{"title":"三种兔甲种U小核RNA基因的比较细胞遗传学和序列分析。","authors":"Stephane Carolenn Quadros Schott, Larissa Glugoski, Matheus Azambuja, Orlando Moreira-Filho, Marcelo Ricardo Vicari, Viviane Nogaroto","doi":"10.1089/zeb.2022.0040","DOIUrl":null,"url":null,"abstract":"<p><p><i>Ancistrus</i> presents a wide karyotypic diversity, resulting from numeric and structural chromosomal rearrangements. It has been proposed that some genome-specific regions containing repetitive units could organize prone-to-break DNA sites in Loricariidae, triggering chromosomal rearrangements such as Robertsonian fusions (Rb fusions), centric fissions, translocations, and inversions. The tandemly repeats of the small nuclear RNAs (snRNAs) gene families are considered good cytogenetic markers for understanding chromosomal remodeling events among closely related species, but these snRNAs have been scarcely analyzed in <i>Ancistrus</i>. This study presented the nucleotide sequencing and comparative <i>in situ</i> location of <i>U snRNA</i> sequences from <i>Ancistrus aguaboensis</i>, <i>Ancistrus</i> cf. <i>multispinis</i>, and <i>Ancistrus</i> sp. (2n = 50, 52, and 50, respectively), aiming to provide information about snRNA clusters in the genome and chromosome evolution in <i>Ancistrus</i>. <i>U snRNA</i> nucleotide sequences of <i>Ancistrus</i> presented identity to orthologous copies and folded their secondary structures correctly. <i>In situ</i> localization and karyotyping of the three <i>Ancistrus</i> species revealed clustered copies of <i>U2</i> and <i>U5 snRNA</i> gene families to a single chromosome site, one chromosome pair bearing <i>U1 snRNA</i> sequence, and one main locus of <i>U4 snRNA</i> sequence, besides scattered signals along the chromosomes. Previous studies related the participation of the <i>rRNA</i> gene families in centric fusion events, contributing to chromosome rearrangements and karyotype plasticity present in Loricariidae. In this study, homeologies in <i>U snRNA</i> loci chromosomal locations were detected, indicating the occurrence of conserved sites of these gene families in these three <i>Ancistrus</i> species with 2n = 50 or 52 chromosomes.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Comparative Cytogenetic and Sequence Analysis of <i>U Small Nuclear RNA</i> Genes in Three <i>Ancistrus</i> Species (Siluriformes: Loricariidae).\",\"authors\":\"Stephane Carolenn Quadros Schott, Larissa Glugoski, Matheus Azambuja, Orlando Moreira-Filho, Marcelo Ricardo Vicari, Viviane Nogaroto\",\"doi\":\"10.1089/zeb.2022.0040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Ancistrus</i> presents a wide karyotypic diversity, resulting from numeric and structural chromosomal rearrangements. It has been proposed that some genome-specific regions containing repetitive units could organize prone-to-break DNA sites in Loricariidae, triggering chromosomal rearrangements such as Robertsonian fusions (Rb fusions), centric fissions, translocations, and inversions. The tandemly repeats of the small nuclear RNAs (snRNAs) gene families are considered good cytogenetic markers for understanding chromosomal remodeling events among closely related species, but these snRNAs have been scarcely analyzed in <i>Ancistrus</i>. This study presented the nucleotide sequencing and comparative <i>in situ</i> location of <i>U snRNA</i> sequences from <i>Ancistrus aguaboensis</i>, <i>Ancistrus</i> cf. <i>multispinis</i>, and <i>Ancistrus</i> sp. (2n = 50, 52, and 50, respectively), aiming to provide information about snRNA clusters in the genome and chromosome evolution in <i>Ancistrus</i>. <i>U snRNA</i> nucleotide sequences of <i>Ancistrus</i> presented identity to orthologous copies and folded their secondary structures correctly. <i>In situ</i> localization and karyotyping of the three <i>Ancistrus</i> species revealed clustered copies of <i>U2</i> and <i>U5 snRNA</i> gene families to a single chromosome site, one chromosome pair bearing <i>U1 snRNA</i> sequence, and one main locus of <i>U4 snRNA</i> sequence, besides scattered signals along the chromosomes. Previous studies related the participation of the <i>rRNA</i> gene families in centric fusion events, contributing to chromosome rearrangements and karyotype plasticity present in Loricariidae. In this study, homeologies in <i>U snRNA</i> loci chromosomal locations were detected, indicating the occurrence of conserved sites of these gene families in these three <i>Ancistrus</i> species with 2n = 50 or 52 chromosomes.</p>\",\"PeriodicalId\":23872,\"journal\":{\"name\":\"Zebrafish\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2022-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zebrafish\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1089/zeb.2022.0040\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/9/13 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zebrafish","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1089/zeb.2022.0040","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/9/13 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Comparative Cytogenetic and Sequence Analysis of U Small Nuclear RNA Genes in Three Ancistrus Species (Siluriformes: Loricariidae).
Ancistrus presents a wide karyotypic diversity, resulting from numeric and structural chromosomal rearrangements. It has been proposed that some genome-specific regions containing repetitive units could organize prone-to-break DNA sites in Loricariidae, triggering chromosomal rearrangements such as Robertsonian fusions (Rb fusions), centric fissions, translocations, and inversions. The tandemly repeats of the small nuclear RNAs (snRNAs) gene families are considered good cytogenetic markers for understanding chromosomal remodeling events among closely related species, but these snRNAs have been scarcely analyzed in Ancistrus. This study presented the nucleotide sequencing and comparative in situ location of U snRNA sequences from Ancistrus aguaboensis, Ancistrus cf. multispinis, and Ancistrus sp. (2n = 50, 52, and 50, respectively), aiming to provide information about snRNA clusters in the genome and chromosome evolution in Ancistrus. U snRNA nucleotide sequences of Ancistrus presented identity to orthologous copies and folded their secondary structures correctly. In situ localization and karyotyping of the three Ancistrus species revealed clustered copies of U2 and U5 snRNA gene families to a single chromosome site, one chromosome pair bearing U1 snRNA sequence, and one main locus of U4 snRNA sequence, besides scattered signals along the chromosomes. Previous studies related the participation of the rRNA gene families in centric fusion events, contributing to chromosome rearrangements and karyotype plasticity present in Loricariidae. In this study, homeologies in U snRNA loci chromosomal locations were detected, indicating the occurrence of conserved sites of these gene families in these three Ancistrus species with 2n = 50 or 52 chromosomes.
期刊介绍:
Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease.
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage.
Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community.
TechnoFish features two types of articles:
TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines
TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community
Zebrafish coverage includes:
Comparative genomics and evolution
Molecular/cellular mechanisms of cell growth
Genetic analysis of embryogenesis and disease
Toxicological and infectious disease models
Models for neurological disorders and aging
New methods, tools, and experimental approaches
Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.