Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences最新文献

{"title":"[Role of myeloid cell abnormalities in the pathogenesis of aplastic anemia:a review of research advances].","authors":"Mingyi Wang, Haichen Lin, Xuejun Zhu","doi":"10.3724/zdxbyxb-2025-0881","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2025-0881","url":null,"abstract":"<p><p>Aplastic anemia is a disorder characterized by bone marrow failure and peripheral pancytopenia. T cell-mediated injury to hematopoietic stem and progenitor cells (HSPCs) is recognized as a key immunological mechanism underlying its pathogenesis. Recent evidence indicates that myeloid cell abnormalities also contribute to the bone marrow immunopathology of aplastic anemia. Myeloid populations-including dendritic cells, mononuclear phagocytes, myeloid-derived suppressor cells, and mast cells-may disrupt the local bone marrow immune and hematopoietic microenvironment through their involvement in antigen presentation, immunosuppression, inflammation resolution, efferocytosis, and stromal support. Such abnormalities may not only sustain aberrant T cell responses and impair hematopoietic support, but also correlate with disease relapse and clonal evolution. This review summarizes current understanding of the role of myeloid cell abnormalities in the pathogenesis of aplastic anemia, aiming to provide a reference for further elucidation of its immune mechanisms and disease progression.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148901090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genetic and functional characterization of a novel <i>KIT</i> splicing variant in a Chinese three-generation pedigree with piebaldism].","authors":"Kai Yan, Liya Wang, Jiexue Pan, Hefeng Huang, Minyue Dong","doi":"10.3724/zdxbyxb-2026-0448","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2026-0448","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the genetic etiology of a three-generation pedigree affected with piebaldism.</p><p><strong>Methods: </strong>Next-generation sequencing and Sanger sequencing were employed to detect and verify gene variants. Bioinformatics tools were used to predict the effects of candidate variants on splicing and protein function. RT-PCR and Sanger sequencing were further performed to validate the impact of the variant on RNA splicing, and homology modeling was applied to predict its effect on the three-dimensional structure of the KIT protein. The pathogenicity of the variant was then classified according to the guidelines of the American College of Medical Genetics and Genomics (ACMG) and the UK Association for Clinical Genomic Science (ACGS).</p><p><strong>Results: </strong>A heterozygous insertion variant near the splice site, c.1990+8_1990+9insTGCACCATTGGAGGTAAA, was identified in the <i>KIT</i> gene in the proband and was found to co-segregate with the phenotype within the family. RT-PCR and cDNA sequencing revealed that this variant led to aberrant splicing during transcription, resulting in a 21 bp in-frame insertion in the mRNA, which encodes an extra 7 amino acids within the tyrosine kinase domain and may thus affect protein function. In silico predictions, together with the experimental findings, supported classification of this variant as likely pathogenic according to relevant variant interpretation guidelines.</p><p><strong>Conclusions: </strong>The heterozygous splice-site insertion variant KIT:c.1990+8_1990+9insTGCACCATTGGAGGTAAA is the genetic cause of piebaldism in this pedigree.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148901046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of digital-driven cardiac rehabilitation on clinical outcomes in patients undergoing transcatheter aortic valve replacement].","authors":"Danyan Yang, Feiyu Wu, Qifeng Zhu, Yang Zhan, Yue Mao, Xianbao Liu","doi":"10.3724/zdxbyxb-2026-0179","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2026-0179","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effects of digital-driven cardiac rehabilitation on clinical outcomes in patients undergoing transcatheter aortic valve replacement (TAVR).</p><p><strong>Methods: </strong>We retrospectively analyzed data from 1,012 patients who underwent TAVR at the Second Affiliated Hospital, Zhejiang University School of Medicine, between March 2013 and January 2023. Patients receiving conventional rehabilitation were assigned to the control group, whereas those receiving a digital-driven cardiac rehabilitation program, comprising preoperative screening and functional preparation, intraoperative eligibility assessment, postoperative stepwise rehabilitation management, and intelligent home-based rehabilitation, were assigned to the intervention group. Propensity score matching was performed to adjust for baseline confounding. Postoperative intensive care unit (ICU) stay and hospital stay, the incidence of cardiovascular and cerebrovascular adverse events at 30 days, 1 year, and 2 years after TAVR, and functional outcomes, including the 5-meter walk test, 6-minute walk test, and Katz Index, were compared between the two groups.