利用活胚胎阵列系统加速神经毒理学中的化学行为表型筛选。

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2022-02-01 Epub Date: 2022-01-31 DOI:10.1089/zeb.2021.0072

Jason Henry, Yutao Bai, Florian Kreuder, David Mawdsley, Jan Kaslin, Donald Wlodkowic

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引用次数: 7

摘要

大规模的斑马鱼胚胎化学行为表型分析是加速神经毒性测试和发现行为改变神经药物的有希望的途径。这些策略由于缺乏有效的胚胎测试定位、宽视场成像和高通量生物信息学分析而受到阻碍。在这项研究中，我们展示了将定制大密度胚胎阵列与开源超高清视频成像系统结合使用的优势。此外，我们提出了一个高通量的生物信息学工作流程，用于在光度反应生物测定中对大量标本进行快速行为分析。系统验证在概念验证神经毒性分析中得到展示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Accelerating Chemobehavioral Phenotypic Screening in Neurotoxicology Using a Living Embryo Array System.

Large-scale chemobehavioral phenotyping with zebrafish embryos is a promising avenue for accelerated neurotoxicity testing and discovery of behavior-modifying neuroceuticals. These strategies are hampered by lack of effective embryo in-test positioning, wide-field imaging, and high-throughput bioinformatic analytics. In this study, we demonstrate advantages of using custom large-density embryo arrays in conjunction with an open-source ultra-high-definition video imaging system. Moreover, we present a high-throughput bioinformatics workflow for rapid behavioral analysis of large cohorts of specimens in photomotor response bioassays. The system validation was showcased in a proof-of-concept neurotoxicity analysis.

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.