exposure to 10 mg/mL of ND. To further confirm their potential in vivo, zebrafish embryos exposed to NDs were observed to display normal development and healthy spine formation, indicating the absence of teratogenic effects. These results position NDs as promising candidates for biomedical applications, including drug delivery, biosensing, and tissue engineering.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500293","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148859982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposomal Delivery of L-2-Hydroxyglutarate for Targeting Epigenetic Dysregulation in Osteoarthritis 脂质体递送l -2-羟戊二酸靶向骨关节炎表观遗传失调

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-31 Epub Date: 2026-04-24 DOI: 10.1002/anbr.202500250

Denise Murgia, Akshay Pandey, Raffaele Spanò, Bianca Martins Estevão, Roberto Palomba, Greta Avancini, Tamaghna Gupta, Ana Maria Lopez Estevez, Stuart B. Goodman, Nidhi Bhutani, Paolo Decuzzi

{"title":"Liposomal Delivery of L-2-Hydroxyglutarate for Targeting Epigenetic Dysregulation in Osteoarthritis","authors":"Denise Murgia, Akshay Pandey, Raffaele Spanò, Bianca Martins Estevão, Roberto Palomba, Greta Avancini, Tamaghna Gupta, Ana Maria Lopez Estevez, Stuart B. Goodman, Nidhi Bhutani, Paolo Decuzzi","doi":"10.1002/anbr.202500250","DOIUrl":"https://doi.org/10.1002/anbr.202500250","url":null,"abstract":"Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degradation, inflammation, and dysregulated chondrocyte metabolism. Increasing evidence suggests that epigenetic modifications, particularly alterations in DNA hydroxymethylation regulated by ten-eleven translocation (TET) enzymes, contribute to OA pathogenesis. Notably, the TET1 inhibitor L-2-hydroxyglutarate (L-2HG) has been reported to mitigate OA-related changes by modulating 5-hydroxymethylcytosine (5hmC) levels. However, its efficacy is impaired by rapid intra-articular clearance, requiring frequent high-dose administration of the free drug. To address this, we developed a liposomal formulation of L-2HG (2HG-LIP) via microfluidic techniques and characterized it in vitro and in preclinical models of OA upon intra-articular administration. 2HG-LIP demonstrated favorable physicochemical properties, including homogeneous size (∼150 nm), low polydispersity (∼0.14), high encapsulation efficiency (∼51%), and a suitable release profile. In primary human chondrocytes from healthy and OA donors, 2HG-LIP exhibited excellent biocompatibility, efficient cellular uptake, and significant downregulation of inflammatory and catabolic genes, such as IL-1β, IL-6, MMP-3, and MMP-13, upon IL-1β pro-inflammatory stimulation. In vivo, a single intra-articular injection of 2HG-LIP significantly reduced cartilage damage, MMP-13 expression, and 5hmC accumulation. Collectively, these findings support 2HG-LIP as a promising locally delivered epigenetic therapy for OA, with potential for further evaluation in post-traumatic OA models.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500250","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic Nanoparticle Delivering Colchicine to Target Endothelial Inflammation for Atherosclerosis Treatment 仿生纳米颗粒递送秋水仙碱治疗动脉粥样硬化内皮炎症

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-31 Epub Date: 2026-05-23 DOI: 10.1002/anbr.202600004

