Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences最新文献

{"title":"Effects of kidney-tonifying Chinese herbs on thymus regen-eration after rapamycin-induced degeneration in mice.","authors":"Xunuo Wen, Meiru Zhou, Fengjie Zhang, Yaoying Shu, Jianli Gao","doi":"10.3724/zdxbyxb-2024-0700","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0700","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of a variety of kidney-tonifying Chinese medicines on thymus regeneration after rapamycin (RAPA)-induced acute degeneration in mice.</p><p><strong>Methods: </strong>Forty-eight 8-week-old male BALB/c mice (19-23 g) were randomly divided into normal control group, model control group, negative control group, positive control group, the fructus of <i>Cnidium monnieri</i> (L.) Cuss. group, the fructus of <i>Psoralea corylifolia</i> (L.) group, the fructus of <i>Rubus chingii</i> Hu group, and the tuber onion seed group, with 6 mice in each group. Except for the normal control group, mice in the other groups received intraperitoneal injections of rapamycin (1 mg·kg<sup>－1</sup>·d<sup>－1</sup>) for 5 consecutive days followed by 14 h of starvation to induce acute thymus degeneration. After successful modeling, in treatment groups ethanol extract of the fructus of <i>Cnidium monnieri</i> (L.) Cuss. (0.78 g·kg<sup>－1</sup>·d<sup>－1</sup>), fructus of <i>Psoralea corylifolia</i> (L.) (0.39 g·kg<sup>－1</sup>·d<sup>－1</sup>), fructus of <i>Rubus chingii</i> Hu (0.78 g·kg<sup>－1</sup>·d<sup>－1</sup>) and the tuber onion seed(0.39 g·kg<sup>－1</sup>·d<sup>－1</sup>) intraperi-toneally injected once a day for 5 days; while the negative control group was given with equal volume of normal saline, and the positive control group was given with metformin (300 mg·kg<sup>－1</sup>·d<sup>－1</sup>) instead. The grip strength was measured with a grip tester 2 h after the last administration. The pathological changes of thymus were observed by hematoxylin-eosin (HE) staining. The structure and distribution of thymic epithelial cells were observed by multiple immunofluorescence method. The proportion of T cell subsets in thymus and peripheral blood was analyzed by flow cytometry. The level of T cell receptor excision circles (<i>TREC</i>) in the genomic DNA of mouse spleen mononuclear cells was detected by quantitative PCR for evaluation of thymic output function. The expression levels of thymus aging-related and function-related factors in thymus tissue were detected by quantitative reverse transcription PCR. The expression levels of key factors cyclin-dependent kinase inhibitor 1A (p21) and tumor protein 53 (p53) were verified by immunohistochemistry.</p><p><strong>Results: </strong>After RAPA treatment, the mouse limb grip strength decreased (<i>P</i><0.01), the level of <i>TREC</i> in spleen was increased, the proportion of CD4<sup>+</sup>CD8<sup>+</sup> decreased (<i>P</i><0.05), and the proportion of mature CD4 single positive T cells (CD3<sup>+</sup>TCR β<sup>+</sup>CD4<sup>+</sup>CD8<sup>－</sup>) was decreased. Compared with the negative control group, the limb grip strength of mice in the fructus of <i>Psoralea corylifolia</i> (L.) group, the fructus of <i>Rubus chingii</i> Hu group and the tuber onion seed group was significantly enhanced (all <i>P</i><0.05), and the level of <i>TREC</i> in spleen of the mi","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIP7 upregulates the expression of ubiquitin-conjugating enzyme E2 C to promote tumor growth of anaplastic thyroid cancer.","authors":"Yingying Gong, Ziwen Fang, Yixuan Wang, Minghua Ge, Zongfu Pan","doi":"10.3724/zdxbyxb-2024-0708","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0708","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the role of nucleolar pre-rRNA processing protein NIP7 in maintaining the malignant phenotype of anaplastic thyroid cancer (ATC) and its molecular mechanisms.</p><p><strong>Methods: </strong>NIP7expression in ATC tissues and its gene knock-out effects in ATC cells were analyzed using the gene expression microarray (GSE33630), proteome database (IPX0008941000) and the Dependency Map database, respectively. Expression and localization of NIP7 in normal thyroid cells (Nthy-ori 3-1), papillary thyroid cancer cells (Bcpap), and ATC cells (CAL62 and 8505C) were detected by Western blot. Small interfering RNA (siRNA) was transfected into ATC cells, and the knockdown efficiency of NIP7 was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Cell proliferation was assessed by CCK-8 assay, colony formation was evaluated by colony formation assay, and tumor growth was assessed by xenograft tumor model in nude mice. SUnSET (surface sensing of translation) assay combined with co-immunoprecipitation were employed to evaluate the effect of <i>NIP7</i> silencing on ubiquitin-conjugating enzyme 2C (UBE2C) translation. Finally, gene set enrichment analysis was used to identify shared pathways of NIP7 and UBE2C, which were validated by qRT-PCR.