Advanced Nanobiomed Research最新文献_第3页

Time-of-Flight Secondary Ion Mass Spectrometry-Partial Least Square Regression for Quantifying Interleukin-8 in Biopolymer Matrices 飞行时间二次离子质谱-偏最小二乘回归定量生物聚合物基质中白细胞介素-8

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-06-02 DOI: 10.1002/anbr.202500066

Ralf Zimmermann, Mirko Nitschke, Marten Samulowitz, Nicholas R. Dennison, Carsten Werner

{"title":"Time-of-Flight Secondary Ion Mass Spectrometry-Partial Least Square Regression for Quantifying Interleukin-8 in Biopolymer Matrices","authors":"Ralf Zimmermann, Mirko Nitschke, Marten Samulowitz, Nicholas R. Dennison, Carsten Werner","doi":"10.1002/anbr.202500066","DOIUrl":"10.1002/anbr.202500066","url":null,"abstract":"Unraveling the complexity of biomatrices is a persisting challenge in many areas of the life sciences. The detection of soluble signaling molecules—cytokines and growth factors—within multicomponent biopolymer scaffolds is of particular interest as they control important biological processes such as the development of tissues, pathologies, and regeneration. The application of time-of-flight secondary ion mass spectrometry (ToF-SIMS) for the detection of interleukin-8 (IL-8), a chemokine involved in inflammation and cancer, is explored within biopolymer matrices of different complexity. To establish the workflow, IL-8 is embedded with graded mass fractions in thin biopolymer matrices consisting of heparin and/or bovine serum albumin, followed by a comprehensive ToF-SIMS analysis of the prepared samples. Partial least square regression models are developed and successfully applied to detect IL-8 mass fractions down to 1 ppm on the basis of the measured ToF-SIMS spectra. The methodology is successfully applied to detect IL-8 in Matrigel and poly(ethylene glycol)-heparin matrices with similar sensitivity. Given the high performance of state-of-the-art SIMS instruments and the increasing power of machine learning algorithms, it is envisioned that the established approach, in combination with other methods, will enable a comprehensive assessment of soluble signaling molecules in (engineered) matrix-supported 3D cell and organoid cultures.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprinting-Assisted Tissue Assembly to Investigate Endothelial Cell Contributions in Cardiac Fibrosis and Focal Fibrosis Modeling 生物打印辅助组织组装研究内皮细胞在心脏纤维化和局灶性纤维化模型中的作用

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-06-02 DOI: 10.1002/anbr.202400148

Dong Gyu Hwang, Hwanyong Choi, Myungji Kim, Minji Kim, Donghwan Kim, Jinseon Park, Jinah Jang

{"title":"Bioprinting-Assisted Tissue Assembly to Investigate Endothelial Cell Contributions in Cardiac Fibrosis and Focal Fibrosis Modeling","authors":"Dong Gyu Hwang, Hwanyong Choi, Myungji Kim, Minji Kim, Donghwan Kim, Jinseon Park, Jinah Jang","doi":"10.1002/anbr.202400148","DOIUrl":"https://doi.org/10.1002/anbr.202400148","url":null,"abstract":"Cardiac fibrosis is characterized by excessive extracellular matrix (ECM) deposition, driven by the activation of cardiac fibroblasts (cFBs) and endothelial-to-mesenchymal transition (EndMT). Endothelial cells (ECs) contribute to cardiac fibrosis through EndMT, transforming into myofibroblasts that promote fibrosis, while also playing a regulatory role through signaling pathways, such as PI3K-Akt and Notch. In this article, engineered heart tissue models, composed of cardiomyocytes and cFBs (CMF) and vascularized model incorporating ECs (CMFE) tissues is created to investigate the role of ECs in transforming growth factor-β (TGF-β)-induced cardiac fibrosis. Prior to fibrosis induction, CMFE exhibits enhanced activation of fibrosis-related signaling, endothelial integrity pathways, and PI3K-Akt and Notch signaling compared to CMF. Following TGF-β treatment, CMF exhibits typical fibrotic features, including increased ECM deposition, tissue stiffening, and reduced contractility. In contrast, the CMFE demonstrates attenuated fibrotic responses, maintaining tissue mechanics and contractile function. Gene expression and histology reveals both fibrotic and protective processes in CMFE. Moreover, the bioprinting-assisted tissue assembly (BATA) approach enable focal fibrosis modeling, revealing that fibrotic regions disrupted calcium propagation and induced electrophysiological abnormalities. These findings highlight BATA as a promising platform for studying cardiac fibrosis and developing targeted therapeutic strategies.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 10","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145316685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances on Albumin-Based Carriers for Anticancer Drug Delivery 基于白蛋白的抗癌药物载体研究进展

