Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences最新文献

{"title":"[Evolution and influencing factors of disease burden of depres-sive disorders and depressive levels among Chinese adolescents].","authors":"Jing Zeng, Zhehan Lin, Yunting Chen, Chenggang Jin, Yue Xiao, Wen Jiang","doi":"10.3724/zdxbyxb-2025-0757","DOIUrl":"10.3724/zdxbyxb-2025-0757","url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the distribution characteristics of disability-adjusted life years (DALYs) rate for depressive disorders among Chinese adolescents, and to explore the dynamic evolution trajectory of depressive levels among Chinese adolescents and their associated influencing factors.</p><p><strong>Methods: </strong>Based on data from the Global Burden of Disease Study (GBD) 2000-2021, Joinpoint regression models were used to analyze temporal trends in the disease burden of adolescent depressive disorders. Based on data from the China Family Panel Studies (CFPS) 2012-2022, the evolution of depressive levels among Chinese adolescents was further examined. Using CFPS 2022 cross-sectional data (<i>n</i>=3259), a multivariate hierarchical regression analysis was conducted to identify influencing factors across individual, family, school, and societal dimensions, with hetero-geneity tests further stratified by gender and age group.</p><p><strong>Results: </strong>GBD data showed that the DALYs rate for depressive disorders among Chinese adolescents aged 10-<15 and 15-19 years remained consistently below the global average throughout the study period, yet both exhibited a continuous upward trend, with average annual percentage changes (AAPC) of 1.85% and 1.99% in Chinese adolescents, versus 2.30% and 1.89% globally, respectively (both <i>P</i><0.01), and females showing a faster increase than males in China. CFPS data analysis revealed that depressive levels among Chinese adolescents exhibited a fluctuating trajectory, with an initial decline followed by a subsequent increase during the observation period. The 15-19 age group consistently had higher depressive levels than the 10-<15 age group; females scored higher than males; rural residence scored higher than urban residence; and western residence scored higher than central and eastern residence. Hierarchical regression analysis showed that self-rated health, happiness, interpersonal relationships, school satisfaction, academic performance, and urban residence were protective factors (<i>β</i>=－1.104-－0.144, all <i>P</i><0.05), while academic pressure, frequency of quarrels with parents, participation in tutoring classes, and western residence were risk factors (<i>β</i>=0.236-1.101, all <i>P</i><0.05). Stratified analyses by gender revealed that self-rated health, frequency of quarrels with parents, happiness, school satisfaction, and academic pressure were significantly associated with depressive levels in both males and females (all <i>P</i><0.01), with larger effect sizes observed in females; interpersonal relationships, participation in tutoring classes, and academic performance were significantly associated with depressive levels only in females (all <i>P</i><0.05); and urban residence was significantly associated with depressive levels only in males (<i>P</i><0.05). Stratified analyses by age group revealed that self-rated health, frequency of quarrels with parents, h","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"691-702"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Associations between multimorbidity patterns and depressive symptoms among middle-aged and older adults: a latent class analysis based on CHARLS data].","authors":"Yan Sun, Caixu Zhao, Songhao Peng, Tao Zhang, Yuanyuan Liang, Yunlong Cui","doi":"10.3724/zdxbyxb-2025-0615","DOIUrl":"10.3724/zdxbyxb-2025-0615","url":null,"abstract":"<p><strong>Objectives: </strong>To examine the association between different multimorbidity patterns and depressive symptoms among middle-aged and older adults.</p><p><strong>Methods: </strong>Data were obtained from 491 middle-aged and older adults with multimorbidity in the 2020 China Health and Retirement Longitudinal Study (CHARLS). Latent class analysis (LCA) was used to identify distinct multimorbidity patterns. Models with one to four classes were fitted sequentially, and the optimal model was selected based on a comprehensive evaluation of the model fit indices and interpretability. Logistic regression was applied to assess the associations between latent classes and depressive symptoms, adjusting for sex, age, marital status, residence, income status, and number of chronic conditions.