</p><p><strong>Results: </strong>Compared with normal tissues and papillary thyroid cancer, NIP7 was significantly upregulated in ATC tissues, and had a gene knock-out fitness effect on different ATC cell lines. The relative protein levels of NIP7 in 8505C cells and CAL62 cells were significantly higher than those in normal thyroid follicular cells, and it was mainly expressed in the nucleus. <i>NIP7</i> silencing significantly inhibited the ability of cell proliferation and reduced the number of colony formation. Xenograft tumor model showed that <i>NIP7</i> knockdown significantly slowed down the growth rate of ATC xenograft tumors, and the tumor volume and weight were significantly lower than those in the control group (<i>P</i><0.05). Besides, <i>NIP7</i> silencing downregulated the protein level of UBE2C, but did not affect the expression of <i>UBE2C</i> messenger RNA. Compared to the control group, <i>UBE2C</i> silencing significantly inhibited ATC cell proliferation (<i>P</i><0.01) and colony formation (<i>P</i><0.05). Moreover, UBE2C overexpression reversed the proliferation-inhibitory effect induced by <i>NIP7</i> silencing (<i>P</i><0.01). Gene set enrichment analysis indicated that NIP7 and UBE2C were both involved in DNA replication. <i>NIP7</i> or <i>UBE2C</i> silencing could significantly downregulate the expression levels of POLE2 and RFC4 in DNA replication pathway.</p><p><strong>Conclusions: </strong>NIP7 promotes ATC tumor growth by upregulating UBE2C to mediate DNA replication.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitexin-4 ″-<i>O</i>-glucoside alleviates acetaminophen-induced acute liver injury in mice by inhibiting endoplasmic reticulum stress.","authors":"Fan Dong, Shanglei Lai, Jiannan Qiu, Xiaobing Dou","doi":"10.3724/zdxbyxb-2024-0381","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0381","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the protective effect of vitexin-4 ″-<i>O</i>-glucoside (VOG) against acetaminophen-induced acute liver injury in mice and its underlying mechanism.</p><p><strong>Methods: </strong>C57BL/6 mice were randomly divided into 4 groups: normal control group, model control group, low-dose group of VOG (30 mg/kg), and high-dose group of VOG (60 mg/kg). Acute liver injury was induced by intraperitoneal injection of acetaminophen (500 mg/kg). VOG was administrated by gavage 2 h before acetaminophen treatment. The protective effect of VOG against acute liver injury was evaluated by detecting alanine aminotransferase (ALT), azelaic aminotransferase (AST) levels and hematoxylin and eosin staining. The malondialdehyde (MDA) and catalase (CAT) and superoxide dismutase (SOD) activity in liver were detected to evaluate the hepatic oxidative stress. The expression levels of tumor necrosis factor (TNF)-α, IL-1β, and IL-6 in liver were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The expression levels of phosphorylation c-jun <i>N</i>-terminal kinease (JNK)/JNK, p-p38/p38, inositol-requiring enzyme 1 alpha (IRE-1α), X-box binding protein 1s (XBP1s), and glucose-regulated protein 78 (GRP78) in liver were detected by Western blotting. An endoplasmic reticulum stress model was established in AML-12 cells using tunicamycin. The level of cell damage was assessed using the CCK-8 assay, and the level of lactate dehydrogenase (LDH) in the cell supernatant was measured. The gene expression levels of <i>IRE</i>-<i>1α</i>, <i>XBP1s</i>, and <i>GRP78</i> in the cells were detected using qRT-PCR.</p><p><strong>Results: </strong>In the animal experiments, compared with the model control group, VOG significantly improved plasma ALT and AST levels, liver MDA levels, and SOD and CAT activities. It also reduced the gene expression levels of <i>TNF</i>-<i>α</i>, <i>IL</i>-<i>1β</i>, and <i>IL</i>-<i>6</i> in the liver, and improved the protein phosphorylation levels of JNK and p38, as well as the protein expression levels of IRE-1α, XBP1s, and GRP78. In the cell experiments, VOG pretreatment enhanced cell viability, reduced LDH release and significantly decreased the mRNA expression levels of <i>IRE</i>-<i>1α</i>, <i>XBP1s</i>, and <i>GRP78</i>.