Ontogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio).

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-02-04 DOI:10.1089/zeb.2021.0075

Guy Charmantier, Mai Nguyen-Chi, Georges Lutfalla

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Abstract

The zebrafish Danio rerio is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different stages of zebrafish postembryonic development. We found that blood osmolality is close to 240 mOsm·kg^-1 in early larvae. It progressively increased to ∼270 mOsm·kg^-1 during the larval development before reaching ∼300 mOsm·kg^-1 after metamorphosis in juveniles and later in adults. These ontogenetic changes in blood osmolality illustrate the physiological changes in osmoregulation associated with postembryonic development, including metamorphosis. These values are of practical interest for adjusting the osmolality of fixatives and cell and tissue culture media for research using zebrafish as a model.

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斑马鱼胚胎后发育过程中血液渗透压的个体发生变化。

斑马鱼是一种硬骨鱼模型物种，广泛应用于发育遗传学、生物医学研究、毒理学和药物筛选等领域。尽管这个物种在研究中很有兴趣，但通过间接观察它的血液渗透压，这是各种实验的关键参数，我们对它知之甚少。在这项研究中，我们使用纳米渗透法直接测量了斑马鱼胚胎后发育不同阶段的血液渗透压。我们发现幼虫早期的血液渗透压接近240 mOsm·kg-1。在幼虫发育过程中逐渐增加到~ 270 mOsm·kg-1，在稚虫和成虫蜕变后达到~ 300 mOsm·kg-1。血液渗透压的这些个体发生变化说明了与胚胎后发育(包括变态)相关的渗透调节的生理变化。这些值对于调整固定液和细胞和组织培养基的渗透压，以斑马鱼为模型进行研究具有实际意义。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.