活体斑马鱼幼体的简单血管成像方案。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-10-01 DOI:10.1089/zeb.2022.0023

S Vinoth, Velanganni Selvaraj, Hemagowri Venkatasubramanian, Kirankumar Santhakumar

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引用次数: 0

摘要

斑马鱼幼体具有优良的光学特性，是研究血管发育的简单模型系统。目前研究斑马鱼血管的方法包括利用血管特异性转基因系或专门技术，如微血管造影和视频毛细血管镜检查。在这项研究中，我们制定了一个简单的方案，使用ImageJ来观察不同发育阶段的活斑马鱼幼虫的血管，即受精后1- 4天(dpf)。为了验证这一方案，我们用100 μM槲皮素处理了1只dpf斑马鱼胚胎，并分析了槲皮素处理的3只dpf幼虫肠下静脉发育受损的情况。

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A Simple Blood Vessel Imaging Protocol for Live Zebrafish Larva.

Zebrafish larva is a simple model system to study blood vessel development because of its excellent optical properties. Current methods to study blood vessels in zebrafish involve utilizing either blood vessel-specific transgenic lines or specialized techniques such as microangiography and video capillaroscopy. In this study, we have developed a simple protocol using ImageJ to observe the blood vessels of live zebrafish larva at different developmental stages, namely 1- to 4-day postfertilization (dpf). To validate this protocol, we treated 1 dpf zebrafish embryos with 100 μM quercetin, and analyzed the impaired development of subintestinal vein in the quercetin-treated 3 dpf larvae.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.