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Design and Husbandry Considerations for a Containment Level 2 Aquatic Facility. 安全壳2级水生设施的设计和管理考虑。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-04-01 DOI: 10.1089/zeb.2021.0086
Nicola Goodwin, Lynda Westall
{"title":"Design and Husbandry Considerations for a Containment Level 2 Aquatic Facility.","authors":"Nicola Goodwin, Lynda Westall","doi":"10.1089/zeb.2021.0086","DOIUrl":"https://doi.org/10.1089/zeb.2021.0086","url":null,"abstract":"The greatly increased use of aquatic species to study disease over the past 20 years necessitates understanding their husbandry and housing requirements to optimize research and welfare and to ensure compliance with regulations. To achieve these goals, aquatic systems have expanded from pet shop and home aquaria to research-grade systems incorporating designs and features to increase their robustness, practicality, and flexibility. Moreover, these last decades have seen the increasing use of aquatic animals for infectious disease research using containment level 2 (CL2)/biosafety level 2 pathogens. In this study, we discuss the facility design requirements and modifications, which must be considered for the planning, construction, and use of an aquatic facility for zebrafish infected with CL2 pathogens. These include decontamination of water and equipment, racking and filtration design, personal protective equipment, and husbandry procedures. This guidance is based on our experience in the design and ongoing management of such facilities.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070741/pdf/zeb.2021.0086.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Sound World of Zebrafish: A Critical Review of Hearing Assessment. 斑马鱼的声音世界：听力评估的批判性评论。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-04-01 DOI: 10.1089/zeb.2021.0063
A. Popper, J. Sisneros
{"title":"The Sound World of Zebrafish: A Critical Review of Hearing Assessment.","authors":"A. Popper, J. Sisneros","doi":"10.1089/zeb.2021.0063","DOIUrl":"https://doi.org/10.1089/zeb.2021.0063","url":null,"abstract":"Zebrafish, like all fish species, use sound to learn about their environment. Thus, human-generated (anthropogenic) sound added to the environment has the potential to disrupt the detection of biologically relevant sounds, alter behavior, impact fitness, and produce stress and other effects that can alter the well-being of animals. This review considers the bioacoustics of zebrafish in the laboratory with two goals. First, we discuss zebrafish hearing and the problems and issues that must be considered in any studies to get a clear understanding of hearing capabilities. Second, we focus on the potential effects of sounds in the tank environment and its impact on zebrafish physiology and health. To do this, we discuss underwater acoustics and the very specialized acoustics of fish tanks, in which zebrafish live and are studied. We consider what is known about zebrafish hearing and what is known about the potential impacts of tank acoustics on zebrafish and their well-being. We conclude with suggestions regarding the major gaps in what is known about zebrafish hearing as well as questions that must be explored to better understand how well zebrafish tolerate and deal with the acoustic world they live in within laboratories.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48165928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Dedicated Chamber for Multimodal In Vivo Imaging of Adult Zebrafish. 成年斑马鱼体内多模态成像专用室。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-03-16 DOI: 10.1089/zeb.2021.0066
Steven Seeger, M. Zvolsky, Salim Melikov, Maja Frerkes, M. Rafecas
