Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences最新文献

{"title":"[Advances in pharmacological research for retinopathy of prematurity].","authors":"Yanxi Xie, Suilian Zheng, Hui Yang","doi":"10.3724/zdxbyxb-2024-0216","DOIUrl":"10.3724/zdxbyxb-2024-0216","url":null,"abstract":"<p><p>Retinopathy of prematurity (ROP) is a proliferative retinal vascular disease that threatens the vision of premature infants. Various novel drugs have demonstrated therapeutic potential for ROP by targeting signaling pathways associated with vascular endothelial growth factor (VEGF) [such as PI3K/AKT, hypoxia-inducible factor (HIF)-1α/VEGF], oxidative stress, tumor necrosis factor (TNF)-α, and Notch pathways. Propranolol, insulin-like growth factor-1, and celecoxib attenuate pathological neovascularization via the PI3K/Akt signaling pathway. Tripterine and melatonin inhibit retinal neovascularization by modulating the HIF-1α/VEGF signaling axis. Adiponectin mitigates the damage caused by oxidative stress and preserves endothelial function by enhancing endothelial nitric oxide synthase activity. Omega-3 polyunsaturated fatty acids suppress TNF-α-mediated inflammatory responses, modulate retinal development and angiogenesis, and reduce retinal neovascular lesions. DAPT, a γ-secretase inhibitor, blocks Notch signaling to suppress abnormal vascular proliferation. These agents exhibit synergistic multi-pathway anti-angiogenic effects in preclinical models and early-phase clinical trials, offering critical insights for advancing drug development and clinical translation in ROP management.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"411-421"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pachymic acid promotes brown/beige adipocyte differentiation and lipid metabolism in preadipocytes].","authors":"Kunling Chen, Xiaobing Dou, Yiyou Lin, Danyao Bai, Yangzhou Luo, Liping Zhou","doi":"10.3724/zdxbyxb-2024-0355","DOIUrl":"10.3724/zdxbyxb-2024-0355","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of pachymic acid on brown/beige adipocyte differentiation and lipid metabolism in preadipocytes.</p><p><strong>Methods: </strong>3T3-L1 MBX cells were induced to differentiate into beige adipocytes using a brown cocktail method. The impact of pachymic acid on the viability of 3T3-L1 MBX cells was evaluated using the CCK-8 assay. The formation of lipid droplets following treatment with pachymic acid was observed by oil red O staining. The mRNA expression levels of key browning genes, including uncoupling protein (Ucp) 1, the peroxisome proliferator activated receptor-γ coactivator (Pgc)-1α, and the PR domain-containing protein 16 (Prdm16), as well as the mRNA expression of sterol regulatory element-binding protein (Srebp) 1c, acetyl-coA carboxylase (Acc), fatty acid synthase (Fas), and hormone-sensitive triglyceride lipase (Hsl), adipose triglyceride lipase (Atgl), and carnitine palmitoyltransferase (Cpt) 1 were detected by quantitative reverse transcription polymerase chain reaction. The protein expression of Ucp1, Pgc-1a, and Prdm16 was detected by Western blotting.</p><p><strong>Results: </strong>The 3T3-L1 MBX cells were induced <i>in vitro</i> to form beige adipocytes with high expression of key browning genes(<i>Ucp1</i>, <i>Pgc-1α</i>, and <i>Prdm16</i>), and beige adipose-marker genes (<i>Cd137</i>, <i>Tbx1</i>, and <i>Tmem26</i>). Concentrations range of 0-80 μmol/L pachymic acid were non-cytotoxic to 3T3-L1 MBX cells. Pachymic acid treatment significantly inhibited the differentiation of 3T3-L1 MBX cells, resulting in a notable decrease in lipid accumulation. There was a marked increase in the expression of key browning genes and their proteins products, such as Ucp1, Pgc-1α, and Prdm16, while the expressions of fat synthesis-related genes <i>Srebp1c</i>, <i>Acc</i> and <i>Fas</i> were significantly decreased (all <i>P<</i>0.05). The expressions of lipolysis-related genes (<i>Hsl</i>, <i>Atgl</i>, and <i>Cpt1</i>) were significantly increased (all <i>P</i><0.05). Treatment with 20 μmol/L pachymic acid showed the most pronounced effect.</p><p><strong>Conclusions: </strong>Pachymic acid can inhibit fat synthesis and promote lipid decomposition by regulating the brown formation and lipid differentiation of preadipocytes.