Advanced Nanobiomed Research最新文献_第4页

Multimaterial Triphasic Scaffold Replicates Enthesis Microenvironment to Modulate Early Cell Responses 多材料三相支架复制内置微环境调节早期细胞反应

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-06-01 Epub Date: 2026-02-22 DOI: 10.1002/anbr.202500249

Ginevra Pegollo, Simone Micalizzi, Girish Pattappa, Philipp Stahlhut, Jürgen Groll, Carmelo De Maria, Denitsa Docheva, Giovanni Vozzi

{"title":"Multimaterial Triphasic Scaffold Replicates Enthesis Microenvironment to Modulate Early Cell Responses","authors":"Ginevra Pegollo, Simone Micalizzi, Girish Pattappa, Philipp Stahlhut, Jürgen Groll, Carmelo De Maria, Denitsa Docheva, Giovanni Vozzi","doi":"10.1002/anbr.202500249","DOIUrl":"https://doi.org/10.1002/anbr.202500249","url":null,"abstract":"Current surgical approaches to reconstruct functional organization of tendon/ligament-to-bone interface, namely enthesis, often fail, highlighting the need for bioinspired tissue engineering strategies that replicate its native complexity. This study investigates the cellular response to a triphasic scaffold designed to mimic the enthesis gradient organization. The scaffold combines a fused deposition modelling polycaprolactone region mimicking bone, an electrospun poly(lactic-co-glycolic acid) (PLGA) region as tendon, with an overlap between the two to replicate enthesis. Human mesenchymal stem cells are seeded onto the scaffold, and their proliferation and morphology are assessed under static and mechanically stimulated conditions (two stimulation protocols) using a custom-designed bioreactor. Compared to static, in the first dynamic condition, cells align and elongate more along PLGA fibers, whilst the second protocol results in lesser elongation. Gene expression analysis shows moderate, but lineage-specific expression of tendon (SCXA, EGR1/2), cartilage (SOX9, ACAN), and bone (RUNX2, IBSP) markers. Tendon-related THBS2 is upregulated in the fibrocartilage- and tendon-like regions; moreover, the tendon maturation marker tenomodulin shows moderate expression in bone and tendon-like zones and upregulates under load in the fibrocartilage zone. These results demonstrate the potential of cyclic strain stimulation combined with the triphasic scaffold to spatially define cellular behaviors for enthesis tissue engineering.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148118049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Doxorubicin-Loaded Liposomes Baghdadite System for Localized Osteosarcoma Therapy and Bone Regeneration 多柔比星巴格达脂质体用于局部骨肉瘤治疗和骨再生

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-06-01 Epub Date: 2026-02-22 DOI: 10.1002/anbr.202500257

Sally Kortam, Zufu Lu, Iman Roohani, Ghazal Shineh, Leila Mamizadeh Janghour, Huu Nguyen Ngoc, Pooria Lesani, Behnam Akhavan, Hala Zreiqat

{"title":"A Doxorubicin-Loaded Liposomes Baghdadite System for Localized Osteosarcoma Therapy and Bone Regeneration","authors":"Sally Kortam, Zufu Lu, Iman Roohani, Ghazal Shineh, Leila Mamizadeh Janghour, Huu Nguyen Ngoc, Pooria Lesani, Behnam Akhavan, Hala Zreiqat","doi":"10.1002/anbr.202500257","DOIUrl":"https://doi.org/10.1002/anbr.202500257","url":null,"abstract":"Osteosarcoma (OS) is a primary malignant bone tumor often treated by surgical resection and systemic chemotherapy, which can cause severe side effects and nonspecific drug distribution. Localized drug delivery via bone scaffolds offers a promising alternative but faces limitations such as poor drug retention, instability of therapeutics, and insufficient mechanical strength for bone repair. This study presents a multifunctional implant integrating doxorubicin (DOX)-loaded liposomes into Baghdadite (Ca3ZrSi2O9, BAG) ceramics. Ion-assisted plasma polymer (IPP) coating was employed to enhance liposome immobilization and control DOX release. BAG functionalized with DOX-loaded liposomes (BAG/DOXlipo) significantly reduced OS cell viability, with enhanced efficacy observed in IPP-coated samples. Notably, BAG/DOXlipo (with or without IPP) showed no cytotoxicity to human osteoblasts, supported sustained ion release, and promoted alkaline phosphatase activity and bone mineralization. Additionally, the BAG/DOXlipo system demonstrated antibacterial activity against common implant-related pathogens. This platform uniquely combines mechanical robustness, osteoconductivity, and covalently bound liposomal chemotherapeutics to achieve localized tumor inhibition, bone regeneration, and infection control. It offers a promising strategy for OS treatment while minimizing systemic toxicity.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500257","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148118044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NUAK2 Inhibition Enhances Macromolecular Drug Delivery in a 3D Fibrotic Model of the Pancreatic Tumor Microenvironment NUAK2抑制增强胰腺肿瘤微环境三维纤维化模型中的大分子药物递送