</p><p><strong>Results: </strong>After propensity score matching, 251 patients were included in each group. Compared with the control group, the intervention group had significantly shorter postoperative ICU stays [0 (0, 0) h vs. 0 (0, 1) h, <i>P</i><0.01] and postoperative hospital stays [3 (1, 7) days vs. 8 (7, 11) days, <i>P</i><0.01]. The time required to complete the 5-meter walk test was significantly shorter in the intervention group than in the control group at 30 days, 1 year, and 2 years after TAVR (all <i>P</i><0.01). No apparent increase in the incidence of adverse events was observed in the intervention group at 30 days, 1 year, or 2 years after TAVR.</p><p><strong>Conclusions: </strong>The present findings suggest that digital-driven cardiac rehabilitation may shorten hospital stay and improve selected functional outcomes in patients undergoing TAVR without an observed increase in postoperative cardiovascular and cerebrovascular adverse events.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanisms of bone-brain axis involvement in concurrent nervous and skeletal system impairments associated with narcotic drug addiction: a review].","authors":"Yi Yang, Shuaixiong Liao, Tingran Zhang, Jiong Luo","doi":"10.3724/zdxbyxb-2026-0001","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2026-0001","url":null,"abstract":"<p><p>Narcotic drug addiction is characterized not only by compulsive drug use and persistent craving after withdrawal but also by systemic consequences of long-term exposure. These include alterations in neural circuits subserving reward, stress, and executive control, as well as skeletal impairments such as reduced bone mineral density and strength, disrupted bone turnover, muscle weakness, and elevated fracture risk. The bone-brain axis, which mediates bidirectional communication between the central nervous system and the skeleton via neural, endocrine, immune, metabolic, and bone-derived signaling, offers an integrative framework for understanding the concurrent neuro-skeletal impairments seen in addiction. Such impairments may not arise independently; rather, they may reflect cross-system pathology driven by interactions among neuroendocrine dysregulation, inflammatory and immune activation, oxidative stress, neurotransmitter disturbances, and feedback from bone-derived factors. Specifically, prolonged drug exposure can alter bone remodeling through the sympathetic nervous system, the hypothalamic-pituitary-adrenal (HPA) axis, and the hypothalamic-pituitary-gonadal (HPG) axis, while simultaneously disrupting the tissue microenvironment of both brain and bone via inflammatory/immune activation and redox imbalance. Abnormalities in monoamines-dopamine, serotonin, and norepinephrine-may further link addiction-related neurobehavioral changes to bone cell dysfunction. Meanwhile, bone-derived factors such as osteocalcin, sclerostin, lipocalin-2, and bone-derived extracellular vesicles may contribute to feedback regulation of brain function by skeletal tissue. This review summarizes the concurrent impairments of the nervous and skeletal systems associated with narcotic drug addiction, analyzes the potential pathways by which bone-brain axis dysregulation may mediate their interrelationship, and aims to provide a reference for elucidating the mechanisms of somatic comorbidity in addiction and for informing comprehensive intervention strategies.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on the role of claustrum in substance use disorders].","authors":"Manqing Wu, Wenhua Zhou, Min Zhao","doi":"10.3724/zdxbyxb-2025-0893","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2025-0893","url":null,"abstract":"<p><p>Substance use disorder (SUD) is characterized by the persistent prioritization of drug-related cues, which readily trigger intense craving and relapse even after prolonged abstinence. Although previous studies have primarily focused on the mesolimbic dopamine system and fronto-striatal circuits, the mechanisms underlying the aberrant integration of reward value, affective states, attentional allocation, and memory representations remain largely elusive. The claustrum-owing to its extensive bidirectional connectivity with both cortical and subcortical networks and its hypothesized role in salience processing-has increasingly been recognized as a key hub in SUD. Accumulating evidence suggests that the claustrum may contribute to drug-cue salience attribution and reward memory encoding, modulate prefrontal information processing and executive control via its reciprocal projections, and interact with limbic systems to sustain withdrawal-related negative affect, thereby influencing relapse vulnerability. Furthermore, emerging findings from psychedelic research indicate that claustrum-related networks are highly plastic and participate in salience reconfiguration. Collectively, the claustrum may act as a cross-network hub that links reward processing, cognitive control, and affective memory, thereby extending classical circuit models of addiction. This review systematically summarizes the structural connectivity, neuroimaging correlates, and functional mechanisms of the claustrum, and discusses its translational implications for relapse prediction and the development of novel intervention strategies.