斑马鱼幼体简单、高通量致流变行为测定。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-06-03 DOI:10.1089/zeb.2021.0087

M. Tantry, V. S. Harini, Kirankumar Santhakumar

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引用次数: 1

摘要

斑马鱼（Danio rerio）被用作研究感音神经性听力损失的模型。对毛细胞的损伤可以通过对斑马鱼的流变行为进行评分来评估。在这项研究中，我们新设计了一种能够量化斑马鱼幼虫流变性行为的流变性行为测定方案。我们使用硫酸铜（一种众所周知的耳毒素）对幼虫进行化学诱导耳毒性，并测定了不同流速下的流变性。简单、经济高效、高通量的流变性测定系统可以为药物开发和其他行为研究提供深入的见解。

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Simple and High-Throughput Rheotaxis Behavioral Assay for Zebrafish Larva.

Zebrafish (Danio rerio) is used as a model for studying sensorineural hearing loss. The damage to the hair cells can be assessed by scoring rheotaxis behavior in zebrafish. In this study, we newly designed a rheotaxis behavioral assay protocol capable of quantifying rheotaxis behavior in zebrafish larvae. We chemically induced ototoxicity in the larvae using copper sulfate, a well-known ototoxin, and determined rheotaxis at different flow velocities. The simple, cost-effective, and high-throughput rheotaxis assay system can provide great insights into drug development and other behavioral studies.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.