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-06-01 Epub Date: 2026-02-24 DOI: 10.1002/anbr.202500237

Misaki Nakamura, Hiroyoshi Y. Tanaka, Mayu Ohira, Haruko Ohta-Okano, Reika Nakamura, Yu Seno, Saaya Yara, Sakura Fujita, Daichi Shibata, Masaya Yamamoto, Shinichi Toyooka, Kensuke Osada, Horacio Cabral, Atsushi Masamune, Mitsunobu R. Kano

{"title":"NUAK2 Inhibition Enhances Macromolecular Drug Delivery in a 3D Fibrotic Model of the Pancreatic Tumor Microenvironment","authors":"Misaki Nakamura, Hiroyoshi Y. Tanaka, Mayu Ohira, Haruko Ohta-Okano, Reika Nakamura, Yu Seno, Saaya Yara, Sakura Fujita, Daichi Shibata, Masaya Yamamoto, Shinichi Toyooka, Kensuke Osada, Horacio Cabral, Atsushi Masamune, Mitsunobu R. Kano","doi":"10.1002/anbr.202500237","DOIUrl":"https://doi.org/10.1002/anbr.202500237","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) features a fibrotic tumor microenvironment that impedes drug delivery and significantly limits the successful clinical application of nanomedicines. Targeting signaling in pancreatic stellate cells (PSCs), which drive fibrosis via excessive secretion of extracellular matrix proteins such as collagen I, may be useful in overcoming this fibrotic barrier. The AMPK-related kinases NUAK1/2 have recently gained interest as promoters of fibrosis, but whether they play a profibrotic role in PSCs remains unknown. Here, patient PSCs are used to assess NUAK1/2 involvement in the PDAC fibrotic barrier. Leveraging a 3D cell culture model of PDAC fibrosis, the effect of targeting NUAK1/2 on the permeability of macromolecular dextrans of various sizes, as well as physiologically relevant macromolecules, albumin and IgG, and clinical nanomedicines, Doxil and Abraxane, is investigated. NUAK1/2 inhibition is shown to diminish collagen I to enhance macromolecular permeability, via a mechanism independent of established pathways involving transforming growth factor-β (TGFβ) and yes-associated protein (YAP). Through isoform-specific knockdown, predominant NUAK2 involvement is demonstrated. Mechanistically, actin stress fiber regulation by NUAK2 is shown to be important. Altogether, these results show in vitro that NUAK2 promotes fibrotic signaling in PSCs and may be targeted to enhance macromolecular drug delivery in PDAC.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 6","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148120315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-27

引用次数: 0

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-27

引用次数: 0

Acoustofluidic Patterning for Improved Microtissue Histology 改进显微组织组织学的声流成像

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-04 Epub Date: 2026-01-20 DOI: 10.1002/anbr.202500079

Dhananjay V. Deshmukh, Emilie Vuille-dit-Bille, Saumitra Joshi, Nicola Gerber, Christine Fux, Annina Eichenberger, Epifania Bono, Markus Rimann, Sarah Heub, Daniel Migliozzi, Stephanie Boder-Pasche, Gilles Weder, Jurg Dual, Mark W. Tibbitt

{"title":"Acoustofluidic Patterning for Improved Microtissue Histology","authors":"Dhananjay V. Deshmukh, Emilie Vuille-dit-Bille, Saumitra Joshi, Nicola Gerber, Christine Fux, Annina Eichenberger, Epifania Bono, Markus Rimann, Sarah Heub, Daniel Migliozzi, Stephanie Boder-Pasche, Gilles Weder, Jurg Dual, Mark W. Tibbitt","doi":"10.1002/anbr.202500079","DOIUrl":"10.1002/anbr.202500079","url":null,"abstract":"Microtissues (such as organoids or spheroids) are useful to model healthy or diseased human tissues and organs, with application in drug testing, tissue engineering, and personalized medicine. To characterize their morphology and phenotype, microtissues are often monitored using histology and high-resolution imaging. While this technique is well-established and standard for macroscopic tissue sections, it remains challenging to execute and has low throughput in the context of these sub-millimeter structures, mainly due to the fact that their size makes them difficult to locate within the embedding medium. To enhance the efficiency of microtissue histology, an acoustofluidic device is developed to arrange microtissues in a coplanar manner within HistoGel, a widely used embedding medium. Prepatterning microtissues within HistoGel streamlines downstream processing by positioning the centers of mass of the microtissues at a predefined plane in the sample, simplifying sectioning, labeling, and imaging. The method is validated using differently sized HepG2 microtissues and tumor microtissues from osteosarcoma cells (Saos-2, MG-63, HOS), demonstrating that this method can be applied to different microtissue sizes and types, without modifying established postprocessing techniques. Our approach substantially enhances the microtissue histology workflow and broadens possibilities for microtissue analysis.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147683991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Silk on the Stabilization of Labile Enzyme Lipoxygenase 蚕丝对脂氧合酶稳定性的影响