{"title":"Dedicated Chamber for Multimodal In Vivo Imaging of Adult Zebrafish.","authors":"Steven Seeger, M. Zvolsky, Salim Melikov, Maja Frerkes, M. Rafecas","doi":"10.1089/zeb.2021.0066","DOIUrl":"https://doi.org/10.1089/zeb.2021.0066","url":null,"abstract":"In vivo imaging of adult zebrafish is challenging, particularly for dynamic or long acquisitions when using, for example, positron emission tomography, single photon emission computed tomography, computed tomography (CT), or magnetic resonance imaging (MRI). An aqueous environment is indispensable to ensure animal welfare, but commercial small-animal imaging chambers do not provide such conditions, as they are designed for rodents. In this study we present a dedicated flow-through chamber that includes fish immobilization and allows for the continuous supply of fresh water and anesthetics, as well as the removal of excretions. Both flow simulations and experiments, as well as first scans with MRI and CT, support the suitability of the chamber.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45567864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Evolutionary Dynamics of Two Classes of Repetitive DNA in the Genomes of Two Species of Elopiformes (Teleostei, Elopomorpha). 两种Elopiformes (Teleostei, Elopomorpha)基因组中两类重复DNA的进化动力学。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-02-01 DOI: 10.1089/zeb.2021.0027
Rodrigo Petry Corrêa de Sousa, Carolina Pinheiro Vasconcelos, Nayara Furtado do Rosário, Aldemir Branco de Oliveira-Filho, Edivaldo Herculano Corrêa de Oliveira, Marcelo de Bello Cioffi, Marcelo Vallinoto, Gláucia Caroline Silva-Oliveira
{"title":"Evolutionary Dynamics of Two Classes of Repetitive DNA in the Genomes of Two Species of Elopiformes (Teleostei, Elopomorpha).","authors":"Rodrigo Petry Corrêa de Sousa,&nbsp;Carolina Pinheiro Vasconcelos,&nbsp;Nayara Furtado do Rosário,&nbsp;Aldemir Branco de Oliveira-Filho,&nbsp;Edivaldo Herculano Corrêa de Oliveira,&nbsp;Marcelo de Bello Cioffi,&nbsp;Marcelo Vallinoto,&nbsp;Gláucia Caroline Silva-Oliveira","doi":"10.1089/zeb.2021.0027","DOIUrl":"https://doi.org/10.1089/zeb.2021.0027","url":null,"abstract":"<p><p>The order Elopiformes includes fish species of medium to large size with a circumglobal distribution, in both the open sea, coastal, and estuarine waters. The Elopiformes are considered an excellent model for evolutionary studies due to their ample adaptive capacity, which allow them to exploit a range of different ecological niches. In this study, we analyzed the karyotype structure and distribution of two classes of repetitive DNA (microsatellites and transposable elements) in two Elopiformes species (<i>Elops smithi</i> and <i>Megalops atlanticus</i>). The results showed that the microsatellite sequences had a very similar distribution in these species, primarily associated to heterochromatin (centromeres and telomeres), suggesting these sequences contribute to the chromosome structure. In contrast, specific signals detected throughout the euchromatic regions indicate that some of these sequences may play a role in the regulation of gene expression. By contrast, the transposable elements presented a distinct distribution in the two species, pointing to a possible interspecific difference in the function of these sequences in the genomes of the two species. Therefore, the comparative genome mapping provides new insights into the structure and organization of these repetitive sequences in the Elopiformes genome.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39625548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and Identification of the 20S Proteasome Complex from Zebrafish. 斑马鱼20S蛋白酶体复合物的纯化与鉴定。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-02-01 DOI: 10.1089/zeb.2021.0064
Ali Md Hasan, Md Maisum Sarwar Jyoti, Md Rubel Rana, Md Rezanujjaman, Toshinobu Tokumoto
