Current Opinion in Chemical Biology最新文献_第7页

Multinuclear non-heme iron dependent oxidative enzymes (MNIOs) involved in unusual peptide modifications 参与异常肽修饰的多核非血红素铁依赖性氧化酶（MNIOs）

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-20 DOI: 10.1016/j.cbpa.2024.102467

Jeff Y. Chen, Wilfred A. van der Donk

{"title":"Multinuclear non-heme iron dependent oxidative enzymes (MNIOs) involved in unusual peptide modifications","authors":"Jeff Y. Chen, Wilfred A. van der Donk","doi":"10.1016/j.cbpa.2024.102467","DOIUrl":"https://doi.org/10.1016/j.cbpa.2024.102467","url":null,"abstract":"<div><p>Multinuclear non-heme iron dependent oxidative enzymes (MNIOs), formerly known as domain of unknown function 692 (DUF692), are involved in the post-translational modification of peptides during the biosynthesis of peptide-based natural products. These enzymes catalyze highly unusual and diverse chemical modifications. Several class-defining features of this large family (>14 000 members) are beginning to emerge. Structurally, the enzymes are characterized by a TIM-barrel fold and a set of conserved residues for a di- or tri–iron binding site. They use molecular oxygen to modify peptide substrates, often in a four-electron oxidation taking place at a cysteine residue. This review summarizes the current understanding of MNIOs. Four modifications are discussed in detail: oxazolone-thioamide formation, β-carbon excision, hydantoin-macrocycle formation, and 5-thiooxazole formation. Briefly discussed are two other reactions that do not take place on Cys residues.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"80 ","pages":"Article 102467"},"PeriodicalIF":7.8,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000437/pdfft?md5=ff0d2067bb16ebe523993dbaeab8b350&pid=1-s2.0-S1367593124000437-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New analytical methods focusing on polar metabolite analysis in mass spectrometry and NMR-based metabolomics 以质谱和核磁共振为基础的代谢组学中极性代谢物分析为重点的新分析方法

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-20 DOI: 10.1016/j.cbpa.2024.102466

James McCullagh, Fay Probert

引用次数: 0

Penicillin-binding protein-type thioesterases: An emerging family of non-ribosomal peptide cyclases with biocatalytic potentials 青霉素结合蛋白型硫酯酶：具有生物催化潜力的新兴非核糖体肽环酶家族

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-17 DOI: 10.1016/j.cbpa.2024.102465

Kenichi Matsuda, Toshiyuki Wakimoto

引用次数: 0

Oxygen-transfer reactions by enzymatic flavin-N5 oxygen adducts—Oxidation is not a must 酶促黄素-N5 氧加合物的氧转移反应--氧化并非必要条件

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-12 DOI: 10.1016/j.cbpa.2024.102464

Robin Teufel

{"title":"Oxygen-transfer reactions by enzymatic flavin-N5 oxygen adducts—Oxidation is not a must","authors":"Robin Teufel","doi":"10.1016/j.cbpa.2024.102464","DOIUrl":"https://doi.org/10.1016/j.cbpa.2024.102464","url":null,"abstract":"<div><p>Flavoenzymes catalyze numerous redox reactions including the transfer of an O<sub>2</sub>-derived oxygen atom to organic substrates, while the other one is reduced to water. Investigation of some of these monooxygenases led to a detailed understanding of their catalytic cycle, which involves the flavin-C<sub>4α</sub>-(hydro)peroxide as hallmark oxygenating species, and newly discovered flavoprotein monooxygenases were generally assumed to operate similarly. However, discoveries in recent years revealed a broader mechanistic versatility, including enzymes that utilize flavin-N<sub>5</sub> oxygen adducts for catalysis in the form of the flavin-N<sub>5</sub>-(hydro)peroxide and the flavin-N<sub>5</sub>-oxide species. In this review, I will highlight recent developments in that area, including noncanonical flavoenzymes from natural product biosynthesis and sulfur metabolism that provide first insights into the chemical properties of these species. Remarkably, some enzymes may even combine the flavin-N<sub>5</sub>-peroxide and the flavin-N<sub>5</sub>-oxide species for consecutive oxygen-transfers to the same substrate and thereby in essence operate as dioxygenases.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"80 ","pages":"Article 102464"},"PeriodicalIF":7.8,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000401/pdfft?md5=8da170b60e8a1b645e526e1bec5a4df5&pid=1-s2.0-S1367593124000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel types of RiPP-modifying enzymes 新型 RiPP 改性酶

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-09 DOI: 10.1016/j.cbpa.2024.102463

Daniel Richter, Jörn Piel

引用次数: 0

Peroxynitrite: a multifaceted oxidizing and nitrating metabolite 过亚硝酸盐：一种多方面的氧化和硝化代谢物

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-08 DOI: 10.1016/j.cbpa.2024.102459