</p><p><strong>Conclusions: </strong>VOG alleviates acetaminophen-induced acute liver injury in mice by inhibiting inflammation and oxidative stress, and by modulating the protein phosphorylation levels of JNK and p38, as well as the expression levels of endoplasmic reticulum stress-related proteins.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-term outcomes of transcatheter pulmonary valve replacement with Venus-P valve in patients with moderate-to-severe pulmonary regurgitation and right ventricular systolic dysfunction.","authors":"Haiyue Xie, Wenhao Zhu, Zhiyuan Xia, Gejun Zhang","doi":"10.3724/zdxbyxb-2024-0493","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0493","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the short-term outcomes of transcatheter pulmonary valve replacement (TPVR) using the Venus-P valve in patients with moderate-to-severe pulmonary regurgitation and right ventricular systolic dysfunction (RVSD) following surgical repair of complex congenital heart disease.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on patients undergoing Venus-P valve implantation (TPVR group, <i>n</i>=28) or surgical pulmonary valve replacement (SPVR group, <i>n</i>=19) at Fuwai Hospital between February 2014 and February 2024. All patients had moderate-to-severe pulmonary regurgitation with right ventricular ejection fraction less than 45% preoperatively. Postoperative pulmonary valve function and ventricular parameters were assessed at discharge and during 6-month follow-up.</p><p><strong>Results: </strong>All procedures were successfully completed with no early mortality. At 6 months, the TPVR group demonstrated significantly lower pulmonary valve transvalvular pressure gradients compared to the SPVR group (<i>P</i><0.05). Both groups exhibited significant improvements from baseline in NYHA functional class, biventricular ejection fractions, and right ventricular end-diastolic volume index (all <i>P</i><0.05). The reduction in right ventricular end-diastolic diameter differed between the two groups (<i>P</i><0.01); however, multivariable analysis revealed no association between this difference and surgical approach (<i>β</i>=4.4, <i>P</i>>0.05). In the TPVR group, QRS duration shortened significantly postoperatively (<i>P</i><0.01), with improvements in left ventricular end-diastolic volume index and cardiac index (both <i>P</i><0.01), but these improvements did not differ significantly from the SPVR group (all <i>P</i>>0.05). During the follow-up, one patient in each group developed infective endocarditis within 1-month post-procedure; both were successfully treated with antibiotics. No other major complications were observed.</p><p><strong>Conclusions: </strong>For patients with moderate-to-severe pulmonary regurgitation and RVSD, TPVR using the Venus-P valve effectively improves short-term pulmonary valve function and ventricular performance with a favorable safety profile, which shows potential as a minimally invasive alternative to SPVR .</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New advances in pharmacological research for retinopathy of prematurity.","authors":"Yanxi Xie, Suilian Zheng, Hui Yang","doi":"10.3724/zdxbyxb-2024-0216","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0216","url":null,"abstract":"<p><p>Retinopathy of prematurity (ROP) is a proliferative retinal vascular disease that threatens the vision of premature infants. Various novel drugs have demonstrated therapeutic potential for ROP by targeting signaling pathways associated with vascular endothelial growth factor (VEGF), including PI3K/AKT, hypoxia-inducible factor (HIF)-1α/VEGF, oxidative stress, tumor necrosis factor (TNF)-α, and Notch pathways. Propranolol, insulin-like growth factor-1, and celecoxib attenuate pathological neovascularization via the PI3K/AKT signaling pathway. Tripterine and melatonin inhibit retinal neovascularization by modulating the HIF-1α/VEGF signaling axis. Adiponectin mitigates oxidative stress damage and preserves endothelial function by enhancing endothelial nitric oxide synthase activity. Omega-3 polyunsaturated fatty acids suppress TNF-α-mediated inflammatory responses, modulate retinal development and angiogenesis, and reduce retinal neovascular lesions. The γ-secretase inhibitor DAPT blocks Notch signaling to suppress abnormal vascular proliferation. These agents exhibit synergistic multi-pathway anti-angiogenic effects in preclinical models and early-phase clinical trials, offering critical insights for advancing drug development and clinical translation in ROP management.