Advanced Nanobiomed Research最新文献_第3页

Therapeutic Applications of Stimuli-Based Release and Engineering of Extracellular Vesicles 基于刺激的细胞外囊泡释放和工程的治疗应用

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-02 Epub Date: 2026-04-15 DOI: 10.1002/anbr.202500173

Gloria Kemunto, Kristen Dellinger

{"title":"Therapeutic Applications of Stimuli-Based Release and Engineering of Extracellular Vesicles","authors":"Gloria Kemunto, Kristen Dellinger","doi":"10.1002/anbr.202500173","DOIUrl":"https://doi.org/10.1002/anbr.202500173","url":null,"abstract":"Extracellular vesicles (EVs), nano- to microsized lipid bilayer membrane-bound particles, are play an important role in intercellular communication and hold promise for drug delivery systems (DDSs). Their unique morphology and customizable attributes position EVs as innovative platforms for diagnostic and therapeutic applications. As DDSs, EVs should ensure precise, sustained, and targeted delivery to improve therapeutic efficacy. Thus, stimulus-responsive strategies, using endogenous (e.g., pH, hypoxia) and exogenous triggers (e.g., light, ultrasound), are currently being explored as promising strategies to achieve controlled drug release and enhanced targeting. For example, functionalizing EVs with chemical groups can enhance responsiveness, targeting, and imaging contrast. This review examines how various stimuli can be used to affect EV release, cargo loading, cell uptake, and targeting capabilities, which are crucial for advancing nanomedicine. Characterizing the impact of stimuli on EV release from cells provides essential insights into the cellular mechanisms and signaling pathways, and designing strategies to modulate EV release, aiding in developing advanced DDS, potentially minimizing side effects and enhancing targeted and sustained payload release. In sum, the review covers studies on stimulus-responsive EVs, both intrinsic and extrinsic, dual and hybrid EVs, and their therapeutic applications, elucidating the potential of engineered EVs in nanomedicine.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 8","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tensile Stimulation in Biorelevant Culture Conditions Enhances MSC and TPSC Tenogenesis on Aligned Electrospun Scaffolds 生物相关培养条件下的拉伸刺激促进定向静电纺丝支架上MSC和TPSC的肌腱生成

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-08-02 Epub Date: 2026-03-24 DOI: 10.1002/anbr.202500218

Vera Citro, Aldo R. Boccaccini, Nicholas R. Forsyth, Tina P. Dale

{"title":"Tensile Stimulation in Biorelevant Culture Conditions Enhances MSC and TPSC Tenogenesis on Aligned Electrospun Scaffolds","authors":"Vera Citro, Aldo R. Boccaccini, Nicholas R. Forsyth, Tina P. Dale","doi":"10.1002/anbr.202500218","DOIUrl":"https://doi.org/10.1002/anbr.202500218","url":null,"abstract":"Tendon injuries affect millions each year, yet effective repair remains limited by the tissue's avascular nature and complex mechanobiology. An anisotropic electrospun scaffold functionalized with GDF-7-loaded mesoporous silica nanoparticles (MSNs) is developed to address these challenges. GDF-7 is efficiently encapsulated (99.9%) in MSNs and incorporated into aligned poly(ε-caprolactone) (PCL) fibers, achieving controlled release over 46 days following non-Fickian kinetics. MSN incorporation initially reduces scaffold stiffness and tensile strength; however, dynamic bioreactor culture of human mesenchymal stromal cell (MSCs) and porcine tendon progenitor stem cell (TPSCs) seeded scaffolds, under physiological axial strain, enhances cell alignment, proliferation, and extracellular matrix deposition. Culture under physiological oxygen (2%) conditions further promotes cell viability, proliferation, and tenogenic gene expression. Notably, the combination of topographical guidance, mechanical loading, and physoxia synergistically enhances the expression of terminal tenogenic markers, such as tenomodulin (Tnmd), in both MSCs and TPSCs, as evidenced by increased protein levels detected via immunofluorescence and elevated gene expression confirmed by RT-qPCR analysis. This integration of topographical, biochemical, and mechanical cues in a tissue engineering approach supports tendon-specific lineage commitment, offering a promising strategy for functional tendon regeneration.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 8","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148652444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysyl Oxidase Reduces Neutrophil Extravasation in Response to P. aeruginosa in an Infection-on-a-Chip Model 赖氨酸氧化酶在铜绿假单胞菌感染模型中减少中性粒细胞外渗。

