Zebrafish最新文献_第5页

TP53 Mutant Versus Wild-Type Zebrafish Larvae Under Starvation Stress: Larvae Can Live Up to 17 Days Post-Fertilization Without Food. TP53突变体与野生型斑马鱼在饥饿压力下的幼虫:幼虫在受精后不进食可存活长达17天。

IF 2 4区生物学

Zebrafish Pub Date : 2022-04-12 DOI: 10.1089/zeb.2022.0003

K. Voskarides, Constantina Koutsofti, Maria Pozova

{"title":"TP53 Mutant Versus Wild-Type Zebrafish Larvae Under Starvation Stress: Larvae Can Live Up to 17 Days Post-Fertilization Without Food.","authors":"K. Voskarides, Constantina Koutsofti, Maria Pozova","doi":"10.1089/zeb.2022.0003","DOIUrl":"https://doi.org/10.1089/zeb.2022.0003","url":null,"abstract":"In this study, an experimental protocol has been developed for comparing survival rates of mutant and wild-type zebrafish larvae under extreme starvation. Zebrafish larvae were placed in 96-well plates at fourth day postfertilization (dpf) and larvae were not fed at all from hatching to cease. Zdf1 zebrafish line was used, a strain carrying the (cancer) pathogenic TP53-M214K amino acid substitution. TP53-M214 corresponds to the human TP53-M246 and both residues are located on the DNA-binding domain of the p53 protein. Survival statistical analysis did not show any significant difference in the overall survival rates between homozygous mutant and wild-type larvae. When considering 15 dpf as the endpoint of the experiment (66% of larvae died), a borderline statistical significance was observed for the dominant model of inheritance (p = 0.015; relative hazard = 0.8320). Despite the fact yolk sac of larvae is depleted at 7-8 dpf, 34% of larvae survive until 15 dpf and 1.5% until 17 dpf. Concluding, three main results derive from this study: (1) pathogenic homozygous mutations in TP53 probably do not alter survival rates of zebrafish larvae under starvation; (2) zebrafish larvae can live up to 17 dpf without food, surviving only with their initial nutritional supplies; and (3) an easy and affordable protocol has been developed for estimating survival rates of zebrafish larvae under stressful conditions.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45608156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Cytogenetic and Molecular Characterization of Oligosarcus pintoi (Characidae): A New Record of Supernumerary Chromosome in this Species. pintoi Oligosarcus pintoi(特征性科)的细胞遗传学和分子生物学特征:该物种的额外染色体新记录。

IF 2 4区生物学

Zebrafish Pub Date : 2022-04-07 DOI: 10.1089/zeb.2021.0065

Isabelle Pereira Mari-Ribeiro, Bárbara Scorsim, A. V. D. Oliveira, A. L. Portela-Castro

{"title":"Cytogenetic and Molecular Characterization of Oligosarcus pintoi (Characidae): A New Record of Supernumerary Chromosome in this Species.","authors":"Isabelle Pereira Mari-Ribeiro, Bárbara Scorsim, A. V. D. Oliveira, A. L. Portela-Castro","doi":"10.1089/zeb.2021.0065","DOIUrl":"https://doi.org/10.1089/zeb.2021.0065","url":null,"abstract":"The genus Oligosarcus currently comprises 24 valid species distributed in the major river basins of South America. In this group, nine species were cytogenetically investigated, and found to share a diploid number of 50 chromosomes. Despite the conservation of the diploid number, variations in the karyotypic formula, number and position of the nucleolar organizer regions, and longitudinal bands have been described between both species and populations. In this study, we present cytogenetic and molecular data from Oligosarcus pintoi specimens from the Keller River, a tributary of the Ivaí River (Upper Paraná basin), using DNA barcoding and cytogenetic markers (C-band, silver-stained nucleolar organizer regions, and fluorescence in situ hybridization of 18S and 5S rDNA). The genetic inferences reached after analyzing the cytochrome c oxidade subunit 1 gene allowed us to confirm the identity of the individuals with 2n = 50 chromosomes. However, one specimen contained a medium subtelocentric supernumerary chromosome (2n = 51). This is the second record of additional chromosomes in O. pintoi, thereby confirming the existence of a supernumerary chromosome in allopatric populations of this species, a fact that demonstrates an evolutionary path that is divergent from other populations and/or species of Oligosarcus analyzed so far, contributing to the karyotypic diversification of the group.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45829349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Sound World of Zebrafish: A Critical Review of Hearing Assessment. 斑马鱼的声音世界：听力评估的批判性评论。