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-06-01 DOI: 10.1002/anbr.202500011

Ruoli Zhou, Rongbin Zhong, Zhonghui Luo, Hua Wei, Cui-Yun Yu

引用次数: 0

Laser-Patterned Diamond Electrodes for Adhesion and Proliferation of Human Mesenchymal Stem Cells 激光图纹金刚石电极用于人间充质干细胞的粘附和增殖

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-27 DOI: 10.1002/anbr.202500041

Hassan N. Al Hashem, Kaiwen Zhang, Amanda N. Abraham, Deepak Sharma, Andre Chambers, Mehrnoosh Moghaddar, Chayla L. Reeves, Sanjay K. Srivastava, Amy Gelmi, Arman Ahnood

{"title":"Laser-Patterned Diamond Electrodes for Adhesion and Proliferation of Human Mesenchymal Stem Cells","authors":"Hassan N. Al Hashem, Kaiwen Zhang, Amanda N. Abraham, Deepak Sharma, Andre Chambers, Mehrnoosh Moghaddar, Chayla L. Reeves, Sanjay K. Srivastava, Amy Gelmi, Arman Ahnood","doi":"10.1002/anbr.202500041","DOIUrl":"10.1002/anbr.202500041","url":null,"abstract":"The ability to form diamond electrodes on insulating polycrystalline diamond substrates using single-step laser patterning and the use of these electrodes as a substrate that supports the adhesion and proliferation of human mesenchymal stem cells (hMSCs) are demonstrated. Laser-induced graphitization results in a conductive amorphous carbon surface, rich in oxygen- and nitrogen-terminated groups. This leads to an electrode with a high specific capacitance of 182 μF cm2, a wide water window of 3.25 V, and a low electrochemical impedance of 129 Ω cm2 at 1 kHz—essential attributes for effective bioelectronic cell interfaces. The electrode's surface exhibits no cytotoxic responses with hMSCs, supporting cell adhesion and proliferation. Cells cultured on the electrode display significant elongation and alignment along the direction of the laser-patterned microgrooves—an additional modality for cellular modulation. The combination of favorable electrochemical performance and effective cellular control makes laser-patterned diamond electrodes a versatile platform in stem cell therapeutics. This direct fabrication approach paves the way for the integration of diamond electrodes in bioelectronic devices, offering new opportunities for tissue engineering and electroactive biomaterial applications.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Bioreactors: A Regenerative Approach to Skeletal Muscle Engineering for Repair and Replacement” 对“生物反应器：骨骼肌修复和替代工程的再生方法”的更正

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-27 DOI: 10.1002/anbr.202500111

引用次数: 0

Extracellular Vesicles in Calcium Oxalate Nephrolithiasis: Emerging Biomarkers and Therapeutic Potential 草酸钙肾结石的细胞外囊泡：新兴的生物标志物和治疗潜力

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-20 DOI: 10.1002/anbr.202500032

