Advanced Nanobiomed Research最新文献_第3页

Biomimetic Nanomaterials for Osteoarthritis Treatment: Targeting Cartilage, Subchondral Bone, and Synovium 用于骨关节炎治疗的仿生纳米材料：针对软骨、软骨下骨和滑膜

IF 4

Advanced Nanobiomed Research Pub Date : 2024-10-24 DOI: 10.1002/anbr.202400029

Xiaoshan Gong, Hao Tang, Jingjin Dai, Guoqiang Wang, Shiwu Dong

{"title":"Biomimetic Nanomaterials for Osteoarthritis Treatment: Targeting Cartilage, Subchondral Bone, and Synovium","authors":"Xiaoshan Gong, Hao Tang, Jingjin Dai, Guoqiang Wang, Shiwu Dong","doi":"10.1002/anbr.202400029","DOIUrl":"https://doi.org/10.1002/anbr.202400029","url":null,"abstract":"Osteoarthritis (OA) is characterized mainly by articular cartilage loss, subchondral osteosclerosis, and chronic inflammation and involves multiple types of cellular dysfunction and tissue lesions. The rapid development of nanotechnology and materials science has contributed to the application of biomimetic nanomaterials in the biomedical field. By optimizing the composition, hardness, porosity, and drug loading of biomimetic nanomaterials, their unique physicochemical properties drive potential applications in bone repair. This article reviews the present understanding of the physiopathological mechanism and clinical treatment drawbacks of OA and summarizes various types of biomimetic nanomaterials for OA that target lesion sites, such as cartilage, subchondral bone, and synovium, through simulation of the physiological structure and microenvironment. Eventually, the challenges and prospects for the clinical translation of biomimetic nanomaterials are further discussed, with the goal of accessing an effective approach for OA treatment.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrodynamic Cavitation-Induced Thrombolysis on a Clot-on-a-Chip Platform

IF 4

Advanced Nanobiomed Research Pub Date : 2024-10-18 DOI: 10.1002/anbr.202400112

Beyzanur Ozogul, Unal Akar, Rabia Mercimek, Farzad Rokhsar Talabazar, Seyedali Seyedmirzaei Sarraf, Araz Sheibani Aghdam, Ali Ansari Hamedani, Luis Guillermo Villanueva, Dmitry Grishenkov, Ehsan Amani, Tugrul Elverdi, Morteza Ghorbani, Ali Koşar

{"title":"Hydrodynamic Cavitation-Induced Thrombolysis on a Clot-on-a-Chip Platform","authors":"Beyzanur Ozogul, Unal Akar, Rabia Mercimek, Farzad Rokhsar Talabazar, Seyedali Seyedmirzaei Sarraf, Araz Sheibani Aghdam, Ali Ansari Hamedani, Luis Guillermo Villanueva, Dmitry Grishenkov, Ehsan Amani, Tugrul Elverdi, Morteza Ghorbani, Ali Koşar","doi":"10.1002/anbr.202400112","DOIUrl":"https://doi.org/10.1002/anbr.202400112","url":null,"abstract":"\u0000Complications from thrombosis constitute a massive global burden for human health. Current treatment methods have limitations and can cause serious adverse effects. Hydrodynamic cavitation (HC) is a physical phenomenon where bubbles develop and collapse rapidly within a moving liquid due to sudden pressure changes. These collapsing bubbles provide high targeted energy which can be used in a controlled environment with the help of microfluidic devices. This study introduces a new clot-on-a-chip (CoC) platform based on HC, evaluated for thrombolysis efficacy. The microfluidic device, paired with a polydimethylsiloxane (PDMS) microchip, generates cavitation bubbles at low upstream pressures (≤482 kPa), enabling microscale blood clot erosion. Different HC exposure conditions (varying pressure and duration) are assessed by changes in clot mass, diameter, and scanning electron microscopy (SEM). The largest mass reduction occurs at 482 kPa for 120 s, with a decrease of 6.1 ± 0.12 mg, while the most erosion in diameter of blood clots is obtained 482 kPa for 120 s with complete removal. SEM results show increasing damage to clot structure from less to more intense HC exposures. The CoC platform, at controlled pressures and durations, efficiently disrupts clot structure and offers a promising drug-free alternative for thrombolysis treatment.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 1","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143116080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Nanomaterials in the Repair and Regeneration of Lymphatic Organs and Corresponding Biophysical Simulation Strategies