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-07-09 Epub Date: 2026-03-07 DOI: 10.1002/anbr.202500180

Christopher J. Calo, Tanvi Patil, Josephine Fouts, Eric L. Ginter, Margaret Radke, Hannah K. Weppner, Justin M. Owens, Laurel E. Hind

{"title":"Lysyl Oxidase Reduces Neutrophil Extravasation in Response to P. aeruginosa in an Infection-on-a-Chip Model","authors":"Christopher J. Calo, Tanvi Patil, Josephine Fouts, Eric L. Ginter, Margaret Radke, Hannah K. Weppner, Justin M. Owens, Laurel E. Hind","doi":"10.1002/anbr.202500180","DOIUrl":"10.1002/anbr.202500180","url":null,"abstract":"Neutrophils are crucial players in the fight against infections. Unfortunately, dysregulated neutrophil function contributes to the pathogenesis of diseases, including cancer, fibrosis, and atherosclerosis, that leave those afflicted vulnerable to severe infections. Many of these diseases are accompanied by differential lysyl oxidase (LOX) activity. The LOX enzyme crosslinks collagen and elastin, two highly expressed proteins in the extracellular matrix (ECM), altering the ECM's mechanical properties. While it is known ECM mechanical properties regulate neutrophil function, the role of LOX crosslinking of collagen on the neutrophil response is unclear. This study uses a microfluidic “infection-on-a-chip”. This device consists of a model blood vessel endothelium embedded in an ECM mimic to investigate how LOX crosslinking of collagen affects neutrophil function in response to infection. Interestingly, LOX-crosslinking of collagen decreases neutrophil extravasation through an endothelium in response to Pseudomonas aeruginosa compared to uncrosslinked collagen hydrogels. Critically, endothelial cells in devices with LOX-crosslinked collagen exhibited increased VE-cadherin expression compared to those seeded in uncrosslinked collagen gels, which is hypothesized to restrict neutrophil extravasation. These data demonstrate the regulatory capability of LOX over the neutrophil response, providing a potential therapeutic pathway for diseases associated with neutrophil dysregulation and LOX activity that merits further investigation.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 7","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13293553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148333495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold Gas Plasma Induces Platelet Activation and Hemostasis in Native and Anticoagulated Human Blood 低温气体血浆诱导天然血液和抗凝血的血小板活化和止血作用

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-07-09 Epub Date: 2026-03-24 DOI: 10.1002/anbr.202500305

Sander Bekeschus, Broder Poschkamp, Lea Miebach, Anke Schmidt, Linus Hübner, Julia van der Linde

{"title":"Cold Gas Plasma Induces Platelet Activation and Hemostasis in Native and Anticoagulated Human Blood","authors":"Sander Bekeschus, Broder Poschkamp, Lea Miebach, Anke Schmidt, Linus Hübner, Julia van der Linde","doi":"10.1002/anbr.202500305","DOIUrl":"https://doi.org/10.1002/anbr.202500305","url":null,"abstract":"Cold medical gas plasma promotes hemostasis through platelet activation, yet the underlying molecular and functional mechanisms remain incompletely understood. Here, we investigated gas plasma-induced platelet activation in human whole blood using flow cytometry, transcriptomic profiling, and pharmacological inhibition. Gas plasma exposure induced robust platelet activation and aggregation, accompanied by hemoglobin oxidation and adenosine diphosphate (ADP) release, but without substantial DNA liberation, consistent with selective red blood cell lysis. Transcriptomic analysis revealed significant regulation of 627 genes associated with platelet activation, inflammation, and oxidative stress responses. Pharmacological inhibition implicated Bruton's tyrosine kinase (BTK) and, to a lesser extent, Toll-like receptor 4 (TLR4) as partial mediators of gas plasma-driven activation, whereas other signaling pathways were unaffected. Hemoglobin oxidation to methemoglobin occurred linearly with treatment duration and was mediated by gas plasma-generated short-lived species. Modulating gas plasma-generated reactive species outputs could enhance its hemostatic capacity, especially when applied conductively to the treatment target. Finally, gas plasma-mediated hemostasis and platelet activation and aggregation were preserved in blood of anticoagulated patients medicated with clopidogrel or novel oral anticoagulants. Collectively, these findings demonstrate that gas plasma promotes hemostasis through redox- and receptor-dependent platelet activation, modulated by inflammasome and immune signaling pathways, and can circumvent conventional antiplatelet inhibition, supporting its translational potential as a nonthermal, tissue-preserving, pro-hemostatic modality for surgical applications.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 7","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148522787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Miniaturized Conductometric Biosensor for Rapid Detection of TNF-α in Saliva 用于唾液中TNF-α快速检测的小型电导生物传感器