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Aberrant activation mechanisms of the NOTCH signaling pathway in T lymphoblastic lymphoma: mechanisms and targeted therapeutic implications].","authors":"Zhexin Li, Guzhengyue Zheng","doi":"10.3724/zdxbyxb-2026-0068","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2026-0068","url":null,"abstract":"<p><p>T-cell lymphoblastic lymphoma (T-LBL) is a highly aggressive non-Hodgkin lymphoma derived from precursor T cells, characterized by rapid clinical progression and a high relapse rate, with a particularly unfavorable prognosis in adult patients. Accumulating evidence indicates that the NOTCH signaling pathway plays a central oncogenic role in the initiation and progression of T-LBL. Its sustained aberrant activation is driven by multiple layers of mechanisms, including NOTCH1 gene mutations or fusions, synergistic amplification through multiple oncogenic signaling cascades, and dysregulated epigenetic control. Aberrantly activated NOTCH signaling promotes tumor cell proliferation, suppresses apoptosis, and contributes to chemotherapy resistance and disease relapse. This review focuses on the mechanisms underlying aberrant NOTCH activation in T-LBL and summarizes the principal therapeutic strategies targeting this pathway, along with their limitations, aiming to provide a framework for deeper under-standing of the molecular pathogenesis of T-LBL and for the development of more precise targeted therapies.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148892542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficacy and safety of injectable sodium hyaluronate gel in correcting moderate to severe nasolabial folds: a single-center, randomized, double-blind, controlled trial].","authors":"Jian Zhang, Chengzhi Dong, Yifeng Wang","doi":"10.3724/zdxbyxb-2026-0073","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2026-0073","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the 12-month clinical efficacy and safety of Dr. Korman® injectable cross-linked sodium hyaluronate gel (DKL) in correcting moderate to severe nasolabial folds.</p><p><strong>Methods: </strong>This was a single-center, prospective, randomized, double-blind, parallel-controlled trial. A total of 80 subjects with moderate to severe nasolabial folds (Wrinkle Severity Rating Scale [WSRS] score 3-4) were enrolled at Tongde Hospital of Zhejiang Province from October 2022 to June 2025 and assigned 1∶1 to the trial group (DKL) or the control group (Restylane® 2), with 40 cases each. Both groups received a layered injection strategy using a sharp needle for the periosteal layer and a blunt cannula for the subcutaneous fat layer. Subjects who failed to achieve a WSRS score reduction of ≥1 point at 1 month post-injection received a supplementary injection. Efficacy was evaluated using the WSRS and Global Aesthetic Improvement Scale (GAIS) at baseline and at 1, 3, 6, and 12 months after treatment. Local reactions and adverse events were recorded throughout the study. The primary efficacy endpoint was the bilateral WSRS score at 12 months post-injection. Equivalence was tested using two one-sided t-tests (TOST), and clinical relevance was assessed with Cohen's d effect size.</p><p><strong>Results: </strong>Baseline characteristics-including age, sex, nasolabial fold severity, and injection volume-were balanced between two groups (all <i>P</i> > 0.05). Both groups showed significant improvements in WSRS and GAIS scores at all follow-up time points compared with baseline (all <i>P</i> < 0.01), with no statistically significant intergroup differences at any time point (all <i>P</i> > 0.05; Cohen's d < 0.2). At 12 months, the between-group difference in WSRS score was ≤0.02 points (95% CI: -0.21 to 0.23), and both one-sided tests of the TOST yielded <i>P</i> < 0.05, confirming statistical equivalence. GAIS results indicated that efficacy in the trial group peaked at 1-3 months, with a mild decline in improvement at 6 months; scores at 12 months remained significantly better than baseline (all <i>P</i> < 0.01; Cohen's d > 0.5), demonstrating stable maintenance of clinical improvement for 12 months. Regarding safety, there were no significant differences between groups in the incidence of expected physiological local reactions (all <i>P</i> > 0.05). No serious adverse events occurred during the 12-month follow-up. The overall adverse event rates were 7.50% in the trial group and 10.00% in the control group, with no significant difference (<i>P</i> > 0.05); all events resolved spontaneously.