IF 19.6 1区化学

Chem Pub Date : 2025-09-11 DOI: 10.1016/j.chempr.2025.102745

David A. Leigh

引用次数: 0

Toward synthetic life—Emergence, growth, creation of offspring, decay, and rescue of fuel-dependent synthetic cells 走向合成生命——依赖燃料的合成细胞的出现、生长、后代的创造、衰变和拯救

IF 19.6 1区化学

Chem Pub Date : 2025-09-11 DOI: 10.1016/j.chempr.2025.102578

Monika Wenisch , Yinqing Li , Marius G. Braun , Lukas Eylert , Fabian Späth , Simone M. Poprawa , Bernhard Rieger , Christopher V. Synatschke , Henrike Niederholtmeyer , Job Boekhoven

{"title":"Toward synthetic life—Emergence, growth, creation of offspring, decay, and rescue of fuel-dependent synthetic cells","authors":"Monika Wenisch , Yinqing Li , Marius G. Braun , Lukas Eylert , Fabian Späth , Simone M. Poprawa , Bernhard Rieger , Christopher V. Synatschke , Henrike Niederholtmeyer , Job Boekhoven","doi":"10.1016/j.chempr.2025.102578","DOIUrl":"10.1016/j.chempr.2025.102578","url":null,"abstract":"<div><div>The <em>de novo</em> synthesis of life from non-living matter represents a bold scientific challenge, advancing our understanding of life’s minimal requirements and offering revolutionary applications in biotechnology. We explore fuel-dependent synthetic cells based on complex coacervate droplets, which lack membranes and readily take up reactants. Given their fuel-dependent nature, these droplets emerge and grow when fuel is abundant but dissolve under starvation conditions, mimicking the non-equilibrium nature of life. However, their ability to produce offspring—a key requirement for life—has remained elusive. Moreover, their rescue in repetitive fueling-starvation experiments has not been demonstrated. Our work elucidates a mechanism of producing offspring by synthetic cells driven by solid-like speckles in droplets liberated as offspring. By fine-tuning parameters, we control offspring number and survival. Finally, refueling sustains second-generation synthetic cells. This system provides a platform for coupling offspring production with self-replicating molecules, paving the way for synthetic cells capable of Darwinian evolution.</div></div>","PeriodicalId":268,"journal":{"name":"Chem","volume":"11 9","pages":"Article 102578"},"PeriodicalIF":19.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and assembly strategy of electroactive biomaterials and systems for soft tissue engineering applications 用于软组织工程的电活性生物材料和系统的合成与装配策略

IF 19.6 1区化学

Chem Pub Date : 2025-09-11 DOI: 10.1016/j.chempr.2025.102596

Dong Yang , Yi Hu , Shuai Liu , Wenhui Yang , Xi Ren , Yanbing Li , Feng Xu , Min Gong , Yunlong Zhao , Xiaozhong Qiu , Honghao Hou

{"title":"Synthesis and assembly strategy of electroactive biomaterials and systems for soft tissue engineering applications","authors":"Dong Yang , Yi Hu , Shuai Liu , Wenhui Yang , Xi Ren , Yanbing Li , Feng Xu , Min Gong , Yunlong Zhao , Xiaozhong Qiu , Honghao Hou","doi":"10.1016/j.chempr.2025.102596","DOIUrl":"10.1016/j.chempr.2025.102596","url":null,"abstract":"<div><div>Soft tissue injuries are a common issue affecting human health, and the extent and duration of injuries often constrain the healing process. Electroactive biomaterials have become a key medium for tissue regeneration by modulating cellular behavior, but a systematic evaluation of their potential and challenges is still limited. This review comprehensively explores electroactive biomaterials, focusing on their chemical synthesis, assembly processes, application potential, challenges faced, and future directions. First, this work elaborates on the unique and innovative features of electroactive biomaterials’ chemical synthesis and preparation techniques. Subsequently, the principles of electroactive devices and their interactions with biological tissues are described to optimize tissue repair. Finally, the application prospects of electrical stimulation technology in soft tissue engineering are explored, emphasizing its role in cell and tissue regeneration and its clinical potential. In conclusion, this review provides theoretical and practical insights into the development and application of electroactive biomaterials.</div></div>","PeriodicalId":268,"journal":{"name":"Chem","volume":"11 9","pages":"Article 102596"},"PeriodicalIF":19.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Weaving in polyhedra 多面体编织

