发布求助
文献互助 智能选刊 最新文献

Current Opinion in Chemical Biology最新文献

筛选
英文 中文
Recent progresses in the cyclization and oxidation of polyketide biosynthesis 多酮生物合成的环化和氧化的最新进展。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-08-01 DOI: 10.1016/j.cbpa.2024.102507
Bo Zhang, Hui Ming Ge
{"title":"Recent progresses in the cyclization and oxidation of polyketide biosynthesis","authors":"Bo Zhang,&nbsp;Hui Ming Ge","doi":"10.1016/j.cbpa.2024.102507","DOIUrl":"10.1016/j.cbpa.2024.102507","url":null,"abstract":"<div><p>Polyketides represent an important class of natural products, renowned for their intricate structures and diverse biological activities. In contrast to common fatty acids, polyketides possess relatively more rigid carbon skeletons, more complex ring systems, and chiral centers. These structural features are primarily achieved through distinctive enzymatic cyclizations and oxidations as tailoring steps. In this opinion, we discuss the recent progress in deciphering the mechanisms of cyclization and oxidation within polyketide biosynthesis. By shedding light on these enzymatic processes, this article seeks to motivate the community to unravel the remaining mysteries surrounding cyclase and oxidase functionalities and to explore novel polyketide natural products through genome mining.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102507"},"PeriodicalIF":6.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Strategies and mechanisms for endosomal escape of therapeutic nucleic acids 治疗性核酸的内体逸出策略和机制。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-08-01 DOI: 10.1016/j.cbpa.2024.102506
Melina Grau , Ernst Wagner
{"title":"Strategies and mechanisms for endosomal escape of therapeutic nucleic acids","authors":"Melina Grau ,&nbsp;Ernst Wagner","doi":"10.1016/j.cbpa.2024.102506","DOIUrl":"10.1016/j.cbpa.2024.102506","url":null,"abstract":"<div><p>Despite impressive recent establishment of therapeutic nucleic acids as drugs and vaccines, their broader medical use is impaired by modest performance in intracellular delivery. Inefficient endosomal escape presents a major limitation responsible for inadequate cytosolic cargo release. Depending on the carrier, this endosomal barrier can strongly limit or even abolish nucleic acid delivery. Different recent endosomal escape strategies and their hypothesized mechanisms are reviewed.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102506"},"PeriodicalIF":6.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000826/pdfft?md5=2b89c2b69ecf385ac782b8ed8112060e&pid=1-s2.0-S1367593124000826-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery and evolution of [4 + 2] cyclases 4 + 2] 环酶的发现与进化。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-08-01 DOI: 10.1016/j.cbpa.2024.102504
Jiawang Liu, Youcai Hu
{"title":"Discovery and evolution of [4 + 2] cyclases","authors":"Jiawang Liu,&nbsp;Youcai Hu","doi":"10.1016/j.cbpa.2024.102504","DOIUrl":"10.1016/j.cbpa.2024.102504","url":null,"abstract":"<div><p>[4 + 2] Cyclases are potent biocatalysts that have been bestowed upon microorganisms and plants by nature, equipping them with the powerful tools to utilize and implement the [4 + 2] cycloaddition reaction for constructing the cyclohexene core in synthesizing valuable molecules. Over the past two years, eleven new enzymes have joined this pericyclase club and undergone extensive investigation. In this review, we present a comprehensive overview of recent advancements in characterizing [4 + 2] cyclases with regard to their catalytic mechanism and stereoselectivity. We particularly focus on insights gained from enzyme co–crystal structures, cofactors, as well as the effects of glycosylation. Advancements in understanding the mechanisms of natural [4 + 2] cyclases offer the potential to mimic evolutionary processes and engineer artificial enzymes for the development of valuable and practical biocatalysts.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102504"},"PeriodicalIF":6.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of artificial backbone connectivity in the development of metalloenzyme mimics 应用人工骨架连接技术开发金属酶模拟物。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-08-01 DOI: 10.1016/j.cbpa.2024.102509
Jacob A. Wolfe, W. Seth Horne
{"title":"Application of artificial backbone connectivity in the development of metalloenzyme mimics","authors":"Jacob A. Wolfe,&nbsp;W. Seth Horne","doi":"10.1016/j.cbpa.2024.102509","DOIUrl":"10.1016/j.cbpa.2024.102509","url":null,"abstract":"<div><p>Metal-dependent enzymes are abundant and vital catalytic agents in nature. The functional versatility of metalloenzymes has made them common targets for improvement by protein engineering as well as mimicry by <em>de novo</em> designed sequences. In both strategies, the incorporation of non-canonical cofactors and/or non-canonical side chains has proved a useful tool. Less explored—but similarly powerful—is the utilization of non-canonical covalent modifications to the polypeptide backbone itself. Such efforts can entail either introduction of limited artificial monomers in natural chains to produce heterogeneous backbones or construction of completely abiotic oligomers that adopt defined folds. Herein, we review recent research applying artificial protein-like backbones in the construction of metalloenzyme mimics, highlighting progress as well as open questions in this emerging field.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102509"},"PeriodicalIF":6.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000851/pdfft?md5=7682c3330f9c6aad812ea489aee31afc&pid=1-s2.0-S1367593124000851-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advances in the design and optimization of artificial metalloenzymes 人工金属酶设计和优化的最新进展。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-08-01 DOI: 10.1016/j.cbpa.2024.102508
