Advanced Nanobiomed Research最新文献_第2页

Nanoplasmonics for Enhanced Fluorescence Detection of Nucleic Acids: From Fundamentals to Boosting Cancer Management

IF 4

Advanced Nanobiomed Research Pub Date : 2025-01-23 DOI: 10.1002/anbr.202400088

David Botequim, Rui Oliveira-Silva, Vanda Vaz Serra, Peter Zijlstra, Duarte Miguel de França Prazeres, Pedro Miguel Ribeiro Paulo

{"title":"Nanoplasmonics for Enhanced Fluorescence Detection of Nucleic Acids: From Fundamentals to Boosting Cancer Management","authors":"David Botequim, Rui Oliveira-Silva, Vanda Vaz Serra, Peter Zijlstra, Duarte Miguel de França Prazeres, Pedro Miguel Ribeiro Paulo","doi":"10.1002/anbr.202400088","DOIUrl":"https://doi.org/10.1002/anbr.202400088","url":null,"abstract":"Cancer remains a leading cause of mortality and morbidity worldwide. Consequently, the scientific community continues to pursue improvements in diagnostic methods and subsequent treatments. To enhance treatment efficacy and reduce the probability of adverse outcomes, reliable and sensitive diagnostic methods are essential. A potential solution may lie in the synergy between nanotechnology and optical biosensors, as they can provide exceptional sensitivity in the detection of disease biomarkers. This review focuses on fluorescent DNA probes assembled onto metal nanoparticles for cancer-related applications. These hybrid biosensors exhibit remarkable and versatile optical properties enabling to enhance signal emission by orders of magnitude. In these configurations, metallic particles function as optical antennas for fluorescent dyes, significantly increasing their photon emission rates. This review presents a novel perspective on recent advancements in cancer diagnostics and treatment utilizing hybrid biosensors. Current methodologies for cancer diagnostics are examined, toward elucidating their advantages and limitations. The subject matter is critically evaluated, and fundamental concepts are explored to assess the most promising avenues for clinical applications. These biosensors may potentially integrate into medical practice and healthcare in the near future, both for the diagnosis and prognosis of cancer, as well as for precision (P4) medicine.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrodynamic Cavitation-Induced Thrombolysis on a Clot-on-a-Chip Platform

IF 4

Advanced Nanobiomed Research Pub Date : 2025-01-07 DOI: 10.1002/anbr.202570011

Beyzanur Ozogul, Unal Akar, Rabia Mercimek, Farzad Rokhsar Talabazar, Seyedali Seyedmirzaei Sarraf, Araz Sheibani Aghdam, Ali Ansari Hamedani, Luis Guillermo Villanueva, Dmitry Grishenkov, Ehsan Amani, Tugrul Elverdi, Morteza Ghorbani, Ali Koşar

引用次数: 0

A State-of-the-Art Appraisal of Nanomaterials in Microneedles in the Delivery of Therapeutics and Vaccines

IF 4

Advanced Nanobiomed Research Pub Date : 2024-12-26 DOI: 10.1002/anbr.202400162

Bwalya Angel Witika, Aaron Hutton, Pedzisai A. Makoni, Ryan F. Donnelly

引用次数: 0

Detection of Estrogen Receptor Status in Breast Cancer Cytology Samples by an Optical Nanosensor

