Zebrafish最新文献_第4页

The Effects of Plant Protein-Enriched Live Food on Larval Zebrafish Growth and the Status of Its Digestive Tract Development. 富含植物蛋白的活体食物对斑马鱼幼体生长及其消化道发育状况的影响

IF 2 4区生物学

Zebrafish Pub Date : 2022-12-01 Epub Date: 2022-11-11 DOI: 10.1089/zeb.2022.0005

Connor W Schwepe, Michal Wojno, Giovanni S Molinari, Karolina Kwasek

{"title":"The Effects of Plant Protein-Enriched Live Food on Larval Zebrafish Growth and the Status of Its Digestive Tract Development.","authors":"Connor W Schwepe, Michal Wojno, Giovanni S Molinari, Karolina Kwasek","doi":"10.1089/zeb.2022.0005","DOIUrl":"10.1089/zeb.2022.0005","url":null,"abstract":"Live food is necessary for the proper development of zebrafish larvae, providing nutrition in a form that is easily digestible and available to the larvae. Live food is commonly enriched to increase the dietary content of certain nutrients. However, little research has been done on protein-based enrichments, especially those of plant origin. This study sought to examine how different quality protein enrichments affected the composition of live food as well as growth and digestive tract development of larval zebrafish, Danio rerio. Larval zebrafish were fed from 3 to 22 days posthatch (dph) with one of six live food (rotifers Brachionus plicatilis and Artemia spp.) treatments: (1) live food with no enrichment (starved; control); (2) live food enriched with commercially used Spirulina spp. algae; (3) live food enriched with soybean meal (SBM); (4) live feed enriched with soy protein concentrate (SPC); (5) live feed enriched with a fishmeal hydrolysate; and (6) live feed enriched with intact fishmeal (FM). Proximate composition of live food was significantly affected by enrichment, in particular, protein content of rotifers was significantly increased by enrichment with SBM. Zebrafish fed SBM-enriched live food showed longer total body length than all other groups, except SPC. Zebrafish in the SBM group also showed increased gene expression of chymotrypsin in the intestine, possibly indicating improved intestinal development and extracellular digestion, which likely contributed to improved growth. Conversely, zebrafish fed hydrolysate-enriched live food showed reduced gene expression of alkaline phosphatase, possibly indicating a less developed intestinal tract, correlating with reduced growth compared to SBM group. Overall, plant protein was shown to be a promising source of live food enrichment for improving larval zebrafish growth.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10626845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone. 用17α-甲基睾酮诱导斑马鱼种群雄性化的简单方法

IF 2 4区生物学

Zebrafish Pub Date : 2022-12-01 Epub Date: 2022-11-01 DOI: 10.1089/zeb.2022.0029

Jennifer L Matthews, Joy Murphy, Andrzej Nasiadka, Zoltan M Varga

引用次数: 2

Automated Zebrafish Phenotype Pattern Recognition: 6 Years Ago, and Now. 自动斑马鱼表型模式识别:6年前和现在。

IF 2 4区生物学

Zebrafish Pub Date : 2022-12-01 DOI: 10.1089/zeb.2022.0027

Mark Schutera, Luca Rettenberger, Markus Reischl

引用次数: 0

A Simple Blood Vessel Imaging Protocol for Live Zebrafish Larva. 活体斑马鱼幼体的简单血管成像方案。

IF 2 4区生物学

Zebrafish Pub Date : 2022-10-01 DOI: 10.1089/zeb.2022.0023

S Vinoth, Velanganni Selvaraj, Hemagowri Venkatasubramanian, Kirankumar Santhakumar

引用次数: 0

Assessment of Various Standard Fish Diets on Growth and Fecundity of Platyfish (Xiphophorus maculatus) and Medaka (Oryzias latipes). 不同标准鱼类日粮对斑螯螯虾（Xiphophorus maculatus）和斑尾螯虾（Oryzias latipes）生长和繁殖力的影响。

IF 2 4区生物学

Zebrafish Pub Date : 2022-10-01 Epub Date: 2022-07-21 DOI: 10.1089/zeb.2022.0004

Crystal Russo, Merritt Drewery, Carolyn T Chang, Markita Savage, Lindsey Sanchez, Zoltan Varga, Michael L Kent, Ronald Walter, Yuan Lu