Iori Morita, Thomas R. Ward
{"title":"Recent advances in the design and optimization of artificial metalloenzymes","authors":"Iori Morita,&nbsp;Thomas R. Ward","doi":"10.1016/j.cbpa.2024.102508","DOIUrl":"10.1016/j.cbpa.2024.102508","url":null,"abstract":"<div><p>Embedding a catalytically competent transition metal into a protein scaffold affords an artificial metalloenzyme (ArM). Such hybrid catalysts display features that are reminiscent of both homogeneous and enzymatic catalysts. Pioneered by Whitesides and Kaiser in the late 1970s, this field of ArMs has expanded over the past two decades, marked by ever-increasing diversity in reaction types, cofactors, and protein scaffolds. Recent noteworthy developments include i) the use of earth-abundant metal cofactors, ii) concurrent cascade reactions, iii) synergistic catalysis, and iv) <em>in vivo</em> catalysis. Thanks to significant progress in computational protein design, ArMs based on <em>de novo</em>–designed proteins and tailored chimeric proteins promise a bright future for this exciting field.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102508"},"PeriodicalIF":6.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glutathione dynamics in subcellular compartments and implications for drug development 亚细胞区室中的谷胱甘肽动态及其对药物开发的影响。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-08-01 DOI: 10.1016/j.cbpa.2024.102505
Hanfeng Lin , Lingfei Wang , Xiqian Jiang , Jin Wang
{"title":"Glutathione dynamics in subcellular compartments and implications for drug development","authors":"Hanfeng Lin ,&nbsp;Lingfei Wang ,&nbsp;Xiqian Jiang ,&nbsp;Jin Wang","doi":"10.1016/j.cbpa.2024.102505","DOIUrl":"10.1016/j.cbpa.2024.102505","url":null,"abstract":"<div><p>Glutathione (GSH) is a pivotal tripeptide antioxidant essential for maintaining cellular redox homeostasis and regulating diverse cellular processes. Subcellular compartmentalization of GSH underscores its multifaceted roles across various organelles including the cytosol, mitochondria, endoplasmic reticulum, and nucleus, each exhibiting distinct regulatory mechanisms. Perturbations in GSH dynamics contribute to pathophysiological conditions, emphasizing the clinical significance of understanding its intricate regulation. This review consolidates current knowledge on subcellular GSH dynamics, highlighting its implications in drug development, particularly in covalent drug design and antitumor strategies targeting intracellular GSH levels. Challenges and future directions in deciphering subcellular GSH dynamics are discussed, advocating for innovative methodologies to advance our comprehension and facilitate the development of precise therapeutic interventions based on GSH modulation.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102505"},"PeriodicalIF":6.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MicroRNA:Siglec crosstalk in cancer progression 微 RNA：Siglec 在癌症进展中的相互影响
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-07-18 DOI: 10.1016/j.cbpa.2024.102502
D. Mustafov , M.S. Ahmad , A. Serrano , M. Braoudaki , S.S. Siddiqui
{"title":"MicroRNA:Siglec crosstalk in cancer progression","authors":"D. Mustafov ,&nbsp;M.S. Ahmad ,&nbsp;A. Serrano ,&nbsp;M. Braoudaki ,&nbsp;S.S. Siddiqui","doi":"10.1016/j.cbpa.2024.102502","DOIUrl":"10.1016/j.cbpa.2024.102502","url":null,"abstract":"<div><p>Aberrant Siglec expression in the tumour microenvironment has been implicated in tumour malignancies and can impact tumour behaviour and patient survival. Further to this, engagement with sialoglycans induces masked antigen recognition and promotes immune evasion, highlighting deregulated immune function. This necessitates the elucidation of their expression profiles in tumour progression. MicroRNAs (miRNAs) mediated targeting represents a novel approach to further elucidate Siglec potential and clinical relevance. Although miRNA activity in Siglec expression remains limited, we highlight current literature detailing miRNA:Siglec interactions within the tumour landscape and provide insights for possible diagnostic and therapeutic strategies in targeting the Siglec/sialic acid axis.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102502"},"PeriodicalIF":6.9,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1367593124000784/pdfft?md5=fa5f5ed0a0c37a6bae4a3703795bb511&pid=1-s2.0-S1367593124000784-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advances in methods for quantifying the cell penetration of macromolecules 大分子细胞渗透量化方法的最新进展