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"333-341"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142980092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of kidney-tonifying Chinese herbs on thymus regene-ration after rapamycin-induced degeneration in mice].","authors":"Xunuo Wen, Meiru Zhou, Fengjie Zhang, Yaoying Shu, Jianli Gao","doi":"10.3724/zdxbyxb-2024-0700","DOIUrl":"10.3724/zdxbyxb-2024-0700","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;To investigate the effect of a variety of kidney-tonifying Chinese medicines on thymus regeneration after acute degeneration in mice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Forty-eight 8-week-old male BALB/c mice were randomly divided into normal control group, model control group, negative control group, positive control group, the fructus of &lt;i&gt;Cnidium monnieri&lt;/i&gt; (L.) Cuss. group, the fructus of &lt;i&gt;Psoralea corylifolia&lt;/i&gt; (L.) group, the fructus of &lt;i&gt;Rubus chingii&lt;/i&gt; Hu group, and the tuber onion seed group, with 6 mice in each group. Except for the normal control group, mice in the other groups received intraperitoneal injections of rapamycin (1 mg·kg&lt;sup&gt;－1&lt;/sup&gt;·d&lt;sup&gt;－1&lt;/sup&gt;) for 5 consecutive days followed by 14 h of starvation to induce acute thymus degeneration. After successful modeling, in treatment groups ethanol extract of the fructus of &lt;i&gt;Cnidium monnieri&lt;/i&gt; (L.) Cuss. (0.78 g·kg&lt;sup&gt;－1&lt;/sup&gt;·d&lt;sup&gt;－1&lt;/sup&gt;), fructus of &lt;i&gt;Psoralea corylifolia&lt;/i&gt; (L.) (0.39 g·kg&lt;sup&gt;－1&lt;/sup&gt;·d&lt;sup&gt;－1&lt;/sup&gt;), fructus of &lt;i&gt;Rubus chingii&lt;/i&gt; Hu (0.78 g·kg&lt;sup&gt;－1&lt;/sup&gt;·d&lt;sup&gt;－1&lt;/sup&gt;) or the tuber onion seed(0.39 g·kg&lt;sup&gt;－1&lt;/sup&gt;·d&lt;sup&gt;－1&lt;/sup&gt;) was intraperitoneally injected once a day for 5 days; while the negative control group was given equal volume of normal saline, and the positive control group was given metformin (300 mg·kg&lt;sup&gt;－1&lt;/sup&gt;·d&lt;sup&gt;－1&lt;/sup&gt;). The grip strength was measured with a grip tester 2 h after the last administration. The pathological changes of thymus were observed by hematoxylin and eosin (HE) staining. The structure and distribution of thymic epithelial cells were observed by multiple immunofluorescence method. The proportion of T cell subsets in thymus and peripheral blood was analyzed by flow cytometry. The level of T cell receptor excision circles (&lt;i&gt;TREC&lt;/i&gt;) in the genomic DNA of mouse spleen mononuclear cells was detected by quantitative polymerase chain reaction (PCR) for evaluation of thymic output function. The expression of thymus aging- and function-related factors in the thymus tissue were detected by quantitative reverse transcription PCR. The expression of cyclin-dependent kinase inhibitor 1A (p21) and tumor protein 53 (p53) were verified by immunohistochemistry.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Rapamycin induced thymic atrophy and significantly reduced limb grip strength in mice (&lt;i&gt;P&lt;/i&gt;&lt;0.01). Compared with the negative control group, the limb grip strength of mice in the fructus of &lt;i&gt;Psoralea corylifolia&lt;/i&gt; (L.) group, the fructus of &lt;i&gt;Rubus chingii&lt;/i&gt; Hu group and the tuber onion seed group was significantly enhanced (all &lt;i&gt;P&lt;/i&gt;&lt;0.05), and the level of &lt;i&gt;TREC&lt;/i&gt; in spleen of the mice in each administration group was reduced (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Among Chinese herb medicine-treatment groups, the recovery of thymus function and tissue structure in the tuber onion seed group was most obvious. Further study showed that compared with the negative control group, the pr","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"318-332"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Association between Chinese visceral adiposity index and the risk of nephrolithiasis].","authors":"Wei Zhang, Shengqi Zheng, Tianchi Hua, Yifan Li, Qibing Fan","doi":"10.3724/zdxbyxb-2024-0373","DOIUrl":"10.3724/zdxbyxb-2024-0373","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the association between Chinese visceral adiposity index (CVAI) and the risk of nephrolithiasis.