Small Science最新文献_第8页

m6A Ribonucleic Acid Methylation in Fibrotic Diseases of Visceral Organs. 内脏器官纤维化疾病中的m6A核糖核酸甲基化。

IF 11.1

Small Science Pub Date : 2024-11-21 eCollection Date: 2025-02-01 DOI: 10.1002/smsc.202400308

Xiaoniu Dai, Yusi Cheng, Wei Luo, Jing Wang, Cuifen Wang, Xinxin Zhang, Wei Zhang, Jie Chao

{"title":"m6A Ribonucleic Acid Methylation in Fibrotic Diseases of Visceral Organs.","authors":"Xiaoniu Dai, Yusi Cheng, Wei Luo, Jing Wang, Cuifen Wang, Xinxin Zhang, Wei Zhang, Jie Chao","doi":"10.1002/smsc.202400308","DOIUrl":"https://doi.org/10.1002/smsc.202400308","url":null,"abstract":"Fibrosis is a pathological process characterized by the excessive deposition of extracellular matrix in the tissue's extracellular space, leading to structural injury and organ dysfunction, and even organ failure, posing a threat to human life. Despite mounting evidence suggesting that fibrosis is reversible, effective treatments for fibrotic diseases are lacking. Accumulating evidence has elucidated that ribonucleic acid (RNA) modifications have emerged as novel mechanisms regulating gene expression. N6-methyladenosine (m6A) modification is a well-known prevalent RNA posttranscriptional modification that participates in essential biological processes such as RNA splicing, translation, and degradation. It is tightly implicated in a wide range of cellular processes and various human diseases, particularly in organ fibrosis. The m6A modification is a dynamic and reversible process regulated by methylases, commonly known as \"writers,\" and demethylases referred to as \"erasers,\" while m6A modifications are recognized by \"readers.\" Accumulating evidence suggests that m6A modification on RNAs is tightly associated with fibrotic diseases of visceral organs including the lungs, heart, liver, and kidney. In this review, recent advances in the impact of m6A methylation of RNAs on visceral organ fibrosis are highlighted and the potential prospects for therapy in treating fibrotic diseases of visceral organs are discussed.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 2","pages":"2400308"},"PeriodicalIF":11.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol-Mediated Multifunctional Human-Machine Interface Hydrogel Electrodes in Bioelectronics. 生物电子学中多酚介导的多功能人机界面水凝胶电极。

IF 11.1

Small Science Pub Date : 2024-11-21 eCollection Date: 2025-01-01 DOI: 10.1002/smsc.202400362

Lili Jiang, Donglin Gan, Chuangyi Xu, Tingting Zhang, Mingyuan Gao, Chaoming Xie, Denghui Zhang, Xiong Lu

{"title":"Polyphenol-Mediated Multifunctional Human-Machine Interface Hydrogel Electrodes in Bioelectronics.","authors":"Lili Jiang, Donglin Gan, Chuangyi Xu, Tingting Zhang, Mingyuan Gao, Chaoming Xie, Denghui Zhang, Xiong Lu","doi":"10.1002/smsc.202400362","DOIUrl":"https://doi.org/10.1002/smsc.202400362","url":null,"abstract":"Human-machine interface (HMI) electrodes enable interactions between humans and bioelectronic devices by facilitating electrical stimulation and recording neural activity. However, reconciling the soft, hydrated nature of living human tissues with the rigid, dry properties of synthetic electronic systems is inherently challenging. Overcoming these significant differences, which is critical for developing compatible, effective, and stable interfaces, has become a key research area in materials science and technology. Recently, hydrogels have gained prominence for use in HMI electrodes because these soft, hydrated materials are similar in nature to human tissues and can be tuned through the incorporation of nanofillers. This review examines the functional requirements of HMI electrodes and highlights recent progress in the development of polyphenol-mediated multifunctional hydrogel-based HMI electrodes for bioelectronics. Furthermore, aspects such as mussel-inspired and polyphenol-mediated adhesion, underlying mechanisms, tissue-matching mechanical properties, electrochemical performance, biocompatibility, biofouling resistance, stability under physiological conditions, anti-inflammatory, and antioxidant properties are discussed. Finally, applications in bioelectronics and further perspectives are outlined. Advances in HMI hydrogel electrodes are expected to facilitate the unprecedented integration of biological systems and electronic devices, potentially revolutionizing various biomedical fields and enhancing the capabilities and performance of bioelectronic devices.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 1","pages":"2400362"},"PeriodicalIF":11.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micropatterning of Confined Surfaces with Polymer Brushes by Two-Photon-Initiated Reversible Addition-Fragmentation Chain-Transfer Polymerization. 双光子引发的可逆加成-破碎-链转移聚合聚合物刷在受限表面的微图像化。