IF 2 4区生物学

Zebrafish Pub Date : 2022-04-01 DOI: 10.1089/zeb.2021.0063

A. Popper, J. Sisneros

{"title":"The Sound World of Zebrafish: A Critical Review of Hearing Assessment.","authors":"A. Popper, J. Sisneros","doi":"10.1089/zeb.2021.0063","DOIUrl":"https://doi.org/10.1089/zeb.2021.0063","url":null,"abstract":"Zebrafish, like all fish species, use sound to learn about their environment. Thus, human-generated (anthropogenic) sound added to the environment has the potential to disrupt the detection of biologically relevant sounds, alter behavior, impact fitness, and produce stress and other effects that can alter the well-being of animals. This review considers the bioacoustics of zebrafish in the laboratory with two goals. First, we discuss zebrafish hearing and the problems and issues that must be considered in any studies to get a clear understanding of hearing capabilities. Second, we focus on the potential effects of sounds in the tank environment and its impact on zebrafish physiology and health. To do this, we discuss underwater acoustics and the very specialized acoustics of fish tanks, in which zebrafish live and are studied. We consider what is known about zebrafish hearing and what is known about the potential impacts of tank acoustics on zebrafish and their well-being. We conclude with suggestions regarding the major gaps in what is known about zebrafish hearing as well as questions that must be explored to better understand how well zebrafish tolerate and deal with the acoustic world they live in within laboratories.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"19 2 1","pages":"37-48"},"PeriodicalIF":2.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48165928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Design and Husbandry Considerations for a Containment Level 2 Aquatic Facility. 安全壳2级水生设施的设计和管理考虑。

IF 2 4区生物学

Zebrafish Pub Date : 2022-04-01 DOI: 10.1089/zeb.2021.0086

Nicola Goodwin, Lynda Westall

引用次数: 0

Dedicated Chamber for Multimodal In Vivo Imaging of Adult Zebrafish. 成年斑马鱼体内多模态成像专用室。

IF 2 4区生物学

Zebrafish Pub Date : 2022-03-16 DOI: 10.1089/zeb.2021.0066

Steven Seeger, M. Zvolsky, Salim Melikov, Maja Frerkes, M. Rafecas

引用次数: 4

Evolutionary Dynamics of Two Classes of Repetitive DNA in the Genomes of Two Species of Elopiformes (Teleostei, Elopomorpha). 两种Elopiformes (Teleostei, Elopomorpha)基因组中两类重复DNA的进化动力学。

IF 2 4区生物学

Zebrafish Pub Date : 2022-02-01 DOI: 10.1089/zeb.2021.0027

Rodrigo Petry Corrêa de Sousa, Carolina Pinheiro Vasconcelos, Nayara Furtado do Rosário, Aldemir Branco de Oliveira-Filho, Edivaldo Herculano Corrêa de Oliveira, Marcelo de Bello Cioffi, Marcelo Vallinoto, Gláucia Caroline Silva-Oliveira

{"title":"Evolutionary Dynamics of Two Classes of Repetitive DNA in the Genomes of Two Species of Elopiformes (Teleostei, Elopomorpha).","authors":"Rodrigo Petry Corrêa de Sousa, Carolina Pinheiro Vasconcelos, Nayara Furtado do Rosário, Aldemir Branco de Oliveira-Filho, Edivaldo Herculano Corrêa de Oliveira, Marcelo de Bello Cioffi, Marcelo Vallinoto, Gláucia Caroline Silva-Oliveira","doi":"10.1089/zeb.2021.0027","DOIUrl":"https://doi.org/10.1089/zeb.2021.0027","url":null,"abstract":"The order Elopiformes includes fish species of medium to large size with a circumglobal distribution, in both the open sea, coastal, and estuarine waters. The Elopiformes are considered an excellent model for evolutionary studies due to their ample adaptive capacity, which allow them to exploit a range of different ecological niches. In this study, we analyzed the karyotype structure and distribution of two classes of repetitive DNA (microsatellites and transposable elements) in two Elopiformes species (Elops smithi and Megalops atlanticus). The results showed that the microsatellite sequences had a very similar distribution in these species, primarily associated to heterochromatin (centromeres and telomeres), suggesting these sequences contribute to the chromosome structure. In contrast, specific signals detected throughout the euchromatic regions indicate that some of these sequences may play a role in the regulation of gene expression. By contrast, the transposable elements presented a distinct distribution in the two species, pointing to a possible interspecific difference in the function of these sequences in the genomes of the two species. Therefore, the comparative genome mapping provides new insights into the structure and organization of these repetitive sequences in the Elopiformes genome.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"19 1","pages":"24-31"},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39625548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and Identification of the 20S Proteasome Complex from Zebrafish. 斑马鱼20S蛋白酶体复合物的纯化与鉴定。

