斑马鱼幼体简单、高通量致流变行为测定。

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2022-06-03 DOI:10.1089/zeb.2021.0087

M. Tantry, V. S. Harini, Kirankumar Santhakumar

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引用次数: 1

摘要

斑马鱼（Danio rerio）被用作研究感音神经性听力损失的模型。对毛细胞的损伤可以通过对斑马鱼的流变行为进行评分来评估。在这项研究中，我们新设计了一种能够量化斑马鱼幼虫流变性行为的流变性行为测定方案。我们使用硫酸铜（一种众所周知的耳毒素）对幼虫进行化学诱导耳毒性，并测定了不同流速下的流变性。简单、经济高效、高通量的流变性测定系统可以为药物开发和其他行为研究提供深入的见解。

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Simple and High-Throughput Rheotaxis Behavioral Assay for Zebrafish Larva.

Zebrafish (Danio rerio) is used as a model for studying sensorineural hearing loss. The damage to the hair cells can be assessed by scoring rheotaxis behavior in zebrafish. In this study, we newly designed a rheotaxis behavioral assay protocol capable of quantifying rheotaxis behavior in zebrafish larvae. We chemically induced ototoxicity in the larvae using copper sulfate, a well-known ototoxin, and determined rheotaxis at different flow velocities. The simple, cost-effective, and high-throughput rheotaxis assay system can provide great insights into drug development and other behavioral studies.

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.