IF 19.6 1区化学

Chem Pub Date : 2025-09-11 DOI: 10.1016/j.chempr.2025.102748

Raorao Yang , Liang Zhang

引用次数: 0

Cancer nanomedicine: Concepts, promises, and challenges 癌症纳米医学：概念、前景和挑战

IF 19.6 1区化学

Chem Pub Date : 2025-09-11 DOI: 10.1016/j.chempr.2025.102706

Chunlei Zhu , Eduard Preis , Udo Bakowsky , Younan Xia

引用次数: 0

N-Heterocyclic carbene-derived second-period main-group radicals: Synthesis, fundamentals, and applications n-杂环碳衍生的二周期主基自由基：合成、基本原理及应用

IF 23.5 1区化学

Chem Pub Date : 2025-09-04 DOI: 10.1016/j.chempr.2025.102714

Solhye Choe, Chaerin Lim, Sunghoon Kang, Eunsung Lee

引用次数: 0

Measuring kcat/KM values for over 200,000 enzymatic substrates with mRNA display 用mRNA显示测量超过200,000种酶促底物的kcat/KM值

IF 23.5 1区化学

Chem Pub Date : 2025-09-03 DOI: 10.1016/j.chempr.2025.102737

Alexander A. Vinogradov, Hiroaki Suga

{"title":"Measuring kcat/KM values for over 200,000 enzymatic substrates with mRNA display","authors":"Alexander A. Vinogradov, Hiroaki Suga","doi":"10.1016/j.chempr.2025.102737","DOIUrl":"https://doi.org/10.1016/j.chempr.2025.102737","url":null,"abstract":"Recent advances in enzymology are enabled by the methods for high-throughput kinetic measurements. Common instrumentation-based techniques can examine thousands of enzymatic reactions in parallel, but scaling the throughput further can be challenging. Here, we establish DOMEK (mRNA-display-based one-shot measurement of enzymatic kinetics), an integrated experimental and computational pipeline for ultra-high-throughput kinetic measurements using mRNA display-derived next-generation sequencing data. The method can accurately determine k<sub>cat</sub>/K<sub>M</sub> specificity constants of post-translational modification enzyme substrates. We benchmark the platform by measuring k<sub>cat</sub>/K<sub>M</sub> values for ∼2.86 × 10<sup>5</sup> peptide substrates of a dehydroalanine reductase and leverage the resulting data to build interpretable models of the substrate fitness landscape. The resulting model accurately decomposes reaction activation energies of a peptide substrate into energetic contributions of individual amino acids to reveal microscopic and macroscopic aspects of the enzyme catalysis. Our results establish a generalizable, enzyme-agnostic framework for scaling kinetic measurements to millions of reactions.","PeriodicalId":268,"journal":{"name":"Chem","volume":"52 1","pages":""},"PeriodicalIF":23.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144930565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modular synthesis of fluoroalkyl-substituted cyclopropenes with fluoroalkylacylsilanes as carbynyl cation equivalents 氟烷基取代环丙烯与氟烷基丙烯基硅烷作为羰基阳离子等价物的模块化合成

IF 23.5 1区化学

Chem Pub Date : 2025-09-03 DOI: 10.1016/j.chempr.2025.102721

Gang Zhou, Yuan Yao, Xiaoqian He, Weilu Zhang, Shanshan Liu, Xiao Shen

引用次数: 0

Construction of chiral nitrogen stereocenters via enantioselective C–H activation 通过对映选择性碳氢活化构建手性氮立体中心

