4 + 2] 环酶的发现与进化。

IF 6.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jiawang Liu, Youcai Hu
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引用次数: 0

摘要

[4+2]环化酶是大自然赋予微生物和植物的强效生物催化剂,为它们提供了利用和实施[4+2]环加成反应构建环己烯核心以合成有价值分子的有力工具。在过去两年中,有 11 种新的酶加入了这个周环酶俱乐部,并接受了广泛的研究。在本综述中,我们从催化机理和立体选择性的角度全面概述了[4 + 2]环化酶的最新进展。我们特别关注从酶共晶体结构、辅助因子以及糖基化效应中获得的见解。在了解天然[4 + 2]环化酶的机制方面取得的进展为模仿进化过程和设计人工酶以开发有价值的实用生物催化剂提供了可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Discovery and evolution of [4 + 2] cyclases

Discovery and evolution of [4 + 2] cyclases

[4 + 2] Cyclases are potent biocatalysts that have been bestowed upon microorganisms and plants by nature, equipping them with the powerful tools to utilize and implement the [4 + 2] cycloaddition reaction for constructing the cyclohexene core in synthesizing valuable molecules. Over the past two years, eleven new enzymes have joined this pericyclase club and undergone extensive investigation. In this review, we present a comprehensive overview of recent advancements in characterizing [4 + 2] cyclases with regard to their catalytic mechanism and stereoselectivity. We particularly focus on insights gained from enzyme co–crystal structures, cofactors, as well as the effects of glycosylation. Advancements in understanding the mechanisms of natural [4 + 2] cyclases offer the potential to mimic evolutionary processes and engineer artificial enzymes for the development of valuable and practical biocatalysts.

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来源期刊
Current Opinion in Chemical Biology
Current Opinion in Chemical Biology 生物-生化与分子生物学
CiteScore
13.30
自引率
1.30%
发文量
113
审稿时长
74 days
期刊介绍: COCHBI (Current Opinion in Chemical Biology) is a systematic review journal designed to offer specialists a unique and educational platform. Its goal is to help professionals stay informed about the growing volume of information in the field of Chemical Biology through systematic reviews.
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