Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio).

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-02-01 Epub Date: 2022-02-04 DOI:10.1089/zeb.2021.0075

Guy Charmantier, Mai Nguyen-Chi, Georges Lutfalla

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Abstract

The zebrafish Danio rerio is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different stages of zebrafish postembryonic development. We found that blood osmolality is close to 240 mOsm·kg^-1 in early larvae. It progressively increased to ∼270 mOsm·kg^-1 during the larval development before reaching ∼300 mOsm·kg^-1 after metamorphosis in juveniles and later in adults. These ontogenetic changes in blood osmolality illustrate the physiological changes in osmoregulation associated with postembryonic development, including metamorphosis. These values are of practical interest for adjusting the osmolality of fixatives and cell and tissue culture media for research using zebrafish as a model.

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斑马鱼胚胎后发育过程中血液渗透压的个体发生变化。

斑马鱼是一种硬骨鱼模型物种，广泛应用于发育遗传学、生物医学研究、毒理学和药物筛选等领域。尽管这个物种在研究中很有兴趣，但通过间接观察它的血液渗透压，这是各种实验的关键参数，我们对它知之甚少。在这项研究中，我们使用纳米渗透法直接测量了斑马鱼胚胎后发育不同阶段的血液渗透压。我们发现幼虫早期的血液渗透压接近240 mOsm·kg-1。在幼虫发育过程中逐渐增加到~ 270 mOsm·kg-1，在稚虫和成虫蜕变后达到~ 300 mOsm·kg-1。血液渗透压的这些个体发生变化说明了与胚胎后发育(包括变态)相关的渗透调节的生理变化。这些值对于调整固定液和细胞和组织培养基的渗透压，以斑马鱼为模型进行研究具有实际意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.