Recent advances in the expanding genetic code

IF 6.9 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Michael L. Pigula, Peter G. Schultz
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引用次数: 0

Abstract

For over a billion years, the central dogma of biology has been limited largely to 20 canonical amino acids with relatively simple functionalities. The ability to rationally add new building blocks to the genetic code has enabled the site-specific incorporation of hundreds of noncanonical amino acids (ncAAs) with novel properties into proteins in living organisms. Recent technological advances have enabled high level mammalian expression of proteins containing ncAAs, the use of unique codons to direct ncAA incorporation, extension of this methodology to a range of eukaryotic organisms, and the ability to encode building blocks beyond α-amino acids. These ncAAs have been used to study and control proteins in their native cellular context and to engineer enzymes and biotherapeutics with improved or novel properties. Herein we discuss recent developments in the field and potential future research directions.
扩展遗传密码的最新进展。
十多亿年来,生物学的核心教条一直局限于 20 个功能相对简单的典型氨基酸。在遗传密码中合理添加新构建模块的能力,使数百种具有新特性的非规范氨基酸(ncAAs)能够特异性地加入生物体的蛋白质中。最近的技术进步实现了含有 ncAAs 的蛋白质在哺乳动物体内的高水平表达,使用独特的密码子指导 ncAAs 的结合,将这种方法推广到一系列真核生物体,并能够编码 α 氨基酸以外的构建模块。这些 ncAAs 已被用于研究和控制原生细胞环境中的蛋白质,以及设计具有改进或新特性的酶和生物治疗药物。在此,我们将讨论该领域的最新进展以及未来潜在的研究方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Opinion in Chemical Biology
Current Opinion in Chemical Biology 生物-生化与分子生物学
CiteScore
13.30
自引率
1.30%
发文量
113
审稿时长
74 days
期刊介绍: COCHBI (Current Opinion in Chemical Biology) is a systematic review journal designed to offer specialists a unique and educational platform. Its goal is to help professionals stay informed about the growing volume of information in the field of Chemical Biology through systematic reviews.
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