A Simple Blood Vessel Imaging Protocol for Live Zebrafish Larva.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2022-10-01 DOI:10.1089/zeb.2022.0023

S Vinoth, Velanganni Selvaraj, Hemagowri Venkatasubramanian, Kirankumar Santhakumar

引用次数: 0

Abstract

Zebrafish larva is a simple model system to study blood vessel development because of its excellent optical properties. Current methods to study blood vessels in zebrafish involve utilizing either blood vessel-specific transgenic lines or specialized techniques such as microangiography and video capillaroscopy. In this study, we have developed a simple protocol using ImageJ to observe the blood vessels of live zebrafish larva at different developmental stages, namely 1- to 4-day postfertilization (dpf). To validate this protocol, we treated 1 dpf zebrafish embryos with 100 μM quercetin, and analyzed the impaired development of subintestinal vein in the quercetin-treated 3 dpf larvae.

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活体斑马鱼幼体的简单血管成像方案。

斑马鱼幼体具有优良的光学特性，是研究血管发育的简单模型系统。目前研究斑马鱼血管的方法包括利用血管特异性转基因系或专门技术，如微血管造影和视频毛细血管镜检查。在这项研究中，我们制定了一个简单的方案，使用ImageJ来观察不同发育阶段的活斑马鱼幼虫的血管，即受精后1- 4天(dpf)。为了验证这一方案，我们用100 μM槲皮素处理了1只dpf斑马鱼胚胎，并分析了槲皮素处理的3只dpf幼虫肠下静脉发育受损的情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.