IF 4

Advanced Nanobiomed Research Pub Date : 2024-12-13 DOI: 10.1002/anbr.202400099

Pooja V. Gaikwad, Nazifa Rahman, Pratyusha Ghosh, Dianna L. Ng, Ryan M. Williams

{"title":"Detection of Estrogen Receptor Status in Breast Cancer Cytology Samples by an Optical Nanosensor","authors":"Pooja V. Gaikwad, Nazifa Rahman, Pratyusha Ghosh, Dianna L. Ng, Ryan M. Williams","doi":"10.1002/anbr.202400099","DOIUrl":"https://doi.org/10.1002/anbr.202400099","url":null,"abstract":"Breast cancer is a substantial source of morbidity and mortality worldwide. Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) are the primary biomarkers which inform breast cancer treatment. Although endocrine therapy for ER+ patients is widely available, there is a need for increased access to low-cost, rapid, and accurate ER testing methods. In this work, we designed a near-infrared optical nanosensor using single-walled carbon nanotubes (SWCNT) as the transducer and an anti-ERα antibody as the recognition element. We evaluated the nanosensor in vitro prior to testing with 26 breast cancer samples which were collected by scraping the cut surface of fresh, surgically resected tumors. Twenty samples were ER+, and six ER−, representing 13 unique patients. We found that the nanosensor can differentiate ER− from ER+ patient biopsies through a shift in its center wavelength upon sample addition. Receiver operating characteristic area under the curve analyses determined that the strongest classifier with an AUC of 0.94 was the (7,5) SWCNT after direct incubation and measurement, and without further processing. We anticipate that further testing and development of this nanosensor may push its utility toward field-deployable, rapid ER subtyping with the potential for additional molecular marker profiling.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400099","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143114665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soft Cardiac Patch Using a Bifacial Architecture of Adhesive/Low-Impedance Hydrogel Nanocomposites and Highly Conductive Elastomer Nanocomposites

IF 4

Advanced Nanobiomed Research Pub Date : 2024-12-13 DOI: 10.1002/anbr.202400143

Jeeyoung Kim, Gi Doo Cha, Minsung Kim, Seung-Pyo Lee, Sung-Hyuk Sunwoo, Dae-Hyeong Kim

{"title":"Soft Cardiac Patch Using a Bifacial Architecture of Adhesive/Low-Impedance Hydrogel Nanocomposites and Highly Conductive Elastomer Nanocomposites","authors":"Jeeyoung Kim, Gi Doo Cha, Minsung Kim, Seung-Pyo Lee, Sung-Hyuk Sunwoo, Dae-Hyeong Kim","doi":"10.1002/anbr.202400143","DOIUrl":"https://doi.org/10.1002/anbr.202400143","url":null,"abstract":"\u0000Soft implantable multichannel cardiac electrode arrays that establish direct monolithic interfaces with the heart are key components for advanced cardiac monitoring and electrical modulation. A significant technological advancement in this area is the development of stretchable conductive nanocomposites, fabricated through the integration of metallic nanomaterials and elastic polymers, aimed at achieving both high electrical conductivity and mechanical elasticity. Despite these advances, further progress in material performance and device designs is required to ensure seamless, reliable, biocompatible, and high-fidelity cardiac interfacing. Herein, the development of a soft multichannel cardiac patch based on a bifacial architecture of adhesive/low-impedance hydrogel nanocomposites and highly conductive elastomer nanocomposites is reported. The bifacial design facilitates the integration of the cardiac patch between the heart and other tissues/organs can be achieved. The hydrogel nanocomposite layer, positioned on the epicardial side, provides stable adhesion to the target cardiac tissue and enables low-impedance biocompatible interfacing with the heart, while the elastomer nanocomposite layer, positioned on the opposite side, offers high electrical conductivity for facile electrophysiological signal transfer and a low-friction surface minimizing unwanted interactions with surrounding tissues. The effectiveness of this bifacial patch in multiple applications involving various cardiac signal recordings and electromechanical modulation demonstrations is showcased.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic Encapsulation of DNAs in Liquid Beads for Digital Loop-Mediated Isothermal Amplification 用于数字环介导等温扩增的液体珠中dna的微流控封装

IF 4

Advanced Nanobiomed Research Pub Date : 2024-12-11 DOI: 10.1002/anbr.2024700121

Fariba Malekpour Galogahi, Simon Strachan, Ajeet Singh Yadav, Helen Stratton, Nam-Trung Nguyen

引用次数: 0

Antibacterial Activity and Mechanisms of Magnesium-Doped Baghdadite Bioceramics for Orthopedic Implants

IF 4

Advanced Nanobiomed Research Pub Date : 2024-11-29 DOI: 10.1002/anbr.202400119

Huu Ngoc Nguyen, Iman Roohani, Andrew Hayles, Zufu Lu, Jitraporn Vongsvivut, Krasimir Vasilev, Vi Khanh Truong, Hala Zreiqat