IF 11.1

Small Science Pub Date : 2024-11-21 eCollection Date: 2025-01-01 DOI: 10.1002/smsc.202400263

Stefan Helfert, Tommaso Zandrini, Andreas Rohatschek, Manuel Rufin, Peter Machata, Anna Zahoranová, Orestis G Andriotis, Philipp J Thurner, Aleksandr Ovsianikov, Robert Liska, Stefan Baudis

{"title":"Micropatterning of Confined Surfaces with Polymer Brushes by Two-Photon-Initiated Reversible Addition-Fragmentation Chain-Transfer Polymerization.","authors":"Stefan Helfert, Tommaso Zandrini, Andreas Rohatschek, Manuel Rufin, Peter Machata, Anna Zahoranová, Orestis G Andriotis, Philipp J Thurner, Aleksandr Ovsianikov, Robert Liska, Stefan Baudis","doi":"10.1002/smsc.202400263","DOIUrl":"https://doi.org/10.1002/smsc.202400263","url":null,"abstract":"Photopatterned polymer brushes provide a viable option to alter the surface properties of biosensors, substrates for tissue engineering, or microelectronic implants. Although the one-photon direct laser writing enables excellent control over pattern geometry, it has an inherently limited writing resolution caused by the used light source; moreover, no patterning of undercuts or channels is possible. This article describes the preparation of patterned polymer brushes on confined glass substrates using two-photon-initiated reversible addition-fragmentation chain-transfer (2PRAFT) polymerization of N-acryloylmorpholine as a hydrophilic model monomer. The polymer brushes prepared by 2PRAFT exhibit a height of 10 nm, as confirmed by atomic force microscopy. In addition, well-defined printed structures down to 5 μm size are prepared, which outperforms the currently achieved resolution of polymer brushes prepared by one-photon direct laser writing.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 1","pages":"2400263"},"PeriodicalIF":11.1,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dip-Pen Nanolithography-Based Fabrication of Meta-Chemical Surface for Heavy Metal Detection: Role of Poly-Methyl Methacrylate in Sensor Sensitivity. 基于浸笔纳米光刻的重金属检测元化学表面制备：聚甲基丙烯酸甲酯在传感器灵敏度中的作用。

IF 11.1

Small Science Pub Date : 2024-11-20 eCollection Date: 2025-02-01 DOI: 10.1002/smsc.202400459

Rahma Okbi, Mohammed Alkrenawi, Krishna Kumar Yadav, Dror Shamir, Haya Kornweitz, Yael Peled, Moshe Zohar, Ariela Burg

{"title":"Dip-Pen Nanolithography-Based Fabrication of Meta-Chemical Surface for Heavy Metal Detection: Role of Poly-Methyl Methacrylate in Sensor Sensitivity.","authors":"Rahma Okbi, Mohammed Alkrenawi, Krishna Kumar Yadav, Dror Shamir, Haya Kornweitz, Yael Peled, Moshe Zohar, Ariela Burg","doi":"10.1002/smsc.202400459","DOIUrl":"https://doi.org/10.1002/smsc.202400459","url":null,"abstract":"A meta-chemical surface is being patterned via dip-pen nanolithography (DPN) for novel electrochemical heavy metal sensors. The unique feature of DPN allows a precise transfer of desired ink onto various surfaces. Two kinds of sensors are being developed, which differ by the ligand in the poly-methyl methacrylate (PMMA)-based ink: 1,8-diaminonaphthalene (DAN) and D-penicillamine (D-PA). The nanosize, the surface-to-volume ratio (18.6 and 23.1 μm-1 for DAN- and D-PA-based ink, respectively), and the binding strength between the ligand and the cation (2.21 and -21.37 kcal mol-1 for DAN- and D-PA-based ink, respectively) are found to be the source of their high sensitivity, with limit of detection values of 0.40 and 0.30 ppb for DAN and D-PA, respectively. According to the DFT calculations, the binding reactions in the presence of PMMA are more exergonic; this indicates that PMMA added to the ink for the patterning process improves the binding between the metals and the ligands. This enhanced binding between the metals and the ligands is a crucial and innovative function of the PMMA that can enhance sensor performance.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 2","pages":"2400459"},"PeriodicalIF":11.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Free-Base Octaethylporphyrin on Au(111) as Heterogeneous Organic Molecular Electrocatalyst for Oxygen Reduction Reaction in Acid Media: An Electrochemical Scanning Tunneling Microscopy and Rotating Ring-Disc Electrode Analyses. 金（111）上的游离基八乙基卟啉作为酸性介质中氧还原反应的非均相有机分子电催化剂：电化学扫描隧道显微镜和旋转环盘电极分析。