</p><p><strong>Conclusions: </strong>DKL is equivalent to Restylane® 2 in 12-month efficacy and exhibits a favorable safety profile for correcting moderate to severe nasolabial folds, offering a new option for minimally invasive filling correction in clinical practice.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148841353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Angelica sinensis</i> promotes thymic cortical regeneration in mice with acute thymic involution induced by short-term rapamycin treatment].","authors":"Xie Xu, Yaoying Shu, Jianli Gao","doi":"10.3724/zdxbyxb-2025-0396","DOIUrl":"10.3724/zdxbyxb-2025-0396","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To investigate the effect of &lt;i&gt;Angelica sinensis&lt;/i&gt; on thymic cortical regeneration in rapamycin-treated mice and its underlying mechanisms.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Seventy-eight 6-8-week female BALB/c mice were randomly assigned by weight into six groups: blank control, model control, normal regeneration, and &lt;i&gt;Angelica sinensis&lt;/i&gt; low-dose, medium-dose, and high-dose groups (the blank control group &lt;i&gt;n&lt;/i&gt;=18, each of the other groups &lt;i&gt;n&lt;/i&gt;=12). The blank control group received a daily intraperitoneal injection of isotonic saline (0.1 mL/10 g), while the other groups were intraperitoneally administered rapamycin (1 mg·kg&lt;sup&gt;－&lt;/sup&gt;¹·d&lt;sup&gt;－&lt;/sup&gt;¹) for three consecutive days to establish acute thymic involution models. After modeling, the &lt;i&gt;Angelica sinensis&lt;/i&gt; groups received corresponding doses of &lt;i&gt;Angelica sinensis&lt;/i&gt; (1, 2, 4 g/kg per day), while the normal regeneration group received an equal volume of saline. Body weight and dorsal hair growth were recorded daily. Forelimb grip strength was measured 2 hours after the last administration. Thymic tissue structure and the spatial distribution of thymic epithelial cells (TECs) and thymocytes were assessed by hematoxylin-eosin (HE) and immunofluorescence stainings. Development and homeostasis of T-cell subsets in the thymus and peripheral blood were analyzed by flow cytometry and rapid Wright-Giemsa staining. T-cell receptor excision circles in genomic DNA from peripheral blood mononuclear cells were detected by quantitative PCR (qPCR). The mRNA expression levels of thymic function-related genes, inflammatory factors, and Wnt signaling pathway-related genes were measured by quantitative reverse transcription PCR (qRT-PCR). Potential targets and signaling pathways were screened by integrating network pharmacology prediction and molecular docking.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Rapamycin successfully induced acute thymic involution. The model control group showed a decrease in thymic index, weakened grip strength (both &lt;i&gt;P&lt;/i&gt;&lt;0.05), and no obvious hair regeneration. Histologically, the thymic cortical area was reduced, with a blurred corticomedullary junction, disrupted continuity of the cortical TEC cytoplasmic process network, early-stage blockade of thymocyte development, disturbed spatial distribution, and inhibited thymic output. Compared with the normal regeneration group, medium and high doses of &lt;i&gt;Angelica sinensis&lt;/i&gt; increased the cortical area in a dose-dependent manner, and significantly enhanced the density of cortical CK8&lt;sup&gt;+&lt;/sup&gt; TECs and restored the continuity of their cytoplasmic process network (all &lt;i&gt;P&lt;/i&gt;&lt;0.01), while upregulating the gene expression of &lt;i&gt;Foxn1&lt;/i&gt; and its downstream target gene &lt;i&gt;Dll4&lt;/i&gt; (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Regarding thymocyte development, high dose &lt;i&gt;Angelica sinensis&lt;/i&gt; increased the proportion of CD3&lt;sup&gt;+&lt;/sup&gt;TCRβ&lt;sup&gt;+&lt;/sup&gt; thymocytes (&lt;i&gt;P&lt;/i&gt;&lt;0.05), promoted the balanced differenti","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"734-750"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148206325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and validation of a comprehensive evaluation index system for preconception health behaviors in reproduc-tive-aged populations].","authors":"Ruyu Sun, Hanqian Wang, Xin Xu, Lu Li","doi":"10.3724/zdxbyxb-2025-0573","DOIUrl":"10.3724/zdxbyxb-2025-0573","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To develop and validate a comprehensive evaluation index system for preconception health behaviors in reproductive-aged populations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;An initial indicator pool was established based on domestic and international preconception care guidelines and literature. After discussion by the indicator development group, a preliminary framework comprising 5 first-level and 22 second-level indicators was formed. Two rounds of Delphi expert consultation were conducted from October 2022 to March 2023. The coefficient of variation, Kendall's &lt;i&gt;W&lt;/i&gt;, and statistical significance tests were used to evaluate expert consensus. Based on the consultation