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-04 Epub Date: 2025-12-30 DOI: 10.1002/anbr.202500182

Jacopo Giaretta, Xinyu (Vivian) Xie, Sandra Spoa, Hien A. Tran, Syamak Farajikhah, Sepehr Talebian, Jelena Rnjak-Kovacina, Fariba Dehghani

{"title":"The Effect of Silk on the Stabilization of Labile Enzyme Lipoxygenase","authors":"Jacopo Giaretta, Xinyu (Vivian) Xie, Sandra Spoa, Hien A. Tran, Syamak Farajikhah, Sepehr Talebian, Jelena Rnjak-Kovacina, Fariba Dehghani","doi":"10.1002/anbr.202500182","DOIUrl":"10.1002/anbr.202500182","url":null,"abstract":"Enzymes, as biological catalysts, are widely used for their excellent selectivity and sensitivity, playing a crucial role in biosensors, offering significant potential for monitoring food products and health conditions. However, enzyme activity can be compromised by environmental factors like high humidity, pH, and temperature variations. This study explores the feasibility of improving the stability of the unstable enzyme lipoxygenase (LOX) through immobilization using silk, a naturally occurring fibrous protein. A linoleic acid biosensor is designed by combining silk-entrapped LOX with poly(3,4-ethyloxy thiophene):poly(styrene sulfonate) (PEDOT:PSS) and horseradish peroxidase (HRP). The findings demonstrate that immobilizing LOX with different molecular weight silks extends the shelf-life of the freeze-dried PEDOT:PSS/HRP/LOX more than tenfold, from less than 1 day to 10 days. The results of the characterization tests (e.g., Fourier transform infrared spectroscopy, rheology) highlighted the interdependency between silk properties, such as its molecular weight, the silk conformation, and the pore shape and size, and enzyme stability. Low molecular weight silk resulted in the best performance, enabling storage at room temperature. It can therefore be concluded that immobilization with silk is an efficient method to enhance the shelf-life of enzyme-based biosensors, for diagnostic and other applications.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147684251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucin Glycoprotein Nanoparticles Enable a Selective Antisense Therapy for Oncogenic MicroRNAs 粘蛋白糖蛋白纳米颗粒可选择性反义治疗致癌microrna

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-04 Epub Date: 2026-01-04 DOI: 10.1002/anbr.202500210

Ceren Kimna, Thomas Mayer, Di Zhang, Matthias Marczynski, Carolin A. Rickert, Fabio Henkel, Lennart J. K. Weiss, Friedrich C. Simmel, Yuxia Luan, Oliver Lieleg

{"title":"Mucin Glycoprotein Nanoparticles Enable a Selective Antisense Therapy for Oncogenic MicroRNAs","authors":"Ceren Kimna, Thomas Mayer, Di Zhang, Matthias Marczynski, Carolin A. Rickert, Fabio Henkel, Lennart J. K. Weiss, Friedrich C. Simmel, Yuxia Luan, Oliver Lieleg","doi":"10.1002/anbr.202500210","DOIUrl":"10.1002/anbr.202500210","url":null,"abstract":"MicroRNAs (miRNAs) are critical regulators of gene expression, and their dysregulated expression has been implicated in the context of various diseases. Despite their therapeutic potential, the physicochemical instability and off-target effects of miRNA-targeting therapeutics pose significant challenges. To address this, the first antisense formulation is here developed based on highly biocompatible mucin glycoproteins, where the molecular cargo, synthetic DNA strands, has a dual functionality: stabilizing the structure of mucin glycoproteins into nanoparticles and enabling sequence-specific binding to oncogenic miRNA. These condensed nanoparticles leverage the intrinsic properties of mucins and can be modified with targeting ligands to enhance the specificity of cellular uptake. Findings demonstrate that mucin nanoparticles demonstrate therapeutic potential by inhibiting tumor growth in vivo. This presented approach not only enhances the specificity of nucleic acid delivery but also opens the door for the development of personalized diagnostics and therapies for diseases characterized by cell-specific overexpression of RNAs.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the Pathophysiology of Autoimmune Diseases—Mechanism, Triggers, and Nanotherapeutics: A Review 自身免疫性疾病的病理生理学——机制、触发因素和纳米治疗：综述