IF 11.1

Small Science Pub Date : 2024-11-19 eCollection Date: 2025-01-01 DOI: 10.1002/smsc.202400294

Francesco Cazzadori, Alessandro Facchin, Silvio Reginato, Daniel Forrer, Christian Durante

{"title":"Free-Base Octaethylporphyrin on Au(111) as Heterogeneous Organic Molecular Electrocatalyst for Oxygen Reduction Reaction in Acid Media: An Electrochemical Scanning Tunneling Microscopy and Rotating Ring-Disc Electrode Analyses.","authors":"Francesco Cazzadori, Alessandro Facchin, Silvio Reginato, Daniel Forrer, Christian Durante","doi":"10.1002/smsc.202400294","DOIUrl":"https://doi.org/10.1002/smsc.202400294","url":null,"abstract":"The oxygen reduction reaction (ORR) using metal porphyrin catalysts is currently widely explored. Conversely, metal-free molecular systems are much less investigated, and there is limited information available for molecules such as nonmetalated macrocycles capable of catalyzing the ORR or other small molecules. Herein, the activity and selectivity of a heterogeneous organic molecular electrocatalyst, octaethylporphyrin (H2OEP), adsorbed on Au(111) toward ORR in acidic aqueous electrolyte are investigated. Electrochemical scanning tunneling microscopy (EC-STM) is employed to monitor the molecular layer during the electrochemical process. Additionally, cyclic and linear sweep voltammetries are performed at still and rotating Pt/ring-H2OEP-functionalized Au(111)/disk electrodes to determine the activity and selectivity of the H2OEP monolayer toward ORR on Au(111). Based on EC-STM and computation analysis, dioxygen electroreduction does not follow an inner-sphere electron transfer reduction as seen in metal porphyrins, where a preliminary M-O2 bond has to form, but it follows an outer-sphere mechanism involving the precoordination of O2 induced by the protonated hydrogen of the macrocycle cavity.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 1","pages":"2400294"},"PeriodicalIF":11.1,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Extracellular Vesicles in Viscous Formulations: Interplay of Nanoparticle Tracking and Nanorheology via Interferometric Light Microscopy. 研究粘性配方中的细胞外囊泡：通过干涉光学显微镜观察纳米颗粒跟踪和纳米流变学的相互作用。

IF 11.1

Small Science Pub Date : 2024-11-18 eCollection Date: 2025-01-01 DOI: 10.1002/smsc.202400319

Lucile Alexandre, Anastasiia Dubrova, Aruna Kunduru, Estelle Surply, Christopher Ribes, Imane Boucenna, Florence Gazeau, Amanda K A Silva, Stéphanie Mangenot, Kelly Aubertin

{"title":"Investigating Extracellular Vesicles in Viscous Formulations: Interplay of Nanoparticle Tracking and Nanorheology via Interferometric Light Microscopy.","authors":"Lucile Alexandre, Anastasiia Dubrova, Aruna Kunduru, Estelle Surply, Christopher Ribes, Imane Boucenna, Florence Gazeau, Amanda K A Silva, Stéphanie Mangenot, Kelly Aubertin","doi":"10.1002/smsc.202400319","DOIUrl":"https://doi.org/10.1002/smsc.202400319","url":null,"abstract":"While extracellular vesicles (EVs) demonstrate growing potential as innovative cell-derived nanobiotherapies in diverse medical contexts, their physical properties (size, integrity, transport, etc.) in drug product formulation remain a critical concern poorly addressed so far. Herein, a methodology that relies on nanoparticle tracking analysis by interferometric light microscopy (ILM) for analyzing the concentration and size distribution of nanoparticles as well as their interactions with their local environment through a nanorheological approach is introduced. The analysis of interference patterns enables nanoparticles tracking not only in aqueous solutions but also in complex media with high-viscosity or non-Newtonian behavior, particularly pertinent for characterizing EV formulations. A proof of concept for in situ tracking of EVs suspended in Poloxamer-407 as drug delivery system is presented. The ILM-based analysis enables to 1) measure the viscosity at the nanoscale for Newtonian and non-Newtonian fluids via calibration beads; 2) analyze data to determine the size distribution of EVs in non-Newtonian complex fluid such as poloxamer formulation, and 3) analyze the interactions of EVs with poloxamer-407. The proposed approach represents a valuable tool to understand the nanorheological behavior of EVs in viscoelastic media in situ as well as a quality control test for EV formulations intended to clinical use.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 1","pages":"2400319"},"PeriodicalIF":11.1,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleation-Controlled Doping of II-VI Semiconductor Nanocrystals Mediated by Magic-Sized Clusters. magic - size团簇介导的II-VI半导体纳米晶体的核控掺杂。