IF 4

Advanced Nanobiomed Research Pub Date : 2024-10-09 DOI: 10.1002/anbr.202400081

Bangheng Liu, Dong-An Wang

{"title":"Application of Nanomaterials in the Repair and Regeneration of Lymphatic Organs and Corresponding Biophysical Simulation Strategies","authors":"Bangheng Liu, Dong-An Wang","doi":"10.1002/anbr.202400081","DOIUrl":"https://doi.org/10.1002/anbr.202400081","url":null,"abstract":"Immune system diseases, malignant tumors, and traumatic injuries can directly damage the structure and function of lymphoid organs, while subsequent radiotherapy, chemotherapy, and lymph node dissection further damage the patient's immune system, leading to immune dysfunction, metabolic disorders, and increased susceptibility to infection, which seriously affect the patient's prognosis and quality of life. In this context, nanotechnology plays a key role in lymphoid organ regeneration and immune function recovery, including improving the therapeutic effect through targeted drug delivery systems, using targeted imaging probes to achieve tumor prediction and early detection, combining nanoplatforms with immunotherapy and photodynamic therapy to achieve synergistic therapeutic effects, and using nanomaterials to regulate the tumor microenvironment to enhance the sensitivity of traditional treatments. In addition, biophysical simulation strategies that simulate the microenvironment of lymphoid organs have also attracted widespread attention, aiming to construct a native cell environment to support the regeneration and functional recovery of damaged lymphoid tissues, or to simulate immune cells to regulate lymphocytes and induce specific immune responses. The multifaceted application of nanotechnology provides promising prospects for lymphoid organ regeneration and immune system repair.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 2","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface 模拟骨软骨界面的添加剂制造支架的研究进展

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400059

Ivo A. O. Beeren, Pieter J. Dijkstra, Carlos Mota, Sandra Camarero-Espinosa, Matthew B. Baker, Lorenzo Moroni

{"title":"Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface","authors":"Ivo A. O. Beeren, Pieter J. Dijkstra, Carlos Mota, Sandra Camarero-Espinosa, Matthew B. Baker, Lorenzo Moroni","doi":"10.1002/anbr.202400059","DOIUrl":"https://doi.org/10.1002/anbr.202400059","url":null,"abstract":"Architectural, compositional, and mechanical gradients are present in many interfacial tissues in the body. Yet desired for regeneration, the recreation of these complex natural gradients in porous scaffolds remains a challenging task. Additive manufacturing (AM) has been highlighted as a technology to fabricate constructs to regenerate interfacial tissues. Integration of different types of gradients, which can be physical, mechanical, and/or biochemical, shows promise to control cell fate and the regeneration process in a spatial controlled manner. One of the most studied tissue interfaces is the osteochondral unit which connects cartilage to bone. This tissue is often damaged because of trauma or ageing, leading to osteoarthritis; a degenerative disease and a major cause of disability worldwide. Therefore, in view of osteochondral (OC) regeneration, a state-of-the-art overview of current approaches is presented to manufacture gradient scaffolds prepared by AM techniques. The focus is on thermoplastic, hydrogel, and hybrid scaffolds comprising gradients that induce biomimicry by their physical and biological properties. The effect of these different systems on OC tissue formation in-vitro and in-vivo is addressed. Finally, an outlook on current trends of dynamic materials is provided, including proposals on how these materials could improve the mimicry of scaffolds applied for OC regeneration.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic Encapsulation of DNAs in Liquid Beads for Digital Loop-Mediated Isothermal Amplification 将 DNA 微流控封装在液体珠中用于数字环路介导等温扩增

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400044

Fariba Malekpour Galogahi, Simon Strachan, Ajeet Singh Yadav, Helen Stratton, Nam-Trung Nguyen