{"title":"Purification and Identification of the 20S Proteasome Complex from Zebrafish.","authors":"Ali Md Hasan,&nbsp;Md Maisum Sarwar Jyoti,&nbsp;Md Rubel Rana,&nbsp;Md Rezanujjaman,&nbsp;Toshinobu Tokumoto","doi":"10.1089/zeb.2021.0064","DOIUrl":"https://doi.org/10.1089/zeb.2021.0064","url":null,"abstract":"<p><p>The proteasome is a large polymeric protease complex responsible for degradation of intracellular proteins and generation of peptides. In this study, we purified a native 20S proteasome protein complex from zebrafish (<i>Danio rerio</i>) from the whole body. The cytosolic fraction of zebrafish hydrolyzed Suc-Leu-Leu-Val-Tyr-MCA (Suc-LLVY-MCA), a well-known substrate for the proteasome, in the presence of sodium dodecyl sulfate. From the cytosolic fraction, the 20S proteasome was purified using five column chromatography steps: DEAE cellulose, Q-Sepharose, Sephacryl S-300 gel, hydroxylapatite, and phenyl Sepharose. Electrophoresis and Western blot analyses showed that zebrafish 20S proteasome subunits have molecular masses ranging from 22 to 33 kDa. The subunit composition of the purified 20S proteasome was identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation. Fourteen kinds of 20S subunits were found. As a special characteristic of zebrafish, two proteins of the α1 subunit were identified. In addition, the results suggested that the α8 subunit is in the 20S complex instead of the α4 subunit. In this study, we demonstrated the subunit composition of the 20S proteasome complex present in zebrafish cells.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39625549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration. 斑马鱼血红素加氧酶1a是正常发育和巨噬细胞迁移所必需的。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-02-02 DOI: 10.1089/zeb.2021.0058
Kaiming Luo, Masahito Ogawa, Anita Ayer, Warwick J Britton, Roland Stocker, Kazu Kikuchi, Stefan H Oehlers
{"title":"Zebrafish <i>Heme Oxygenase 1a</i> Is Necessary for Normal Development and Macrophage Migration.","authors":"Kaiming Luo,&nbsp;Masahito Ogawa,&nbsp;Anita Ayer,&nbsp;Warwick J Britton,&nbsp;Roland Stocker,&nbsp;Kazu Kikuchi,&nbsp;Stefan H Oehlers","doi":"10.1089/zeb.2021.0058","DOIUrl":"https://doi.org/10.1089/zeb.2021.0058","url":null,"abstract":"<p><p>Heme oxygenase function is highly conserved between vertebrates where it plays important roles in normal embryonic development and controls oxidative stress. Expression of the zebrafish heme oxygenase 1 genes is known to be responsive to oxidative stress suggesting a conserved physiological function. In this study, we generate a knockout allele of zebrafish <i>hmox1a</i> and characterize the effects of <i>hmox1a</i> and <i>hmox1b</i> loss on embryonic development. We find that loss of <i>hmox1a</i> or <i>hmox1b</i> causes developmental defects in only a minority of embryos, in contrast to <i>Hmox1</i> gene deletions in mice that cause loss of most embryos. Using a tail wound inflammation assay we find a conserved role for <i>hmox1a</i>, but not <i>hmox1b</i>, in normal macrophage migration to the wound site. Together our results indicate that zebrafish <i>hmox1a</i> has clearly a partitioned role from <i>hmox1b</i> that is more consistent with conserved functions of mammalian Heme oxygenase 1.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39582482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio). 斑马鱼胚胎后发育过程中血液渗透压的个体发生变化。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-02-04 DOI: 10.1089/zeb.2021.0075
Guy Charmantier, Mai Nguyen-Chi, Georges Lutfalla
{"title":"Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (<i>Danio rerio</i>).","authors":"Guy Charmantier,&nbsp;Mai Nguyen-Chi,&nbsp;Georges Lutfalla","doi":"10.1089/zeb.2021.0075","DOIUrl":"https://doi.org/10.1089/zeb.2021.0075","url":null,"abstract":"<p><p>The zebrafish <i>Danio rerio</i> is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different stages of zebrafish postembryonic development. We found that blood osmolality is close to 240 mOsm·kg<sup>-1</sup> in early larvae. It progressively increased to ∼270 mOsm·kg<sup>-1</sup> during the larval development before reaching ∼300 mOsm·kg<sup>-1</sup> after metamorphosis in juveniles and later in adults. These ontogenetic changes in blood osmolality illustrate the physiological changes in osmoregulation associated with postembryonic development, including metamorphosis. These values are of practical interest for adjusting the osmolality of fixatives and cell and tissue culture media for research using zebrafish as a model.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39896168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System. 利用活胚胎阵列系统加速神经毒理学中的化学行为表型筛选。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-01-31 DOI: 10.1089/zeb.2021.0072