IF 11.1

Small Science Pub Date : 2024-11-15 eCollection Date: 2025-01-01 DOI: 10.1002/smsc.202400300

Seunghyun Ji, Hafiz Ghulam Abbas, Seo Young Kim, Hyo Cheol Lee, Kyunghoon Lee, Shi Li, Seungho Choe, Hyungju Ahn, Stefan Ringe, Jiwoong Yang

{"title":"Nucleation-Controlled Doping of II-VI Semiconductor Nanocrystals Mediated by Magic-Sized Clusters.","authors":"Seunghyun Ji, Hafiz Ghulam Abbas, Seo Young Kim, Hyo Cheol Lee, Kyunghoon Lee, Shi Li, Seungho Choe, Hyungju Ahn, Stefan Ringe, Jiwoong Yang","doi":"10.1002/smsc.202400300","DOIUrl":"https://doi.org/10.1002/smsc.202400300","url":null,"abstract":"Doping quantum-confined semiconductor nanocrystals offers an effective way to tailor their unique properties. However, the inherent challenges of nanoscale doping processes, such as the low probability of successful doping, have hindered their practical applications. Nucleation-controlled doping has emerged as a potential solution, but a comprehensive mechanistic understanding of this process is lacking. Herein, the nucleation-controlled doping process facilitated by magic-sized cluster intermediates is elucidated. This approach enables the synthesis of 2D ZnSe quantum nanoribbons with two distinct doping sites. Remarkably, the identity of the dopants plays a critical role in determining the chemical pathways of nucleation-controlled doping. Substitutional doping of magic-sized clusters with Mn2+ ions leads to successful substitutional doping of the final 2D nanocrystals. Conversely, Co2+ ions, initially occupying substitutional positions in the magic-sized cluster intermediates, relocate to alternative sites, such as interstitial sites, in the final nanocrystals. First-principle calculations of dopant formation energies support these experimental findings, demonstrating the thermodynamic favorability of specific dopant site preferences. Moreover, a consistent tendency is observed in CdSe nanocrystals, suggesting that the proposed doping mechanism is generally applicable to II-VI semiconductors. This study will advance the controlled synthesis of various doped semiconductor nanocrystals using nucleation-controlled doping processes.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 1","pages":"2400300"},"PeriodicalIF":11.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maximizing Bifunctionality for Overall Water Splitting by Integrating H₂ Spillover and Oxygen Vacancies in CoPBO/Co₃O₄ Composite Catalyst. 利用cobo /Co3O4复合催化剂中H2溢出和氧空位的集成最大化水整体分解的双功能。

IF 11.1

Small Science Pub Date : 2024-11-10 eCollection Date: 2024-12-01 DOI: 10.1002/smsc.202400343

Rinkoo Bhabal, Aniruddha Bhide, Suraj Gupta, Rohan Fernandes, Nainesh Patel

{"title":"Maximizing Bifunctionality for Overall Water Splitting by Integrating H2 Spillover and Oxygen Vacancies in CoPBO/Co3O4 Composite Catalyst.","authors":"Rinkoo Bhabal, Aniruddha Bhide, Suraj Gupta, Rohan Fernandes, Nainesh Patel","doi":"10.1002/smsc.202400343","DOIUrl":"https://doi.org/10.1002/smsc.202400343","url":null,"abstract":"In the pursuit of utilizing renewable energy sources for green hydrogen (H2) production, alkaline water electrolysis has emerged as a key technology. To improve the reaction rates of overall water electrolysis and simplify electrode manufacturing, development of bifunctional electrocatalysts is of great relevance. Herein, CoPBO/Co3O4 is reported as a binary composite catalyst comprising amorphous (CoPBO) and crystalline (Co3O4) phases as a high-performing bifunctional electrocatalyst for alkaline water electrolysis. Owing to the peculiar properties of CoPBO and Co3O4, such as complementing Gibbs free energy values for H-adsorption (ΔG H) and relatively smaller difference in their work functions (ΔΦ), the composite exhibits H2 spillover (HS) mechanism to facilitate the hydrogen evolution reaction (HER). The outcome is manifested in the form of a low HER overpotential of 65 mV (at 10 mA cm-2). Moreover, an abundant amount of surface oxygen vacancies (Ov) are observed in the same CoPBO/Co3O4 composite that facilitates oxygen evolution reaction (OER) as well, leading to a mere 270 mV OER overpotential (at 10 mA cm-2). The present work showcases the possibilities to strategically design non-noble composite catalysts that combine the advantages of HS phenomenon as well as Ov to achieve new record performances in alkaline water electrolysis.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"4 12","pages":"2400343"},"PeriodicalIF":11.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and Long-Term Super-Resolution Imaging of Endoplasmic Reticulum Nano-structural Dynamics in Living Cells Using a Neural Network. 使用神经网络的活细胞内质网纳米结构动态的快速和长期超分辨率成像。