</p><p><strong>Methods: </strong>This cross-sectional study analyzed data from 78 438 Chinese adults who underwent ultrasound examinations during health screening at the Health Examination Center of Affiliated Hospital of Yangzhou University. Participants were divided into quartiles (Q1-Q4 groups) based on CVAI. Multivariate logistic regression models were utilized to evaluate the association between CVAI and nephrolithiasis risk, followed by subgroup analyses to further explore potential relationships. The performance of CVAI in predicting the risk of nephrolithiasis was evaluated using receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>Increased CVAI was significantly associated with a higher risk of nephrolithiasis, with prevalence rising from 3.36% in the Q1 group to 10.67% in the Q4 group (<i>P</i><0.01). In adjusted models, CVAI was positively correlated with the prevalence rate of nephrolithiasis (<i>OR</i>=1.002, 95%<i>CI</i>: 1.001-1.004, <i>P</i><0.01). The risks of nephrolithiasis in the Q2, Q3, and Q4 groups were 1.196-fold (95%<i>CI</i>: 1.069-1.338, <i>P</i><0.01), 1.260-fold (95%<i>CI</i>: 1.109-1.433, <i>P</i><0.01), and 1.316-fold (95%<i>CI</i>: 1.125-1.539, <i>P</i><0.01) higher than in the Q1 group, respectively. Subgroup analysis revealed that CVAI was positively associated with the risk of nephrolithiasis in male participants, individuals aged <60 years, the hypertension group, populations with or without diabetes mellitus, and the normal body mass index subgroup. Genders and age had an interaction effect on the correlation between CVAI and the risk of nephrolithiasis development (both <i>P</i><0.05). The ROC curve analysis demonstrated that CVAI exhibited superior predictive efficacy compared to waist circumference, body mass index, visceral adiposity index, weight-adjusted waist index, cardiometabolic index and body shape index, with an area under the curve of 0.622.</p><p><strong>Conclusions: </strong>In Chinese adults, CVAI is positively associated with the risk of nephrolithiasis development, which may serve as a potential predictive marker for nephrolithiasis.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"382-389"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[NIP7 upregulates the expression of ubiquitin-conjugating enzyme E2 C to promote tumor growth in anaplastic thyroid cancer].","authors":"Yingying Gong, Ziwen Fang, Yixuan Wang, Minghua Ge, Zongfu Pan","doi":"10.3724/zdxbyxb-2024-0708","DOIUrl":"10.3724/zdxbyxb-2024-0708","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the role of nucleolar pre-rRNA processing protein NIP7 (NIP7) in maintaining the malignant phenotype of anaplastic thyroid cancer (ATC) and its molecular mechanisms.</p><p><strong>Methods: </strong>NIP7 expression in ATC tissues and its gene knock-out effects in ATC cells were analyzed using gene expression microarray (GSE33630), proteome database (IPX0008941000) and the Dependency Map database, respectively. Expression and localization of NIP7 in normal thyroid cells, papillary thyroid cancer cells, and ATC cells were detected by Western blotting. Small interfering RNA (siRNA) was transfected into ATC cells, and the knockdown efficiency of NIP7 was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting. Cell proliferation was assessed by CCK-8 assay, colony formation was evaluated by colony formation assay, and tumor growth was assessed by xenograft tumor model in nude mice. SUnSET (surface sensing of translation) assay combined with co-immunoprecipitation were employed to evaluate the effect of <i>NIP7</i> silencing on ubiquitin-conjugating enzyme E2 C (UBE2C) translation. Finally, gene set enrichment analysis was used to identify shared pathways of NIP7 and UBE2C, which were validated by qRT-PCR.</p><p><strong>Results: </strong>Compared with normal tissues and papillary thyroid cancer, NIP7 was significantly upregulated in ATC tissues, and had a gene knock-out fitness effect on different ATC cell lines. The relative protein levels of NIP7 in ATC cells were significantly higher than those in normal thyroid follicular cells, and the protein was mainly expressed in the nucleus. <i>NIP7</i> silencing significantly inhibited cell proliferation and reduced colony formation. Xenograft tumor model showed that <i>NIP7</i> knockdown significantly slowed down the growth of ATC xenograft, and the tumor volume and weight were significantly lower than