</p><p><strong>Results: </strong>The prevalence of depressive symptoms among individuals with multimorbidity was 37.1%. Three multimorbidity patterns were identified: a cardiometabolic pattern, a digestive-musculoskeletal pattern, and a respiratory pattern. After adjusting for covariates, significant differences in depressive symptoms were observed across patterns. Compared with the cardiometabolic pattern, individuals in the digestive-musculoskeletal pattern had a higher likelihood of depressive symptoms (OR=1.795, 95%CI: 1.14-2.82, <i>P</i>=0.011), while no significant difference was found for the respiratory pattern (OR=1.234, 95%CI: 0.70-2.14, <i>P</i>=0.458). In addition, having four or more chronic conditions (OR=1.996, 95%CI: 1.15-3.47, <i>P</i>=0.014) and being aged ≥70 years (OR=2.817, 95%CI: 1.24-6.68, <i>P</i>=0.015) were significantly associated with higher odds of depressive symptoms.</p><p><strong>Conclusions: </strong>Multimorbidity patterns are associated with depressive symptoms among middle-aged and older adults, among which the digestive-musculoskeletal pattern is particularly linked to a higher risk of depression.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"774-782"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148537078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Service system models for adolescent mental health in different institutional contexts and their implications for China].","authors":"Yuchen Wang, Lu Yan, Zhehan Lin, Yue Xiao, Wen Jiang","doi":"10.3724/zdxbyxb-2025-0782","DOIUrl":"10.3724/zdxbyxb-2025-0782","url":null,"abstract":"<p><p>Based on health systems and multi-level governance theories, an analytical framework of \"policy elements-implementation mechanisms-governance outcomes\" was developed, covering seven dimensions: legal safeguards, financial commitment, service networks, human resources, digital/remote support, inter-sectoral collaboration, and early prevention and intervention. Structured coding was applied to Brazil, Australia, Germany, and the United States. The coding evidence was limited to laws, strategies, budgets, and project documents issued by governments or government-authorized agencies; materials from authoritative institutions such as the World Health Organization, the Organization for Economic Co-operation and Development, the Australian Institute of Health and Welfare, and the U.S. National Center for Education Statistics; and peer-reviewed studies. The analysis revealed that Brazil developed a public health-oriented community integration pathway through the Unified Health System, the Psychosocial Care Network, and the School Health Program. However, dedicated child and adolescent mental health resources were unevenly distributed. In Australia, relatively clear institutional linkages were established among its national strategy, public funding, the headspace youth service network, eheadspace digital support, and the Be You school mental health capacity-building initiative. In Germany, a collaborative model combining social insurance and youth welfare was formed based on <i>Social Code Book</i><i>Ⅷ</i>, statutory health insurance, and a common self-governance mechanism, yet a unified, nationally coordinated school-centered youth mental health service network has still not been established. In the United States, schools have become a critical entry point for mental health services, with service provision being jointly driven by federal grants, advancing wellness and resiliency in education project, school-based mental health service subsidies, and the 988 suicide & crisis lifeline. However, insufficient funding and workforce shortages remain major constraints. Consequently, an effective adolescent mental health service system is not a replication of any single institutional model but rather the result of aligning policy elements, implementa-tion mechanisms, and institutional contexts. For China, leveraging strong government coordination and an extensive education system, a hybrid service system should be constructed, in which schools serve as key entry points, county-level and community services provide support, the medical system offers specialized backup, and digital tools play an auxiliary role. The continuity and equity of the system should be enhanced through mandated collaboration, sustainable financing, a tiered workforce, and outcome-oriented evaluation.