IF 2 4区生物学

Zebrafish Pub Date : 2022-02-01 DOI: 10.1089/zeb.2021.0064

Ali Md Hasan, Md Maisum Sarwar Jyoti, Md Rubel Rana, Md Rezanujjaman, Toshinobu Tokumoto

{"title":"Purification and Identification of the 20S Proteasome Complex from Zebrafish.","authors":"Ali Md Hasan, Md Maisum Sarwar Jyoti, Md Rubel Rana, Md Rezanujjaman, Toshinobu Tokumoto","doi":"10.1089/zeb.2021.0064","DOIUrl":"https://doi.org/10.1089/zeb.2021.0064","url":null,"abstract":"The proteasome is a large polymeric protease complex responsible for degradation of intracellular proteins and generation of peptides. In this study, we purified a native 20S proteasome protein complex from zebrafish (Danio rerio) from the whole body. The cytosolic fraction of zebrafish hydrolyzed Suc-Leu-Leu-Val-Tyr-MCA (Suc-LLVY-MCA), a well-known substrate for the proteasome, in the presence of sodium dodecyl sulfate. From the cytosolic fraction, the 20S proteasome was purified using five column chromatography steps: DEAE cellulose, Q-Sepharose, Sephacryl S-300 gel, hydroxylapatite, and phenyl Sepharose. Electrophoresis and Western blot analyses showed that zebrafish 20S proteasome subunits have molecular masses ranging from 22 to 33 kDa. The subunit composition of the purified 20S proteasome was identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation. Fourteen kinds of 20S subunits were found. As a special characteristic of zebrafish, two proteins of the α1 subunit were identified. In addition, the results suggested that the α8 subunit is in the 20S complex instead of the α4 subunit. In this study, we demonstrated the subunit composition of the 20S proteasome complex present in zebrafish cells.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"19 1","pages":"18-23"},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39625549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration. 斑马鱼血红素加氧酶1a是正常发育和巨噬细胞迁移所必需的。

IF 2 4区生物学

Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-02-02 DOI: 10.1089/zeb.2021.0058

Kaiming Luo, Masahito Ogawa, Anita Ayer, Warwick J Britton, Roland Stocker, Kazu Kikuchi, Stefan H Oehlers

引用次数: 0

Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System. 利用活胚胎阵列系统加速神经毒理学中的化学行为表型筛选。

IF 2 4区生物学

Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-01-31 DOI: 10.1089/zeb.2021.0072

Jason Henry, Yutao Bai, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic

引用次数: 7

Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio). 斑马鱼胚胎后发育过程中血液渗透压的个体发生变化。

IF 2 4区生物学

Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-02-04 DOI: 10.1089/zeb.2021.0075

Guy Charmantier, Mai Nguyen-Chi, Georges Lutfalla

{"title":"Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio).","authors":"Guy Charmantier, Mai Nguyen-Chi, Georges Lutfalla","doi":"10.1089/zeb.2021.0075","DOIUrl":"https://doi.org/10.1089/zeb.2021.0075","url":null,"abstract":"The zebrafish Danio rerio is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different stages of zebrafish postembryonic development. We found that blood osmolality is close to 240 mOsm·kg-1 in early larvae. It progressively increased to ∼270 mOsm·kg-1 during the larval development before reaching ∼300 mOsm·kg-1 after metamorphosis in juveniles and later in adults. These ontogenetic changes in blood osmolality illustrate the physiological changes in osmoregulation associated with postembryonic development, including metamorphosis. These values are of practical interest for adjusting the osmolality of fixatives and cell and tissue culture media for research using zebrafish as a model.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"19 1","pages":"1-6"},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39896168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0