{"title":"Microfluidic Encapsulation of DNAs in Liquid Beads for Digital Loop-Mediated Isothermal Amplification","authors":"Fariba Malekpour Galogahi, Simon Strachan, Ajeet Singh Yadav, Helen Stratton, Nam-Trung Nguyen","doi":"10.1002/anbr.202400044","DOIUrl":"https://doi.org/10.1002/anbr.202400044","url":null,"abstract":"Digital nucleic acid analysis has emerged as a prominent tool for the detection and absolute quantification of diverse pathogens. Digital loop-mediated isothermal amplification (dLAMP) offers highly sensitive, specific, time-efficient, and cost-effective nucleic acid amplification. However, existing dLAMP techniques face challenges such as droplet merging, reliance on surfactants, restricted partition capacities, and the potential for sample loss during heating. Herein, these issues are addressed by introducing liquid beads for sample partitioning. Compared to microwells, our approach overcomes the limitations of chamber dimensions, enabling the analysis of an unlimited number of digitized targets. Furthermore, our novel approach effectively addresses sample loss and merging during thermal processing and eliminates the need for surfactants. Accurate and reproducible the quantitative detection of the gene cluster XALB1 of leaf scald disease is conducted using dLAMP based on liquid beads to verify its availability. The results demonstrate a high correlation between target concentration and positive signals, indicating the robust performance of our technique. A comparative analysis is then performed between dLAMP using liquid beads and using single droplets. Benchmarking these two techniques highlights the effectiveness of our innovative technique in overcoming existing challenges in dLAMP.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Human Amniotic Membrane Suspension Improves the Therapeutic Effect of Mesenchymal Stem Cells on Myocardial Infarction in Rats 新型人羊膜悬浮液改善了间充质干细胞对大鼠心肌梗死的治疗效果

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400084

Zhaoyi Li, Meirong Zhang, Yi Wang, Yijia Li, Yi Zhun Zhu

引用次数: 0

Engineering and Monitoring the Sustained Release of Extracellular Vesicles from Hydrogels for In Vivo Therapeutic Applications 为体内治疗应用设计并监测水凝胶中细胞外囊泡的持续释放

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-30 DOI: 10.1002/anbr.202400073

Selen Uman, Noah Weingarten, Mark Helmers, Amit Iyengar, Karen L. Xu, Kendra Worthington, Danika Meldrum, Jessica Dominic, Sara Guevara-Plunkett, Alexis Schiazza, Pavan Atluri, Jason A. Burdick

{"title":"Engineering and Monitoring the Sustained Release of Extracellular Vesicles from Hydrogels for In Vivo Therapeutic Applications","authors":"Selen Uman, Noah Weingarten, Mark Helmers, Amit Iyengar, Karen L. Xu, Kendra Worthington, Danika Meldrum, Jessica Dominic, Sara Guevara-Plunkett, Alexis Schiazza, Pavan Atluri, Jason A. Burdick","doi":"10.1002/anbr.202400073","DOIUrl":"https://doi.org/10.1002/anbr.202400073","url":null,"abstract":"Extracellular vesicles (EVs) are gaining interest in regenerative medicine and biomaterials have been shown to extend EV bioavailability following delivery. Herein, the labeling of both hydrogels and EVs is reported to better understand hydrogel design for sustained EV release into tissues. Shear-thinning hydrogels are engineered using guest–host (i.e., adamantane–cyclodextrin) modifications to hyaluronic acid (GH), as well as GH hydrogels with the addition of gelatin crosslinked via transglutaminase (GH+Gel) to temporally control hydrogel properties. When labeled with a near-IR dye and injected into rat myocardial tissue, the GH+Gel hydrogel is retained (>14 days) longer than the GH hydrogel alone (≈7 days), likely due to the added gelatin network. To overcome challenges associated with common EV labeling methods, a highly versatile metabolic labeling methodology is utilized via the incorporation of N-azidoacetylmannosamine-tetraacylated during EV synthesis to introduce azide groups that can then be reacted with DBCO dyes. When injected in saline, EVs are cleared within 24 h in hearts; however, hydrogels enhance EV retention, with levels based on hydrogel degradation behavior, namely, >14 days for GH+Gel hydrogel and ≈7 days for GH hydrogel alone. These findings support the use of hydrogels in EV therapies.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 11","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-Assembled Verteporfin Nanoparticles for Photodynamic and Light-Independent Therapy in Glioblastoma 用于胶质母细胞瘤光动力和光依赖疗法的自组装 Verteporfin 纳米粒子