Xiuwen Zhang, Lingqing Zong, Ashish Misra, Sanjay Patel, Zi Gu

{"title":"Biomimetic Nanoparticle Delivering Colchicine to Target Endothelial Inflammation for Atherosclerosis Treatment","authors":"Xiuwen Zhang, Lingqing Zong, Ashish Misra, Sanjay Patel, Zi Gu","doi":"10.1002/anbr.202600004","DOIUrl":"https://doi.org/10.1002/anbr.202600004","url":null,"abstract":"Dysfunctional endothelium with chronic inflammation represents an early stage in the development of atherosclerotic plaques. Nanomedicines capable of targeting activated endothelium and resolving inflammation have great potential for atherosclerosis treatment. In this work, we developed biomimetic platelet membrane-coated layered double hydroxide nanoparticles (PM-LDH) for the targeted delivery of anti-inflammatory drug colchicine to the inflamed endothelial cells and showed improved therapeutic effects by using the colchicine-loaded PM-LDH. Inspired by the natural interactions between platelets and activated endothelium, platelet membrane-coated nanoparticles exhibited markedly high affinity for inflamed endothelial cells while reducing immune clearance by macrophages. The enhanced anti-inflammatory efficacy was demonstrated by reduced monocyte recruitment and attenuated reactive oxygen species production in inflamed endothelial cells. Overall, through the platelet membrane–endothelium interaction, the biomimetic colchicine-loaded nanoparticles show strong potential as a targeted therapeutic strategy for atherosclerosis.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202600004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Receptor-Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex 受体介导的胞吞作用及其在TFAMoplex增强DNA转染中的应用比较

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-02 Epub Date: 2026-04-14 DOI: 10.1002/anbr.70124

David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean-Christophe Leroux

{"title":"Comparison of Receptor-Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex","authors":"David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean-Christophe Leroux","doi":"10.1002/anbr.70124","DOIUrl":"https://doi.org/10.1002/anbr.70124","url":null,"abstract":"Receptor-targeting ligands can improve the specificity and cellular uptake of drugs and gene delivery systems. However, there is currently a lack of rapid, high-throughput assays to identify ligands that efficiently induce endocytosis. To address this gap, we developed a flow cytometric assay that selectively quantifies internalized fluorescence, directly converting endosomal uptake into an easily measurable signal across entire cell populations and avoiding the ambiguity and labor intensity of microscopy-based methods. Using this platform, we identified ligands that induce cellular uptake and applied them to the TFAMoplex, a modular, protein-based nonviral gene delivery system recently developed in our group. Coupling of heparin-binding epidermal growth factor, identified as an efficient internalizing ligand with the endocytosis assay, enhanced TFAMoplex uptake and transfection efficacy. These results support a generalizable strategy to rapidly screen uptake ligands, enabling the rational design of improved nonviral gene delivery systems.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 8","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.70124","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Leukocyte Elastase Triggered Nanoparticles for Targeted Antimicrobial Delivery and Oral Microbial Modulation 人类白细胞弹性酶触发纳米颗粒靶向抗微生物递送和口服微生物调节

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-02 Epub Date: 2026-04-23 DOI: 10.1002/anbr.202500303

Mohammed A. Hadis, Ignacio Insua, Farah N. S. Raja, Álvaro Vila, William M. Palin, Michael R. Milward, Paul R. Cooper, Sarah A. Kuehne, Owen Addison, Paco Fernandez-Trillo

{"title":"Human Leukocyte Elastase Triggered Nanoparticles for Targeted Antimicrobial Delivery and Oral Microbial Modulation","authors":"Mohammed A. Hadis, Ignacio Insua, Farah N. S. Raja, Álvaro Vila, William M. Palin, Michael R. Milward, Paul R. Cooper, Sarah A. Kuehne, Owen Addison, Paco Fernandez-Trillo","doi":"10.1002/anbr.202500303","DOIUrl":"https://doi.org/10.1002/anbr.202500303","url":null,"abstract":"This study investigated targeted antimicrobial delivery through enzyme-responsive polyelectrolyte complex (PIC) nanoparticles of peptides and antimicrobials. Nanoparticles released chlorhexidine upon enzymatic activation by human leukocyte elastase (HLE), mimicking conditions present at inflamed or infected sites, and utilized as a semi-specific trigger for oral disease. Peptides P1-P4 and P6-P8 were tailored with HLE cleavable sequence alanine–alanine–proline–valine (AAPV) and flanked with cysteine and glutamic acid residues to enhance solubility, disulfide oxidation, and HLE sensitivity and specificity. Dynamic light scattering confirmed particle formation across various antimicrobial biguanide and peptide carboxylate ratios (N:COOH), with optimal size distribution and autocorrelation profiles observed for P7 (ECAAPVCE; N:COOH of 1:0.3). P7-chlorhexidine-based nanoparticles demonstrated structural stability in physiological conditions. Nanoparticle treatment of commensal Streptococcus sanguinis, and pathogenic Streptococcus mutans and Porphyromonas gingivalis delayed bacterial growth in a time-, concentration-, and HLE-dependent manner (p < 0.05). Subinhibitory effects without HLE suggested residual chlorhexidine was below the minimum inhibitory concentration, while the presence of HLE enhanced antimicrobial effects, leading to complete inhibition of pathogenic species and transient growth inhibition of commensal species. These findings support development of HLE-responsive nanoparticle systems for targeted antimicrobial delivery, with potential for modulating microbial communities and providing site-specific therapy in oral and other inflammatory infections.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 8","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonviral Gold Nanoparticle-Mediated Delivery of CRISPR-Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells 非病毒金纳米颗粒介导的CRISPR-Cas9核糖核蛋白和长DNA转基因进入原代血细胞。