Jason Henry, Yutao Bai, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic
{"title":"Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System.","authors":"Jason Henry,&nbsp;Yutao Bai,&nbsp;Florian Kreuder,&nbsp;David Mawdsley,&nbsp;Jan Kaslin,&nbsp;Donald Wlodkowic","doi":"10.1089/zeb.2021.0072","DOIUrl":"https://doi.org/10.1089/zeb.2021.0072","url":null,"abstract":"<p><p>Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack of effective embryo in-test positioning, wide-field imaging, and high-throughput bioinformatic analytics. In this study, we demonstrate advantages of using custom large-density embryo arrays in conjunction with an open-source ultra-high-definition video imaging system. Moreover, we present a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of specimens in photomotor response bioassays. The system validation was showcased in a proof-of-concept neurotoxicity analysis.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39737130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Acknowledgment of Reviewers 2021. 2021年评审员致谢。
IF 2 4区 生物学
Zebrafish Pub Date : 2022-01-31 DOI: 10.1089/zeb.2021.29007.ack
{"title":"Acknowledgment of Reviewers 2021.","authors":"","doi":"10.1089/zeb.2021.29007.ack","DOIUrl":"https://doi.org/10.1089/zeb.2021.29007.ack","url":null,"abstract":"","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2022-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49559298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sex-Dependent Inheritance of B Chromosomes in Psalidodon paranae (Teleostei, Characiformes) Revealed by Directed Crossings. 用定向杂交揭示的paridodon parae (Teleostei，性状)B染色体的性别依赖遗传。
IF 2 4区 生物学
Zebrafish Pub Date : 2021-12-01 DOI: 10.1089/zeb.2021.0053
Caio Augusto Gomes Goes, Duílio Mazzoni Zerbinato de Andrade Silva, Ricardo Utsunomia, Nivaldo Ferreira do Nascimento, George Shigueki Yasui, José Augusto Senhorini, Diogo Teruo Hashimoto, Roberto Ferreira Artoni, Fausto Foresti, Fábio Porto-Foresti
{"title":"Sex-Dependent Inheritance of B Chromosomes in <i>Psalidodon paranae</i> (Teleostei, Characiformes) Revealed by Directed Crossings.","authors":"Caio Augusto Gomes Goes,&nbsp;Duílio Mazzoni Zerbinato de Andrade Silva,&nbsp;Ricardo Utsunomia,&nbsp;Nivaldo Ferreira do Nascimento,&nbsp;George Shigueki Yasui,&nbsp;José Augusto Senhorini,&nbsp;Diogo Teruo Hashimoto,&nbsp;Roberto Ferreira Artoni,&nbsp;Fausto Foresti,&nbsp;Fábio Porto-Foresti","doi":"10.1089/zeb.2021.0053","DOIUrl":"https://doi.org/10.1089/zeb.2021.0053","url":null,"abstract":"<p><p>B chromosomes are additional dispensable elements to the standard chromosomal set of an organism. In most cases, their transmission differs from Mendelian patterns, leading to their accumulation or extinction. The present study aimed to describe, for the first time, the transmission pattern of B chromosome in a population of <i>Psalidodon paranae</i> through directed crosses, as well as to analyze the populational dynamics of B chromosome. Our results revealed the possible elimination of B chromosome in crossings where only females were B-carriers, with a mean transmission rate (<i>k</i><sub>B</sub>) of 0.149; however, <i>k</i><sub>B</sub> was significantly higher in crossings involving male B-carriers (<i>k</i><sub>B</sub> = 0.328-0.450). Moreover, we observed an increase in the frequency of B chromosomes in the natural population of <i>P. paranae</i> in the last two decades. These apparently contradictory results can make sense if the B chromosome provides adaptive advantages to their carriers. Here, we observed a differential transmission of B chromosomes in each sex of parental individuals, with higher transmission rates in crossing involving males B-carriers, in addition to describe the temporal changes of B chromosome frequency in <i>P. paranae</i>.</p>","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39746096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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