Carolina Prolo , Lucía Piacenza , Rafael Radi

{"title":"Peroxynitrite: a multifaceted oxidizing and nitrating metabolite","authors":"Carolina Prolo , Lucía Piacenza , Rafael Radi","doi":"10.1016/j.cbpa.2024.102459","DOIUrl":"https://doi.org/10.1016/j.cbpa.2024.102459","url":null,"abstract":"<div><p>Peroxynitrite, a short-lived and reactive oxidant, emerges from the diffusion-controlled reaction between the superoxide radical and nitric oxide. Evidence shows that peroxynitrite is a critical mediator in physiological and pathological processes such as the immune response, inflammation, cancer, neurodegeneration, vascular dysfunction, and aging. The biochemistry of peroxynitrite is multifaceted, involving one- or two-electron oxidations and nitration reactions. This minireview highlights recent findings of peroxynitrite acting as a metabolic mediator in processes ranging from oxidative killing to redox signaling. Selected examples of nitrated proteins (<em>i.e.,</em> 3-nitrotyrosine) are surveyed to underscore the role of this post-translational modification on cell homeostasis. While accumulated evidence shows that large amounts of peroxynitrite participates of broad oxidation and nitration events in invading pathogens and host tissues, a closer look supports that low to moderate levels selectively trigger signal transduction cascades. Peroxynitrite probes and redox-based pharmacology are instrumental to further understand the biological actions of this reactive metabolite.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"80 ","pages":"Article 102459"},"PeriodicalIF":7.8,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in photoaffinity labeling strategies to capture Glycan–Protein interactions 捕捉糖蛋白相互作用的光亲和标记策略的最新进展

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-05-04 DOI: 10.1016/j.cbpa.2024.102456

Jonathan L. Babulic , Fabiola V. De León González , Chantelle J. Capicciotti

{"title":"Recent advances in photoaffinity labeling strategies to capture Glycan–Protein interactions","authors":"Jonathan L. Babulic , Fabiola V. De León González , Chantelle J. Capicciotti","doi":"10.1016/j.cbpa.2024.102456","DOIUrl":"https://doi.org/10.1016/j.cbpa.2024.102456","url":null,"abstract":"<div><p>Glycans decorate all cells and are critical mediators of cellular processes through recognition by glycan-binding proteins (GBPs). While targeting glycan–protein interactions has great therapeutic potential, these interactions are challenging to study as they are generally transient and exhibit low binding affinities. Glycan-based photo-crosslinkable probes have enabled covalent capture and identification of unknown GBP receptors and glycoconjugate ligands. Here, we review recent progress in photo-crosslinking approaches targeting glycan-mediated interactions. We discuss two prominent emerging strategies: 1) development of photo-crosslinkable oligosaccharide ligands to identify GBP receptors; and 2) cell-surface glyco-engineering to identify glycoconjugate ligands of GBPs. Overall, photoaffinity labeling affords valuable insights into complex glycan–protein networks and is poised to help elucidate the glycan–protein interactome, providing novel targets for therapeutic intervention.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"80 ","pages":"Article 102456"},"PeriodicalIF":7.8,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000322/pdfft?md5=bb20cf6e1a2e1e0523b2c9cc767b10c4&pid=1-s2.0-S1367593124000322-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140825487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functions and mechanisms of enzymes assembling lignans and norlignans 组装木酚素和诺尔木酚素的酶的功能和机制

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-04-30 DOI: 10.1016/j.cbpa.2024.102462

Richiro Ushimaru

引用次数: 0

Towards chemoenzymatic labeling strategies for profiling protein glycosylation 采用化学酶标记策略剖析蛋白质糖基化

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-04-27 DOI: 10.1016/j.cbpa.2024.102460

Yinping Tian , Shengzhou Ma , Liuqing Wen

引用次数: 0

Recent advances in minimal fluorescent probes for optical imaging 用于光学成像的最小荧光探针的最新进展

IF 7.8 2区生物学

Current Opinion in Chemical Biology Pub Date : 2024-04-25 DOI: 10.1016/j.cbpa.2024.102458

Fabio de Moliner , Ferran Nadal-Bufi , Marc Vendrell

{"title":"Recent advances in minimal fluorescent probes for optical imaging","authors":"Fabio de Moliner , Ferran Nadal-Bufi , Marc Vendrell","doi":"10.1016/j.cbpa.2024.102458","DOIUrl":"https://doi.org/10.1016/j.cbpa.2024.102458","url":null,"abstract":"<div><p>Fluorescent probes have revolutionized biological imaging by enabling the real-time visualization of cellular processes under physiological conditions. However, their size and potential perturbative nature can pose challenges in retaining the integrity of biological functions. This manuscript highlights recent advancements in the development of small fluorescent probes for optical imaging studies. Single benzene-based fluorophores offer versatility with minimal disruption, exhibiting diverse properties like aggregation-induced emission and pH responsiveness. Fluorescent nucleobases enable precise labeling of nucleic acids without compromising function, offering high sensitivity and compatibility with biochemistry studies. Bright yet small fluorescent amino acids provide an interesting alternative to bulky fusion proteins, facilitating non-invasive imaging of cellular events with high precision. These miniaturized fluorophores promise enhanced capabilities for studying biological systems in a non-invasive manner, fostering further innovations in molecular imaging.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"80 ","pages":"Article 102458"},"PeriodicalIF":7.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000346/pdfft?md5=ba2c27d3c9b85e9d0076f896b3a16938&pid=1-s2.0-S1367593124000346-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0