those in the control group (all <i>P</i><0.05). <i>NIP7</i> silencing downregulated the protein level of UBE2C, but did not affect the expression of <i>UBE2C</i> mRNA. Compared to the control group, <i>UBE2C</i> silencing significantly inhibited ATC cells proliferation (<i>P</i><0.01) and colony formation (<i>P</i><0.05). UBE2C overexpression reversed the proliferation-inhibitory effect induced by <i>NIP7</i> silencing (<i>P</i><0.01). Gene set enrichment analysis indicated that NIP7 and UBE2C were both involved in DNA replication. <i>NIP7</i> or <i>UBE2C</i> silencing could significantly downregulate the expression levels of DNA polymerase epsilon, catalytic subunit 2 and replication factor C4 in DNA replication pathway.</p><p><strong>Conclusions: </strong>NIP7 promotes ATC tumor growth by upregulating UBE2C to mediate DNA replication.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"372-381"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Feigan granules improve traditional Chinese medicine symptoms scale scores for influenza patients: a prospective clinical observational study].","authors":"Weihao Chen, Dongsheng Zheng, Shuangshuang DU, Qian Li, Guolin Wu, Dongsheng Hong, Qingwei Zhao","doi":"10.3724/zdxbyxb-2024-0559","DOIUrl":"10.3724/zdxbyxb-2024-0559","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the clinical efficacy of hospital-prepared Chinese medicine Feigan granules for influenza patients. This study has been registered at the International Traditional Medicine Clinical Trial Registry platform (ITMCTR2025000162).</p><p><strong>Methods: </strong>A prospective observational study was conducted on influenza patients who visited the Fever Clinic of the First Affiliated Hospital, Zhejiang University School of Medicine between February and March 2024. Patients were divided into the observation group (Feigan granules combined with conventional Western medicine) and the control group (conventional Western medicine). Main symptoms (including fever, cough and sore throat) and secondary symptoms (including chest tightness, poor appetite, muscle soreness and dry mouth) were evaluated with traditional Chinese medicine (TCM) symptom scale on the first day of the patient's visit and the third day after treatment. The degrees of improvement in the TCM symptom scores before and after treatment were compared using paired rank-sum test, and the differences in the overall symptom efficacy index between two groups were compared using the Wilcoxon test.</p><p><strong>Results: </strong>A total of 217 influenza patients were included. After treatment, the TCM symptom scores of both groups were significantly improved compared with those before treatment (all <i>P</i><0.01). The median differences in the main symptom score before and after treatment in the observation and the control groups were 7 points (95%<i>CI</i>: 6.0-8.0) and 6 points (95%<i>CI</i>: 6.0-8.0), respectively. The median difference in the secondary symptom score was 3 points (95%<i>CI</i>: 2.0-4.0) in both groups. The median differences in the total score were 9 points (95%<i>CI</i>: 8.0-10.5) and 8 points (95%<i>CI</i>: 7.0-10.0) in the observation and control groups, respectively. In the subgroup with an initial cough score >2, the improvement rates of total score (97.06% <i>vs</i>. 92.59%) and secondary symptoms (92.31% <i>vs</i>. 85.11%) in observation group were significantly higher than those in the control group (<i>P</i><0.05); while there was no significant difference in the improvement rate of the main symptoms (95.59% <i>vs</i>. 90.74%, <i>P</i>>0.05).</p><p><strong>Conclusions: </strong>Feigan granules can improve the TCM syndromes of influenza patients, especially for patients with more severe cough.