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"703-713"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of network literacy on depression levels among young adults: an analysis based on China Family Panel Studies].","authors":"Chong Zhang, Peixin Lin","doi":"10.3724/zdxbyxb-2025-0742","DOIUrl":"10.3724/zdxbyxb-2025-0742","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of network literacy on depression levels among young adults, and to provide a reference for understanding the relationship between internet use and mental health in this population.</p><p><strong>Methods: </strong>Data from 28 312 young adults (aged 18-35) were extracted from the China Family Panel Studies (CFPS) 2014, 2018, 2020, and 2022. Depression level served as the dependent variable, network literacy (comprising network usage literacy and network awareness literacy) served as the independent variable, and multiple variables from basic demographic characteristics and socioeconomic characteristics were included as control variables. First, a panel data fixed-effects model was employed to assess the effect of network literacy on depression. Second, an instrumental variable set was constructed to address endogeneity. Finally, heterogeneity analyses were conducted to evaluate the differential effects of network literacy across subgroups.</p><p><strong>Results: </strong>The overall depression level of the sample was relatively low (the median values were mostly 0.167). Network awareness literacy was at a relatively high level (the median values were mostly 0.680), whereas network usage literacy was slightly lower (median ranging from 0.199 to 0.555). Baseline linear regression showed no significant effects for either type of network literacy on depression in young adults (both <i>P</i>>0.05). However, after introducing quadratic terms, both network usage literacy and network awareness literacy exhibited a significant inverted U-shaped relationship with depression (linear term positive, quadratic term negative; both <i>P</i><0.01). This inverted U-shaped relationship remained robust after controlling for endogeneity using a panel data fixed-effects two-stage least squares instrumental variable model. Heterogeneity analyses revealed that the inverted U-shaped effects of both literacy types were stronger in females than in males. The curvilinear effect of network usage literacy was more pronounced among young adults aged 25-35, whereas the inflection-point effect of network awareness literacy was more evident in the 18-<25 age group. Furthermore, the effects of both types of network literacy on depression were significantly stronger among rural youth than among urban youth.</p><p><strong>Conclusions: </strong>The effect of network literacy on depression levels among young adults follows an inverted U-shaped trajectory. Females, individuals aged 25-35, and rural youth are more susceptible to changes in depression levels as their network literacy fluctuates.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"723-733"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148608128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Diagnostic transitions in adolescent inpatients with mental disorders: a retrospective cohort study].","authors":"Xiang Kong, Hongjing Mao","doi":"10.3724/zdxbyxb-2025-0680","DOIUrl":"10.3724/zdxbyxb-2025-0680","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate diagnostic changes from baseline to final hospitali-zation and identify the major transition pathways among adolescent inpatients with mental disorders.</p><p><strong>Methods: </strong>Adolescents aged 12-17 years at their first hospitalization, with an interval of at least 3 years between baseline and final hospitalization and at least two complete inpatient records, were included from the electronic medical record system of the Affiliated Mental Health Center of Zhejiang University School of Medicine (Hangzhou Seventh People's Hospital). Primary discharge diagnoses were grouped according to ICD-10. Overall and diagnosis-specific diagnostic concordance rates were calculated, transition patterns were constructed, and the standardized, heatmap and Sankey diagrams were used for visualization. Multivariate logistic regression was used to explore factors associated with diagnostic inconsistency and transition from non-severe mental illness (non-SMI) at baseline to SMI. Results are presented as adjusted odds ratios (aOR) with 95% confidence intervals (CI).