{"title":"Assessment of Various Standard Fish Diets on Growth and Fecundity of Platyfish (Xiphophorus maculatus) and Medaka (Oryzias latipes).","authors":"Crystal Russo, Merritt Drewery, Carolyn T Chang, Markita Savage, Lindsey Sanchez, Zoltan Varga, Michael L Kent, Ronald Walter, Yuan Lu","doi":"10.1089/zeb.2022.0004","DOIUrl":"10.1089/zeb.2022.0004","url":null,"abstract":"Several small freshwater fish species are utilized as models for human conditions and disease in biomedical research. Research animal diets are generally tailored to optimize growth, fecundity, and produce healthy research animals. However, a lack of reference diets presents a barrier in comparative studies between aquatic animal models and even among laboratories using the same species. Therefore, the objective of this study was to determine feeding regime and dietary effects on growth and fecundity in two commonly used freshwater fish, platyfish and medaka. From 1 through 6 months of age, platyfish and medaka were fed one of three feeding regime/diets: (1) our custom feeding regime consists of commercial flake food, beef liver paste, and live brine shrimp (CON); (2) a commercially available zebrafish diet, Gemma (GEM); and (3) a laboratory defined reference feeding regime (WAT). Weight, size, brood numbers, and survival rates for both species were measured monthly. Numbers of platyfish fry and hatch rate of medaka embryos were also determined. We observed that custom feeding regime (CON) fed platyfish and medaka grew larger, exhibited a higher survival rate, and had higher fecundity than WAT or GEM fed fish. These observations suggest that diets and regimes designed for zebrafish are not optimal to maintain platyfish or medaka. Thus, base diets, with clearly defined components and regimes, need to be developed with compositions that can be adjusted in a species-specific manner.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595639/pdf/zeb.2022.0004.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10390829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Media Conductivity and a Combination of Iodine and Sodium Hypochlorite Surface Disinfection on Zebrafish (Danio rerio) Embryo Viability and Morphology. 介质电导率和碘和次氯酸钠联合表面消毒对斑马鱼胚胎活力和形态的评估。

IF 2 4区生物学

Zebrafish Pub Date : 2022-10-01 Epub Date: 2022-10-07 DOI: 10.1089/zeb.2022.0028

Gerardo Mendoza, Adedeji A Afolalu, Kvin Lertpiriyapong, Neil S Lipman, Christine Lieggi

{"title":"Evaluation of Media Conductivity and a Combination of Iodine and Sodium Hypochlorite Surface Disinfection on Zebrafish (Danio rerio) Embryo Viability and Morphology.","authors":"Gerardo Mendoza, Adedeji A Afolalu, Kvin Lertpiriyapong, Neil S Lipman, Christine Lieggi","doi":"10.1089/zeb.2022.0028","DOIUrl":"10.1089/zeb.2022.0028","url":null,"abstract":"Embryo surface disinfection in either an iodine or sodium hypochlorite (NaOCl) solution is commonly performed on imported zebrafish embryos to decrease pathogen introduction into a facility. The impact of the consecutive use of these disinfectants and the conductivity of the culture media on embryo survival and development post-disinfection have not been evaluated. Iodine (12.5-25 ppm) is effective at eliminating several Mycobacterium species, whereas NaOCl (50-100 ppm) reduces the number of viable Pseudoloma neurophilia spores. Casper and T5D (tropical 5D wild type) embryos reared in media of differing conductivities (0-10, 100-200, 750-950, and 1500-2000 μS) with and without exposure to NaOCl 100 ppm at 6 h post-fertilization were evaluated for survival, hatching success, and morphological defects at 5 days post-fertilization. Additionally, the consecutive use of iodine (12.5 ppm for 2 min) followed by NaOCl (75 or 100 ppm for 10 min), as well as the inverse, was evaluated. Embryo survival was not impacted by embryo rearing media alone; however, survival significantly decreased when embryos were disinfected with 100 ppm NaOCl in media with a conductivity >750-950 μS. Iodine (12 ppm) and NaOCl (75 ppm) used sequentially resulted in >50% survival, whereas the use of 100 ppm NaOCl resulted in high levels of embryo mortality.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595623/pdf/zeb.2022.0028.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10517908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Cytogenetic and Sequence Analysis of U Small Nuclear RNA Genes in Three Ancistrus Species (Siluriformes: Loricariidae). 三种兔甲种U小核RNA基因的比较细胞遗传学和序列分析。