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-07-17 DOI: 10.1016/j.cbpa.2024.102501
Nefeli Batistatou, Joshua A. Kritzer
{"title":"Recent advances in methods for quantifying the cell penetration of macromolecules","authors":"Nefeli Batistatou,&nbsp;Joshua A. Kritzer","doi":"10.1016/j.cbpa.2024.102501","DOIUrl":"10.1016/j.cbpa.2024.102501","url":null,"abstract":"<div><p>As the landscape of macromolecule therapeutics advances, drug developers are continuing to aim at intracellular targets. To activate, inhibit, or degrade these targets, the macromolecule must be delivered efficiently to intracellular compartments. Quite often, there is a discrepancy between binding affinity in biochemical assays and activity in cell-based assays. Identifying the bottleneck for cell-based activity requires robust assays that quantify total cellular uptake and/or cytosolic delivery. Recognizing this need, chemical biologists have designed a plethora of assays to make this measurement, each with distinct advantages and disadvantages. In this review, we describe the latest and most promising developments in the last 3 to 4 years.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102501"},"PeriodicalIF":6.9,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rational design of lipid nanoparticles: overcoming physiological barriers for selective intracellular mRNA delivery 合理设计脂质纳米颗粒：克服生理障碍，选择性地在细胞内输送 mRNA。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-07-13 DOI: 10.1016/j.cbpa.2024.102499
Yu Zhao , Zeyu Morgan Wang , Donghui Song , Mengting Chen, Qiaobing Xu
{"title":"Rational design of lipid nanoparticles: overcoming physiological barriers for selective intracellular mRNA delivery","authors":"Yu Zhao ,&nbsp;Zeyu Morgan Wang ,&nbsp;Donghui Song ,&nbsp;Mengting Chen,&nbsp;Qiaobing Xu","doi":"10.1016/j.cbpa.2024.102499","DOIUrl":"10.1016/j.cbpa.2024.102499","url":null,"abstract":"<div><p>This review introduces the typical delivery process of messenger RNA (mRNA) nanomedicines and concludes that the delivery involves a at least four-step <strong>SCER</strong> cascade and that high efficiency at every step is critical to guarantee high overall therapeutic outcomes. This <strong>SCER</strong> cascade process includes selective organ-targeting delivery, cellular uptake, endosomal escape, and cytosolic mRNA release. Lipid nanoparticles (LNPs) have emerged as a state-of-the-art vehicle for <em>in vivo</em> mRNA delivery. The review emphasizes the importance of LNPs in achieving selective, efficient, and safe mRNA delivery. The discussion then extends to the technical and clinical considerations of LNPs, detailing the roles of individual components in the SCER cascade process, especially ionizable lipids and helper phospholipids. The review aims to provide an updated overview of LNP-based mRNA delivery, outlining recent innovations and addressing challenges while exploring future developments for clinical translation over the next decade.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102499"},"PeriodicalIF":6.9,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141597988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial glycoengineering: Cell-based and cell-free routes for producing biopharmaceuticals with customized glycosylation 细菌糖工程：以细胞为基础和无细胞途径生产定制糖基化生物制药。
IF 6.9 2区 生物学
Current Opinion in Chemical Biology Pub Date : 2024-07-10 DOI: 10.1016/j.cbpa.2024.102500
Jaymee A. Palma , Mehman I. Bunyatov , Sophia W. Hulbert , Michael C. Jewett , Matthew P. DeLisa
{"title":"Bacterial glycoengineering: Cell-based and cell-free routes for producing biopharmaceuticals with customized glycosylation","authors":"Jaymee A. Palma ,&nbsp;Mehman I. Bunyatov ,&nbsp;Sophia W. Hulbert ,&nbsp;Michael C. Jewett ,&nbsp;Matthew P. DeLisa","doi":"10.1016/j.cbpa.2024.102500","DOIUrl":"10.1016/j.cbpa.2024.102500","url":null,"abstract":"<div><p>Glycosylation plays a pivotal role in tuning the folding and function of proteins. Because most human therapeutic proteins are glycosylated, understanding and controlling glycosylation is important for the design, optimization, and manufacture of biopharmaceuticals. Unfortunately, natural eukaryotic glycosylation pathways are complex and often produce heterogeneous glycan patterns, making the production of glycoproteins with chemically precise and homogeneous glycan structures difficult. To overcome these limitations, bacterial glycoengineering has emerged as a simple, cost-effective, and scalable approach to produce designer glycoprotein therapeutics and vaccines in which the glycan structures are engineered to reduce heterogeneity and improve biological and biophysical attributes of the protein. Here, we discuss recent advances in bacterial cell-based and cell-free glycoengineering that have enabled the production of biopharmaceutical glycoproteins with customized glycan structures.</p></div>","PeriodicalId":291,"journal":{"name":"Current Opinion in Chemical Biology","volume":"81 ","pages":"Article 102500"},"PeriodicalIF":6.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信