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-07-09 Epub Date: 2026-02-22 DOI: 10.1002/anbr.202500277

Mingjie Yang, Ying Zhi Cheong, Chenglong Xu, Sanje Mahasivam, Xudong Cai, Vipul Bansal, Sharath Sriram, Ganganath Perera, Madhu Bhaskaran

{"title":"Miniaturized Conductometric Biosensor for Rapid Detection of TNF-α in Saliva","authors":"Mingjie Yang, Ying Zhi Cheong, Chenglong Xu, Sanje Mahasivam, Xudong Cai, Vipul Bansal, Sharath Sriram, Ganganath Perera, Madhu Bhaskaran","doi":"10.1002/anbr.202500277","DOIUrl":"https://doi.org/10.1002/anbr.202500277","url":null,"abstract":"Tumor necrosis factor-α (TNF-α) has emerged as a critical biomarker for insulin resistance and diabetic complications, offering an alternative avenue for diabetes management beyond conventional glucose monitoring. Current methods for detecting TNF-α are constrained by their complexity and lack of adaptability for home-based use. We present a novel conductometric sensor engineered for highly sensitive, selective, and rapid detection (<2 min) of TNF-α in saliva. Developed through surface modification of high-resistivity silicon wafers, the sensor achieves quantification of TNF-α in the femtomolar range with the limit of detection as low as 10 fM which is 100× times lower than that of critical concentration of TNF-α in saliva and demonstrates robust performance under varying pH conditions. The ultrahigh sensitivity for TNF-α demonstrates the capability for effective prescreening method of diabetes and the demonstration of robust linear sensor response between the clinically relevant salivary TNF-α levels illustrates its use for practical diabetes monitoring. Leveraging a Bluetooth microcontroller, the sensor integrates wireless data transmission to a smartphone, enabling rapid and user-friendly monitoring. This technology represents a significant advancement in TNF-α detection, offering a holistic approach to track disease progression and complications, with the potential to transform the early identification and monitoring of diabetic conditions.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 7","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500277","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesoporous Silica Nanoparticles With Customized Drug Ratio/Loading for Effective Treatment of Gemcitabine-Resistant Pancreatic Tumors 定制药物比/负载的介孔二氧化硅纳米颗粒有效治疗耐吉西他滨胰腺肿瘤。

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-07-09 Epub Date: 2026-03-23 DOI: 10.1002/anbr.202500255

Tamanna Binte Huq, Sudip Kumar Dam, Doaha Awad, Punnya Anil Kumar Jeeja, Farzana Ferdous, Juan L. Vivero-Escoto