</p><p><strong>Results: </strong>A total of 741 patients were included. Baseline and final diagnostic categories were consistent in 409 patients (55.2%). The concordance rate was 77.5% (297/383) for baseline SMI and 31.3% (112/358) for baseline non-SMI. Reclassification within non-SMI categories was the most frequent inconsistency pattern (152 cases, 20.5%), followed by non-SMI-to-SMI transition (94 cases, 12.7%). Transitions were mainly clustered in reclassification within the affective spectrum and adjustments across the psychotic-affective boundary. Among patients with baseline depressive disorders, 61 cases were reclassified as other mood disorders, 22 cases as the combined manic episode and bipolar disorder category. Among those with baseline schizophrenia spectrum disorders, 36 cases were reclassified as the manic episode and bipolar disorder category. In multivariate models, baseline SMI was associated with lower odds of diagnostic inconsistency (aOR=0.13, 95%CI: 0.09-0.18, <i>P</i><0.01). Among patients with baseline non-SMI, older age and male were associated with higher odds of transition to SMI (aOR=1.48, 95%CI: 1.25-1.75, <i>P</i><0.01; aOR=1.99, 95%CI: 1.20-3.32, <i>P</i><0.01).</p><p><strong>Conclusions: </strong>Diagnostic transitions from baseline to final discharge were relatively concentrated, primarily involving reclassification within the affective spectrum, adjustments across the psychotic-affective boundaries, and transitions from selected non-specific non-SMI categories to SMI.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"714-722"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148608131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of exercise interventions on anxiety and depressive symptoms in patients with fibromyalgia syndrome: a meta-analysis].","authors":"Dongfei Liu, Yuanhao Li, Ping Chen, Yujiang Guo","doi":"10.3724/zdxbyxb-2025-0925","DOIUrl":"10.3724/zdxbyxb-2025-0925","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To systematically evaluate the therapeutic effects of exercise interventions on anxiety and depressive symptoms in patients with fibromyalgia syndrome (FMS), compare the differential efficacy of various exercise modalities, and provide evidence-based guidance for optimizing individualized exercise prescriptions in clinical practice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A comprehensive literature search was conducted in CNKI, VIP, Wanfang Data, SinoMed, Web of Science, PubMed, Cochrane Library, and Embase from inception to August 25, 2025. Study selection followed the PICOS framework, and methodological quality was assessed using the Cochrane risk of bias tool. Conventional pairwise meta-analysis was performed to evaluate the overall effect of exercise interventions, with heterogeneity assessed via the &lt;i&gt;I&lt;/i&gt;² statistic, and fixed-effect or random-effects models selected accordingly. Meta-regression analysis was employed to explore potential sources of hetero-geneity. Subgroup analyses were conducted to compare the efficacy of different assessment instruments and exercise prescription parameters. Network meta-analysis was used to compare the relative efficacy of different exercise modalities, with the surface under the cumulative ranking curve (SUCRA) to rank interventions. Adverse event reports were descriptively summarized. Sensitivity analyses were performed to test result robustness, and funnel plots were used to evaluate publication bias.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;A total of 30 articles (comprising 33 studies) with 1844 participants were included. Pairwise meta-analysis showed that, compared with control groups, exercise interventions significantly improved anxiety symptoms (SMD=－0.407, 95%CI: －0.515-－0.298, &lt;i&gt;P&lt;&lt;/i&gt;0.01) and depressive symptoms (SMD=－0.526, 95%CI: －0.741-－0.312, &lt;i&gt;P&lt;&lt;/i&gt;0.01) in FMS patients. For anxiety symptoms, the largest pooled effect size was observed when assessed with the state-trait anxiety inventory (SMD=－0.651, 95%CI: －0.969-－0.333), with interventions lasting more than 17 weeks (SMD=－0.771, 95%CI: －1.207-－0.278), frequency 3-4 times per week (SMD=－0.588, 95%CI: －0.812-－0.364), and session duration less than 40 min (SMD=－0.556, 95%CI: －0.855-－0.257). For depressive symptoms, the largest pooled effect size was found when assessed with the Beck depression inventory (SMD=－0.758, 95%CI: －1.186-－0.329), with interventions lasting 9-12 weeks (SMD=－0.893, 95%CI: －1.484-－0.303), frequency 3-4 times per week (SMD=－1.199, 95%CI: －1.805-－0.593), and session duration no less than 60 min (SMD=－0.708, 95%CI: －1.080-－0.336). Network meta-analysis suggested that mind-body exercise had the highest potential for improving anxiety