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-28 DOI: 10.1002/anbr.202400098

John A. Quinlan, Kaylin Baumiller, Anandita Gaur, Wen-An Chiou, Robert W. Robey, Michael M. Gottesman, Huang-Chiao Huang

{"title":"Self-Assembled Verteporfin Nanoparticles for Photodynamic and Light-Independent Therapy in Glioblastoma","authors":"John A. Quinlan, Kaylin Baumiller, Anandita Gaur, Wen-An Chiou, Robert W. Robey, Michael M. Gottesman, Huang-Chiao Huang","doi":"10.1002/anbr.202400098","DOIUrl":"https://doi.org/10.1002/anbr.202400098","url":null,"abstract":"\u0000Verteporfin (VP) has been used for photodynamic therapy (PDT) for over 20 years, and new applications have brought it back into the spotlight. VP is hydrophobic and requires lipid carriers for clinical delivery as Visudyne. A nanosuspension of VP, termed NanoVP, that requires no carriers is developed, permitting delivery of VP alone in an aqueous solution. NanoVP is produced by solvent–antisolvent precipitation, with dimethyl sulfoxide as the preferable solvent of several screened. The initial formulation has a hydrodynamic diameter of 104 ± 6.0 nm, concentration of 133 ± 10 μm, polydispersity index (Pdi) of 0.12 ± 0.01, and zeta potential of −22.0 ± 0.93 mV. Seeking a concentration >500 μm, a zeta potential <−10 mV, a diameter <64 nm, and a Pdi < 0.2, eight synthesis parameters are probed, identifying three that modified nanoparticle diameter and three that modified nanoparticle dispersity. The diameter is tuned fourfold from 49.0 ± 4.4 to 195 ± 7.1 nm, and the solution concentration is increased by 6.3-fold to 838 ± 45.0 μm. Finally, the bioavailability and anticancer capacity of NanoVP in glioblastoma are evaluated. In all, this provides a framework for the modification of amorphous nanoparticle properties and a new formulation for clinical use of VP.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 12","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laponite Nanoclay-Loaded Microgel Suspensions as Supportive Matrices for Osteogenesis 皂石纳米粘土负载微凝胶悬浮液作为骨形成的支持性基质

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-11 DOI: 10.1002/anbr.202400024

Gagan K. Jalandhra, Tzong-tyng Hung, Kristopher A. Kilian

{"title":"Laponite Nanoclay-Loaded Microgel Suspensions as Supportive Matrices for Osteogenesis","authors":"Gagan K. Jalandhra, Tzong-tyng Hung, Kristopher A. Kilian","doi":"10.1002/anbr.202400024","DOIUrl":"https://doi.org/10.1002/anbr.202400024","url":null,"abstract":"Microscale carriers have emerged as promising materials for nurturing cell growth and as delivery vehicles for regenerative therapies. Carriers based on granular hydrogels have proved advantageous, where “microgels” can be formulated to have a broad range of properties to guide the behavior of adherent cells. Herein, the fabrication of osteogenic microgel matrices through the incorporation of laponite nanoclays is demonstrated. Forming a jammed suspension provides a scaffolding where cells can adhere to the surface of the microgels, with pathways for migration and proliferation fostered by the interstitial volume. By varying the content and type of laponite—RD and XLG—the degree of osteogenesis can be tuned in embedded populations of adipose-derived stem cells. The nano- and microstructured composite materials enhance osteogenesis at the transcript and protein level, leading to increased deposition of bone minerals and an increase in the compressive modulus of the assembled scaffold. Together, these microgel suspensions are promising materials for encouraging osteogenesis with scope for delivery via injection and stabilization to bone-mimetic mechanical properties after matrix deposition.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"4 10","pages":""},"PeriodicalIF":4.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioreactors: A Regenerative Approach to Skeletal Muscle Engineering for Repair and Replacement 生物反应器：用于修复和替代的骨骼肌工程再生方法

IF 4

Advanced Nanobiomed Research Pub Date : 2024-09-11 DOI: 10.1002/anbr.202400030

Alysha Williamson, Khashayar Khoshmanesh, Elena Pirogova, Peiqi Yang, Finn Snow, Richard Williams, Anita Quigley, Rob M. I. Kapsa

引用次数: 0