{"title":"Antibacterial Activity and Mechanisms of Magnesium-Doped Baghdadite Bioceramics for Orthopedic Implants","authors":"Huu Ngoc Nguyen, Iman Roohani, Andrew Hayles, Zufu Lu, Jitraporn Vongsvivut, Krasimir Vasilev, Vi Khanh Truong, Hala Zreiqat","doi":"10.1002/anbr.202400119","DOIUrl":"https://doi.org/10.1002/anbr.202400119","url":null,"abstract":"Baghdadite (BAG, Ca3ZrSi2O9), a calcium silicate compound with zirconium incorporation, shows significant potential in medical implants. However, its susceptibility to infections poses a considerable challenge. To tackle this problem, doping biocompatible magnesium (Mg) into BAG to create Mg-BAG enhances antibacterial activity and prevents infection in orthopedic implants. Mg-BAG demonstrates effectiveness against Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. This study finds that the antibacterial activity of Mg-BAG is multifaced including causing the generation of reactive oxygen species (ROS) within cells and disrupting membrane potential, resulting in leakage of intracellular contents. The synchrotron macro attenuated total reflectance Fourier-transform infrared microspectroscopy shows the impact of Mg-BAG on bacteria, resulting in modifications to biomolecules such as lipids, protein structures, and the stability of nucleic acids. The combined effect of Mg ions (Mg2+) and intracellular ROS formation contributes to the disruption of biomolecules and bacterial cell death. Mg-BAG is a promising next-generation bioceramic offering innovative nonantibiotic solutions for preventing infection.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cGAMP-Containing Hydrogel for Prolonged SARS-CoV-2 Receptor-Binding Domain Subunit Vaccine Exposure Induces a Broad and Potent Humoral Response

IF 4

Advanced Nanobiomed Research Pub Date : 2024-11-28 DOI: 10.1002/anbr.202400077

Volker Böhnert, Emily C. Gale, Lauren J. Lahey, Jerry Yan, Abigail E. Powell, Ben S. Ou, Jacqueline A. Carozza, Lingyin Li, Eric A. Appel

{"title":"A cGAMP-Containing Hydrogel for Prolonged SARS-CoV-2 Receptor-Binding Domain Subunit Vaccine Exposure Induces a Broad and Potent Humoral Response","authors":"Volker Böhnert, Emily C. Gale, Lauren J. Lahey, Jerry Yan, Abigail E. Powell, Ben S. Ou, Jacqueline A. Carozza, Lingyin Li, Eric A. Appel","doi":"10.1002/anbr.202400077","DOIUrl":"https://doi.org/10.1002/anbr.202400077","url":null,"abstract":"\u0000The receptor-binding domain (RBD) of the SARS-CoV-2 virus spike protein has emerged as a promising target for the generation of neutralizing antibodies. Although the RBD subunit is more stable than its encoding mRNA, RBD is poorly immunogenic. It is hypothesized that this limitation can be overcome by sustained coadministration with a more potent and optimized adjuvant than standard adjuvants. One such candidate adjuvant, cGAMP, exhibits promising potency via activation of the antiviral STING pathway. Unfortunately, delivery of cGAMP as a therapeutic exhibits poor performance due to poor pharmacokinetics and pharmacodynamics from rapid excretion and degradation. To overcome these limitations, it is sought to create an artificial immunological niche enabling the slow release of cGAMP and RBD to mimic natural infections in which immune-activating molecules are colocalized with antigen. Specifically, through coencapsulation of cGAMP and RBD in an injectable polymer-nanoparticle (PNP) hydrogel, the cGAMP-adjuvanted hydrogel vaccine elicits more potent, durable, and broad antibody responses with improved neutralization as compared to dose-matched bolus controls and hydrogel-based vaccines lacking cGAMP. The cGAMP-adjuvanted hydrogel platform can be further explored for the delivery of other antigens to enhance immunity against a broad range of pathogens.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400077","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradable Oxygen-Generating Microneedle Patches for Regenerative Medicine Applications