Wang Zhu, Xu Changzhi, Deng Qiong, Liang Hui

{"title":"Extracellular Vesicles in Calcium Oxalate Nephrolithiasis: Emerging Biomarkers and Therapeutic Potential","authors":"Wang Zhu, Xu Changzhi, Deng Qiong, Liang Hui","doi":"10.1002/anbr.202500032","DOIUrl":"10.1002/anbr.202500032","url":null,"abstract":"Kidney stone ranks as one of the most prevalent disorders in the urology department, causing substantial personal suffering and healthcare costs globally. However, the prediction, early diagnosis, and treatment of kidney stone disease are still limited. Extracellular vesicles (EVs), loaded with nucleic acids, proteins, metabolites, and lipids, are released by a wide variety of cell types and have potential as biomarkers for kidney stone disease. Meanwhile, some natural EVs derived from plants and animals have been evidenced to have substantial effects on the elimination of calcium oxalate crystals. More importantly, recent explorations have elucidated the multifaceted role of EVs in therapeutic applications. These engineered EVs can be loaded with therapeutic RNAs, oligonucleotides, peptides, and small molecules; this approach has shown great promise in targeted drug delivery and presents a potential solution to the challenges of kidney stone prevention and treatment. This review focuses on EVs derived from blood, urine, kidney, gut microbiota, and urine bacteria, which contribute to calcium oxalate crystal elimination. The therapeutic potential of EVs is significant, offering personalized treatment options. However, it is crucial to assess the challenges in moving EV-based therapies from laboratory settings to clinical applications.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Application of a Water-Soluble Fullerene C60 Decorated with Diethylene Glycol in Treatment of Osteoarthritis 二甘醇修饰水溶性富勒烯C60在骨关节炎治疗中的潜在应用

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-20 DOI: 10.1002/anbr.202500020

Rui He, Alyssa Costello, Yanjie Liu, Weicheng Wang, Zhichang Zhang, Quanjun Cui, Xinlin Yang

{"title":"Potential Application of a Water-Soluble Fullerene C60 Decorated with Diethylene Glycol in Treatment of Osteoarthritis","authors":"Rui He, Alyssa Costello, Yanjie Liu, Weicheng Wang, Zhichang Zhang, Quanjun Cui, Xinlin Yang","doi":"10.1002/anbr.202500020","DOIUrl":"10.1002/anbr.202500020","url":null,"abstract":"Inflammatory macrophages play a role in cartilage degeneration associated with osteoarthritis (OA) via signaling cascades that result in production of inflammatory substances. This study aims to characterize compound F2, C60(NCH2CH2OCH2CH2OH)5, a newly synthesized ethoxyethanol derivative of iminofullerene, and its potential to reduce inflammatory macrophage activity. First, compound F2 is synthesized and labeled with 99mTc to create 99mTc-F2. It is then added to lipopolysaccharide (LPS)-exposed bone marrow macrophages (BMMs) to determine its effect on macrophage activation, nitric oxide production, and expression of inflammatory markers iNOS, IL-6, Fpr2, and TLR4. An animal model of osteoarthritis is also injected with 99mTc-F2 to visualize its localization in vivo. This study demonstrates successful synthesis and radiolabeling of the compound F2 molecule. It also demonstrates that compound F2 reduces nitrite production and suppresses the expression of TNF α, IL-6, iNOS, Fpr2, and TLR4 in BMMs exposed to LPS. Additionally, in rats with surgically transected anterior cruciate ligaments, intravenous administration of radioisotope-labeled compound F2 exhibits selective enrichment in the injured knee. These findings suggest that compound F2 mitigates macrophage activation, decreases inflammatory marker expression, and is located to damaged areas, highlighting its potential as a therapeutic option for OA management.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 9","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in Biomimetic Microdevices for Anticancer Drug Screening and their Potential for Enhancing In Vivo Efficacy 用于抗癌药物筛选的仿生微装置研究进展及其提高体内药效的潜力

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-14 DOI: 10.1002/anbr.202500060

Ching-Te Kuo, Yen-Tzu Liao, Hsinyu Lee

引用次数: 0

Label-Free Microscale Technologies for Isolation of Heterogeneous Circulating Tumor Cells 非标记微尺度技术分离异质循环肿瘤细胞

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-08 DOI: 10.1002/anbr.202400179