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Chinese visceral adiposity index and the risk of nephrolithiasis.","authors":"Wei Zhang, Shengqi Zheng, Tianchi Hua, Yifan Li, Qibing Fan","doi":"10.3724/zdxbyxb-2024-0373","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0373","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the association between Chinese visceral adiposity index (CVAI) and the risk of nephrolithiasis.</p><p><strong>Methods: </strong>This cross-sectional study analyzed data from 78 438 Chinese adults who underwent ultrasound examinations during health screening at the Health Examination Center of Affiliated Hospital of Yangzhou University. The subjects were divided into quartiles based on CVAI (Q1 group-Q4 group). Multivariate logistic regression models were utilized to evaluate the association between CVAI and nephrolithiasis risk, followed by subgroup analyses to further explore potential relationships. The performance of CVAI in predicting the risk of nephrolithiasis was evaluated using receiver operating characteristic (ROC) curves.</p><p><strong>Results: </strong>Increased CVAI was significantly associated with a higher risk of nephrolithiasis, with prevalence rising from 3.36% in the Q1 group to 10.67% in the Q4 group (<i>P</i><0.01). In adjusted models, CVAI was positively correlated with the prevalence rate of nephrolithiasis (<i>OR</i>=1.002, 95%<i>CI</i>: 1.001-1.004, <i>P</i><0.01). The risks of nephrolithiasis in the Q2, Q3, and Q4 groups were 1.196-fold (95%<i>CI</i>: 1.009-1.010, <i>P</i><0.01), 1.260-fold (95%<i>CI</i>: 1.109-1.433, <i>P</i><0.01), and 1.316-fold (95%<i>CI</i>: 1.125-1.539, <i>P</i><0.01) higher than that in the Q1 group, respectively. Subgroup analysis revealed that CVAI demonstrated positive correlations with the risk of nephrolithiasis in male participants, individuals aged <60 years, the hypertension group, populations with or without diabetes mellitus, and the normal body mass index subgroup. Genders and age had an interaction effect on the correlation between CVAI and the risk of nephrolithiasis development (both <i>P</i><0.05). The ROC curve analysis demonstrated that CVAI exhibited superior diagnostic efficacy compared to waist circumference, body mass index, visceral adiposity index, weight-adjusted waist index, cardiometabolic index and body shape index, with an area under the curve of 0.622.</p><p><strong>Conclusions: </strong>In Chinese adults, CVAI is positively associated with the risk of nephrolithiasis development, which may serve as a potential predictive marker for nephrolithiasis.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of programmed cell death in paramyxovirus infection.","authors":"Ye Liu, Yilong Wang, Zhixu He, Zhengyan Zhao","doi":"10.3724/zdxbyxb-2024-0512","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0512","url":null,"abstract":"<p><p>Paramyxoviruses are significant respiratory pathogens with substantial clinical relevance in pediatric infectious diseases. During infection, they induce multiple forms of programmed cell death (PCD), which play pivotal roles in viral replication, dissemination, and host immune responses, thereby profoundly influencing the viral life cycle and disease progression. On one hand, PCD facilitates the clearance of infected cells, restricts viral spread, and activates host immune defenses, thereby enhancing antiviral immunity. On the other hand, excessive or dysregulated cell death may lead to tissue damage and immune imbalance, creating a microenvironment conducive to viral replication and exacerbating disease severity. For instance, apoptosis-mediated by both intrinsic and extrinsic pathways-contributes to infection control but may also be hijacked by viruses to promote dissemination. Pyroptosis, driven by inflammasome activation, triggers lytic cell death and the release of pro-inflammatory cytokines. Necroptosis, mediated by the RIPK1-RIPK3-MLKL signaling axis, and pyroptosis both amplify innate immune responses but may concurrently induce inflammatory dysregulation. Immunogenic cell death (ICD), characterized by the release of damage-associated molecular patterns (DAMPs) and neoantigens, activates antigen-specific immune responses and holds therapeutic potential for antiviral and antitumor interventions. Emerging evidence suggests that ferroptosis, through the modulation of iron metabolism and associated transporters, may also participate in viral replication and infected cell clearance. This review comprehensively summarizes the roles of apoptosis, pyroptosis, necroptosis, ICD, and ferroptosis in paramyxovirus infection, aiming to deepen the understanding of paramyxovirus pathogenesis and provide insights for developing novel antiviral strategies, particularly in pediatric populations.