IF 2 4区生物学

Zebrafish Pub Date : 2022-10-01 Epub Date: 2022-09-13 DOI: 10.1089/zeb.2022.0040

Stephane Carolenn Quadros Schott, Larissa Glugoski, Matheus Azambuja, Orlando Moreira-Filho, Marcelo Ricardo Vicari, Viviane Nogaroto

{"title":"Comparative Cytogenetic and Sequence Analysis of U Small Nuclear RNA Genes in Three Ancistrus Species (Siluriformes: Loricariidae).","authors":"Stephane Carolenn Quadros Schott, Larissa Glugoski, Matheus Azambuja, Orlando Moreira-Filho, Marcelo Ricardo Vicari, Viviane Nogaroto","doi":"10.1089/zeb.2022.0040","DOIUrl":"https://doi.org/10.1089/zeb.2022.0040","url":null,"abstract":"Ancistrus presents a wide karyotypic diversity, resulting from numeric and structural chromosomal rearrangements. It has been proposed that some genome-specific regions containing repetitive units could organize prone-to-break DNA sites in Loricariidae, triggering chromosomal rearrangements such as Robertsonian fusions (Rb fusions), centric fissions, translocations, and inversions. The tandemly repeats of the small nuclear RNAs (snRNAs) gene families are considered good cytogenetic markers for understanding chromosomal remodeling events among closely related species, but these snRNAs have been scarcely analyzed in Ancistrus. This study presented the nucleotide sequencing and comparative in situ location of U snRNA sequences from Ancistrus aguaboensis, Ancistrus cf. multispinis, and Ancistrus sp. (2n = 50, 52, and 50, respectively), aiming to provide information about snRNA clusters in the genome and chromosome evolution in Ancistrus. U snRNA nucleotide sequences of Ancistrus presented identity to orthologous copies and folded their secondary structures correctly. In situ localization and karyotyping of the three Ancistrus species revealed clustered copies of U2 and U5 snRNA gene families to a single chromosome site, one chromosome pair bearing U1 snRNA sequence, and one main locus of U4 snRNA sequence, besides scattered signals along the chromosomes. Previous studies related the participation of the rRNA gene families in centric fusion events, contributing to chromosome rearrangements and karyotype plasticity present in Loricariidae. In this study, homeologies in U snRNA loci chromosomal locations were detected, indicating the occurrence of conserved sites of these gene families in these three Ancistrus species with 2n = 50 or 52 chromosomes.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33465463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Use of Zebrafish as a Model Organism to Study Oxidative Stress: A Review. 以斑马鱼为模式生物研究氧化应激的研究进展

IF 2 4区生物学

Zebrafish Pub Date : 2022-10-01 Epub Date: 2022-09-01 DOI: 10.1089/zeb.2021.0083

Sabarna Chowdhury, Surjya Kumar Saikia

{"title":"Use of Zebrafish as a Model Organism to Study Oxidative Stress: A Review.","authors":"Sabarna Chowdhury, Surjya Kumar Saikia","doi":"10.1089/zeb.2021.0083","DOIUrl":"https://doi.org/10.1089/zeb.2021.0083","url":null,"abstract":"Dioxygen is an integral part of every living organism, but its concentration varies from organ to organ. Production of metabolites from dioxygen may result in oxidative stress. Since oxidative stress has the potential to damage various biomolecules in the cell, therefore, it has presently become an active field of research. Oxidative stress has been studied in a wide range of model organisms from vertebrates to invertebrates, from rodents to piscine organisms, and from in vivo to in vitro models. But zebrafish (adults, larvae, or embryonic stage) emerged out to be the most promising vertebrate model organism to study oxidative stress because of its vast advantages (transparent embryo, cost-effectiveness, similarity to human genome, easy developmental processes, numerous offspring per spawning, and many more). This is evidenced by voluminous number of researches on oxidative stress in zebrafish exposed to chemicals, radiations, nanoparticles, pesticides, heavy metals, etc. On these backgrounds, this review attempts to highlight the potentiality of zebrafish as model of oxidative stress compared with other companion models. Several areas, from biomedical to environmental research, have been covered to explain it as a more convenient and reliable animal model for experimental research on oxidative mechanisms.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40336618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Telomerase Activity in Androgenetic Rainbow Trout with Growth Deficiency and in Normally Developed Individuals. 生长缺陷和正常发育个体雄激素性虹鳟的端粒酶活性。

