Telomerase Activity in Androgenetic Rainbow Trout with Growth Deficiency and in Normally Developed Individuals.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2022-08-01 Epub Date: 2022-07-22 DOI:10.1089/zeb.2022.0012

Ligia Panasiak, Marcin Kuciński, Agata Błaszczyk, Konrad Ocalewicz

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引用次数: 1

Abstract

Role of telomerase in specimens with retarded growth (dwarfs) has not been thoroughly examined to date. Considering that some of the fish species show correlation between somatic growth and activity of telomerase, it has been tempting to assume that pattern of telomerase activity in specimens with retarded growth and these with normal growth rate may vary. In the present research, telomerase activity has been examined in liver, skin, and muscles in the androgenetic rainbow trout (Oncorhynchus mykiss) with growth deficiency and their normally developed siblings. Among the examined organs, the liver showed the highest telomerase activity in all studied fish, what may be linked to the enormous regeneration capacity of the liver tissue. Although dwarf specimens examined here displayed significantly lower body size and weight they did not exhibit any significant differences in the telomerase activity measured in liver and muscle when compared to the rainbow trout without growth deficiency. In turn, telomerase activity in skin was significantly upregulated in the normally developed androgenotes. The present study indicates that dwarfism in the androgenetic rainbow trout is neither associated with ceased telomerase activity nor its decrease throughout the ontogenetic development.

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生长缺陷和正常发育个体雄激素性虹鳟的端粒酶活性。

端粒酶在生长迟缓(侏儒)标本中的作用至今尚未得到彻底的研究。考虑到一些鱼类的体细胞生长与端粒酶活性之间存在相关性，人们很容易认为，生长迟缓的鱼类和生长正常的鱼类的端粒酶活性模式可能存在差异。在目前的研究中，端粒酶活性已被检测在肝脏，皮肤和肌肉的雄性发育虹鳟鱼(Oncorhynchus mykiss)生长缺陷及其正常发育的兄弟姐妹。在被检查的器官中，肝脏在所有被研究的鱼类中显示出最高的端粒酶活性，这可能与肝脏组织的巨大再生能力有关。虽然这里检查的侏儒标本显示出明显较低的体型和体重，但与没有生长缺陷的虹鳟相比，它们在肝脏和肌肉中测量的端粒酶活性没有任何显着差异。反过来，皮肤端粒酶活性在正常发育的雄激素中显着上调。目前的研究表明，雄性发育虹鳟鱼的侏儒症与端粒酶活性的停止或其在整个个体发育过程中的降低无关。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.