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a prediction model for transformation of chronic atrophic gastritis to high-grade intraepithelial neoplasia based on Traditional Chinese Medicine syndrome patterns.","authors":"Xiangying Lin, Jingyao Shi, Xiaoyan Huang, Zeyu Zheng, Xiaofeng Huang, Minghan Huang","doi":"10.3724/zdxbyxb-2024-0542","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0542","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a risk prediction model for the transformation of chronic atrophic gastritis (CAG) to high-grade intraepithelial neoplasia (HGIN) based on Traditional Chinese Medicine (TCM) syndrome patterns.</p><p><strong>Methods: </strong>Clinical data of 201 chronic atrophic gastritis patients who visited the Department of Gastroenterology at the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine and Dong'erhuan Branch between January 2022 and March 2023 were retrospectively analyzed, including 32 patients with HGIN (HGIN group) and 169 patients with moderate and severe chronic atrophic gastritis (non-HGIN group). The information of demographic characteristics, dietary habits, lifestyle factors, psychosocial factors, family history of tumors, medical history and comorbidities, long-term medication, endoscopic findings, histopathological examination results, as well as TCM syndrome types were collected. Potential HGIN risk factors of were screened using LASSO regression, and then significant risk factors for establishing an HGIN risk prediction model were identified using logistic regression analysis. The final model was visually presented using a nomogram, and its diagnostic performance was evaluated through receiver operating characteristic curve analysis.</p><p><strong>Results: </strong>Spleen-stomach Qi deficiency was the most common TCM syndrome in both HGIN and non-HGIN groups. LASSO-logistic regression model analysis showed that heavy alcohol consumption (X1), syndrome of static blood in stomach collaterals (X2), low-grade intraepithelial neoplasia (X3), high-salt diet (X4), and age (X5) were independent risk factors related to the occurrence of HGIN, and the predictive model was I n P 1- P=2.159X1+2.23X2+1.664X3+2.07X4+0.122X5- 11.096. The model demonstrated good discriminative ability, calibration, and goodness-of-fit.</p><p><strong>Conclusions: </strong>The TCM syndrome of static blood in stomach collaterals shows a certain correlation with the transformation from chronic atrophic gastritis to HGIN. The HGIN prediction model based on TCM syndrome patterns developed in the study demonstrates potential clinical application value.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shenge powder inhibits myocardial fibrosis in rats with post-myocardial infarction heart failure through LOXL2/TGF-β1/IL-11 signaling pathway.","authors":"Hang Xie, Boyong Qiu, Haitao Li, Ruoyu Shi","doi":"10.3724/zdxbyxb-2024-0606","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0606","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effect of Shenge powder (SGP) on myocardial fibrosis in rats with heart failure after myocardial infarction and its relation with lysyl oxidase like protein 2 (LOXL2)/transforming growth factor-β1 (TGF-β1)/IL-11 signaling pathway.</p><p><strong>Methods: </strong>Seventy-two SPF male SD rats were divided into blank control group, model control group, SGP small dose group, SGP large dose group, positive control group, SGP large dose+LOXL2 activator group, with 12 rats in each group. Except for blank control group, post-myocardial infarction heart failure was induced by coronary constriction in all groups. Corresponding treatments were given immediately after successful modeling, once a day for 4 weeks. Left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) in rats were detected by Color Doppler ultrasound imaging. Levels of IL-1β and IL-6 in serum were analyzed by ELISA method, Myocardial collagen volume fraction (CVF) was evaluated by Masson staining. Expressions of collagen Ⅰ and α-smooth muscle actin (α-SMA) in myocardial tissue were detected by immunohistochemical staining. The mRNA expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in myocardial tissue were detected by qRT-PCR. Expression of LOXL2, TGF-β1, and IL-11 proteins in myocardial tissue were detected by Western blotting.