symptoms (SUCRA=88.8%), whereas aerobic exercise appeared most promising for depressive symptoms (SUCRA=94.0%). Safety outcomes were reported in eight studies, with no serious adverse events observed. Sensitivity analyses confirmed the robustness of the findings, and pu","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"783-793"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13598569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148537106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances in mechanical signal-regulated tumor immunity and its therapeutic development].","authors":"Wenjing Xia, Liqing Dai, Jiajia Wang, Qinjie Weng","doi":"10.3724/zdxbyxb-2025-0827","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2025-0827","url":null,"abstract":"<p><p>The clinical efficacy of tumor immunotherapy is constrained by the immuno-suppressive nature of the tumor microenvironment, within which mechanical signals serve as critical modulators of immune responses. Alterations in tumor tissue mechanics-including matrix stiffening, elevated interstitial pressure, and aberrant fluid shear stress-create physical barriers that impede immune cell infiltration and cytotoxic function. Conversely, immune cells detect and interpret these mechanical cues through mechanosensi-tive pathways involving Piezo1, TRPV4, and YAP/TAZ, which regulate their activation, migration, and effector functions. Small-molecule agents targeting these pathways can precisely modulate key mechanosensitive molecules and emerging mechanical immune checkpoints, thereby bridging mechanical sensing with immune effector reprogramming. Interventions that modify microenvironmental mechanical properties-for instance, through extracellular matrix remodeling or softening-can enhance immune cell infiltration and amplify anti-tumor immunity at the tissue level. Engineered mechano-immunotherapeutic strategies, leveraging biomaterials and biomechanical principles, directly modulate membrane mechanics and mechanotransduction in immune or tumor cells to potentiate immune activity and microenvironmental responsiveness. This review synthesizes current knowledge on the mechanical properties of tumors and their microenvironments, the mechanoregulatory mechanisms operating in immune cells, and recent progress in the development of mechanically targeted therapeutics, with the goal of informing future innovation in immuno-oncology.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148841300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Advances in multi-omics research on methamphetamine-induced neurotoxicity and addiction].","authors":"Lisha Xu, Lin Miao, Jian Huang, Juan Li","doi":"10.3724/zdxbyxb-2025-0887","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2025-0887","url":null,"abstract":"<p><p>Methamphetamine (METH) is highly addictive and can cause neurotoxicity when misused over a prolonged period. METH-induced neurotoxicity and addiction involve multi-level biological alterations, including nucleotide polymorphisms, aberrant epigenetic modifications, transcriptional imbalance, protein dysfunction, metabolic dysregulation, and microbial dysbiosis with changes in microbiota-derived metabolites. To systematically dissect these multifaceted changes, genomics has been deployed to identify key gene polymorphisms linked to METH addiction susceptibility and epigenetic changes such as DNA methylation in brain regions following METH exposure. Tran-scriptomics dynamically profiles differential gene expression in addiction-relevant brain areas, particularly in pathways governing synaptic plasticity and neuroinflammation. Proteomics pinpoints the dysregulation of functional proteins associated with synaptic plasticity in response to METH. Metabolomics quantifies neurotransmitter depletion and redox imbalance induced by the drug, while microbiomics reveals gut microbiota dysbiosis and subsequent neural damage via the gut-brain axis. The integrated application of these multi-omics technologies-spanning genetic variation, transcriptional regulation, protein function, metabolic dynamics, and host-microbe interactions-systematically illuminates the complex molecular events underlying METH neurotoxicity and addiction, thereby providing a robust theoretical framework and technical roadmap for identifying early warning biomarkers and devising multi-target combination strategies against METH neurotoxicity and relapse. This review summarizes the current state of single-omics and multi-omics applications in METH neurotoxicity and addiction research, aiming to provide a reference for elucidating pathogenic mechanisms, identifying biomarkers, and discovering potential therapeutic targets.