results, the indicators were refined and rating categories were determined. The analytic hierarchy process was applied to assign weights to indicators at each level, and a weighted composite scoring model with a total score ranging from 0 to 100 was established. The indicator system was operationalized into the \"Beiyun\" (pregnancy planning) questionnaire. Using multistage stratified sampling, reproductive-aged individuals with childbearing intentions, including those with natural pregnancy, infertility, or early miscarriage, were recruited from maternal and child health hospitals and primary healthcare facilities in Xihu and Gongshu districts of Hangzhou, as well as Jiangshan city and Kaihua county of Quzhou, Zhejiang Province, between July and September 2023. Data were collected via the Wenjuanxing online platform. Composite scores were calculated using the scoring model, and influencing factors were analyzed using analysis of variance and multiple linear regression.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;After two rounds of Delphi consultation (25 experts in the first round, 22 in the second; authority coefficients were 0.95 and 0.96, respectively), a final evaluation system was developed. The system consisted of 5 first-level indicators (pregnancy timing, healthcare-seeking behavior, health-promoting behavior, risk-avoidance behavior, and disease prevention/treatment behavior) and 26 second-level indicators, with a total score of 100. A total of 2072 valid questionnaires were collected (1120 from women, 952 from men). Among women, the median scores for the first-level indicators were 77.13, 29.43, 62.41, 74.51, and 85.88, respectively, with a composite score of 70.83±9.17. Among men, the corresponding median scores were 100.00, 39.24, 63.63, 70.32, and 83.63, with a composite score of 70.32±10.48. Educational level, self-rated health status, and planned pregnancy status were independent predictors of higher behavioral scores (all &lt;i&gt;P&lt;/i&gt;&lt;0.05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The comprehensive evaluation index system for preconception health behaviors developed in this study demonstrates good scientific rigor and practical utility. The preconception health behaviors of reproductive-aged populations require improvement, with healthcare-seeking behavior b","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"751-762"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148608111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and validation of a health information-seeking behavior questionnaire for patients with non-communicable chronic diseases].","authors":"Maidiniguli Maitikuerban, Panpan Tang, Yueying Jiang, Yue Zhao, Meijuan Lan, Yuping Zhang, Leiwen Tang","doi":"10.3724/zdxbyxb-2025-0654","DOIUrl":"10.3724/zdxbyxb-2025-0654","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a health information-seeking behavior (HISB) questionnaire for patients with non-communicable chronic diseases and evaluate its reliability and validity.</p><p><strong>Methods: </strong>Based on Wilson's information behavior model, a pre-liminary questionnaire was developed through literature review, team discussions, two rounds of expert consultations, and a pilot survey. A convenience sample of 368 patients with non-communicable chronic diseases was recruited from medical institutions in Hangzhou, Jinhua, Lishui, Ningbo, Taizhou, and Wenzhou of Zhejiang Province, between March-May 2023 and August-September 2025. The dimension of health information-seeking channels was analyzed using descriptive statistics. Item analysis for the other three dimensions was performed using item-total correlation and critical ratio methods. Reliability was evaluated using Cronbach's α coefficient and split-half reliability. Content validity was assessed by expert ratings, and structural validity was evaluated using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA).</p><p><strong>Results: </strong>The final question-naire comprised 30 items across four dimensions: health information needs, health information-seeking channels, health information search and acquisition, and health information evaluation. The overall Cronbach's α coefficient was 0.970, and the split-half reliability was 0.781. The scale-level content validity index was 0.830, and the item-level content validity index ranged from 0.890 to 1.000. EFA extracted three common factors with a cumulative variance contribution rate of 75.181%. CFA indicated a good model fit.</p><p><strong>Conclusions: </strong>The HISB questionnaire developed in this study demonstrates good reliability and validity. It can serve as a reliable and valid tool to assess HISB among patients with chronic non-communicable diseases, providing a basis for healthcare professionals to develop targeted health education interventions.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"763-773"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148608200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}