Gürhan Özkayar, Esma Derin, Georg R. Pesch, John W. M. Martens, Peter ten Dijke, Pouyan E. Boukany

{"title":"Label-Free Microscale Technologies for Isolation of Heterogeneous Circulating Tumor Cells","authors":"Gürhan Özkayar, Esma Derin, Georg R. Pesch, John W. M. Martens, Peter ten Dijke, Pouyan E. Boukany","doi":"10.1002/anbr.202400179","DOIUrl":"10.1002/anbr.202400179","url":null,"abstract":"The dissemination of primary solid tumor cells to distant organs, termed metastasis, is a major cause of cancer-related deaths. Circulating tumor cells (CTCs), which can exist as individual cells or multicellular clusters, travel through the bloodstream. Their isolation from liquid biopsy samples is increasingly recognized as a valuable tool for diagnosis, prognosis, and treatment guidance for cancer patients. Current isolation methods typically rely on biomarkers like epithelial cell adhesion molecule (EpCAM) and utilize technologies such as magnetic beads or microfluidic chips. However, these methods face limitations due to tumor heterogeneity. Furthermore, tumor cells that transfer into CTCs often undergo epithelial-to-mesenchymal transition, gaining invasive characteristics while losing epithelial markers. As a result, these cells are difficult to detect using EpCAM-based methods. Label-free microscale isolation technologies tackle the limitations of biomarker-based methods by leveraging the distinctive physical properties of CTCs, such as their size, electrical charge, viscoelasticity, and deformability that contrast them from normal blood cells. This review evaluates primary label-free isolation methods, including deterministic lateral displacement, microfiltration, acoustophoresis, and dielectrophoresis, and whether they can offer a deeper insight into tumor heterogeneity and the dynamics of cancer progression and treatment. Additionally, it highlights automated platforms for high-throughput CTC isolation and analysis.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene Oxide-Based Gene Modulation in Preferential Elimination of Lung Cancer Cells in a 3D Tumor Microenvironment Model 基于氧化石墨烯的基因调控在三维肿瘤微环境模型中优先消除肺癌细胞

IF 4.4

Advanced Nanobiomed Research Pub Date : 2025-05-07 DOI: 10.1002/anbr.202500028

Francesca Grilli, Sadman Sakib, Fabio Variola, Shan Zou

{"title":"Graphene Oxide-Based Gene Modulation in Preferential Elimination of Lung Cancer Cells in a 3D Tumor Microenvironment Model","authors":"Francesca Grilli, Sadman Sakib, Fabio Variola, Shan Zou","doi":"10.1002/anbr.202500028","DOIUrl":"10.1002/anbr.202500028","url":null,"abstract":"Lung cancer remains the leading cause of cancer-related mortality worldwide, owing to its aggressive nature, late-stage diagnosis, and resistance to conventional therapies. Gene therapy offers a promising alternative by modulating specific genetic pathways to target cancer cells while sparing healthy ones. This study investigates the potential of chemically functionalized nanoscale graphene oxide (GO) as carriers for delivering therapeutic genes in a 3D tumor microenvironment (TME) model, incorporating lung cancer cells, human lung fibroblasts, and macrophages in a Matrigel-collagen matrix to mimic the structural properties and immune functions. These therapeutic genes, including small interfering RNAs and plasmid DNAs, regulate immune evasion markers (CD47 and CD24) and apoptosis-inducing proteins (ANT1). GO nanocarriers demonstrate preferential uptake in cancer cells, achieving transfection and gene modulation within the TME model. The individual delivery of genes downregulates cancer markers and induces ANT1 expression, resulting in lung cancer cell elimination. Co-delivery of CD47_siRNA and ANT1_pDNA produces synergistic efficacy, enhancing cancer cell elimination. These findings highlight the potential of GO-based gene therapies as a targeted and effective approach for lung cancer treatment, setting the stage for in vivo validation and clinical translation.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 7","pages":""},"PeriodicalIF":4.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0