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"289-296"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Programmed cell death in paramyxovirus infection].","authors":"Ye Liu, Yilong Wang, Zhixu He, Zhengyan Zhao","doi":"10.3724/zdxbyxb-2024-0512","DOIUrl":"10.3724/zdxbyxb-2024-0512","url":null,"abstract":"<p><p>Paramyxoviruses are important respiratory pathogens with substantial clinical relevance in pediatric infectious diseases. During infection, multiple forms of programmed cell death (PCD) may be induced, and this plays pivotal roles in viral replication, dissemination, and host immune responses, thereby profoundly influencing the viral life cycle and disease progression. On one hand, PCD facilitates the clearance of infected cells, restricts viral spread, and activates host immune defenses, thereby enhancing antiviral immunity. On the other hand, excessive or dysregulated cell death may lead to tissue damage and immune imbalance, creating a microenvironment conducive to viral replication and exacerbating disease severity. For instance, apoptosis-mediated by both extrinsic and intrinsic pathways-contributes to infection control but may also be hijacked by viruses to promote dissemination. Pyroptosis, driven by inflammasome activation, triggers lytic cell death and the release of pro-inflammatory cytokines. Necroptosis, mediated by the RIPK1-RIPK3-MLKL signaling axis, and pyroptosis both amplify innate immune responses but may concurrently induce inflammatory dysregulation. Immunogenic cell death (ICD), characterized by the release of damage-associated molecular patterns and neoantigens, activates antigen-specific immune responses and holds therapeutic potential for antiviral and antitumor interventions. Emerging evidence suggests that ferroptosis, through the modulation of iron metabolism and associated transporters, may also participate in viral replication and infected cell clearance. This review comprehensively summarizes the roles of apoptosis, pyroptosis, necroptosis, ICD, and ferroptosis in paramyxovirus infection, aiming to deepen the understanding of paramyxovirus pathogenesis and to provide insights for developing novel antiviral strategies.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"399-410"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Global disease burden of cervical cancer and the association of screening coverage with quality of disease management].","authors":"Chang Sun, Abdalle Abdi Mustafe, Bingqing Liu, Yuanying Ma, Weiguo Lyu","doi":"10.3724/zdxbyxb-2024-0585","DOIUrl":"10.3724/zdxbyxb-2024-0585","url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the global disease burden of cervical cancer and the association between screening coverage and the quality of disease management.</p><p><strong>Methods: </strong>The data of global burden of cervical cancer 2021 and the data of cervical cancer screening 2019 were obtained from IHME Global Burden of Disease (GBD) and the WHO global health observatory, respectively. The age-standardized disease burden index was calculated, the quality of care index (QCI) was determined with principal component analysis, and the correlation between QCI and cervical cancer screening coverage was examined with linear regression analysis by regions and populations.</p><p><strong>Results: </strong>The burden of cervical cancer and the quality of management exhibited significant variability across countries with differing levels of social development. The indicators of cervical cancer burden in China were close to the average level of countries with higher socio-demographic index (SDI). The global QCI was 22.22 (10.50, 35.43), and that of China was 26.30. The global screening coverage rate for cervical cancer was 42% (12%, 86%) and that in China was 31%. After adjusting for the social development level of countries, the coverage level of cervical cancer screening was associated with QCI (<i>β</i>=0.27, <i>P</i><0.01), with no difference between low and high SDI countries (<i>P</i>>0.05). The association was significantly stronger among 25-30 years old women (<i>β</i>=1.48, <i>P</i><0.05).</p><p><strong>Conclusions: </strong>There are discrepancies in both the disease burden of cervical cancer and the quality of disease management among countries with different socioeconomic levels, and there is still considerable room for improvement in China. Expanding coverage of cervical cancer screening may be an effective strategy to enhance the management quality of cervical cancer, particularly among younger women where the screening benefits are most pronounced.