一种快速无创提取成年斑马鱼基因组DNA的聚合酶链反应方法。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-08-01 Epub Date: 2022-07-26 DOI:10.1089/zeb.2022.0015

Ji Yun Jang, Ting Liang, Myeong-Kyu Kim, Kyung Wook Kang, Bora Lee, Seok-Yong Choi

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引用次数: 0

摘要

基因分型通常需要以基因组DNA (gDNA)为模板对聚合酶链反应(PCR)的产物进行分析，并用于突变体或转基因生物的验证。对于成年斑马鱼的基因分型，gDNA通常是从剪断的尾鳍或皮肤粘液中提取，要么用NaOH碱性裂解，要么用蛋白酶K (PK)处理。选择乙醇沉淀法进一步纯化gDNA。为了建立一种快速、无创的提取成年斑马鱼pcr基因的方法，我们将皮肤拭子与PK处理相结合，并验证了其效率。这种方法可以应用于各种各样的鱼类。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.

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A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.

Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from clipped caudal fin or skin mucus through either alkaline lysis using NaOH or proteinase K (PK) treatment. Further purification of the gDNA using ethanol precipitation was optional. To develop a rapid and noninvasive method that extracts PCR-ready gDNA from adult zebrafish, we combined skin swabbing with PK treatment and demonstrated its efficiency. This method could be applied to a wide range of fish.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.