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-02 Epub Date: 2026-03-31 DOI: 10.1002/anbr.202500242

Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair

{"title":"Nonviral Gold Nanoparticle-Mediated Delivery of CRISPR-Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells","authors":"Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair","doi":"10.1002/anbr.202500242","DOIUrl":"10.1002/anbr.202500242","url":null,"abstract":"Gene editing using CRISPR systems has gained traction for its potential to treat various diseases, but delivery remains a major challenge. We have previously reported an entirely synthetic gold nanoparticle formulation which can safely and effectively deliver multiple different CRISPR systems as ribonucleoprotein (RNP) into hematopoietic stem cells and immune cells. Here, we describe a modified version of this nanoparticle to simultaneously deliver CRISPR Cas9 RNP and transgene-encoding DNA templates (HDT) as long as ~2.1 kb. We evaluate this HDT-CRISPR-AuNP for gene editing at two loci of interest with different transgene cargo. These nanoparticles successfully deliver gene editing into primary human T cells and hematopoietic stem and progenitor cells with insertion of an antigen-specific T cell receptor transgene. This proof-of-concept immune engineering study demonstrates a simple, synthetic nanoformulation for co-delivery of all cargo required for CRISPR-mediated delivery of transgene cassettes with potential for efficacy in vivo.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 8","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13138722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable Bone Cements: A Generational Framework for Bioactivity, Porosity, and Mechanobiological Design at the Nanoscale 可注射骨水泥：纳米级生物活性、孔隙度和机械生物学设计的世代框架

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-02 Epub Date: 2026-04-15 DOI: 10.1002/anbr.202500311

Frank Fei, Yogambha Ramaswamy, Young Jung No

{"title":"Injectable Bone Cements: A Generational Framework for Bioactivity, Porosity, and Mechanobiological Design at the Nanoscale","authors":"Frank Fei, Yogambha Ramaswamy, Young Jung No","doi":"10.1002/anbr.202500311","DOIUrl":"https://doi.org/10.1002/anbr.202500311","url":null,"abstract":"Injectable bone cements (IBCs) are widely used in orthopaedic and craniofacial applications due to their minimally invasive delivery and ability to provide early mechanical stabilisation. Despite substantial progress, clinical translation remains limited by persistent challenges, including insufficient bioactivity, poor control of porosity and interconnectivity, and the limited integration of mechanobiological design principles. Additional constraints arise from the need to balance injectability, setting behaviour, and mechanical performance. Most existing reviews categorise IBCs by material composition, often overlooking important conceptual advances in functionality. Here, we propose a generation-based classification framework that captures successive shifts in injectable cement design, from first-generation bioinert structural fillers to second-generation resorbable, osteoconductive systems, to third-generation bioactivity-enhanced doped and composite formulations, and finally to fourth-generation cements incorporating hierarchical macro- and nanoscale architectures. This latest generation integrates nanomaterials and engineered pore structures to regulate cell–matrix interactions, mechanotransduction, and tissue regeneration. Across each generation, we critically examine the interdependence of biological, mechanical, and rheological properties, highlighting how architectural and mechanical cues modulate cellular responses. By synthesising advances in materials science and mechanobiology, this review outlines emerging strategies for the design of regenerative, mechanobiologically informed IBCs.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 8","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0