一种快速完全消融中性粒细胞的转基因新品系。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-06-01 DOI:10.1089/zeb.2022.0020

Christopher J Hall, Jonathan W Astin, Jeff S Mumm, David F Ackerley

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引用次数: 3

摘要

斑马鱼细胞系在特定的细胞区室中表达硝基还原酶(NTR)，使这些细胞对甲硝唑(MTZ)介导的消融敏感，已被证明在研究组织再生和研究细胞功能方面非常有用。与许多细胞相比，中性粒细胞对NTR/MTZ靶向消融策略具有相对抗性。最近，一种合理设计的NTR变体(NTR 2.0)在斑马鱼中显示出极大改善的mtz介导的消融效果。我们发现，中性粒细胞限制性表达NTR 2.0的转基因细胞系显示中性粒细胞完全消融，MTZ剂量比当前治疗方案少100倍，治疗时间短至5小时。

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A New Transgenic Line for Rapid and Complete Neutrophil Ablation.

Zebrafish lines expressing nitroreductase (NTR) in specific cell compartments, which sensitizes those cells to metronidazole (MTZ)-mediated ablation, have proven extremely useful for studying tissue regeneration and investigating cell function. In contrast to many cells, neutrophils are comparatively resistant to the NTR/MTZ targeted ablation strategy. Recently, a rationally engineered variant of NTR (NTR 2.0) has been described that exhibits greatly improved MTZ-mediated ablation efficacy in zebrafish. We show that a transgenic line with neutrophil-restricted expression of NTR 2.0 demonstrates complete neutrophil ablation, with an MTZ dose 100-fold less than current treatment regimens, and with treatment durations as short as 5 h.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.