IF 23.5 1区化学

Chem Pub Date : 2025-09-03 DOI: 10.1016/j.chempr.2025.102730

Zechen Wu, Dongsheng Yi, Junhao Tang, Fanyi Meng, Haining Zhu, Kevin Wu, Kaining Duanmu, Jin-Quan Yu, Yanghui Zhang

{"title":"Construction of chiral nitrogen stereocenters via enantioselective C–H activation","authors":"Zechen Wu, Dongsheng Yi, Junhao Tang, Fanyi Meng, Haining Zhu, Kevin Wu, Kaining Duanmu, Jin-Quan Yu, Yanghui Zhang","doi":"10.1016/j.chempr.2025.102730","DOIUrl":"https://doi.org/10.1016/j.chempr.2025.102730","url":null,"abstract":"Enantioselective C–H activation has recently emerged as a versatile method for selectively constructing point, axial, and planar chirality. Out of the main strategies in asymmetric catalysis, desymmetrization, enantioselection, and classical kinetic resolutions have been demonstrated in C–H activation reactions. To the best of our knowledge, dynamic kinetic resolution (DKR) via enantioselective C(sp<sup>3</sup>)-H activation to set point chirality remains unrealized. While the rapid inversion of nitrogen stereocenters typically presents a challenge to their enantioselective synthesis, this phenomenon provides a unique opportunity to selectively access nitrogen stereocenters via a DKR given the appropriate system. Here, we report the DKR of nitrogen stereocenters via a Pd-catalyzed enantioselective C–H olefination yielding enantioenriched tribenzo[<em>b</em>,<em>d</em>,<em>f</em>]azepines and dibenzo[<em>b</em>,<em>f</em>]azepines, thus demonstrating the feasibility of constructing enantioenriched nitrogen stereocenters by harnessing nitrogen inversion. The chiral tribenzo[<em>b</em>,<em>d</em>,<em>f</em>]azepine products exhibit high circularly polarized luminescence (CPL) performance, representing a novel scaffold for exploration in this area.","PeriodicalId":268,"journal":{"name":"Chem","volume":"52 1","pages":""},"PeriodicalIF":23.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144930566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unexpected diastereoselective chemistry on a 2D protein surface 二维蛋白质表面意想不到的非对映选择性化学反应

IF 23.5 1区化学

Chem Pub Date : 2025-09-02 DOI: 10.1016/j.chempr.2025.102717

Zhong Hui Lim, Zonghua Bo, Emily Armstrong, Hagan Bayley, Yujia Qing

{"title":"Unexpected diastereoselective chemistry on a 2D protein surface","authors":"Zhong Hui Lim, Zonghua Bo, Emily Armstrong, Hagan Bayley, Yujia Qing","doi":"10.1016/j.chempr.2025.102717","DOIUrl":"https://doi.org/10.1016/j.chempr.2025.102717","url":null,"abstract":"Enantioselective enzymes feature structured catalytic sites positioned within binding pockets. In contrast, widely occurring flat protein surfaces, which have been suggested to have emerged early in ancestral protein evolution as solvent-exposed β sheets, appear unlikely to catalyze asymmetric reactions because they lack the necessary scaffold for interacting with substrates in three dimensions. At the near-flat, water-accessible surface within the α-hemolysin transmembrane pore, we now demonstrate remarkable diastereoselectivity in hemithioacetal formation between a cysteine side chain and a series of aldehyde substrates. After protein surface remodeling by mutagenesis, diastereomeric ratios of up to 95:5 (kinetic control) and 98:2 (thermodynamic control) were achieved with a range of aromatic aldehydes. Molecular dynamics simulations confirmed asymmetric interactions between adducts and nearby side chains in a two-dimensional plane. Our findings indicate that flat protein surfaces can scaffold stereoselective chemistry, thereby expanding the designable protein space for catalyst engineering and providing insight into the origin of selective enzymes.","PeriodicalId":268,"journal":{"name":"Chem","volume":"14 1","pages":""},"PeriodicalIF":23.5,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144928638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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