</p><p><strong>Results: </strong>Compared with the blank control group, the LVFS and LVEF of the model control group decreased, the levels of serum IL-6 and IL-1β elevated, the CVF value, the expressions of collagen Ⅰ and α-SMA in myocardial tissue, expressions of <i>MMP</i>-<i>9</i> and <i>TIMP</i>-<i>1</i> mRNA, and expressions of LOXL2, TGF-β1, and IL-11 proteins increased (all <i>P</i><0.05). Compared with the model control group, the LVFS and LVEF of SGP small dose group, SGP large dose group and positive control group increased, the levels of serum IL-6 and IL-1β decreased, the CVF value, the expressions of Collagen Ⅰ and α-SMA in myocardial tissue, expressions of <i>MMP</i>-<i>9</i> and <i>TIMP</i>-<i>1</i> mRNA, and expressions of LOXL2, TGF-β1, and IL-11 proteins decreased (<i>P</i><0.05); while LOXL2 activator reversed the improvement effect of high-dose SGP on myocardial fibrosis in heart failure rats after myocardial infarction.</p><p><strong>Conclusions: </strong>Shenge powder may inhibit myocardial fibrosis in heart failure rats after myocardial infarction by inhibiting the LOXL2/TGF-β1/IL-11 pathway.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feigan granules improve traditional Chinese medicine symptoms scale scores for influenza patients: a prospective clinical observational Study.","authors":"Weihao Chen, Dongsheng Zheng, Shuangshuang DU, Qian Li, Guolin Wu, Dongsheng Hong, Qingwei Zhao","doi":"10.3724/zdxbyxb-2024-0559","DOIUrl":"https://doi.org/10.3724/zdxbyxb-2024-0559","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the clinical efficacy of hospital-prepared Chinese medicine Feigan granules for influenza patients.</p><p><strong>Methods: </strong>A prospective observational study was conducted on influenza patients who visited the Fever Clinic of the First Affiliated Hospital, Zhejiang University School of Medicine between February 2024 and March 2024 were enrolled. Patients were divided into an observation group (Feigan granules combined with conventional Western medicine) and a control group (conventional Western medicine) based on whether they used Feigan granules. Main symptoms (including fever, cough and sore throat) and secondary symptoms (including chest tightness, poor appetite, muscle soreness and dry mouth) were evaluated with traditional Chinese medicine (TCM) symptom scale on the first day of the patient's visit and the third day after treatment. The degrees of improvement in the TCM symptom scores before and after treatment were compared using the paired rank-sum test, and the differences in the overall symptom efficacy index between two groups were compared using the Wilcoxon test.</p><p><strong>Results: </strong>A total of 217 influenza patients were included. After treatment, the TCM symptom scores of both groups were significantly improved compared with those before treatment (all <i>P</i><0.05). The median difference in the main symptom score before and after treatment in the study and the control groups was 7 points (95%<i>CI</i>: 6.0-8.0) and 6 points (95%<i>CI</i>: 6.0-8.0), respectively; the median difference in the secondary symptom score was 3 points (95%<i>CI</i>: 2.0-4.0) in both groups. The median difference in the total score was 9 points (95%<i>CI</i>: 8.0-10.5) and 8 points (95%<i>CI</i>: 7.0-10.0) in the study and control groups, respectively. In the subgroup with an initial cough score >2, the improvement rates of overall symptoms (97.06% <i>vs</i>. 92.59%) and secondary symptoms (92.31% <i>vs</i>. 85.11%) in study group were significantly higher than those in the control group (<i>P</i><0.05); while there was no significant difference in the improvement rate of main symptoms (95.59% <i>vs</i>. 90.74%, <i>P</i>>0.05).</p><p><strong>Conclusions: </strong>Feigan granules can improve the TCM syndromes of influenza patients, especially for patients with more severe cough.</p>","PeriodicalId":24007,"journal":{"name":"Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences","volume":" ","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144038532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}