Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel
{"title":"Inhibition of Biofilm Formation on Orthopedic Implants Based on Spider Silk Coatings Increases Survival of Galleria mellonella","authors":"Supun Mohotti, Gopala K. Mannala, Hendrik Bargel, Volker Alt, Thomas Scheibel","doi":"10.1002/anbr.202400160","DOIUrl":null,"url":null,"abstract":"<p>\nThe microbial repellence of some spider silk-based materials makes them interesting candidates for biomedical applications. This study investigates the microbial repellent properties of recombinant spider silk coatings on orthopedic metal implants, specifically targeting the prevention of biofilm-related implant infections caused by multidrug-resistant bacteria such as <i>Staphylococcus aureus</i>. Utilizing <i>Galleria mellonella</i> as an in vivo model, stainless steel and titanium implants coated with films made of three different recombinant spider silk proteins are analyzed concerning biofilm formation and its impact on animal survival. Amongst the tested spider silk variants, the polyanionic eADF4(C16) demonstrates superior bacterial-repellent properties and improved larval survivability. Scanning electron microscopy analysis reveals reduced bacterial presence on eADF4(C16)-coated wires compared to uncoated controls, correlating with survival data. Based on the results, the potential of recombinant spider silk coatings to enhance implant functionality and longevity is highlighted, presenting a novel solution to combat biofilm-related implant infections and address the growing threat of antimicrobial resistance. Furthermore, employing <i>Galleria mellonella</i> as an in vivo model underscores a commitment to ethical research practices in studying biofilm infections.</p>","PeriodicalId":29975,"journal":{"name":"Advanced Nanobiomed Research","volume":"5 4","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anbr.202400160","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Nanobiomed Research","FirstCategoryId":"1085","ListUrlMain":"https://advanced.onlinelibrary.wiley.com/doi/10.1002/anbr.202400160","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The microbial repellence of some spider silk-based materials makes them interesting candidates for biomedical applications. This study investigates the microbial repellent properties of recombinant spider silk coatings on orthopedic metal implants, specifically targeting the prevention of biofilm-related implant infections caused by multidrug-resistant bacteria such as Staphylococcus aureus. Utilizing Galleria mellonella as an in vivo model, stainless steel and titanium implants coated with films made of three different recombinant spider silk proteins are analyzed concerning biofilm formation and its impact on animal survival. Amongst the tested spider silk variants, the polyanionic eADF4(C16) demonstrates superior bacterial-repellent properties and improved larval survivability. Scanning electron microscopy analysis reveals reduced bacterial presence on eADF4(C16)-coated wires compared to uncoated controls, correlating with survival data. Based on the results, the potential of recombinant spider silk coatings to enhance implant functionality and longevity is highlighted, presenting a novel solution to combat biofilm-related implant infections and address the growing threat of antimicrobial resistance. Furthermore, employing Galleria mellonella as an in vivo model underscores a commitment to ethical research practices in studying biofilm infections.
期刊介绍：
Advanced NanoBiomed Research will provide an Open Access home for cutting-edge nanomedicine, bioengineering and biomaterials research aimed at improving human health. The journal will capture a broad spectrum of research from increasingly multi- and interdisciplinary fields of the traditional areas of biomedicine, bioengineering and health-related materials science as well as precision and personalized medicine, drug delivery, and artificial intelligence-driven health science.
The scope of Advanced NanoBiomed Research will cover the following key subject areas:
▪ Nanomedicine and nanotechnology, with applications in drug and gene delivery, diagnostics, theranostics, photothermal and photodynamic therapy and multimodal imaging.
▪ Biomaterials, including hydrogels, 2D materials, biopolymers, composites, biodegradable materials, biohybrids and biomimetics (such as artificial cells, exosomes and extracellular vesicles), as well as all organic and inorganic materials for biomedical applications.
▪ Biointerfaces, such as anti-microbial surfaces and coatings, as well as interfaces for cellular engineering, immunoengineering and 3D cell culture.
▪ Biofabrication including (bio)inks and technologies, towards generation of functional tissues and organs.
▪ Tissue engineering and regenerative medicine, including scaffolds and scaffold-free approaches, for bone, ligament, muscle, skin, neural, cardiac tissue engineering and tissue vascularization.
▪ Devices for healthcare applications, disease modelling and treatment, such as diagnostics, lab-on-a-chip, organs-on-a-chip, bioMEMS, bioelectronics, wearables, actuators, soft robotics, and intelligent drug delivery systems.
with a strong focus on applications of these fields, from bench-to-bedside, for treatment of all diseases and disorders, such as infectious, autoimmune, cardiovascular and metabolic diseases, neurological disorders and cancer; including pharmacology and toxicology studies.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