{"title":"Mesoporous Silica Nanoparticles With Customized Drug Ratio/Loading for Effective Treatment of Gemcitabine-Resistant Pancreatic Tumors","authors":"Tamanna Binte Huq, Sudip Kumar Dam, Doaha Awad, Punnya Anil Kumar Jeeja, Farzana Ferdous, Juan L. Vivero-Escoto","doi":"10.1002/anbr.202500255","DOIUrl":"10.1002/anbr.202500255","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers, with a 5-year survival rate below 13% and limited treatment options due to rapid metastasis and pronounced chemoresistance. Gemcitabine (Gem) remains a first-line chemotherapy agent; however, its clinical efficacy is hindered by poor cellular uptake, incomplete activation, and acquired drug resistance. To address these limitations, we develop redox-responsive mesoporous silica nanoparticles (MSNs) for the mono- and codelivery of Gem and Cisplatin (cisPt). In this work, we tune the loading and ratio of Gem and cisPt within MSNs. To evaluate the therapeutic potential of MSN-based delivery system in Gem-resistant (GR) PDAC cell lines, we establish murine (GR-KCM) and human (GR-BxPC3) cell models and further evaluate the efficacy in highly GR human cell lines (AsPC1 and HPAFII). In vitro studies demonstrate that Gem-MSNs (10%wt) and Gem-cisPt-MSN (10:9%wt) exhibit the strongest cytotoxicity, even in GR models. Notably, the combination Gem-cisPt-MSN (18:9%wt) induces pronounced S-phase cell cycle arrest, apoptosis, and reactive oxygen species. These findings underscore the potential of MSN-based drug delivery systems to enhance chemotherapy efficacy in treatment-refractory PDAC.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 7","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147964797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium Nanomaterials for Wound Healing: Synergistic Strategies from Anti-Infection to Tissue Regeneration 硒纳米材料用于伤口愈合：从抗感染到组织再生的协同策略

IF 4.8

Advanced Nanobiomed Research Pub Date : 2026-07-09 Epub Date: 2026-03-17 DOI: 10.1002/anbr.202500206

Yangxia Chen, Xiaoshuang Liang, Sirui Li, Shuoshan Li, Wei Liu, Yingping Lin, Siqi Li, Zehang Zhang, Judun Zheng, Tianfeng Chen, Rongyi Chen

{"title":"Selenium Nanomaterials for Wound Healing: Synergistic Strategies from Anti-Infection to Tissue Regeneration","authors":"Yangxia Chen, Xiaoshuang Liang, Sirui Li, Shuoshan Li, Wei Liu, Yingping Lin, Siqi Li, Zehang Zhang, Judun Zheng, Tianfeng Chen, Rongyi Chen","doi":"10.1002/anbr.202500206","DOIUrl":"https://doi.org/10.1002/anbr.202500206","url":null,"abstract":"This review synthesises current evidence on selenium nanoparticles (SeNPs) as a therapeutic platform for wound repair. Owing to their multifunctional bioactivity, SeNPs can concurrently deliver antimicrobial, anti-inflammatory, and proregenerative effects through diverse mechanisms. They may directly disrupt bacterial cell membranes and biofilms by generating reactive oxygen species (ROS), regulate immune responses by modulating the balance between proinflammatory and anti-inflammatory cytokines, and enhance tissue repair through Nrf2-mediated antioxidant pathways that promote angiogenesis and remodelling of the extracellular matrix (ECM). Novel SeNP systems, including photothermal/photodynamic systems, have shown significant progress in improving wound healing through composite materials, immunomodulatory nanocomposites, pH/ROS-responsive hydrogels, and 3D-printed dressings. Key highlights include intelligent microenvironment-responsive designs and integrated diagnostic-therapeutic platforms. Overall, their favourable biocompatibility and notable therapeutic performance make SeNPs promising for addressing the multifactorial challenges of chronic wounds and may facilitate the clinical translation of SeNP-based interventions.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 7","pages":""},"PeriodicalIF":4.8,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500206","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148534118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogenous Model of Temozolomide Resistance in Glioblastoma Reveals Phenotypic Shifts in Drug Response and Migratory Potential 胶质母细胞瘤替莫唑胺耐药的异质性模型揭示了药物反应和迁移潜力的表型变化。

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-06-01 Epub Date: 2026-02-24 DOI: 10.1002/anbr.202500244

Victoria A. Kriuchkovskaia, Ela K. Eames, Sydney A. McKee, Paul J. Hergenrother, Rebecca B. Riggins, Brendan A. C. Harley