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148702381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanisms of cGAS-STING signaling pathway mediated neuroinflammation in central nervous system and their therapeutic implications].","authors":"Xinyuan Yu, Song Yao, Pinglong Xu, Ailian Wang","doi":"10.3724/zdxbyxb-2026-0036","DOIUrl":"10.3724/zdxbyxb-2026-0036","url":null,"abstract":"<p><p>The cGAS-STING signaling pathway is a crucial mechanism by which cells sense aberrant DNA and trigger type Ⅰ interferon and inflammatory responses. Emerging evidence indicates that in various central nervous system (CNS) diseases, events such as mitochondrial damage, release of mitochondrial DNA, nuclear DNA damage, lysosomal rupture, and cell death-associated DNA leakage can activate cGAS-STING signaling pathway in microglia, astrocytes, and some neurons. This activation drives transcriptional programs involving IRF3 and NF-κB, cross-talks with autophagy and cell-fate decisions, and initiates and amplifies neuroinflammation, ultimately accelerating neuronal dysfunction. This review, structured around the framework of DNA sources, signaling mechanisms, cell-fate alterations, and pathological outcomes, systematically summarizes the common mechanisms and disease-specific features of cGAS-STING in pathological conditions such as Alzheimer's disease, Parkinson's disease, ischemic stroke, multiple sclerosis, and other disorders. Furthermore, it summarizes current intervention strategies, including small-molecule inhibitors of cGAS or STING, transcriptional axis blockade, and targeted delivery technologies. It also discusses key issues such as the cell-type-specific roles of cGAS-STING regulation in the CNS, blood-brain barrier penetration, and biomarker development, thereby providing a basis for cGAS-STING-targeted intervention in neuroinflammation.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":"55 7","pages":"638-651"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical value of cerebrospinal fluid metagenomic next-generation sequencing in the diagnosis of neonatal intracranial infection].","authors":"Lingchen Li, Lizhong Du","doi":"10.3724/zdxbyxb-2025-0965","DOIUrl":"10.3724/zdxbyxb-2025-0965","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the diagnostic performance of cerebrospinal fluid metagenomic next-generation sequencing (mNGS) for neonatal intracranial infections and its value in clinical decision-making.</p><p><strong>Methods: </strong>A retrospective observational study was conducted, enrolling neonates admitted to the Children's Hospital, Zhejiang University School of Medicine from 2020 to 2025 with suspected intracranial infection who underwent cerebrospinal fluid mNGS. The sensitivity of mNGS and its concordance with cerebrospinal fluid culture-quantitative polymerase chain reaction (qPCR) were calculated. Clinical impact was evaluated using predefined criteria, and samples were categorized accordingly into positive-impact and no-impact groups to explore independent factors influencing the positive impact of mNGS on clinical decision-making.</p><p><strong>Results: </strong>Among 61 neonates with suspected intracranial infection, 48 were confirmed. Pathogens were identified in 18 cases, of which 9 were detected exclusively by mNGS, accounting for 50% of etiological diagnoses. The sensitivity of mNGS was 31.3% (95%CI: 18.7%-46.3%), higher than that of cerebrospinal fluid culture-qPCR at 18.8% (95%CI: 8.9%-32.6%), though the difference was not statistically significant (<i>P</i>=0.15). The positive and negative concordance rates between mNGS and cerebrospinal fluid culture-qPCR were 66.7% (95%CI: 29.9%-92.5%) and 76.9% (95%CI: 60.7%-88.9%), respectively. mNGS positively influenced clinical decisions in 23 patients: 12 cases with positive results guided etiological diagnosis and treatment adjustment, while 11 cases with negative results led to antibiotic de-escalation or discontinuation. Multivariate analysis identified a positive mNGS result as an independent factor associated with positive clinical impact (OR=22.127, <i>P</i><0.01).</p><p><strong>Conclusions: </strong>Cerebrospinal fluid mNGS provides positive support in etiological diagnosis and clinical decision-making for neonatal intracranial infection.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":"55 7","pages":"675-682"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}