IF 2 4区生物学

Zebrafish Pub Date : 2022-08-01 Epub Date: 2022-07-22 DOI: 10.1089/zeb.2022.0012

Ligia Panasiak, Marcin Kuciński, Agata Błaszczyk, Konrad Ocalewicz

{"title":"Telomerase Activity in Androgenetic Rainbow Trout with Growth Deficiency and in Normally Developed Individuals.","authors":"Ligia Panasiak, Marcin Kuciński, Agata Błaszczyk, Konrad Ocalewicz","doi":"10.1089/zeb.2022.0012","DOIUrl":"https://doi.org/10.1089/zeb.2022.0012","url":null,"abstract":"Role of telomerase in specimens with retarded growth (dwarfs) has not been thoroughly examined to date. Considering that some of the fish species show correlation between somatic growth and activity of telomerase, it has been tempting to assume that pattern of telomerase activity in specimens with retarded growth and these with normal growth rate may vary. In the present research, telomerase activity has been examined in liver, skin, and muscles in the androgenetic rainbow trout (Oncorhynchus mykiss) with growth deficiency and their normally developed siblings. Among the examined organs, the liver showed the highest telomerase activity in all studied fish, what may be linked to the enormous regeneration capacity of the liver tissue. Although dwarf specimens examined here displayed significantly lower body size and weight they did not exhibit any significant differences in the telomerase activity measured in liver and muscle when compared to the rainbow trout without growth deficiency. In turn, telomerase activity in skin was significantly upregulated in the normally developed androgenotes. The present study indicates that dwarfism in the androgenetic rainbow trout is neither associated with ceased telomerase activity nor its decrease throughout the ontogenetic development.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40626513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dopamine Receptors Gene Expression Pattern and Locomotor Improvement Differ Between Female and Male Zebrafish During Spinal Cord Auto Repair. 雌雄斑马鱼在脊髓自动修复过程中多巴胺受体基因表达模式和运动改善的差异

IF 2 4区生物学

Zebrafish Pub Date : 2022-08-01 Epub Date: 2022-07-29 DOI: 10.1089/zeb.2021.0081

Parastoo Hosseini, Sara Mirsadeghi, Saeid Rahmani, Amin Izadi, Mohammad Rezaei, Zahra Ghodsi, Vafa Rahimi-Movaghar, Sahar Kiani

{"title":"Dopamine Receptors Gene Expression Pattern and Locomotor Improvement Differ Between Female and Male Zebrafish During Spinal Cord Auto Repair.","authors":"Parastoo Hosseini, Sara Mirsadeghi, Saeid Rahmani, Amin Izadi, Mohammad Rezaei, Zahra Ghodsi, Vafa Rahimi-Movaghar, Sahar Kiani","doi":"10.1089/zeb.2021.0081","DOIUrl":"https://doi.org/10.1089/zeb.2021.0081","url":null,"abstract":"The dopaminergic system, a spinal cord (SC) motor circuit regulator, is administrated by sexual hormones and evolutionary conserved in all vertebrates. Accordingly, we hypothesized that the dopamine receptor (DAR) expression pattern may be dissimilar in female and male zebrafish SC auto repair. We implemented an uncomplicated method to induce spinal cord injury (SCI) on fully reproductive adult zebrafish, in both genders. SCI was induced using a 28-gauge needle at 9th-10th vertebra without skin incision. Thereupon, lesioned SC was harvested for DAR gene expression analysis; zebrafish were tracked routinely for any improvement in swim distance, speed, and their roaming capabilities/preference. Our findings revealed discrepancies between drd2a, drd2b, drd3, drd4a, and drd4b expression patterns at 1, 7, and 14 days postinjury (DPI) between female and male zebrafish. The receptors were mostly upregulated at 7 DPI in both genders, whereas drd2a and drd2b were mostly maximized in females. Surprisingly, drd3 was measured greater even in intact SC in males. In addition, female zebrafish were able to swim farther distances more accelerated, in multiple directions, by engaging more caudal muscles compared with males, of course with no statistical significance. Indeed, females were able to generate whole-body rotation and move forward using the muscles downstream to the lesion site, whereas the coordinated movement in males was accomplished by rostral muscles. In conclusion, there are differences in DAR gene expression pattern throughout SC autonomous recovery between adult female and male zebrafish, and also, female locomotion seems to ameliorate more rapidly.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40645910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3