{"title":"Heterogenous Model of Temozolomide Resistance in Glioblastoma Reveals Phenotypic Shifts in Drug Response and Migratory Potential","authors":"Victoria A. Kriuchkovskaia, Ela K. Eames, Sydney A. McKee, Paul J. Hergenrother, Rebecca B. Riggins, Brendan A. C. Harley","doi":"10.1002/anbr.202500244","DOIUrl":"10.1002/anbr.202500244","url":null,"abstract":"Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor in adults, with limited survival outcomes due to tumor recurrence, mainly driven by GBM cell invasion and therapy resistance. Although temozolomide (TMZ) remains the standard-of-care chemotherapeutic, its long-term efficacy is often compromised by rapid emergence of acquired resistance, largely mediated by the DNA repair enzyme, methylguanine methyltransferase (MGMT). To investigate the interplay between tumor heterogeneity, drug resistance, and the extracellular matrix (ECM) microenvironment, we adapted a 3D methacrylamide-functionalized gelatin (GelMA) hydrogel model to study the behavior of mixed populations of TMZ-sensitive and TMZ-resistant GBM cells. Using both single-cell distributions and multicellular spheroids, we report the impact of heterogeneous cell populations and TMZ dosing regimens, including physiological, supraphysiological, and metronomic TMZ schedules, on drug response and migration. We show that the combination therapy of TMZ with an MGMT inhibitor, lomeguatrib, can modulate TMZ resistance in vitro. This hydrogel model enables systematic investigation of GBM heterogeneity, “go-or-grow” phenotypic plasticity, and therapeutic resistance in an ECM-rich microenvironment, offering a valuable platform for future translational research.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13038291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147595309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Molecular Diffusion Dynamics Between Subcutaneous Tissue and Microneedles for Wearable Continuous Glucose Monitoring 可穿戴式连续血糖监测中皮下组织与微针之间的分子扩散动力学研究

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-06-01 Epub Date: 2026-03-07 DOI: 10.1002/anbr.202500302

Wensheng Lin, Binbin Cui, Xuecheng He, Suo Tu, Shilei Dai, Xinran Zhang, Ivo Pang, Xiaonan Chen, Jixiang Zhu, Shiming Zhang

引用次数: 0

Beyond PEGylation: Archaeal Lipids for Long-Circulating Liposomes 超越聚乙二醇化：用于长循环脂质体的古菌脂质

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-06-01 Epub Date: 2026-02-13 DOI: 10.1002/anbr.202500232

Viktor Sedlmayr, Andreas D. Meid, Eric Mühlberg, Manuel Ben Böhmann, Julian Quehenberger, David Wurm, Mikko Gynther, Oliver Spadiut, Gert Fricker, Philipp Uhl

{"title":"Beyond PEGylation: Archaeal Lipids for Long-Circulating Liposomes","authors":"Viktor Sedlmayr, Andreas D. Meid, Eric Mühlberg, Manuel Ben Böhmann, Julian Quehenberger, David Wurm, Mikko Gynther, Oliver Spadiut, Gert Fricker, Philipp Uhl","doi":"10.1002/anbr.202500232","DOIUrl":"https://doi.org/10.1002/anbr.202500232","url":null,"abstract":"The growing concerns regarding the immunogenicity of polyethylene glycol (PEG) have prompted the search for alternative excipients that can prolong circulation in parenteral drug delivery. Archaeal lipids, known for their structural stability and unique membrane properties, represent a promising material in this context. In this study, we evaluated the impact of archaeal lipid-based liposomes on the pharmacokinetics of intravenously administered vancomycin in male Wistar rats. Liposomes containing either purified caldarchaeol (GDGT) or an archaeal lipid extract (ALE), consisting of 5 lipid species, were compared to conventional PEGylated liposomes and free vancomycin regarding their effect on systemic exposure of vancomycin. All liposomal formulations significantly enhanced exposure and yielded a prolonged circulation time compared to the free drug. GDGT containing liposomes achieved pharmacokinetic effects comparable to PEGylated systems, markedly reducing clearance and central volume of distribution based on population pharmacokinetic modeling. Our findings provide the first in vivo evidence that archaeal lipids, particularly GDGT, might act as effective PEG replacement excipient for parenteral delivery, offering a promising alternative platform.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500232","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148109685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0