IF 4

Advanced Nanobiomed Research Pub Date : 2024-11-27 DOI: 10.1002/anbr.202400093

Lindsay Barnum, Mohamadmahdi Samandari, Yasir Suhail, Steven Toro, Ashkan Novin, Pejman Ghelich, Jacob Quint, Farnooosh Saeedinejad, Manu Komma, Kshitiz, Ali Tamayol

{"title":"Biodegradable Oxygen-Generating Microneedle Patches for Regenerative Medicine Applications","authors":"Lindsay Barnum, Mohamadmahdi Samandari, Yasir Suhail, Steven Toro, Ashkan Novin, Pejman Ghelich, Jacob Quint, Farnooosh Saeedinejad, Manu Komma,  Kshitiz, Ali Tamayol","doi":"10.1002/anbr.202400093","DOIUrl":"https://doi.org/10.1002/anbr.202400093","url":null,"abstract":"Upon injury, regenerating skin is metabolically active and requires oxygen for physiological processes related to wound healing. Such processes can be halted in hypoxic conditions common in chronic wounds. Microneedle arrays (MNAs) have been demonstrated to improve therapeutic delivery and wound healing. Recently, few studies have explored the use of oxygen-releasing MNAs; however, they involve complex manufacturing and handling and fail to eliminate cytotoxic byproducts. To address these challenges, biodegradable and mechanically robust gelatin methacryloyl-based MNAs are developed that can penetrate the tissue and release oxygen upon exposure to interstitial fluid and wound exudates. The oxygen release rate and biocompatibility of the developed MNAs with different compositions are evaluated and optimized. Interestingly, in vitro studies demonstrate that the optimized compositions can release oxygen at therapeutic levels and significantly increase viability of chronically hypoxic cells to match that of normoxic cells. In vivo studies further confirm that the optimized oxygen-generating MNAs do not cause any harm or impair healing in a murine model of acute skin injury. Additionally, transcriptomic analysis reveals upregulation of key pathways related to fibroblast motility, lipid metabolism, and a marked reduction in inflammatory signaling, all of which contribute to improved wound healing. The developed strategy can introduce new opportunities in elimination of hypoxia and therefore treatment of chronic wounds.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MXene Nanoparticles: Orchestrating Spherioidogenesis for Targeted Osteogenic and Neurogenic Differentiation

IF 4

Advanced Nanobiomed Research Pub Date : 2024-11-23 DOI: 10.1002/anbr.202400100

Yoonjoo Kang, Hyeongtaek Park, Surim Shim, Gul Karima, Subeen Lee, Kisuk Yang, Hwan D. Kim

{"title":"MXene Nanoparticles: Orchestrating Spherioidogenesis for Targeted Osteogenic and Neurogenic Differentiation","authors":"Yoonjoo Kang, Hyeongtaek Park, Surim Shim, Gul Karima, Subeen Lee, Kisuk Yang, Hwan D. Kim","doi":"10.1002/anbr.202400100","DOIUrl":"https://doi.org/10.1002/anbr.202400100","url":null,"abstract":"\u0000MXenes represent a new class of 2D materials and exhibit unique properties that render them promising candidates for biomedical applications. MXenes can interact with cell membranes and modulate cell junction interactions, thereby influencing stem cell fate. While previous studies have demonstrated their potential to induce cell differentiation, research on their effects on stem cell spheroid growth and differentiation capacity is limited. This study investigates the ability of MXenes to induce cell differentiation using spheroids, which mimic the in vivo 3D microenvironment and hold significance for bone and nerve regeneration. MXene-induced spheroids of human adipose-derived mesenchymal stem cells (hADSCs) and human neural stem cells (hNSCs) rapidly aggregate, indicating MXene's role in spheroid formation. The differentiation of these spheroids confirms MXene's ability to induce specific cell types: hADSC spheroids show enhanced osteogenic differentiation at a 5 μg mL−1 concentration, while hNSC spheroids require higher concentrations (20 μg mL−1) for neuronal differentiation, possibly due to MXene's influence on intercellular adhesion. These findings highlight the potential of MXene particles in promoting rapid aggregation and differentiation of hADSC and hNSC spheroids, offering promise for applications in tissue engineering, specifically in bone and nerve regeneration.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 3","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0