IF 11.1

Small Science Pub Date : 2024-11-08 eCollection Date: 2025-01-01 DOI: 10.1002/smsc.202400385

Johanna V Rahm, Ashwin Balakrishnan, Maren Wehrheim, Alexandra Kaminer, Marius Glogger, Laurell F Kessler, Matthias Kaschube, Hans-Dieter Barth, Mike Heilemann

{"title":"Fast and Long-Term Super-Resolution Imaging of Endoplasmic Reticulum Nano-structural Dynamics in Living Cells Using a Neural Network.","authors":"Johanna V Rahm, Ashwin Balakrishnan, Maren Wehrheim, Alexandra Kaminer, Marius Glogger, Laurell F Kessler, Matthias Kaschube, Hans-Dieter Barth, Mike Heilemann","doi":"10.1002/smsc.202400385","DOIUrl":"https://doi.org/10.1002/smsc.202400385","url":null,"abstract":"Stimulated emission depletion (STED) microscopy is a super-resolution technique that surpasses the diffraction limit and has contributed to the study of dynamic processes in living cells. However, high laser intensities induce fluorophore photobleaching and sample phototoxicity, limiting the number of fluorescence images obtainable from a living cell. Herein, these challenges are addressed by using ultra-low irradiation intensities and a neural network for image restoration, enabling extensive imaging of single living cells. The endoplasmic reticulum (ER) is chosen as the target structure due to its dynamic nature over short and long timescales. The reduced irradiation intensity combined with denoising permits continuous ER dynamics observation in living cells for up to 7 h with a temporal resolution of seconds. This allows for quantitative analysis of ER structural features over short (seconds) and long (hours) timescales within the same cell, and enabled fast 3D live-cell STED microscopy. Overall, the combination of ultralow irradiation with image restoration enables comprehensive analysis of organelle dynamics over extended periods in living cells.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 1","pages":"2400385"},"PeriodicalIF":11.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Light to Logic: Recent Advances in Optoelectronic Logic Gate. 从光到逻辑：光电逻辑门的最新进展。

IF 11.1

Small Science Pub Date : 2024-11-03 eCollection Date: 2024-12-01 DOI: 10.1002/smsc.202400264

Woochul Kim, Dante Ahn, Minz Lee, Namsoo Lim, Hyeonghun Kim, Yusin Pak

{"title":"From Light to Logic: Recent Advances in Optoelectronic Logic Gate.","authors":"Woochul Kim, Dante Ahn, Minz Lee, Namsoo Lim, Hyeonghun Kim, Yusin Pak","doi":"10.1002/smsc.202400264","DOIUrl":"https://doi.org/10.1002/smsc.202400264","url":null,"abstract":"This review delves into the advancements in optoelectronic logic gate (OELG) devices, emphasizing their transformative potential in computational technology through the integration of optical and electronic components. OELGs present significant advantages over traditional electronic logic gates, including enhanced processing speed, bandwidth, and energy efficiency. The evolution of OELG architectures from single-device, single-logic systems to more sophisticated multidevice, multilogic, and reconfigurable OELGs is comprehensively explored. Key advancements include the development of materials and device structures enabling multifunctional logic operations and the incorporation of in-memory functionalities, critical for applications in high-performance computing and real-time data processing. This review also addresses the challenges that need to be overcome, such as stability, durability, integration with existing semiconductor technologies, and efficiency. By summarizing current research and proposing future directions, this review aims to guide the ongoing development of next-generation optoelectronic architectures, poised to redefine the landscape of optical computing, communication, and data processing.","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"4 12","pages":"2400264"},"PeriodicalIF":11.1,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11935027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0