Dedicated Chamber for Multimodal In Vivo Imaging of Adult Zebrafish.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-03-16 DOI:10.1089/zeb.2021.0066

Steven Seeger, M. Zvolsky, Salim Melikov, Maja Frerkes, M. Rafecas

引用次数: 4

Abstract

In vivo imaging of adult zebrafish is challenging, particularly for dynamic or long acquisitions when using, for example, positron emission tomography, single photon emission computed tomography, computed tomography (CT), or magnetic resonance imaging (MRI). An aqueous environment is indispensable to ensure animal welfare, but commercial small-animal imaging chambers do not provide such conditions, as they are designed for rodents. In this study we present a dedicated flow-through chamber that includes fish immobilization and allows for the continuous supply of fresh water and anesthetics, as well as the removal of excretions. Both flow simulations and experiments, as well as first scans with MRI and CT, support the suitability of the chamber.

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成年斑马鱼体内多模态成像专用室。

成年斑马鱼的体内成像具有挑战性，特别是在使用正电子发射断层扫描、单光子发射计算机断层扫描、计算机断层扫描(CT)或磁共振成像(MRI)时进行动态或长时间采集。水环境是确保动物福利必不可少的，但商业小动物成像室不提供这样的条件，因为它们是为啮齿动物设计的。在这项研究中，我们提出了一个专用的流经腔，包括鱼类固定，允许连续供应淡水和麻醉剂，以及清除排泄物。流动模拟和实验，以及MRI和CT的首次扫描，都支持了该腔室的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.