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-04 Epub Date: 2026-01-26 DOI: 10.1002/anbr.202500233

Md. Meraj Ansari, Yunhui Min, Eun-Ju Ko, Doyoung Kwon, Rehan Khan, Young-Ok Son

{"title":"Decoding the Pathophysiology of Autoimmune Diseases—Mechanism, Triggers, and Nanotherapeutics: A Review","authors":"Md. Meraj Ansari, Yunhui Min, Eun-Ju Ko, Doyoung Kwon, Rehan Khan, Young-Ok Son","doi":"10.1002/anbr.202500233","DOIUrl":"10.1002/anbr.202500233","url":null,"abstract":"Autoimmune diseases (AIDs) affect millions of individuals worldwide, impacting ≈10% of the population, with women being more susceptible to them than men. AIDs include multiple sclerosis (MS), type 1 diabetes (T1D), and rheumatoid arthritis (RA), which arise when the immune system unintentionally targets healthy cells. A complex interaction among immunological, environmental, and genetic factors causes AIDs by impairing the capacity of the immune system to discriminate between self- and non-self-antigens. This impairment makes the immune system of the body attack its own tissues, causing inflammation and damage. Gut microbiota, also referred to as the “forgotten organ,” is crucial for immune system control, with dysbiosis causing or worsening AIDs. Nanotechnology provides innovative approaches to targeted medicine delivery, reduction of adverse effects, and improved treatment efficacy, providing novel avenues for treating AIDs at nanoscale. Liposomes, hydrogels, and polymeric nanoparticles are used as drug carriers to directly deliver drugs to the impacted tissues. This technology increases treatment precision, while lowering systemic toxicity, guaranteeing localized and regulated drug release—an advantage valuable for long-term management of AIDs. In this comprehensive review, the pathophysiological connection among AIDs, role of gut microbiota, and combined nanotechnology-based management of these diseases are addressed.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147683961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanostructured Lipid Carriers Overcome the Low Immunogenicity of M2e Peptide via Surface Click Chemistry Conjugation, Improving the Anti-M2e Antibody Response 纳米结构脂质载体通过表面点击化学偶联克服M2e肽的低免疫原性，提高抗M2e抗体应答

IF 4.4

Advanced Nanobiomed Research Pub Date : 2026-04-04 Epub Date: 2026-02-15 DOI: 10.1002/anbr.202500236

Louis Bourlon, Carole Fournier, Antoine Hoang, Christine Charrat, Alban Lepetitcolin, Jessica Morlieras, Fabrice Navarro, Patrice N. Marche

{"title":"Nanostructured Lipid Carriers Overcome the Low Immunogenicity of M2e Peptide via Surface Click Chemistry Conjugation, Improving the Anti-M2e Antibody Response","authors":"Louis Bourlon, Carole Fournier, Antoine Hoang, Christine Charrat, Alban Lepetitcolin, Jessica Morlieras, Fabrice Navarro, Patrice N. Marche","doi":"10.1002/anbr.202500236","DOIUrl":"10.1002/anbr.202500236","url":null,"abstract":"Influenza vaccines targeting hemagglutinin require annual updates due to viral variability, limiting protection against pandemic strains. The conserved matrix protein 2 ectodomain (M2e) peptide offers cross-strain immunity but suffers from low immunogenicity. Here, M2e peptides are conjugated onto nanostructured lipid carriers (NLCs) using bioorthogonal click chemistry, preserving key epitopes and enhancing antigen presentation. Immunization in mice demonstrates that NLC-M2e induces focused and robust anti-M2e antibody responses, which are significantly amplified by incorporating a pan-DR epitope helper T cell epitope, achieving antibody levels compatible with protection. The formulation elicits balanced IgG subclasses and T-helper 1 cell-type cellular responses, supporting effective immune mechanisms. These findings suggest that NLC-based delivery of M2e peptides, especially with helper epitopes, represents a promising universal influenza vaccine strategy warranting further preclinical and clinical investigation.","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"6 4","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://advanced.onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202500236","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0