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"281-288"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prediction model for transformation of chronic atrophic gastritis to high-grade intraepithelial neoplasia based on traditional Chinese medicine syndrome patterns].","authors":"Xiangying Lin, Jingyao Shi, Xiaoyan Huang, Zeyu Zheng, Xiaofeng Huang, Minghan Huang","doi":"10.3724/zdxbyxb-2024-0542","DOIUrl":"10.3724/zdxbyxb-2024-0542","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a risk prediction model for the transformation of chronic atrophic gastritis to high-grade intraepithelial neoplasia (HGIN) based on traditional Chinese medicine (TCM) syndrome patterns.</p><p><strong>Methods: </strong>Clinical data of 201 chronic atrophic gastritis patients who visited the Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine and Dong'erhuan Branch between January 2022 and March 2023 were retrospectively analyzed, including 32 patients with HGIN (HGIN group) and 169 patients with moderate and severe chronic atrophic gastritis (non-HGIN group). The information of demographic characteristics, dietary habits, lifestyle factors, social and psychosocial factors, family history of tumors, medical history and comorbidities, long-term medication, endoscopic findings, histopathological examination results, as well as TCM syndrome types were collected. Potential HGIN risk factors were screened using LASSO regression, and the significant risk factors for establishing an HGIN risk prediction model were identified using logistic regression analysis. The final model was visually presented using a nomogram, and its diagnostic performance was evaluated through receiver operating characteristic curve analysis.</p><p><strong>Results: </strong>Spleen-stomach Qi deficiency was the most common TCM syndrome in both HGIN and non-HGIN groups. LASSO-logistic regression model analysis showed that heavy alcohol consumption (X1), syndrome of static blood in stomach collaterals (X2), low-grade intraepithelial neoplasia (X3), high-salt diet (X4), and age (X5) were independent risk factors related to the occurrence of HGIN, and the predictive model was <i>ln</i>［<i>P</i>/(1－<i>P</i>)］=2.159X1+2.230X2+1.664X3+2.070X4+0.122X5- 11.096. The model demonstrated good discriminative ability, calibration, and goodness-of-fit, with area under the curve values of 0.940 and 0.891 in the training and validation sets, respectively.</p><p><strong>Conclusions: </strong>The TCM syndrome of static blood in stomach collaterals shows correlation with the transformation from chronic atrophic gastritis to HGIN. The HGIN prediction model based on TCM syndrome patterns developed in the study demonstrates potential value in clinical application.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"297-306"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on glycolipid metabolism of Sertoli cell in the development of spermatogenic cell].","authors":"Shuhao Li, Liang Kong, Jingyan Liang, Tan Ma","doi":"10.3724/zdxbyxb-2024-0346","DOIUrl":"10.3724/zdxbyxb-2024-0346","url":null,"abstract":"<p><p>Sertoli cells play an important role in the process of spermatogenesis, and the abnormalities in spermatogenesis are closely related to disruptions in glycolipid metabolism. The metabolic environment of Sertoli cells is hypoxic, with glycolysis and fatty acid β-oxidation being the primary metabolic pathways. In Sertoli cells, glycolysis produces lactate to provide energy for spermatogenic cells, while fatty acid β-oxidation generates ATP. Currently, the relationship between glycolipid metabolism in Sertoli cells and spermatogenic cell development, as well as the interplay between glucose and lipid metabolism remain unclear. Various hormones, including sex hormones, can affect glucose metabolism in Sertoli cells by endocrine regulation. The activation or inhibition of signaling pathways such as AMPK, mTOR, and Akt can alter the expression levels of glycolysis-related transporter genes and the synthesis of fatty acids, thereby affecting glycolipid metabolism in Sertoli cells. Some transcription factors such as PPARγ can regulate downstream fatty acid metabolism-related genes by directly binding to their response elements and promoting the oxidation of fatty acids in Sertoli cells. In this article we elaborate on the key factors influencing glycolipid metabolism in Sertoli cells and their interconnections, as well as their potential clinical implications, offering new insights for precisely targeted treatments of male infertility.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"257-265"},"PeriodicalIF":0.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}